Abstract：
:Although it is known that malaria parasites proliferate in the midgut of mosquito vector, their detailed behaviors, from gamete maturation to formation of next generation sporozoite, have not been fully understood at cellular or molecular level. This is mainly attributed to technical difficulties of dissection and whole-mount observation, of delicate and opaque mosquito body contents. In addition, blood pigment surrounding parasites immediately after blood meal also complicates tracing mosquito-stage parasites. Recent revolutionary studies have overcome such negative factors in tissue observation by clearing organisms. CUBIC reagents succeeded to remove both light scattering and blood pigment from various mouse tissues, and to whole-organ image fluorescence-labeled cell structures. In this study, we utilized the advanced version of CUBIC technology and high sensitivity fluorescent markers for see-through observation of mosquito vector after engulfment of rodent malaria parasites to clarify their behaviors during mosquito stage. As a result, we succeeded to visualize oocysts, sporozoites, female gametes and ookinetes in the mosquito bodies without any dissection.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Mori T,Hirai M,Mita Tdoi
10.1038/s41598-019-38529-3subject
Has Abstractpub_date
2019-02-11 00:00:00pages
1768issue
1issn
2045-2322pii
10.1038/s41598-019-38529-3journal_volume
9pub_type
杂志文章abstract：:Recently, quantum Hall state analogs in classical mechanics attract much attention from topological points of view. Topology is not only for mathematicians but also quite useful in a quantum world. Further it even governs the Newton's law of motion. One of the advantages of classical systems over solid state materials...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18107
更新日期：2015-12-15 00:00:00
abstract：:The South American Monsoon System (SAMS) is generally considered to be highly sensitive to Northern Hemisphere (NH) temperature variations on multi-centennial timescales. The direct influence of solar forcing on moisture convergence in global monsoon systems on the other hand, while well explored in modeling studies, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24762
更新日期：2016-04-21 00:00:00
abstract：:Although the growth of graphene by chemical vapor deposition is a production technique that guarantees high crystallinity and superior electronic properties on large areas, it is still a challenge for manufacturers to efficiently scale up the production to the industrial scale. In this context, issues related to the p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09811-z
更新日期：2017-08-30 00:00:00
abstract：:The objective of our in vivo study was to compare the effects of the osteotomy on the thermal alterations, the bone healing and count of polymorphonuclear cells, comparing the drill design (cylindrical or conical) using continuous or intermittent movement. Twelve rabbits were used, which were made four osteotomies (n ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77762-z
更新日期：2020-11-26 00:00:00
abstract：:Sheepgrass (Leymus chinensis) is one of the dominant grass species present on typical steppes of the Inner Mongolia Plateau. However, L. chinensis has developed a dwarfing phenotype in response to the stressful habitat in grasslands that are severely degraded due to heavy grazing. The lack of transcriptomic and genomi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35605-y
更新日期：2018-12-17 00:00:00
abstract：:Controlled surface wettability for oil has been intensively studied to remove industrial oil waste or oil spill pollution from seas or rivers. In particular, external stimuli-induced special wetting materials, such as photo-responsive TiO2, have attracted considerable attention for oil-water separation. In this study,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12908
更新日期：2015-08-11 00:00:00
abstract：:Human KHDRBS1/Sam68 is an oncogenic splicing factor involved in signal transduction and pre-mRNA splicing. We explored the molecular mechanism of KHDRBS1 to be a prognostic marker in four different cancers. Within specific cancer, including kidney renal papillary cell carcinoma (KIRP), lung adenocarcinoma (LUAD), acut...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47558-x
更新日期：2019-07-31 00:00:00
abstract：:Diabetes is a globally prevalent disease that can cause visible microvascular complications such as diabetic retinopathy and macular edema in the human eye retina, the images of which are today used for manual disease screening and diagnosis. This labor-intensive task could greatly benefit from automatic detection usi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47181-w
更新日期：2019-07-24 00:00:00
abstract：:Polyphenol oxidases (PPOs) comprise tyrosinases (TYRs) and catechol oxidases (COs), which catalyse the initial reactions in the biosynthesis of melanin. TYRs hydroxylate monophenolic (monophenolase activity) and oxidize diphenolic (diphenolase activity) substrates, whereas COs react only with diphenols. In order to el...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57671-x
更新日期：2020-02-03 00:00:00
abstract：:Cinnabar is a natural mercury sulfide (HgS) mineral of volcanic or hydrothermal origin that is found worldwide. It has been mined prehistorically and historically in China, Japan, Europe, and the Americas to extract metallic mercury (Hg(0)) for use in metallurgy, as a medicinal, a preservative, and as a red pigment fo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14679
更新日期：2015-10-01 00:00:00
abstract：:Cardiac progenitor cells (CPCs) have been shown to promote cardiac regeneration and improve heart function. However, evidence suggests that their regenerative capacity may be limited in conditions of severe hypoxia. Elucidating the mechanisms involved in CPC protection against hypoxic stress is essential to maximize t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09813-x
更新日期：2017-08-29 00:00:00
abstract：:A two step excitation scheme in hot atomic sodium vapor is experimentally investigated. The observed effects reflect a coupling between the 32S, 32P and the 32D states. We present the relative dependence on detuning of the two utilized lasers around λ = 589 nm and 819 nm. Unlike expected, we achieve a higher detuning ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12089-w
更新日期：2017-09-18 00:00:00
abstract：:Rotator cuff tears are common and are associated with shoulder pain, disability, and dysfunction. Previous studies that have reported different arthroscopic techniques are controversial. A network meta-analysis with indirect and direct evidence was performed to compare the effectiveness of arthroscopic techniques for ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-019-40641-3
更新日期：2019-03-11 00:00:00
abstract：:Melanoma is the most lethal cutaneous cancer. New drugs have recently appeared; however, not all patients obtain a benefit of these new drugs. For this reason, it is still necessary to characterize melanoma at molecular level. The aim of this study was to explore the molecular differences between melanoma tumor subtyp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43512-z
更新日期：2019-05-10 00:00:00
abstract：:Results from epidemiological and prospective studies indicate a close association between periodontitis and diabetes. However the mechanisms by which periodontal pathogens influence the development of prediabetes/diabetes are not clear. We previously reported that oral administration of a periodontal pathogen, Porphyr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65828-x
更新日期：2020-06-19 00:00:00
abstract：:Sea surface temperature (SST) patterns can - as surface climate forcing - affect weather and climate at large distances. One example is El Niño-Southern Oscillation (ENSO) that causes climate anomalies around the globe via teleconnections. Although several studies identified and characterized these teleconnections, ou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45423-5
更新日期：2019-06-19 00:00:00
abstract：:The circadian clock coordinates an organism's growth, development and physiology with environmental factors. One illuminating example is the rhythmic growth of hypocotyls and cotyledons in Arabidopsis thaliana. Such daily oscillations in leaf position are often referred to as sleep movements or nyctinasty. Here, we re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65372-8
更新日期：2020-05-26 00:00:00
abstract：:Compared with the commercially available single nucleotide polymorphism (SNP) chip based on the Bead Chip technology, the solution hybrid selection (SHS)-based target enrichment SNP chip is not only design-flexible, but also cost-effective for genotype sequencing. In this study, we propose to design an animal SNP chip...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09285-z
更新日期：2017-08-17 00:00:00
abstract：:Staphylococcus aureus strains carrying enterotoxin A gene (sea) causes food poisoning and cannot be distinguished from non-pathogenic strains by the culture method. Here, we developed a rapid, specific and sensitive visual detection of sea using loop-mediated isothermal amplification (LAMP) combined with nanogold prob...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64710-0
更新日期：2020-05-08 00:00:00
abstract：:Estimates suggest that at least half of all extant insect genera harbor obligate bacterial mutualists. Whereas an endosymbiotic relationship imparts many benefits upon host and symbiont alike, the intracellular lifestyle has profound effects on the bacterial genome. The obligate endosymbiont genome is a product of opp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31796-6
更新日期：2018-09-07 00:00:00
abstract：:The preferential degeneration of Substantia nigra dopamine midbrain neurons (SN DA) causes the motor-symptoms of Parkinson's disease (PD). Voltage-gated L-type calcium channels (LTCCs), especially the Cav1.3-subtype, generate an activity-related oscillatory Ca(2+) burden in SN DA neurons, contributing to their degener...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13688
更新日期：2015-09-18 00:00:00
abstract：:Spreading rumors on the Internet has become increasingly pervasive due to the proliferation of online social media. This paper investigates how rumors are amplified by a group of users who share similar interests or views, dubbed as an echo chamber. To this end, we identify and analyze 'rumor' echo chambers, each of w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57272-3
更新日期：2020-01-15 00:00:00
abstract：:Acyl-CoA:diacylglycerol acyltransferase I (DGAT1) is a key enzyme in lipogenesis which is increased in metabolically active cells to meet nutrient requirements. DGAT1 has been recognized as an anti-obesity target; however, its role in the tumor microenvironment remains unclear. We postulated that, in prostate cancer (...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39537-z
更新日期：2019-02-28 00:00:00
abstract：:Lysine-specific demethylase 1 (LSD1) is a histone demethylase that participates in transcriptional repression or activation. Recent studies reported that LSD1 is involved in learning and memory. Although LSD1 phosphorylation by PKCα was implicated in circadian rhythmicity, the importance of LSD1 phosphorylation in lea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05239-7
更新日期：2017-07-07 00:00:00
abstract：:Atomistic molecular dynamics simulations were performed with 13 non-peptidic neurotransmitters (NTs) in three different membrane environments. The results provide compelling evidence that NTs are divided into membrane-binding and membrane-nonbinding molecules. NTs adhere to the postsynaptic membrane surface whenever t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19345
更新日期：2016-01-19 00:00:00
abstract：:The interaction of light with biological tissues has been considered for various therapeutic applications. Light-induced neurite growth has the potential to be a clinically useful technique for neuron repair. However, most previous studies used either a large illumination area to accelerate overall neurite growth or e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54687-w
更新日期：2019-12-03 00:00:00
abstract：:Marek's disease virus (MDV) is a highly cell-associated alphaherpesvirus that causes deadly lymphomas in chickens. While vaccination protects against clinical symptoms, MDV field strains can still circulate in vaccinated flocks and continuously evolve towards greater virulence. MDV vaccines do not provide sterilizing ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67951-1
更新日期：2020-07-02 00:00:00
abstract：:Recent studies have demonstrated functions of miR-145 in vascular smooth muscle cells (VSMCs) phenotypes and vascular diseases. In this study, we aim to determine whether CD40 is involved in miR-145 mediated switch of VSMC phenotypes. In cultured VSMCs, the effects of miR-145 and CD40 on TNF-α, TGF-β, and Homocysteine...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35302
更新日期：2016-10-12 00:00:00
abstract：:The present study shows the thorough investigations on optical properties and hydrodynamic diameters of glutathione (GSH) stabilized nanosilver clusters (AgNC) at different stages of synthesis and engineering for the optimized absolute quantum yield to generate fluorescent images of Dalton Lymphoma Ascites (DLA) tumou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34843-4
更新日期：2018-11-07 00:00:00
abstract：:Y14 (RBM8A) is an RNA recognition motif-containing protein that forms heterodimers with MAGOH and serves as a core factor of the RNA surveillance machinery for the exon junction complex (EJC). The role of the Y14 C-terminal serine/arginine (RS) repeat-containing region, which has been reported to undergo modifications...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18765-1
更新日期：2018-01-12 00:00:00
