Abstract：
:Very recently, we postulated that the incorporation of citral into nanostructured lipid carrier (NLC-Citral) improves solubility and delivery of the citral without toxic effects in vivo. Thus, the objective of this study is to evaluate anti-cancer effects of NLC-Citral in MDA MB-231 cells in vitro through the Annexin V, cell cycle, JC-1 and fluorometric assays. Additionally, this study is aimed to effects of NLC-Citral in reducing the tumor weight and size in 4T1 induced murine breast cancer model. Results showed that NLC-Citral induced apoptosis and G2/M arrest in MDA MB-231 cells. Furthermore, a prominent anti-metastatic ability of NLC-Citral was demonstrated in vitro using scratch, migration and invasion assays. A significant reduction of migrated and invaded cells was observed in the NLC-Citral treated MDA MB-231 cells. To further evaluate the apoptotic and anti-metastatic mechanism of NLC-Citral at the molecular level, microarray-based gene expression and proteomic profiling were conducted. Based on the result obtained, NLC-Citral was found to regulate several important signaling pathways related to cancer development such as apoptosis, cell cycle, and metastasis signaling pathways. Additionally, gene expression analysis was validated through the targeted RNA sequencing and real-time polymerase chain reaction. In conclusion, the NLC-Citral inhibited the proliferation of breast cancer cells in vitro, majorly through the induction of apoptosis, anti-metastasis, anti-angiogenesis potentials, and reducing the tumor weight and size without altering the therapeutic effects of citral.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nordin N,Yeap SK,Rahman HS,Zamberi NR,Abu N,Mohamad NE,How CW,Masarudin MJ,Abdullah R,Alitheen NBdoi
10.1038/s41598-018-38214-xsubject
Has Abstractpub_date
2019-02-07 00:00:00pages
1614issue
1issn
2045-2322pii
10.1038/s41598-018-38214-xjournal_volume
9pub_type
杂志文章abstract：:The availability of genotyping assays has allowed the detailed evaluation of cattle genetic diversity worldwide. However, these comprehensive studies did not include some local European populations, including autochthonous Italian cattle. In this study, we assembled a large-scale, genome-wide dataset of single nucleot...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71375-2
更新日期：2020-09-03 00:00:00
abstract：:Uric acid (UA) has been proposed as an important risk factor for cardiovascular and renal morbidity. We conducted an interventional trial to assess effects of altered salt intake on plasma and urine UA levels and the relationship between UA levels and salt sensitivity in humans. Ninety subjects (18-65 years old) were ...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-018-20048-2
更新日期：2018-01-23 00:00:00
abstract：:In the surface-enhanced fluorescence (SEF) process, it is well known that the plasmonic nanostructure can enhance the light emission of fluorescent emitters. With the help of atomic force microscopy, a hybrid system consisting of a fluorescent nanodiamond and a gold nanoparticle was assembled step-by-step for in situ ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22019-z
更新日期：2018-02-26 00:00:00
abstract：:As a fundamental property of pressure-induced amorphization (PIA) in ice and ice-like materials (notably α-quartz), the occurrence of mechanical instability can be related to violation of Born criteria for elasticity. The most outstanding elastic feature of α-quartz before PIA has been experimentally reported to be th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10810
更新日期：2015-06-23 00:00:00
abstract：:This study investigates magnetic ordering temperature in nano- and mesoscale structural features in an iron arsenide. Although magnetic ground states in quantum materials can be theoretically predicted from known crystal structures and chemical compositions, the ordering temperature is harder to pinpoint due to potent...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56301-5
更新日期：2019-12-27 00:00:00
abstract：:Administration of lipopolysaccharide (LPS) by various routes produces profound inflammatory pain hypersensitivity. However, the molecular events that induce this response remain largely uncharacterized. In the present study, we sought to elucidate the role of the Rho/Rho kinase (ROCK) pathway in the release of tumor n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14553
更新日期：2015-09-29 00:00:00
abstract：:Downstream analysis of genomic and transcriptomic sequence data is often executed by functional annotation that can be performed by various bioinformatics tools and biological databases. However, a full fast integrated tool is not available for such analysis. Besides, the current available software is not able to prod...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20211-9
更新日期：2018-01-29 00:00:00
abstract：:Alveolar type II epithelial cells (ATII) are instrumental in early wound healing in response to lung injury, restoring epithelial integrity through spreading and migration. We previously reported in separate studies that focal adhesion kinase-1 (FAK) and the chemokine receptor CXCR4 promote epithelial repair mechanism...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02204-2
更新日期：2017-05-19 00:00:00
abstract：:The combination of navigation and robotics in spine surgery has the potential to accurately identify and maintain bone entry position and planned trajectory. The goal of this study was to examine the feasibility, accuracy and efficacy of a new robot-guided system for semi-automated, minimally invasive, pedicle screw p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64462-x
更新日期：2020-05-05 00:00:00
abstract：:Psoriasis is a chronic, relapsing inflammatory skin disease. The pathogenesis of psoriasis is complex and has not been fully understood. C10orf99 was a recently identified human antimicrobial peptide whose mRNA expression is elevated in psoriatic human skin samples. In this study, we investigated the functional roles ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26996-z
更新日期：2018-06-05 00:00:00
abstract：:Transgenic plants producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are useful for pest control, but their efficacy is reduced when pests evolve resistance. Here we examined the mechanism of resistance to Bt toxin Cry1Ac in the laboratory-selected LF5 strain of the cotton bollworm, Helicov...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07219
更新日期：2014-11-27 00:00:00
abstract：:Fitness-related costs of evolving insecticide resistance have been reported in a number of insect species, but the interplay between evolutionary adaptation to insecticide pressure and variable environmental conditions has received little attention. We provisioned nymphs from three German cockroach (Blattella germanic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28731
更新日期：2016-06-27 00:00:00
abstract：:Natural killer (NK) cells are essential for immunosurveillance against transformed cells. Transient receptor potential melastatin 2 (TRPM2) is a Ca(2+)-permeable cation channel gated by ADP-ribose (ADPR). However, the role of TRPM2-mediated Ca(2+) signaling in the antitumor response of NK cells has not been explored. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09482
更新日期：2015-03-25 00:00:00
abstract：:Gene expression regulation is a complex and highly organized process involving a variety of genomic factors. It is widely accepted that differences in gene expression can contribute to the phenotypic variability between species, and that their interpretation can aid in the understanding of the physiologic variability....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12163
更新日期：2015-07-16 00:00:00
abstract：:RNA-based three-way junctions (3WJs) are naturally occurring structures found in many functional RNA molecules including rRNA, tRNA, snRNA and ribozymes. 3WJs are typically characterized as resulting from an RNA molecule folding back on itself in cis but could also form in trans when one RNA, for instance a microRNA b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45393
更新日期：2017-03-28 00:00:00
abstract：:A growing bacterium typically divides into two genetically identical and morphologically similar sister cells and eventually gives rise to a clonal population. Nevertheless, significant phenotypic differentiation among isogenic cells frequently occurs, with the resulting heterogeneity in cellular behavior often ensuri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08660-0
更新日期：2017-08-16 00:00:00
abstract：:Protein electrochemistry represents a powerful technique for investigating the function and structure of proteins. Currently available biochemical assays provide limited information related to the conformational state of proteins and high costs. This work provides novel insights into the electrochemical investigation ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53994-6
更新日期：2019-11-22 00:00:00
abstract：:Ballooning degeneration (BD) of hepatocytes is a distinguishing histological feature associated with the progression of nonalcoholic fatty liver disease (NAFLD). Under the assumption that NAFLD severity is associated with metabolic-stress we explored the hypothesis that heat shock 27 kDa protein 1 (HSP27), a protein c...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/srep22528
更新日期：2016-03-03 00:00:00
abstract：:A Maxwell fisheye lens using parallel plate index grading is presented in this study to develop a passive retrodirective antenna array. As a proof-of-concept a design frequency of 10 GHz was selected for fabrication and experiment. The design principals of the lens are discussed, which enables 85% energy flow at the d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52779-1
更新日期：2019-11-07 00:00:00
abstract：:Cattleyak are interspecific hybrids between cattle and yak, exhibiting the same prominent adaptability as yak and much higher performances than yak. However, male infertility of cattleyak resulted from spermatogenic arrest has greatly restricted their effective utilization in yak breeding. In past decades, much work h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18607-0
更新日期：2018-01-12 00:00:00
abstract：:This paper reports on 3D phase field simulations of IMC growth in Co/Sn and Cu/Sn solder systems. In agreement with experimental micrographs, we obtain uniform growth of the CoSn3 phase in Co/Sn solder joints and a non-uniform wavy morphology for the Cu6Sn5 phase in Cu/Sn solder joints. Furthermore, simulations were p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51179-9
更新日期：2019-10-16 00:00:00
abstract：:This study aimed to compare the effects of volatile anesthesia and total intravenous anesthesia (TIVA) on syndecan-1 shedding in patients with gastric cancer undergoing minimally invasive gastrectomy. Patients were randomly assigned to either the Volatile (n = 68) or the TIVA (n = 68) group. Anesthesia was maintained ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81012-1
更新日期：2021-01-15 00:00:00
abstract：:For a number of years nanomaterials have been continuously devised and comprehensively investigated because of the growing demand for them and their multifarious applications, especially in medicine. This paper reports on the properties of SrF2 nanoparticles (NPs) for applications in biomedicine, showing effective way...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45025-1
更新日期：2019-06-17 00:00:00
abstract：:Spin injection, manipulation and detection are the integral parts of spintronics devices and have attracted tremendous attention in the last decade. It is necessary to judiciously choose the right combination of materials to have compatibility with the existing semiconductor technology. Conventional metallic magnets w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05588
更新日期：2014-07-07 00:00:00
abstract：:It is known that patients with chronic obstructive pulmonary disease experience dyspnea during unsupported arm exercise (UAE). We examined the respiratory variables in during a hair-washing motion for healthy young people requiring the UAE to find the effects across gender, motion, and speed. In this study, 33 healthy...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-020-69945-5
更新日期：2020-08-04 00:00:00
abstract：:Computed Tomography derived Fractional Flow Reserve (CTFFR) is an emerging non-invasive imaging modality to assess functional significance of coronary stenosis. We performed a meta-analysis to compare the diagnostic performance of CTFFR to invasive Fractional Flow reserve (FFR). Electronic search was performed to iden...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-018-29910-9
更新日期：2018-08-01 00:00:00
abstract：:At mid-ocean ridges volcanism generally decreases with spreading rate but surprisingly massive volcanic centres occur at the slowest spreading ridges. These volcanoes can host unexpectedly strong earthquakes and vigorous, explosive submarine eruptions. Our understanding of the geodynamic processes forming these volcan...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42949
更新日期：2017-02-20 00:00:00
abstract：:Microorganisms in vertebrate guts have been recognized as important symbionts influencing host life. However, it remains unclear about the gut microbiota in long-distance migratory Anseriformes herbivores, which could be functionally important for these wetland-dependent animals. We collected faeces of the greater whi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32655
更新日期：2016-09-07 00:00:00
abstract：:We aimed to systematically investigate the potential association of matrix metalloproteinase (MMP)-9, -3, -2, and -8 gene polymorphisms with susceptibility to periodontitis using meta-analysis. A literature search in PubMed, Embase, and Web of Science was conducted to obtain relevant publications. Finally a total of 1...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep24812
更新日期：2016-04-20 00:00:00
abstract：:In response to ER stress, activating transcription factor 6 (ATF6) traffics from ER to Golgi apparatus where it is activated by cleavage before being translocated as transcription factor to the cell nucleus. In this work we describe ATF6α as a newly target of the aspirin metabolite sodium salicylate (NaSal). NaSal tre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09500-x
更新日期：2017-08-23 00:00:00
