Abstract:
:Solid-state femtosecond lasers have stimulated the broad adoption of multiphoton microscopy in the modern laboratory. However, these devices remain costly. Fiber lasers offer promise as a means to inexpensively produce ultrashort pulses of light suitable for nonlinear microscopy in compact, robust and portable devices. Although encouraging, the initial methods reported in the biomedical engineering community to construct home-built femtosecond fiber laser systems overlooked fundamental aspects that compromised performance and misrepresented the significant financial and intellectual investments required to build these devices. Here, we present a practical protocol to fabricate an all-normal-dispersion ytterbium (Yb)-doped femtosecond fiber laser oscillator using commercially-available parts (plus standard optical components and extra-cavity accessories) as well as basic fiber splicing and laser pulse characterization equipment. We also provide a synthesis of established protocols in the laser physics community, but often overlooked in other fields, to verify true versus seemingly (partial or noise-like) mode-locked performance. The approaches described here make custom fabrication of femtosecond fiber lasers more accessible to a wide range of investigators and better represent the investments required for the proper laser design, fabrication and operation.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Davoudzadeh N,Ducourthial G,Spring BQdoi
10.1038/s41598-019-40871-5subject
Has Abstractpub_date
2019-03-12 00:00:00pages
4233issue
1issn
2045-2322pii
10.1038/s41598-019-40871-5journal_volume
9pub_type
杂志文章abstract::We have observed that one in three patients admitted to the Neurological Rehabilitation Ward for Children and Adolescents due to a syndrome or disease associated with neurodysfunction is short of stature for their age. In order to identify the relationship between growth defects (short stature) and syndromes or diseas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52918-8
更新日期:2019-11-11 00:00:00
abstract::Self-organization and pattern formation in network-organized systems emerges from the collective activation and interaction of many interconnected units. A striking feature of these non-equilibrium structures is that they are often localized and robust: only a small subset of the nodes, or cell assembly, is activated....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21360
更新日期:2016-02-17 00:00:00
abstract::Both plant neighbourhood composition and drought have well-known independent effects on insect herbivore performance, but their interactive effects remain elusive. In this study we performed a laboratory experiment to investigate the independent and combined effects of plant neighbourhood composition and drought on th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24299-x
更新日期:2018-04-12 00:00:00
abstract::Increasing structural complexity at nanoscale can permit superior control over photophysical properties in the precursor-derived semiconductors. We demonstrate here the synthesis of silicon carbonitride (SiCN)/boron nitride (BN) nanocomposites via a polymer precursor route wherein the cobalt polyamine complexes used a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39866
更新日期:2017-01-13 00:00:00
abstract::The sinusoidal endothelial cells present in the liver (LSECs) are tipically characterized by the presence of pores (fenestrae). During some pathological conditions LSECs undergo "capillarization", a process characterized by loss of fenestrations and acquisition of a vascular phenotype. In chronic liver disease capilla...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-019-45114-1
更新日期:2019-06-19 00:00:00
abstract::We propose a scheme for generating an entangled state for two atoms trapped in two separate cavities coupled to each other. The scheme is based on the competition between the unitary dynamics induced by the classical fields and the collective decays induced by the dissipation of two non-local bosonic modes. In this sc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17933-7
更新日期:2017-12-15 00:00:00
abstract::Biomolecules for OMIC analysis of microbial communities are commonly extracted by bead-beating or ultra-sonication, but both showed varying yields. In addition to that, different disruption pressures are necessary to lyse bacteria and fungi. However, the disruption efficiency and yields comparing bead-beating and ultr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42188-9
更新日期:2019-04-04 00:00:00
abstract::Angiogenesis is essential in tumor biology and is regulated by vascular endothelial growth factor (VEGF) ligands and receptors. Here we aimed to discover genetic variants associated with levels of circulating angiogenic proteins in cancer patients. Plasma was collected at baseline in 216 pancreatic and 114 colorectal ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34506-4
更新日期:2018-11-05 00:00:00
abstract::Upwelling off Vietnam in the South China Sea (SCS) and the Sumatra-Java upwelling in the Indian Ocean significantly modulate regional variation in climate. Although located in different hemispheres, these upwellings nearly concur during the boreal summer; both are the result of wind-induced Ekman divergence. Beyond se...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40246-w
更新日期:2019-03-06 00:00:00
abstract::In contrast to mammals, adult fish display a remarkable ability to fully regenerate central nervous system (CNS) axons, enabling functional recovery from CNS injury. Both fish and mammals normally undergo a developmental downregulation of axon growth activity as neurons mature. Fish are able to undergo damage-induced ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50485-6
更新日期:2019-10-02 00:00:00
abstract::The bacterial second messenger cyclic di-3',5'-guanosine monophosphate (c-di-GMP) is a key regulator of bacterial motility and virulence. As high levels of c-di-GMP are associated with the biofilm lifestyle, c-di-GMP hydrolysing phosphodiesterases (PDEs) have been identified as key targets to aid development of novel ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42166
更新日期:2017-02-10 00:00:00
abstract::A novel sesquiterpene methylcyclopentenedione, penicilliumin B (1), was obtained from a deep sea-derived fungus Penicillium sp. F00120, together with three known sesquiterpene cyclohexenones (2-4). Penicilliumin B (1), presenting the first example with the sesquiterpene cyclopentenedione skeleton as natural products, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11007-4
更新日期:2017-09-07 00:00:00
abstract::Virus production in the deep-sea environment has been found to be high, and viruses have been suggested to play significant roles in the overall functioning of this ecosystem. Nevertheless, little is known about these viruses, including the mechanisms that control their production, which makes them one of the least un...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21908
更新日期:2016-02-22 00:00:00
abstract::Vegetation buffers local diurnal land surface temperatures, however, this effect has found limited applications for remote vegetation characterization. In this work, we parameterize diurnal temperature variations as the thermal decay rate derived by using satellite daytime and nighttime land surface temperatures and m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66193-5
更新日期:2020-06-17 00:00:00
abstract::Myopic traction maculopathy (MTM) can cause vision disabilities in highly myopic eyes. This retrospective case series investigated the clinical outcomes of posterior scleral contraction (PSC) using genipin-cross-linked sclera as the material to treat MTM in highly myopic eyes. In total, 32 eyes from 29 highly myopic p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43256
更新日期:2017-02-21 00:00:00
abstract::Oviposition site-selection in insects is mediated through innate recognition templates (IRTs) tuned to specific chemical cues. These cues aid gravid insects in choosing suitable oviposition sites and may even enhance the fitness of their offspring by warding off predators and parasitoids. However, studies on the evolu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07472
更新日期:2014-12-15 00:00:00
abstract::Maximal rate of heart rate (HR) increase (rHRI) as a measure of HR acceleration during the transition from rest to exercise, or during an increase in workload, tracks exercise performance. rHRI assessed at relative rather than absolute workloads may track performance better, and a field test would increase applicabili...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71597-4
更新日期:2020-09-03 00:00:00
abstract::Gayal (Bos frontalis) is a semi-wild and endangered bovine species that differs from domestic cattle (Bos taurus and Bos indicus), and its genetic background remains unclear. Here, we performed whole-genome sequencing of one Gayal for the first time, with one Red Angus cattle and one Japanese Black cattle as controls....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19787
更新日期:2016-01-25 00:00:00
abstract::Based on a hybrid pumping method consisting of a 1150 nm continuous-wave pump source and a 1950 nm pulsed pump source, we demonstrate a power controllable gain-switched fiber laser in dual wavebands at ~ 3 μm and ~ 2.1 μm. Different pumping schemes for pumping a Ho3+-doped ZBLAN fiber are studied. Using only the 1950 ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80238-9
更新日期:2021-01-13 00:00:00
abstract::An abrupt onset of the new tongue-shaped deformation of magnetic surface in magnetized plasmas, which was conjectured in since the 1960s but has not been observed, is experimentally identified just before an abrupt onset of a large-scale collapse event. Two novel properties of the event are identified. First, the tran...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36217
更新日期:2016-10-31 00:00:00
abstract::Pseudooxynicotine amine oxidase (Pnao) is essential to the pyrrolidine pathway of nicotine degradation of Pseudomonas putida strain S16, which is significant for the detoxification of nicotine, through removing the CH3NH2 group. However, little is known about biochemical mechanism of this enzyme. Here, we characterize...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17770
更新日期:2015-12-04 00:00:00
abstract::Influence maximization is the problem of finding the set of nodes of a network that maximizes the size of the outbreak of a spreading process occurring on the network. Solutions to this problem are important for strategic decisions in marketing and political campaigns. The typical setting consists in the identificatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51209-6
更新日期:2019-10-22 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is one of the most common respiratory diseases. Yihuo Huatan Formula (YHF), as a proven Chinese Herbal Medicine (CHM), has been verified to be effective in the treatment of stable COPD through years' of practice. Nevertheless, its working mechanism is still unclear. We soug...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50064-9
更新日期:2019-09-17 00:00:00
abstract::De novo uridine-diphosphate-N-acetylglucosamine (UDP-GlcNAc) biosynthesis requires glucose, glutamine, acetyl-CoA and uridine, however GlcNAc salvaged from glycoconjugate turnover and dietary sources also makes a significant contribution to the intracellular pool. Herein we ask whether dietary GlcNAc regulates nutrien...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23043
更新日期:2016-03-14 00:00:00
abstract::Natural medicines (i.e., herbal medicines, traditional formulas) are useful for treatment of multifactorial and chronic diseases. Here, we present KampoDB ( http://wakanmoview.inm.u-toyama.ac.jp/kampo/ ), a novel platform for the analysis of natural medicines, which provides various useful scientific resources on Japa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29516-1
更新日期:2018-07-25 00:00:00
abstract::The pace of two-dimensional materials (2DM) research has been greatly accelerated by the ability to identify exfoliated thicknesses down to a monolayer from their optical contrast. Since this process requires time-consuming and error-prone manual assignment to avoid false-positives from image features with similar con...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23922-1
更新日期:2018-04-23 00:00:00
abstract::The hitherto difficult task of reliably estimating populations of wide-ranging megafauna has been enabled by advances in capture-recapture methodology. Here we combine photographic sampling with a Bayesian spatially-explicit capture-recapture (SCR) model to estimate population parameters for the endangered Asian eleph...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44795-y
更新日期:2019-06-12 00:00:00
abstract::Measurements of skeletal muscle cross-sectional area, index, and radiation attenuation utilizing clinical computed tomography (CT) scans are used in assessments of sarcopenia, the loss of skeletal muscle mass and function associated with aging. To classify individuals as sarcopenic, sex-specific cutoffs for 'low' valu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29825-5
更新日期:2018-07-27 00:00:00
abstract::We study the dynamical Casimir-Polder force on a two-level atom with different initial states in the one-dimensional dielectric cavity with output coupling, and obtain the analytical expression of the expectation value of dynamical Casimir-Polder force. Results show that the expectation values of dynamical Casimir-Pol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68546-6
更新日期:2020-07-20 00:00:00
abstract::Elucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77846-w
更新日期:2020-12-18 00:00:00