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A monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for flubendiamide detection.

Abstract：

:Flubendiamide (FD), the first commercial phthalic acid diamide that targets insect ryanodine receptor (RyRs), has played an important role in pest management. With its extensive worldwide application, a rapid and convenient method to detect its existence in the environment is necessary. In this study, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to analyse FD residue on environmental and food samples. The established icELISA showed a half maximal inhibition concentration (IC50) of 17.25 µg L-1, with a working range of 4.06-103.59 µg L-1 for FD, and showed no cross-reactivity with chlorantraniliprole, cyantraniliprole, and several FD analogues. Average FD recoveries from spinach, tap water, and soil samples were 89.3-112.3%, 93.0-102.1%, and 86.9-97.6%, respectively. Meanwhile, FD detection results of icELISA were compared with those of ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The comparable results verified that icELISA was suitable for rapid detection of FD residue in environmental and agricultural samples.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Li Q,Cui Y,Liao M,Feng T,Tan G,Wang B,Liu S

doi

10.1038/s41598-019-38649-w

subject

Has Abstract

pub_date

2019-02-14 00:00:00

pages

2131

issue

1

issn

2045-2322

pii

10.1038/s41598-019-38649-w

journal_volume

9

pub_type

杂志文章

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