Abstract:
:The use of biochemical signaling to derive smooth muscle cells (SMCs) from mesenchymal stem cells (MSCs) has been explored, but the induction of a fully functional SMC phenotype remains to be a major challenge. Cell morphology has been shown to regulate MSC differentiation into various lineages, including SMCs. We engineered substrates with microgrooves to induce cell elongation to study the mechanism underlying the MSC shape modulation in SMC differentiation. In comparison to those on flat substrates, MSCs cultured on engineered substrates were elongated with increased aspect ratios for both cell body and nucleus, as well as augmented cytoskeletal tensions. Biochemical studies indicated that the microgroove-elongated cells expressed significantly higher levels of SMC markers. MicroRNA analyses showed that up-regulation of miR-145 and the consequent repression of KLF4 in these elongated cells promoted MSC-to-SMC differentiation. Rho/ROCK inhibitions, which impair cytoskeletal tension, attenuated cell and nuclear elongations and disrupted the miR-145/KLF4 regulation for SMC differentiation. Furthermore, cell traction force measurements showed that miR-145 is essential for the functional contractility in the microgroove-induced SMC differentiation. Collectively, our findings demonstrate that, through a Rho-ROCK/miR-145/KLF4 pathway, the elongated cell shape serves as a decisive geometric cue to direct MSC differentiation into functional SMCs.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Yeh YT,Wei J,Thorossian S,Nguyen K,Hoffman C,Del Álamo JC,Serrano R,Li YJ,Wang KC,Chien Sdoi
10.1016/j.biomaterials.2019.03.003subject
Has Abstractpub_date
2019-06-01 00:00:00pages
59-69eissn
0142-9612issn
1878-5905pii
S0142-9612(19)30127-9journal_volume
204pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition s...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.03.011
更新日期:2014-06-01 00:00:00
abstract::The use of artificial tissues in regenerative medicine is limited due to hypoxia. As a strategy to overcome this drawback, we have shown that photosynthetic biomaterials can produce and provide oxygen independently of blood perfusion by generating chimeric animal-plant tissues during dermal regeneration. In this work,...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.10.014
更新日期:2016-01-01 00:00:00
abstract::Surface properties of poly (D,L-lactide) (PDLLA) were modified by combining plasma treatment and collagen modification. The changes of surface properties were characterized by contact angles, surface energy, X-ray photoelectron spectra and scanning electron microscopy. The mouse 3T3 fibroblasts were used as model cell...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00400-8
更新日期:2002-06-01 00:00:00
abstract::Polystyrene and phenylated polyacrolein microspheres of different diameters, as well as modified cellulose microspheres with different surface charges, were prepared in order to study the size and surface charge effect on their phagocytosis by mouse peritoneal macrophages. It was found that the maximal phagocytosis of...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(88)90033-6
更新日期:1988-07-01 00:00:00
abstract::A major cross-cutting problem for glioma therapy is the poor extravasation and penetration of the payload drug in target glioma parenchyma. Here, to overcome these obstacles, a tumor vessel recognizing and tumor penetrating system is developed by functionalizating the poly (ethyleneglycol)-poly (L-lactic-co-glycolic a...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.01.082
更新日期:2014-05-01 00:00:00
abstract::Polysaccharides are increasingly being used as biomaterials for tissue engineering and regenerative medicine. Quantitative analysis of gene expression from cells in three-dimensional (3D) scaffolds requires extraction of messenger RNA, which is complicated in polysaccharide materials by ionic complexing between nuclei...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.11.024
更新日期:2010-03-01 00:00:00
abstract::The creation of 3D scaffolds that mimic the structure of physiological tissue required for normal cell function is a major bioengineering challenge. For corneal stroma reconstruction this necessitates the creation of a stroma-like scaffold consisting of a stack of orthogonally disposed sheets of aligned collagen fibri...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2007.05.024
更新日期:2007-10-01 00:00:00
abstract::Mononuclear phagocyte system is the first target for injected nanomaterials. Monocytes/macrophages are the central actors for trafficking and clearance of magnetic nanoparticles in vivo. However the fate of nanosized magnetic label is an ongoing issue. Here we demonstrate that the monocyte/macrophage system shows a co...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.05.062
更新日期:2010-09-01 00:00:00
abstract::The blood-brain barrier (BBB) prevents entry of neurotoxic substances but also that of drugs into the brain. Here, the paracellular barrier is formed by tight junctions (TJs) with claudin-5 (Cldn5) being the main sealing constituent. Transient BBB opening by targeting Cldn5 could improve paracellular drug delivery. Th...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2018.01.028
更新日期:2018-04-01 00:00:00
abstract::Here we document the complete process of bioresorption and bone replacement of rods made of forged composites of unsintered hydroxyapatite particles/poly l-lactide (F-u-HA/PLLA) implanted in the femoral medullary cavities of rabbits. Bioresorption, osteoconductive bioactivity and bone replacement were compared in thre...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.02.016
更新日期:2005-09-01 00:00:00
abstract::A silicone-based prepolymer was used as the major component for the fabrication of novel ocular devices. Gentamicin sulphate was used as a probe drug, due to its high aqueous solubility and widespread application. Mechanical properties of films containing the drug before and after gamma sterilization and exposure to h...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(90)90035-o
更新日期:1990-11-01 00:00:00
abstract::The differentiation of odontoblasts is initiated by the organization of differentiating ameloblasts during tooth formation. However, the exact roles of ameloblast-derived factors in odontoblast differentiation have not yet been characterized. We investigated the effects of preameloblast-conditioned medium (PA-CM) on t...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.09.007
更新日期:2011-12-01 00:00:00
abstract::In spite of good prospects for bone morphogenetic proteins (BMP) applications, an ideal carrier system for BMPs has not yet been identified. The purpose of this study was to evaluate the osteogenic effect of a fibrin-fibronectin sealing system (FFSS) combined with beta-tricalcium phosphate (beta-TCP) as a carrier syst...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2006.02.045
更新日期:2006-07-01 00:00:00
abstract::The ageing behaviour of ultra-high molecular weight polyethylene (UHMWPE) has been studied following gamma irradiation (25 or 40 kGy) in air. Accelerated ageing procedures used elevated temperature (70 degrees C) and/or pressurised oxygen (5bar). Shelf-aged UHMWPE was also studied. The variation in surface density and...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00083-7
更新日期:2002-01-01 00:00:00
abstract::In vivo and in vitro degradation of high molecular weight poly(L-lactide) used for internal bone fixation has been investigated. Within 3 months as-polymerized, microporous PLLA (Mv = 6.8-9.5 X 10(5] exhibited a massive strength-loss (sigma b = 68-75 MPa to sigma b = 4 MPa) and decrease of Mv (90-95%). At week 39, the...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(87)90121-9
更新日期:1987-07-01 00:00:00
abstract::Five Garren-Edwards Gastric Bubbles were characterized, following up to 4 months use in vivo, using size exclusion chromatography, differential scanning calorimetry and attenuated total reflectance infrared spectroscopy. These techniques show that the material used to construct the bubble is probably an aromatic polye...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(92)90191-p
更新日期:1992-01-01 00:00:00
abstract::Multilayer nanofilms, formed by the layer-by-layer (LbL) adsorption of positively and negatively charged polyelectrolytes, are promising substrates for tissue engineering. We investigate here the attachment and function of hepatic cells on multilayer films in terms of film composition, terminal layer, rigidity, charge...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.06.027
更新日期:2008-10-01 00:00:00
abstract::Targeted tumor imaging and efficient specific gene delivery in vivo has been one of the main challenges in gene-based cancer diagnosis and therapy. Herein, we engineered a citric acid-based polymer with intrinsical photoluminescence and gene loading capacity to achieve targeted delivery of siRNA and tumor imaging in v...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2019.01.045
更新日期:2019-04-01 00:00:00
abstract::Tissue engineering requires the use of polymeric scaffolds that mimic many roles of extracellular matrices (ECM) in the body. Controlling cell-scaffold interactions is one of the most critical parameters for regulating cell phenotype in tissue engineering, and a peptide with the sequence of RGD has been widely exploit...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.03.063
更新日期:2010-07-01 00:00:00
abstract::This work exploits the thermal responsive phase behavior of hydroxypropylcellulose to produce 3D interconnected macroporous hydrogels in aqueous environment. Hydroxypropylcellulose was modified with allyl isocyanate, and their temperature mediated phase behavior was studied as a function of degree of modification (DS)...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.07.059
更新日期:2010-11-01 00:00:00
abstract::The ultra-short pulse microwave could excite to the strong thermoacoustic (TA) shock wave and deeply penetrate in the biological tissues. Based on this, we developed a novel deep-seated tumor therapy modality with mitochondria-targeting single wall carbon nanotubes (SWNTs) as microwave absorbing agents, which act effi...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.10.028
更新日期:2016-01-01 00:00:00
abstract::Poly(vinyl alcohol)-heparin hydrogels with varying water content were synthesized for use as sensor catheter membranes. Films were cast from aqueous mixtures of poly(vinyl alcohol) (PVA), a photosensitive cross-linker p-diazonium diphenyl amine polymer (PA), glutaraldehyde (GA) and heparin. After drying, the films wer...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(91)90134-v
更新日期:1991-01-01 00:00:00
abstract::The shelf life of implantable materials has rarely been addressed. We determined whether osteoconductivity of titanium is stable over time. Rat bone marrow-derived osteoblasts were cultured on new titanium disks (immediately after acid-etching), 3-day-old (stored after acid-etching for 3 days in dark ambient condition...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.06.040
更新日期:2009-10-01 00:00:00
abstract::A newly developed bone equivalent hydroxyapatite was derived from veterinary bone (VHAP). Sections of 1 cm of six rabbit mandibles were equally replaced by this VHAP graft. Radiological studies by X-ray were performed pre-operatively, immediately, and 1, 2 and 3 months post-operatively. The graft host-bone interface w...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(94)90211-9
更新日期:1994-06-01 00:00:00
abstract::Current prosthetic small diameter vascular grafts show poor long-term patency rates, leading to the pursuit of a biological alternative. Hyaff-11 is a hyaluronan-based biodegradable polymer developed for tissue-engineering applications. This study aimed to determine whether human vascular endothelial cells attach to H...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2004.02.002
更新日期:2004-12-01 00:00:00
abstract::It was reported that nanoparticles with polysorbate 80 (Tween 80, T-80) coating represented tools used for delivering drugs to brain. Nevertheless, disputations were once aroused for some complications. Aimed to have a better understanding of the specific role of T-80 coating on nanoparticles and simplify the problem,...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.09.087
更新日期:2004-07-01 00:00:00
abstract::Dental amalgam prepared from high copper and conventional alloys was evaluated for its compressive strength as a function of deformation rate. Results showed that the compressive strength rose to a maximum as deformation rate increased and then began to fall at higher deformation rates. Crack propagation predominated ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(86)90046-3
更新日期:1986-07-01 00:00:00
abstract::Experimental and histological studies on the reaction of surrounding gingiva to permucosal implants of dense calcium hydroxyapatite were carried out in dogs. Epithelial attachment and connection of supra-alveolar collagen fibres to the implant surface seemed to form a biological seal around implants similar to that ar...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(84)90018-8
更新日期:1984-07-01 00:00:00
abstract::In the present study, we tested the hypothesis that electroporation-mediated transfer of Runx2, Osterix, or both genes enhances the in vitro and in vivo osteogenesis from adipose stem cells (ASCs). ASCs were transfected with Runx2, Osterix, or both genes using electroporation, and further cultured in monolayer or in P...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.09.042
更新日期:2011-01-01 00:00:00
abstract::Electroconductive hydrogels (ECHs) are composite biomaterials that bring together the redox switching and electrical properties of inherently conductive electroactive polymers (CEPs) with the facile small molecule transport, high hydration levels and biocompatibility of cross-linked hydrogels. General methods for the ...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2009.12.052
更新日期:2010-04-01 00:00:00