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Increased activity of chondroitin sulfate-synthesizing enzymes during proliferation of arterial smooth muscle cells.

Abstract：

:Cultured arterial smooth muscle cells incorporate [35S]sulfate into the extracellular chondroitin sulfate/dermatan sulfate containing proteoglycans at a higher rate in the phase of logarithmic growth than do non-dividing cells. The cell growth-dependent decrease in 35S incorporation with increasing cell density is accompanied by a decrease in the activity of chondroitin sulfate-synthesizing enzymes. The specific activity of xylosyl transferase, N-acetylgalactosaminyl transferase I and chondroitin sulfotransferase declines as the cells proceed from low to high densities. The corresponding correlation coefficients are 0.86, 0.91 and 0.89. The ratio of C-6OH/C-4OH sulfation of chondroitin shows a cell proliferation-dependent decrease indicating an inverse correlation of chondroitin 6-sulfotransferase and chondroitin 4-sulfotransferase activity. The observed changes in the expression of enzyme activities are thought to have some implications in the pathogenesis of arteriosclerosis, the initial stages of which are characterized by proliferation of arterial smooth muscle cells.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Hollmann J,Thiel J,Schmidt A,Buddecke E

doi

10.1016/0014-4827(86)90188-6

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

484-94

issue

2

eissn

0014-4827

issn

1090-2422

pii

0014-4827(86)90188-6

journal_volume

167

pub_type

杂志文章

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