Abstract:
:Superresolution microscopy offers the advantage of imaging biological structures within cells at the nano-scale. Here we apply two superresolution microscopy techniques, specifically 3D structured illumination microscopy (3D-SIM) and direct stochastic optical reconstruction microscopy (dSTORM), a type of single molecule localisation microscopy, to localise IRSp53 protein and its I-BAR domain in relation to F-actin within filopodia. IRSp53 generates dynamic (extending and retracting) filopodia 300 nm wide with a distinct gap between IRSp53 and F-actin. By contrast, protrusions induced by the I-BAR domain alone are non-dynamic measuring between 100-200 nm in width and exhibit a comparatively closer localisation of the I-BAR domain with the F-actin. The data suggest that IRSp53 membrane localisation is spatially segregated to the lateral edges of filopodia, in contrast to the I-BAR domain is uniformly distributed throughout the membranes of protrusions. Modeling of fluorescence recovery after photobleaching (FRAP) data suggests that a greater proportion of I-BAR domain is associated with membranes when compared to full length IRSp53. The significance of this new data relates to the role filopodia play in cell migration and its importance to cancer.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sudhaharan T,Hariharan S,Lim JSY,Liu JZ,Koon YL,Wright GD,Chiam KH,Ahmed Sdoi
10.1038/s41598-019-38851-wsubject
Has Abstractpub_date
2019-02-21 00:00:00pages
2524issue
1issn
2045-2322pii
10.1038/s41598-019-38851-wjournal_volume
9pub_type
杂志文章abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-65654-1
更新日期:2020-05-26 00:00:00
abstract::Ovarian cancer is the second most common gynaecologic malignancy and the main cause of death from gynaecologic cancer, due to late diagnosis and chemoresistance. Studies have reported the role of cysteine in cancer, by contributing for hydrogen sulphide (H2S) generation and as a precursor of glutathione (GSH). However...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27753-y
更新日期:2018-06-22 00:00:00
abstract::Organic-inorganic halide perovskite nanocrystals or quantum dots (PQDs) are excellent candidates for optoelectronic applications, such as lasers, solar cells, light emitting diodes, and single photon sources. However, the potential applications of PQDs can expand once the photoluminescence, and in particular, the blin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58926-3
更新日期:2020-02-07 00:00:00
abstract::Elymus nutans is an herbaceous plant that can be used to restore degraded alpine and subalpine ecosystems. Here, we evaluated how sowing density affects soil reinforcement and slope stabilization properties of vegetation-concrete structures. To investigate the optimal sowing density of E. nutans in vegetation-concrete...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77407-1
更新日期:2020-11-24 00:00:00
abstract::An in vitro-transcribed RNA aptamer (trans-RA16) that targets non-small cell lung cancer (NSCLC) was previously identified through in vivo SELEX. Trans-RA16 can specifically target and inhibit human NCI-H460 cells in vitro and xenograft tumors in vivo. Here, in a follow-up study, we obtained a chemically-synthesized v...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55280-x
更新日期:2019-12-11 00:00:00
abstract::The 37 currently recognized Bemisia tabaci cryptic species are economically important species and contain both primary and secondary endosymbionts, but their diversity has never been mapped systematically across the group. To achieve this, PacBio sequencing of full-length bacterial 16S rRNA gene amplicons was carried ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42793-8
更新日期:2019-04-25 00:00:00
abstract::Prostate-specific antigen (PSA) kinetics predicts survival in castration-resistant prostate cancer (CRPC); however, the influence of prior treatment on this relationship is unclear. Patients with CRPC were stratified according to time to PSA nadir and time to CRPC progression to investigate their prognostic significan...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48424-6
更新日期:2019-08-15 00:00:00
abstract::The ammonia removal as well as the current efficiency during electrolysis was investigated by using a vermiculite-packed electrochemical reactor under continuous mode. Experimental results showed that adsorption of ammonia by vermiculite and electrolytic desorption of ammonia simultaneously existed in the reactor, lea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41030
更新日期:2017-01-19 00:00:00
abstract::This study investigates the relationship between objective cognitive impairment (OCI), subjective cognitive complaints and depressive symptoms in men and women with classical and non-classical Fabry disease (FD). Cognitive functioning was assessed using a neuropsychological test battery, subjective cognitive complaint...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37320-0
更新日期:2019-01-17 00:00:00
abstract::Super-Planckian near-field radiative heat transfer allows effective heat transfer between a hot and a cold body to increase beyond the limits long known for black bodies. Until present, experimental techniques to measure the radiative heat flow relied on steady-state systems. Here, we present a dynamic measurement app...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14242-x
更新日期:2017-10-24 00:00:00
abstract::Copper ions play a vital role in a variety of fundamental physiological processes not only in human beings and plants, but also for extensive insects and microorganisms. In this paper, a novel water-soluble ruthenium(II) complex as a turn-on copper(II) ions luminescent sensor based on o-(phenylazo)aniline was designed...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08172
更新日期:2015-02-02 00:00:00
abstract::Ferritin has gained significant attention as a potential reporter gene for in vivo imaging by magnetic resonance imaging (MRI). However, due to the ferritin ferrihydrite core, the relaxivity and sensitivity for detection of native ferritin is relatively low. We report here on a novel chimeric magneto-ferritin reporter...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26550
更新日期:2016-05-23 00:00:00
abstract::Ruminants obtain nutrients from microbial fermentation of plant material, primarily in their rumen, a multilayered forestomach. How the different layers of the rumen wall respond to diet and influence microbial fermentation, and how these process are regulated, is not well understood. Gene expression correlation netwo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39022
更新日期:2016-12-14 00:00:00
abstract::Complex systems, arising in many contexts in the computer, life, social, and physical sciences, have not shared a generally-accepted complexity measure playing a fundamental role as the Shannon entropy H in statistical mechanics. Superficially-conflicting criteria of complexity measurement, i.e. complexity-randomness ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01585
更新日期:2013-01-01 00:00:00
abstract::Psoriasis is a chronic, relapsing inflammatory skin disease. The pathogenesis of psoriasis is complex and has not been fully understood. C10orf99 was a recently identified human antimicrobial peptide whose mRNA expression is elevated in psoriatic human skin samples. In this study, we investigated the functional roles ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26996-z
更新日期:2018-06-05 00:00:00
abstract::Electrochemically active bacteria (EAB) are ubiquitous in environment and have important application in the fields of biogeochemistry, environment, microbiology and bioenergy. However, rapid and sensitive methods for EAB identification and evaluation of their extracellular electron transfer ability are still lacking. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01315
更新日期:2013-01-01 00:00:00
abstract::TLR4 location, and bacterial species-derived lipopolysaccharides, play a significant role in the downstream activation of transcription factors, accessory molecules, and products. Here, this is demonstrated through the use of classically-activated and alternatively-activated macrophages. We show that, when polarized, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79145-w
更新日期:2021-01-14 00:00:00
abstract::The rapid rise of bacterial resistance to traditional antibiotics combined with the decline in discovery of novel antibacterial agents has created a global public health crisis. Repurposing existing drugs presents an alternative strategy to potentially expedite the discovery of new antimicrobial drugs. The present stu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16407
更新日期:2015-11-10 00:00:00
abstract::The anhydrofructose pathway is an alternate pathway for glycogen degradation by α-1,4-glucan lyase. The sugar 1,5-anhydro-D-fructose (1,5-AF) acts as the central intermediate of this pathway, but its physiological role of in mammals is unclear. Glycation reactions forming advanced glycation end-products (AGEs) are imp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46333-2
更新日期:2019-07-15 00:00:00
abstract::Overexposure to intense noise can destroy the synapses between auditory nerve fibers and their hair cell targets without destroying the hair cells themselves. In adult mice, this synaptopathy is immediate and largely irreversible, whereas, in guinea pigs, counts of immunostained synaptic puncta can recover with increa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76553-w
更新日期:2020-11-17 00:00:00
abstract::The product of the meiosis-expressed gene 1 (MEIG1) is found in the cell bodies of spermatocytes and recruited to the manchette, a structure unique to elongating spermatids, by Parkin co-regulated gene (PACRG). This complex is essential for targeting cargo to the manchette during sperm flagellum assembly. Here we show...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18278
更新日期:2016-01-04 00:00:00
abstract::Human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are the major etiological agents of hand, foot and mouth disease (HFMD) and are often associated with neurological complications. Currently, several vaccine types are being developed for EV71 and CA16. In this study, we constructed a bivalent chimeric virus-lik...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12973
更新日期:2015-08-05 00:00:00
abstract::The present work focuses on the postseismic deformation observed in the region of L'Aquila (central Italy) following the Mw 6.3 earthquake that occurred on April 6, 2009. A new, 16-month-long dataset of COSMO-SkyMed SAR images was analysed using the Persistent Scatterer Pairs interferometric technique. The analysis re...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16558
更新日期:2015-11-10 00:00:00
abstract::The recent combination of ultra-intense lasers and laser-accelerated electron beams is enabling the development of a new generation of compact x-ray light sources, the coherence of which depends directly on electron beam emittance. Although the emittance of accelerated electron beams can be low, it can grow due to the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24622
更新日期:2016-04-19 00:00:00
abstract::Shewanella species are facultative anaerobic bacteria that colonize redox-stratified habitats where O2 and nutrient concentrations fluctuate. The model species Shewanella oneidensis MR-1 possesses genes coding for three terminal oxidases that can perform O2 respiration: a bd-type quinol oxidase and cytochrome c oxidas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19726
更新日期:2016-01-27 00:00:00
abstract::While many informal factors influence how people interact, modern societies rely upon law as a primary mechanism to formally control human behaviour. How legal rules impact societal development depends on the interplay between two types of actors: the people who create the rules and the people to which the rules poten...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73623-x
更新日期:2020-10-30 00:00:00
abstract::Lower cervical dislocation with facet interlocking is one of the most drastic injuries to the cervical spine. The early reduction is thought critical in preventing progressive secondary spinal cord injury. The authors report a new surgical procedure in the management of lower cervical dislocation with facet interlocki...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37742-w
更新日期:2019-02-04 00:00:00
abstract::SIRT1 is one of seven mammalian homologs of Sir2 that catalyzes NAD(+)-dependent protein deacetylation. The aim of the present study is to explore the effect of SIRT1 small molecule activator on the anticancer activity and the underlying mechanism. We examined the anticancer activity of a novel oral agent, curcumin, w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13429
更新日期:2015-08-24 00:00:00
abstract::Wholesale, unbiased assessment of Scandinavian electronic health-care databases offer a unique opportunity to reveal potentially important undiscovered drug side effects. We examined the short-term risk of acute myocardial infarction (AMI) associated with drugs prescribed in Norway or Sweden. We identified 24,584 and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44641-1
更新日期:2019-06-04 00:00:00
abstract::The transcriptional responses of yeast cells to diverse stresses typically include gene activation and repression. Specific stress defense, citric acid cycle and oxidative phosphorylation genes are activated, whereas protein synthesis genes are coordinately repressed. This view was achieved from comparative transcript...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26170-5
更新日期:2018-05-21 00:00:00