Abstract：
:The 28,000-year-old remains of a woolly mammoth, named 'Yuka', were found in Siberian permafrost. Here we recovered the less-damaged nucleus-like structures from the remains and visualised their dynamics in living mouse oocytes after nuclear transfer. Proteomic analyses demonstrated the presence of nuclear components in the remains. Nucleus-like structures found in the tissue homogenate were histone- and lamin-positive by immunostaining. In the reconstructed oocytes, the mammoth nuclei showed the spindle assembly, histone incorporation and partial nuclear formation; however, the full activation of nuclei for cleavage was not confirmed. DNA damage levels, which varied among the nuclei, were comparable to those of frozen-thawed mouse sperm and were reduced in some reconstructed oocytes. Our work provides a platform to evaluate the biological activities of nuclei in extinct animal species.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Yamagata K,Nagai K,Miyamoto H,Anzai M,Kato H,Miyamoto K,Kurosaka S,Azuma R,Kolodeznikov II,Protopopov AV,Plotnikov VV,Kobayashi H,Kawahara-Miki R,Kono T,Uchida M,Shibata Y,Handa T,Kimura H,Hosoi Y,Mitani T,Matsumodoi
10.1038/s41598-019-40546-1subject
Has Abstractpub_date
2019-03-11 00:00:00pages
4050issue
1issn
2045-2322pii
10.1038/s41598-019-40546-1journal_volume
9pub_type
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