A novel ex vivo tumor system identifies Src-mediated invasion and metastasis in mesenchymal tumor cells in non-small cell lung cancer.

Abstract:

:Lung cancer is the foremost cause of cancer related deaths in the U.S. It is a heterogeneous disease composed of genetically and phenotypically distinct tumor cells surrounded by heterotypic cells and extracellular matrix dynamically interacting with the tumor cells. Research in lung cancer is often restricted to patient-derived tumor specimens, in vitro cell cultures and limited animal models, which fail to capture the cellular or microenvironment heterogeneity of the tumor. Therefore, our knowledge is primarily focused on cancer-cell autonomous aberrations. For a fundamental understanding of lung cancer progression and an exploration of therapeutic options, we focused our efforts to develop an Ex Vivo Tumor platform to culture tumors in 3D matrices, which retains tumor cell heterogeneity arising due to in vivo selection pressure and environmental influences and recapitulate responses of tumor cells to external manipulations. To establish this model, implanted syngeneic murine tumors from a mutant KRAS/p53 model were harvested to yield multicellular tumor aggregates followed by culture in 3D extracellular matrices. Using this system, we identified Src signaling as an important driver of invasion and metastasis in lung cancer and demonstrate that EVTs are a robust experimental tool bridging the gap between conventional in vitro and in vivo models.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Padhye A,Ungewiss C,Fradette JJ,Rodriguez BL,Albritton JL,Miller JS,Gibbons DL

doi

10.1038/s41598-019-41301-2

subject

Has Abstract

pub_date

2019-03-20 00:00:00

pages

4819

issue

1

issn

2045-2322

pii

10.1038/s41598-019-41301-2

journal_volume

9

pub_type

杂志文章
  • Quantitative Investigation into the influence of intravenous fluids on human immune and cancer cell lines.

    abstract::The effect of intravenous fluids (IVF) has been investigated clinically through the assessment of post-treatment reactions. However, the responses to IVF vary from patient-to-patient. It is important to understand the response of IVF treatment to be able to provide optimal IVF care. Herein, we investigated the impact ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-61296-5

    authors: Karamahmutoglu H,Altay A,Vural S,Elitas M

    更新日期:2020-07-16 00:00:00

  • Fast and reliable advanced two-step pore-size analysis of biomimetic 3D extracellular matrix scaffolds.

    abstract::The tissue microenvironment is a major contributor to cellular functions, such as cell adhesion, migration and invasion. A critical physical parameter for determining the effect of the microenvironment on cellular functions is the average pore-size of filamentous scaffolds, such as 3D collagen fiber matrices, which ar...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44764-5

    authors: Fischer T,Hayn A,Mierke CT

    更新日期:2019-06-07 00:00:00

  • Complex patterns arise through spontaneous symmetry breaking in dense homogeneous networks of neural oscillators.

    abstract::There has been much interest in understanding collective dynamics in networks of brain regions due to their role in behavior and cognitive function. Here we show that a simple, homogeneous system of densely connected oscillators, representing the aggregate activity of local brain regions, can exhibit a rich variety of...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22074

    authors: Singh R,Menon SN,Sinha S

    更新日期:2016-02-26 00:00:00

  • Controllable thermal rectification realized in binary phase change composites.

    abstract::Phase transition is a natural phenomenon happened around our daily life, represented by the process from ice to water. While melting and solidifying at a certain temperature, a high heat of fusion is accompanied, classified as the latent heat. Phase change material (PCM) has been widely applied to store and release la...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08884

    authors: Chen R,Cui Y,Tian H,Yao R,Liu Z,Shu Y,Li C,Yang Y,Ren T,Zhang G,Zou R

    更新日期:2015-03-09 00:00:00

  • An agent-based algorithm resembles behaviour of tree-dwelling bats under fission-fusion dynamics.

    abstract::Utilization of computational approach in the study of social behaviour of animals is increasing and we attempted such an approach in our study of tree-dwelling bats. These bats live in highly dynamic fission-fusion societies that share multiple roosts in a common home range. The key behavioural component associated wi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72999-0

    authors: Zelenka J,Kasanický T,Budinská I,Kaňuch P

    更新日期:2020-10-08 00:00:00

  • Interplay between 3'-UTR polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene and the risk of ischemic stroke.

    abstract::Stroke incidence is a multifactorial disease and especially hyperhomocysteinemia is associated with a higher risk of stroke. Previous studies have reported a folate metabolism disorder associated with the MTHFR gene. We investigated four single nucleotide polymorphisms in the MTHFR 3'-UTR [2572 C > A (rs4846049), 4869...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12668-x

    authors: Kim JO,Park HS,Ryu CS,Shin JW,Kim J,Oh SH,Kim OJ,Kim NK

    更新日期:2017-09-29 00:00:00

  • Morphological and biomechanical characterization of immature and mature nasoseptal cartilage.

    abstract::Nasoseptal cartilage has been assumed to be isotropic, unlike the well-defined zonal organization of articular cartilage attributed to postnatal biomechanical loading. We know from clinical experience that malrotation of surgical nasoseptal cartilage grafts can lead to increased graft absorption. Other studies have al...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-48578-3

    authors: Jessop ZM,Zhang Y,Simoes IN,Al-Sabah A,Badiei N,Gazze SA,Francis L,Whitaker IS

    更新日期:2019-08-28 00:00:00

  • The stability of aluminium oxide monolayer and its interface with two-dimensional materials.

    abstract::The miniaturization of future electronic devices requires the knowledge of interfacial properties between two-dimensional channel materials and high-κ dielectrics in the limit of one atomic layer thickness. In this report, by combining particle-swarm optimization method with first-principles calculations, we present a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29221

    authors: Song TT,Yang M,Chai JW,Callsen M,Zhou J,Yang T,Zhang Z,Pan JS,Chi DZ,Feng YP,Wang SJ

    更新日期:2016-07-06 00:00:00

  • Body size and vocalization in primates and carnivores.

    abstract::A fundamental assumption in bioacoustics is that large animals tend to produce vocalizations with lower frequencies than small animals. This inverse relationship between body size and vocalization frequencies is widely considered to be foundational in animal communication, with prominent theories arguing that it playe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep41070

    authors: Bowling DL,Garcia M,Dunn JC,Ruprecht R,Stewart A,Frommolt KH,Fitch WT

    更新日期:2017-01-24 00:00:00

  • Sporadic Hirschsprung Disease: Mutational Spectrum and Novel Candidate Genes Revealed by Next-generation Sequencing.

    abstract::Hirschsprung disease (HSCR) is a common cause of functional colonic obstruction in children. The currently available genetic testing is often inadequate as it mainly focuses on RET and several other genes, accounting for only 15-20% of cases. To identify novel, potentially pathogenic variants, we isolated a panel of g...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章

    doi:10.1038/s41598-017-14835-6

    authors: Zhang Z,Li Q,Diao M,Liu N,Cheng W,Xiao P,Zou J,Su L,Yu K,Wu J,Li L,Jiang Q

    更新日期:2017-11-01 00:00:00

  • Prognostic value of platelet to lymphocyte ratio in hepatocellular carcinoma: a meta-analysis.

    abstract::This study was designed to evaluate the prognostic value of platelet to lymphocyte ratio (PLR) in hepatocellular carcinoma (HCC). A comprehensive literature search for relevant studies was performed in Web of science, Embase and Pubmed. A total of nine studies with 2017 patients were included in this meta-analysis, an...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/srep35378

    authors: Ma W,Zhang P,Qi J,Gu L,Zang M,Yao H,Shi X,Wang C,Jiang Y

    更新日期:2016-10-14 00:00:00

  • Neural oscillations associated with auditory duration maintenance in working memory.

    abstract::The neural representation of auditory duration remains unknown. Here, we used electroencephalogram (EEG) recordings to investigate neural oscillations during the maintenance of auditory duration in working memory (WM). EEG analyses indicated that the auditory duration length was not associated with changes in the thet...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06078-2

    authors: Yu X,Chen Y,Qiu J,Li X,Huang X

    更新日期:2017-07-18 00:00:00

  • Exploring source differences on diet-tissue discrimination factors in the analysis of stable isotope mixing models.

    abstract::Stable isotope mixing models are regularly used to provide probabilistic estimates of source contributions to dietary mixtures. Whilst Bayesian implementations of isotope mixing models have become prominent, the use of appropriate diet-tissue discrimination factors (DTDFs) remains as the least resolved aspect. The DTD...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73019-x

    authors: Kadye WT,Redelinghuys S,Parnell AC,Booth AJ

    更新日期:2020-09-25 00:00:00

  • Ruthenium(II) complexes with 6-methyl-2-thiouracil selectively reduce cell proliferation, cause DNA double-strand break and trigger caspase-mediated apoptosis through JNK/p38 pathways in human acute promyelocytic leukemia cells.

    abstract::Ruthenium(II) complexes with 6-methyl-2-thiouracil cis-[Ru(6m2tu)2(PPh3)2] (1) and [Ru(6m2tu)2(dppb)] (2) (where PPh3 = triphenylphosphine; dppb = 1,4-bis(diphenylphosphino)butane; and 6m2tu = 6-methyl-2-thiouracil) are potent cytotoxic agents and able to bind DNA. The aim of this study was to evaluate in vitro cellul...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47914-x

    authors: Bomfim LM,de Araujo FA,Dias RB,Sales CBS,Rocha CAG,Correa RS,Soares MBP,Batista AA,Bezerra DP

    更新日期:2019-08-07 00:00:00

  • Room temperature self-assembled growth of vertically aligned columnar copper oxide nanocomposite thin films on unmatched substrates.

    abstract::In this work, we report the self-assembled growth of vertically aligned columnar Cu2O + Cu4O3 nanocomposite thin films on glass and silicon substrates by reactive sputtering at room temperature. Microstructure analyses show that each phase in nanocomposite films has the columnar growth along the whole thickness, while...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10540-6

    authors: Wang Y,Ghanbaja J,Bruyère S,Soldera F,Horwat D,Mücklich F,Pierson JF

    更新日期:2017-09-11 00:00:00

  • Activity-dependent changes in synaptic protein complex composition are consistent in different detergents despite differential solubility.

    abstract::At the post-synaptic density (PSD), large protein complexes dynamically form and dissociate in response to synaptic activity, comprising the biophysical basis for learning and memory. The use of detergents to isolate the PSD and release its membrane-associated proteins complicates studies of these activity-dependent p...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-46690-y

    authors: Lautz JD,Gniffke EP,Brown EA,Immendorf KB,Mendel RD,Smith SEP

    更新日期:2019-07-26 00:00:00

  • Current controlled switching of impedance in magnetic conductor with tilted anisotropy easy axis and its applications.

    abstract::We present a concept and prototype of a memory element based on current driven magneto-impedance (MI) effect that stores the binary data (0, 1) as the orientation of the magnetization. The magnetization orientation in the surface layer with tilted anisotropy easy axis can be switched controllably between two stable st...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36180

    authors: Ipatov M,Zhukova V,Zhukov A,Gonzalez J

    更新日期:2016-10-26 00:00:00

  • Tolerance and responsive gene expression of Sogatella furcifera under extreme temperature stresses are altered by its vectored plant virus.

    abstract::Southern rice black-streaked dwarf virus (SRBSDV), a newly emerged fijivirus causing great loss to rice production in eastern and southeastern Asian countries in recent years, is efficiently transmitted by a rice pest, white-backed planthopper (WBPH, Sogatella furcifera) in a persistent, circulative propagative manner...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31521

    authors: Xu D,Zhong T,Feng W,Zhou G

    更新日期:2016-08-17 00:00:00

  • Genome-wide Identification of PP2C Genes and Their Expression Profiling in Response to Drought and Cold Stresses in Medicago truncatula.

    abstract::Type 2 C protein phosphatases (PP2Cs) represent the major group of protein phosphatases in plants and play important roles in various plant processes. In this study, 94 MtPP2C genes were identified from Medicago truncatula and further phylogenetically classified into 13 subfamilies, as supported by exon-intron organiz...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-29627-9

    authors: Yang Q,Liu K,Niu X,Wang Q,Wan Y,Yang F,Li G,Wang Y,Wang R

    更新日期:2018-08-27 00:00:00

  • Lipocalin 2 Does Not Play A Role in Celastrol-Mediated Reduction in Food Intake and Body Weight.

    abstract::Celastrol is a leptin-sensitizing agent with profound anti-obesity effects in diet-induced obese (DIO) mice. However, the genes and pathways that mediate celastrol-induced leptin sensitization have not been fully understood. By comparing the hypothalamic transcriptomes of celastrol and vehicle-treated DIO mice, we ide...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49151-8

    authors: Feng X,Guan D,Auen T,Choi JW,Salazar-Hernandez MA,Faruk F,Copps KD,Ozcan U

    更新日期:2019-09-05 00:00:00

  • TGF-β regulates phosphorylation and stabilization of Sox9 protein in chondrocytes through p38 and Smad dependent mechanisms.

    abstract::Members of the TGF-β superfamily are important regulators of chondrocyte function. Sox9, a key transcriptional regulator of chondrogenesis, is required for TGF-β-mediated regulation of specific cartilage genes. TGF-β can signal through a canonical, Smad-mediated pathway or non-conical pathways, including p38. Here we ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38616

    authors: Coricor G,Serra R

    更新日期:2016-12-08 00:00:00

  • Generation of PDGFRα+ Cardioblasts from Pluripotent Stem Cells.

    abstract::Isolating actively proliferating cardioblasts is the first crucial step for cardiac regeneration through cell implantation. However, the origin and identity of putative cardioblasts are still unclear. Here, we uncover a novel class of cardiac lineage cells, PDGFRα+Flk1- cardioblasts (PCBs), from mouse and human plurip...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep41840

    authors: Hong SP,Song S,Cho SW,Lee S,Koh BI,Bae H,Kim KH,Park JS,Do HS,Im I,Heo HJ,Ko TH,Park JH,Youm JB,Kim SJ,Kim I,Han J,Han YM,Koh GY

    更新日期:2017-02-06 00:00:00

  • Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection.

    abstract::Gut bacteria influence the development of different pathologies caused by bacteria, fungi and parasitoids in insects. Wax moth larvae became more susceptible to fungal infections after envenomation by the ectoparasitoid Habrobracon hebetor. In addition, spontaneous bacterioses occurred more often in envenomated larvae...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40301-6

    authors: Polenogova OV,Kabilov MR,Tyurin MV,Rotskaya UN,Krivopalov AV,Morozova VV,Mozhaitseva K,Kryukova NA,Alikina T,Kryukov VY,Glupov VV

    更新日期:2019-03-08 00:00:00

  • Purification, Characterization and Mechanistic Evaluation of Angiotensin Converting Enzyme Inhibitory Peptides Derived from Zizyphus Jujuba Fruit.

    abstract::The synthetic Angiotensin Converting Enzyme (ACE) inhibitors have side effects and hence demands for natural ACE inhibitors have been rising. The aim of this study is to purify and introduce natural ACE inhibitors extracted from Zizyphus jujuba fruits. Proteins from Zizyphus jujuba were lysed by trypsin, papain and th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60972-w

    authors: Memarpoor-Yazdi M,Zare-Zardini H,Mogharrab N,Navapour L

    更新日期:2020-03-04 00:00:00

  • Analysis and modeling of Fano resonances using equivalent circuit elements.

    abstract::Fano resonance presents an asymmetric line shape formed by an interference of a continuum coupled with a discrete autoionized state. In this paper, we show several simple circuits for Fano resonances from the stable-input impedance mechanism, where the elements consisting of inductors and capacitors are formulated for...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31884

    authors: Lv B,Li R,Fu J,Wu Q,Zhang K,Chen W,Wang Z,Ma R

    更新日期:2016-08-22 00:00:00

  • Land subsidence contributions to relative sea level rise at tide gauge Galveston Pier 21, Texas.

    abstract::Relative sea level rise at tide gauge Galveston Pier 21, Texas, is the combination of absolute sea level rise and land subsidence. We estimate subsidence rates of 3.53 mm/a during 1909-1937, 6.08 mm/a during 1937-1983, and 3.51 mm/a since 1983. Subsidence attributed to aquifer-system compaction accompanying groundwate...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74696-4

    authors: Liu Y,Li J,Fasullo J,Galloway DL

    更新日期:2020-10-21 00:00:00

  • Intelligent Metal-Phenolic Metallogels as Dressings for Infected Wounds.

    abstract::In this study, we report a metallogel developed based on metal-phenolic coordination of natural low-cost polyphenolic molecule and metal ions. Gelation occurs by mixing tannic acid (TA) and group (IV) titanium ions (TiIV) to form TA-TiIV gel. The TA-TiIV gel exhibits good capability to incorporate diverse metal ions b...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47978-9

    authors: Anh HTP,Huang CM,Huang CJ

    更新日期:2019-08-09 00:00:00

  • Author Correction: Genomewide Study of Epigenetic Biomarkers of Opioid Dependence in European- American Women.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-019-55022-z

    authors: Montalvo-Ortiz JL,Cheng Z,Kranzler HR,Zhang H,Gelernter J

    更新日期:2019-12-05 00:00:00

  • Prognostic significance of Rab27 expression in solid cancer: a systematic review and meta-analysis.

    abstract::Rab27 is an essential molecule of vesicle fusion and trafficking in exosome secretion process, which plays important roles in cancer progression and metastasis. Recent studies reported that Rab27 expression is also associated with cancer prognosis. Therefore, we performed a meta-analysis to reveal the prognostic signi...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-020-71104-9

    authors: Koh HM,Jang BG,Kim DC

    更新日期:2020-08-24 00:00:00

  • Linearly polarized GHz magnetization dynamics of spin helix modes in the ferrimagnetic insulator Cu2OSeO3.

    abstract::Linear dichroism - the polarization dependent absorption of electromagnetic waves- is routinely exploited in applications as diverse as structure determination of DNA or polarization filters in optical technologies. Here filamentary absorbers with a large length-to-width ratio are a prerequisite. For magnetization dyn...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07020-2

    authors: Stasinopoulos I,Weichselbaumer S,Bauer A,Waizner J,Berger H,Garst M,Pfleiderer C,Grundler D

    更新日期:2017-08-01 00:00:00