Abstract:
:Lung cancer is the foremost cause of cancer related deaths in the U.S. It is a heterogeneous disease composed of genetically and phenotypically distinct tumor cells surrounded by heterotypic cells and extracellular matrix dynamically interacting with the tumor cells. Research in lung cancer is often restricted to patient-derived tumor specimens, in vitro cell cultures and limited animal models, which fail to capture the cellular or microenvironment heterogeneity of the tumor. Therefore, our knowledge is primarily focused on cancer-cell autonomous aberrations. For a fundamental understanding of lung cancer progression and an exploration of therapeutic options, we focused our efforts to develop an Ex Vivo Tumor platform to culture tumors in 3D matrices, which retains tumor cell heterogeneity arising due to in vivo selection pressure and environmental influences and recapitulate responses of tumor cells to external manipulations. To establish this model, implanted syngeneic murine tumors from a mutant KRAS/p53 model were harvested to yield multicellular tumor aggregates followed by culture in 3D extracellular matrices. Using this system, we identified Src signaling as an important driver of invasion and metastasis in lung cancer and demonstrate that EVTs are a robust experimental tool bridging the gap between conventional in vitro and in vivo models.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Padhye A,Ungewiss C,Fradette JJ,Rodriguez BL,Albritton JL,Miller JS,Gibbons DLdoi
10.1038/s41598-019-41301-2subject
Has Abstractpub_date
2019-03-20 00:00:00pages
4819issue
1issn
2045-2322pii
10.1038/s41598-019-41301-2journal_volume
9pub_type
杂志文章abstract::The effect of intravenous fluids (IVF) has been investigated clinically through the assessment of post-treatment reactions. However, the responses to IVF vary from patient-to-patient. It is important to understand the response of IVF treatment to be able to provide optimal IVF care. Herein, we investigated the impact ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61296-5
更新日期:2020-07-16 00:00:00
abstract::The tissue microenvironment is a major contributor to cellular functions, such as cell adhesion, migration and invasion. A critical physical parameter for determining the effect of the microenvironment on cellular functions is the average pore-size of filamentous scaffolds, such as 3D collagen fiber matrices, which ar...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44764-5
更新日期:2019-06-07 00:00:00
abstract::There has been much interest in understanding collective dynamics in networks of brain regions due to their role in behavior and cognitive function. Here we show that a simple, homogeneous system of densely connected oscillators, representing the aggregate activity of local brain regions, can exhibit a rich variety of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22074
更新日期:2016-02-26 00:00:00
abstract::Phase transition is a natural phenomenon happened around our daily life, represented by the process from ice to water. While melting and solidifying at a certain temperature, a high heat of fusion is accompanied, classified as the latent heat. Phase change material (PCM) has been widely applied to store and release la...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08884
更新日期:2015-03-09 00:00:00
abstract::Utilization of computational approach in the study of social behaviour of animals is increasing and we attempted such an approach in our study of tree-dwelling bats. These bats live in highly dynamic fission-fusion societies that share multiple roosts in a common home range. The key behavioural component associated wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72999-0
更新日期:2020-10-08 00:00:00
abstract::Stroke incidence is a multifactorial disease and especially hyperhomocysteinemia is associated with a higher risk of stroke. Previous studies have reported a folate metabolism disorder associated with the MTHFR gene. We investigated four single nucleotide polymorphisms in the MTHFR 3'-UTR [2572 C > A (rs4846049), 4869...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12668-x
更新日期:2017-09-29 00:00:00
abstract::Nasoseptal cartilage has been assumed to be isotropic, unlike the well-defined zonal organization of articular cartilage attributed to postnatal biomechanical loading. We know from clinical experience that malrotation of surgical nasoseptal cartilage grafts can lead to increased graft absorption. Other studies have al...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48578-3
更新日期:2019-08-28 00:00:00
abstract::The miniaturization of future electronic devices requires the knowledge of interfacial properties between two-dimensional channel materials and high-κ dielectrics in the limit of one atomic layer thickness. In this report, by combining particle-swarm optimization method with first-principles calculations, we present a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29221
更新日期:2016-07-06 00:00:00
abstract::A fundamental assumption in bioacoustics is that large animals tend to produce vocalizations with lower frequencies than small animals. This inverse relationship between body size and vocalization frequencies is widely considered to be foundational in animal communication, with prominent theories arguing that it playe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41070
更新日期:2017-01-24 00:00:00
abstract::Hirschsprung disease (HSCR) is a common cause of functional colonic obstruction in children. The currently available genetic testing is often inadequate as it mainly focuses on RET and several other genes, accounting for only 15-20% of cases. To identify novel, potentially pathogenic variants, we isolated a panel of g...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-017-14835-6
更新日期:2017-11-01 00:00:00
abstract::This study was designed to evaluate the prognostic value of platelet to lymphocyte ratio (PLR) in hepatocellular carcinoma (HCC). A comprehensive literature search for relevant studies was performed in Web of science, Embase and Pubmed. A total of nine studies with 2017 patients were included in this meta-analysis, an...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/srep35378
更新日期:2016-10-14 00:00:00
abstract::The neural representation of auditory duration remains unknown. Here, we used electroencephalogram (EEG) recordings to investigate neural oscillations during the maintenance of auditory duration in working memory (WM). EEG analyses indicated that the auditory duration length was not associated with changes in the thet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06078-2
更新日期:2017-07-18 00:00:00
abstract::Stable isotope mixing models are regularly used to provide probabilistic estimates of source contributions to dietary mixtures. Whilst Bayesian implementations of isotope mixing models have become prominent, the use of appropriate diet-tissue discrimination factors (DTDFs) remains as the least resolved aspect. The DTD...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73019-x
更新日期:2020-09-25 00:00:00
abstract::Ruthenium(II) complexes with 6-methyl-2-thiouracil cis-[Ru(6m2tu)2(PPh3)2] (1) and [Ru(6m2tu)2(dppb)] (2) (where PPh3 = triphenylphosphine; dppb = 1,4-bis(diphenylphosphino)butane; and 6m2tu = 6-methyl-2-thiouracil) are potent cytotoxic agents and able to bind DNA. The aim of this study was to evaluate in vitro cellul...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47914-x
更新日期:2019-08-07 00:00:00
abstract::In this work, we report the self-assembled growth of vertically aligned columnar Cu2O + Cu4O3 nanocomposite thin films on glass and silicon substrates by reactive sputtering at room temperature. Microstructure analyses show that each phase in nanocomposite films has the columnar growth along the whole thickness, while...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10540-6
更新日期:2017-09-11 00:00:00
abstract::At the post-synaptic density (PSD), large protein complexes dynamically form and dissociate in response to synaptic activity, comprising the biophysical basis for learning and memory. The use of detergents to isolate the PSD and release its membrane-associated proteins complicates studies of these activity-dependent p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46690-y
更新日期:2019-07-26 00:00:00
abstract::We present a concept and prototype of a memory element based on current driven magneto-impedance (MI) effect that stores the binary data (0, 1) as the orientation of the magnetization. The magnetization orientation in the surface layer with tilted anisotropy easy axis can be switched controllably between two stable st...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36180
更新日期:2016-10-26 00:00:00
abstract::Southern rice black-streaked dwarf virus (SRBSDV), a newly emerged fijivirus causing great loss to rice production in eastern and southeastern Asian countries in recent years, is efficiently transmitted by a rice pest, white-backed planthopper (WBPH, Sogatella furcifera) in a persistent, circulative propagative manner...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31521
更新日期:2016-08-17 00:00:00
abstract::Type 2 C protein phosphatases (PP2Cs) represent the major group of protein phosphatases in plants and play important roles in various plant processes. In this study, 94 MtPP2C genes were identified from Medicago truncatula and further phylogenetically classified into 13 subfamilies, as supported by exon-intron organiz...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29627-9
更新日期:2018-08-27 00:00:00
abstract::Celastrol is a leptin-sensitizing agent with profound anti-obesity effects in diet-induced obese (DIO) mice. However, the genes and pathways that mediate celastrol-induced leptin sensitization have not been fully understood. By comparing the hypothalamic transcriptomes of celastrol and vehicle-treated DIO mice, we ide...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49151-8
更新日期:2019-09-05 00:00:00
abstract::Members of the TGF-β superfamily are important regulators of chondrocyte function. Sox9, a key transcriptional regulator of chondrogenesis, is required for TGF-β-mediated regulation of specific cartilage genes. TGF-β can signal through a canonical, Smad-mediated pathway or non-conical pathways, including p38. Here we ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38616
更新日期:2016-12-08 00:00:00
abstract::Isolating actively proliferating cardioblasts is the first crucial step for cardiac regeneration through cell implantation. However, the origin and identity of putative cardioblasts are still unclear. Here, we uncover a novel class of cardiac lineage cells, PDGFRα+Flk1- cardioblasts (PCBs), from mouse and human plurip...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41840
更新日期:2017-02-06 00:00:00
abstract::Gut bacteria influence the development of different pathologies caused by bacteria, fungi and parasitoids in insects. Wax moth larvae became more susceptible to fungal infections after envenomation by the ectoparasitoid Habrobracon hebetor. In addition, spontaneous bacterioses occurred more often in envenomated larvae...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40301-6
更新日期:2019-03-08 00:00:00
abstract::The synthetic Angiotensin Converting Enzyme (ACE) inhibitors have side effects and hence demands for natural ACE inhibitors have been rising. The aim of this study is to purify and introduce natural ACE inhibitors extracted from Zizyphus jujuba fruits. Proteins from Zizyphus jujuba were lysed by trypsin, papain and th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60972-w
更新日期:2020-03-04 00:00:00
abstract::Fano resonance presents an asymmetric line shape formed by an interference of a continuum coupled with a discrete autoionized state. In this paper, we show several simple circuits for Fano resonances from the stable-input impedance mechanism, where the elements consisting of inductors and capacitors are formulated for...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31884
更新日期:2016-08-22 00:00:00
abstract::Relative sea level rise at tide gauge Galveston Pier 21, Texas, is the combination of absolute sea level rise and land subsidence. We estimate subsidence rates of 3.53 mm/a during 1909-1937, 6.08 mm/a during 1937-1983, and 3.51 mm/a since 1983. Subsidence attributed to aquifer-system compaction accompanying groundwate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74696-4
更新日期:2020-10-21 00:00:00
abstract::In this study, we report a metallogel developed based on metal-phenolic coordination of natural low-cost polyphenolic molecule and metal ions. Gelation occurs by mixing tannic acid (TA) and group (IV) titanium ions (TiIV) to form TA-TiIV gel. The TA-TiIV gel exhibits good capability to incorporate diverse metal ions b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47978-9
更新日期:2019-08-09 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-55022-z
更新日期:2019-12-05 00:00:00
abstract::Rab27 is an essential molecule of vesicle fusion and trafficking in exosome secretion process, which plays important roles in cancer progression and metastasis. Recent studies reported that Rab27 expression is also associated with cancer prognosis. Therefore, we performed a meta-analysis to reveal the prognostic signi...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-020-71104-9
更新日期:2020-08-24 00:00:00
abstract::Linear dichroism - the polarization dependent absorption of electromagnetic waves- is routinely exploited in applications as diverse as structure determination of DNA or polarization filters in optical technologies. Here filamentary absorbers with a large length-to-width ratio are a prerequisite. For magnetization dyn...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07020-2
更新日期:2017-08-01 00:00:00