Abstract：
:Walnuts (Juglans spp.) are economically important nut and timber species with a worldwide distribution. Using the published Persian walnut genome as a reference for the assembly of short reads from six Juglans species and several interspecific hybrids, we identified simple sequence repeats in 12 Juglans nuclear and organellar genomes. The genome-wide distribution and polymorphisms of nuclear and organellar microsatellites (SSRs) for most Juglans genomes have not been previously studied. We compared the frequency of nuclear SSR motifs and their lengths across Juglans, and identified section-specific chloroplast SSR motifs. Primer pairs were designed for more than 60,000 SSR-containing sequences based on alignment against assembled scaffold sequences. Of the >60,000 loci, 39,000 were validated by e-PCR using unique primer pairs. We identified primers containing 100% sequence identity in multiple species. Across species, sequence identity in the SSR-flanking regions was generally low. Although SSRs are common and highly dispersed in the genome, their flanking sequences are conserved at about 90 to 95% identity within Juglans and within species. In a few rare cases, flanking sequences are identical across species of Juglans. This comprehensive report of nuclear and organellar SSRs in Juglans and the generation of validated SSR primers will be a useful resource for future genetic analyses, walnut breeding programs, high-level taxonomic evaluations, and genomic studies in Juglandaceae.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ebrahimi A,Mathur S,Lawson SS,LaBonte NR,Lorch A,Coggeshall MV,Woeste KEdoi
10.1038/s41598-019-39793-zsubject
Has Abstractpub_date
2019-03-06 00:00:00pages
3748issue
1issn
2045-2322pii
10.1038/s41598-019-39793-zjournal_volume
9pub_type
杂志文章abstract：:The factors regulating phytoplankton community composition play a crucial role in structuring aquatic food webs. However, consensus is still lacking about the mechanisms underlying the observed biogeographical differences in cell size composition of phytoplankton communities. Here we use a trait-based model to disenta...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08918
更新日期：2015-03-09 00:00:00
abstract：:Sonic hedgehog (Shh) is a multifunctional signaling protein governing pattern formation, proliferation and cell survival during embryogenesis. In the adult brain, Shh has neurotrophic function and is implicated in hippocampal neurogenesis but the cellular source of Shh in the hippocampus remains ill defined. Here, we ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53192-4
更新日期：2019-11-22 00:00:00
abstract：:Some 1,3-diarylureas and 1-((1,4-trans)-4-aryloxycyclohexyl)-3-arylureas (cHAUs) activate heme-regulated kinase causing protein synthesis inhibition via phosphorylation of the eukaryotic translation initiation factor 2 (eIF2) in mammalian cancer cells. To evaluate if these agents have potential to inhibit trypanosome ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23259-9
更新日期：2018-03-20 00:00:00
abstract：:Corals access inorganic seawater nutrients through their autotrophic endosymbiotic dinoflagellates, but also capture planktonic prey through heterotrophic feeding. Correlating NanoSIMS and TEM imaging, we visualized and quantified the subcellular fate of autotrophic and heterotrophic C and N in the coral Stylophora pi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31094-1
更新日期：2018-08-23 00:00:00
abstract：:Simulation models in respiratory research are increasingly used for medical product development and testing, especially because in-vivo models are coupled with a high degree of complexity and ethical concerns. This work introduces a respiratory simulation system, which is bridging the gap between the complex, real ana...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56176-6
更新日期：2019-12-24 00:00:00
abstract：:Bacteriophage capsids constitute icosahedral shells of exceptional stability that protect the viral genome. Many capsids display on their surface decoration proteins whose structure and function remain largely unknown. The decoration protein pb10 of phage T5 binds at the centre of the 120 hexamers formed by the major ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41662
更新日期：2017-02-06 00:00:00
abstract：:Green fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence. This coverage dip was present when virus-derived R...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26314
更新日期：2016-05-19 00:00:00
abstract：:Malaria transmission-blocking vaccines (TBVs) target the development of Plasmodium parasites within the mosquito, with the aim of preventing malaria transmission from one infected individual to another. Different vaccine platforms, mainly protein-in-adjuvant formulations delivering the leading candidate antigens, have...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11193
更新日期：2015-06-11 00:00:00
abstract：:Numerical aberration of the centrosome results in chromosome missegregation, eventually leading to chromosomal instability, a hallmark of human tumor malignancy. Large tumor suppressors 1 and 2 (Lats1 and Lats2) are central kinases in the Hippo pathway and regulate development and tumorigenesis by coordinating the bal...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16173
更新日期：2015-11-04 00:00:00
abstract：:Poor oral health is not only associated with diabetes and cardiovascular disease but adverse pregnancy outcomes. However the influence of dental caries on pregnancy is unknown. The aim of this study was to evaluate the association between dental caries and adverse pregnancy outcomes and the effect of treatment for den...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62306-2
更新日期：2020-03-24 00:00:00
abstract：:Successful recruitment of new individuals is essential for recovery of degraded coral reefs. Enhancing supply of coral larvae increases initial settlement, however post-settlement survival can be influenced by density-dependent processes. We investigated the influence of larval density on settlement, colony abundance ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62366-4
更新日期：2020-03-26 00:00:00
abstract：:Here we present a set of measurements using Differential Scanning Fluorimetry (DSF) as an inexpensive, high throughput screening method to investigate the folding of silk protein molecules as they abandon their first native melt conformation, dehydrate and denature into their final solid filament conformation. Our fir...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05625
更新日期：2014-07-09 00:00:00
abstract：:Silver nanoparticles (AgNPs) have been extensively used and are considered as an emerging contaminant in the ocean. The environmental contamination of AgNPs is expected to increase greatly over time, and cetaceans, as the top ocean predators, will suffer the negative impacts of AgNPs. In the present study, we investig...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23737-0
更新日期：2018-04-04 00:00:00
abstract：:Learning rules by which cell shape impacts cell function would enable control of cell physiology and fate in medical applications, particularly, on the interface of cells and material of the implants. We defined the phenotypic response of human bone marrow-derived mesenchymal stem cells (hMSCs) to 2176 randomly genera...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76019-z
更新日期：2020-11-04 00:00:00
abstract：:Silicon nanowires (SiNWs) attached to a wafer substrate are converted to inversely tapered silicon nanocones (SiNCs). After excitation with visible light, individual SiNCs show a 200-fold enhanced integral band-to-band luminescence as compared to a straight SiNW reference. Furthermore, the reverse taper is responsible...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17089
更新日期：2015-11-26 00:00:00
abstract：:Indoor microbial communities play an important role in everyday human health, especially in the intensive care units (ICUs) of hospitals. We used amplicon pyrosequencing to study the ICU microbiome and were able to detect diverse sequences, in comparison to the currently used standard cultivation technique that only d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01413
更新日期：2013-01-01 00:00:00
abstract：:The application of machine learning to predict materials' properties usually requires a large number of consistent data for training. However, experimental datasets of high quality are not always available or self-consistent. Here, as an alternative route, we combine machine learning with high-throughput molecular dyn...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45344-3
更新日期：2019-06-19 00:00:00
abstract：:Chromosomal microarray (CMA) is now recommended as first tier for the evaluation in individuals with unexplained neurodevelopmental disorders (ND). However, in developing countries such as Brazil, classical cytogenetic tests are still the most used in clinical practice, as reflected by the scarcity of publications of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54347-z
更新日期：2019-11-28 00:00:00
abstract：:Although aversive memory has been mainly addressed by analysing the changes occurring in average populations, the study of neuronal mechanisms of outliers allows understanding the involvement of individual differences in fear conditioning and extinction. We recently developed an innovative experimental model of indivi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21205
更新日期：2016-02-15 00:00:00
abstract：:Application of PCR to multiplexing assays is not trivial; it requires multiple fluorescent labels for amplicon detection and sophisticated software for data interpretation. Alternative PCR-free methods exploiting new concepts in nanotechnology exhibit high sensitivities but require multiple labeling and/or amplificati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02033
更新日期：2013-01-01 00:00:00
abstract：:It is well known the thermal properties of three-dimensional (3-D) hybrid graphene (GR)-carbon nanotube (CNT) structures are not superior to that of the individual GR and CNT, however, the 3-D hybrid GR-CNT structures can effectively improve the thermal properties of polymer matrix. Therefore, understanding the therma...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14710-4
更新日期：2017-10-31 00:00:00
abstract：:While researches have focused on drag reduction of various coated surfaces such as superhydrophobic structures and polymer brushes, the insights tso understand the fundamental physics of the laminar skin friction coefficient and the related drag reduction due to the formation of finite velocity at porous surfaces is s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17141-3
更新日期：2017-12-08 00:00:00
abstract：:The prime function of nucleoli is ribogenesis, however, several other, non-canonical functions have recently been identified, including a role in genotoxic stress response. Upon DNA damage, numerous proteins shuttle dynamically between the nucleolus and the nucleoplasm, yet the underlying molecular mechanisms are inco...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46358-7
更新日期：2019-07-11 00:00:00
abstract：:Field electron emission (FEE) properties of vertically aligned hexagonal boron nitride nanowalls (hBNNWs) grown on Si have been markedly enhanced through the use of nitrogen doped nanocrystalline diamond (nNCD) films as an interlayer. The FEE properties of hBNNWs-nNCD heterostructures show a low turn-on field of 15.2 ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29444
更新日期：2016-07-11 00:00:00
abstract：:Caterpillars of the silk moth genus Hyalophora (Lepidoptera; Saturniidae) construct multilayered cocoons that function as overwintering housing during the pupal to adult developmental period. While all cocoons share the primary function of protecting the developing moth, cocoons spun by different Hyalophora silk moth ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62547-1
更新日期：2020-03-27 00:00:00
abstract：:We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18034-1
更新日期：2017-12-14 00:00:00
abstract：:The spectral distribution of light emitted from a scanning tunnelling microscope junction not only bears its intrinsic plasmonic signature but is also imprinted with the characteristics of optical frequency fluc- tuations of the tunnel current. Experimental spectra from gold-gold tunnel junctions are presented that sh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03766-x
更新日期：2017-06-14 00:00:00
abstract：:Complex systems performing spiking dynamics are widespread in Nature. They cover from earthquakes, to neurons, variable stars, social networks, or stock markets. Understanding and characterizing their dynamics is relevant in order to detect transitions, or to predict unwanted extreme events. Here we study, under an or...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29110-5
更新日期：2018-07-16 00:00:00
abstract：:One procedure widely used to detect the velocity of a moving object is by using the Doppler effect. This is the perceived change in frequency of a wave caused by the relative motion between the emitter and the detector, or between the detector and a reflecting target. The relative movement, in turn, generates a time-v...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02815
更新日期：2013-10-02 00:00:00
abstract：:Glucocorticoid receptor is a transcription factor that is ubiquitously expressed. Glucocorticoids are circadian steroids that regulate a wide range of bodily functions, including immunity. Here we report that synthetic glucocorticoids affect 1035 mRNAs in isolated healthy human blood monocytes but only 165 in the resp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39395-9
更新日期：2019-02-26 00:00:00
