Abstract:
:Notch1 is regulated by E3 ubiquitin ligases, with proteasomal degradation of the Notch intracellular domain affecting the transcription of target genes. cAMP response element-binding protein (CREB) mediates the transcription of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA). We assessed the relationship between HBV cccDNA and Notch signaling activities. HBV cccDNA levels and relative gene expression were evaluated in HBV-replicating cells treated with Jagged1 shRNA and a γ-secretase inhibitor. The effects of these factors in surgically resected clinical samples were also assessed. Notch inhibition suppressed HBV cccDNA and CREB-related expression but increased ITCH and NUMB levels. Proteasome inhibitor augmented HBV cccDNA, restored Notch and CREB expression, and inhibited ITCH and NUMB function. Increased HBV cccDNA was observed after ITCH and NUMB blockage, even after treatment with the adenylate cyclase activator forskolin; protein kinase A (PKA) inhibitor had the opposite effect. Notch activation and E3 ligase inactivation were observed in HBV-positive cells in clinical liver tissue. Collectively, these findings reveal that Notch signaling activity facilitates HBV cccDNA transcription via CREB to trigger the downstream PKA-phospho-CREB cascade and is regulated by E3 ubiquitin ligase-modulation of the Notch intracellular domain.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wang Z,Kawaguchi K,Honda M,Hashimoto S,Shirasaki T,Okada H,Orita N,Shimakami T,Yamashita T,Sakai Y,Mizukoshi E,Murakami S,Kaneko Sdoi
10.1038/s41598-018-38139-5subject
Has Abstractpub_date
2019-02-07 00:00:00pages
1621issue
1issn
2045-2322pii
10.1038/s41598-018-38139-5journal_volume
9pub_type
杂志文章abstract::We demonstrate fluorescent Fe embedded magnetic nanodiamonds by ion implantation and two-step annealing. The diamond characteristics with a highly ordered core and a graphite surface layer are maintained after the implantation process. After the two-step annealing process, a bright red fluorescence associated with nit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37820-z
更新日期:2019-02-04 00:00:00
abstract::We demonstrated modulation of charge carrier densities in all-solution-processed organic field-effect transistors (OFETs) by modifying the injection properties with self-assembled monolayers (SAMs). The all-solution-processed OFETs based on an n-type polymer with inkjet-printed Ag electrodes were fabricated as a test ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46365
更新日期:2017-04-12 00:00:00
abstract::Current control of insect pests relies on chemical insecticides, however, insecticide resistance development by pests is a growing concern in pest management. The main mechanisms for insecticide resistance typically involve elevated activity of detoxifying enzymes and xenobiotic transporters that break-down and excret...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01961-4
更新日期:2017-05-11 00:00:00
abstract::Complex associations exist between inflammation and thrombosis, with the inflammatory state tending to promote coagulation. Fibrinogen, an acute phase protein, has been shown to interact with the amyloidogenic ß-amyloid protein of Alzheimer's disease. However, little is known about the association between fibrinogen a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39056-x
更新日期:2019-02-28 00:00:00
abstract::In this study we characterise three tandem promoters (PR1-1, PR1-2 and PR1-3) within the PR1 regulatory region of the Escherichia coli kps capsule gene cluster. Transcription from promoter PR1-2 was dependent on the activity of the upstream promoter PR1-1, which activated PR1-2 via transcription coupled DNA supercoili...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17891-0
更新日期:2017-12-20 00:00:00
abstract::We propose a new method to extend the path length tunability of rotary delay-lines. This method was shown to achieve a duty cycle of >80% and repetition rates of over 40 kHz. The new method relies on a new multi-segmented micro-machined mirror and serial injection of a single reflection onto separate segments of this ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09576-5
更新日期:2017-08-24 00:00:00
abstract::Anti-PD-1 antibodies revolutionized the treatment of advanced melanoma patients. However, one out of three do not respond to this therapy, with an overall poor prognosis. Identification of predictive biomarkers in patients receiving immune-based therapies is necessary for minimizing risk of toxicity and optimizing pat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72711-2
更新日期:2020-10-19 00:00:00
abstract::The PIM1 serine/threonine protein kinase mediates growth factor and survival signalling, and cooperates potently with c-MYC during tumorigenesis. PIM1 is overexpressed in many human cancers and is a promising target for drug development. PIM1 levels are regulated mainly through cytokine-induced transcription and prote...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03775-w
更新日期:2017-06-15 00:00:00
abstract::Ice-albedo feedback due to the albedo contrast between water and ice is a major factor in seasonal sea ice retreat, and has received increasing attention with the Arctic Ocean shifting to a seasonal ice cover. However, quantitative evaluation of such feedbacks is still insufficient. Here we provide quantitative eviden...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08467-z
更新日期:2017-08-15 00:00:00
abstract::Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action for many therapeutic antibodies. A therapeutic immunoglobulin (Ig) G1 monoclonal antibody lost more than half of its ADCC activity after heat stress at 40 °C for 4 months. Size-exclusion and ion-exchange chromatography were used t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-57184-2
更新日期:2020-01-15 00:00:00
abstract::Electroporation (EP), the increase of cell membrane permeability due to the application of electric pulses, is a universal phenomenon with a broad range of applications. In medicine, some of the foremost EP-based tumor treatments are electrochemotherapy (ECT), irreversible electroporation, and gene electrotransfer (GE...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79858-y
更新日期:2021-01-14 00:00:00
abstract::Increased proteolytic activity has been widely associated with skeletal muscle atrophy. However, elevated proteolysis is also critical for the maintenance of cellular homeostasis by disposing cytotoxic proteins and non-functioning organelles. We recently demonstrated that exercise activates autophagy and re-establishe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30365-1
更新日期:2018-08-07 00:00:00
abstract::Since the propagation of plant viruses depends on various host susceptibility factors, deficiency in them can prevent viral infection in cultivated and model plants. Recently, we identified the susceptibility factor Essential for poteXvirus Accumulation 1 (EXA1) in Arabidopsis thaliana, and revealed that EXA1-mediated...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42400-w
更新日期:2019-04-11 00:00:00
abstract::During the last decades, atmospheric nitrogen loading in mountain ranges of the Northern Hemisphere has increased substantially, resulting in high nitrate concentrations in many lakes. Yet, how increased nitrogen has affected denitrification, a key process for nitrogen removal, is poorly understood. We measured actual...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59759-w
更新日期:2020-02-20 00:00:00
abstract::Ultraviolet radiation (UVR) is a major environmental genotoxic agent. In skin, it can lead to the formation of mutagenic DNA damage. Several mechanisms are in place to prevent the conversion of these DNA damage into skin cancer-driver mutations. An important mutation prevention mechanism is the programmed cell death, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75873-1
更新日期:2020-11-03 00:00:00
abstract::Wind erosion is a huge challenge for ecologists to stabilize sand dunes and to change them into stable productive ecosystems. In order to better understand its role in the process of ecological restoration, the sediment grain-size characteristics of compound sand barrier were evaluated through field experimental obser...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59538-7
更新日期:2020-02-13 00:00:00
abstract::Brain insulin sensitivity is an important link between metabolism and cognitive dysfunction. Intranasal insulin is a promising tool to investigate central insulin action in humans. We evaluated the acute effects of 160 U intranasal insulin on resting-state brain functional connectivity in healthy young adults. Twenty-...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-017-01907-w
更新日期:2017-05-09 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-65027-8
更新日期:2020-05-20 00:00:00
abstract::Pyrazinamide (PZA) is an important first-line drug in all existing and new tuberculosis (TB) treatment regimens. PZA-resistance in M. tuberculosis is increasing, especially among M/XDR cases. Noted issues with PZA Drug Susceptibility Testing (DST) have driven the search for alternative tests. This study provides a com...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03452-y
更新日期:2017-06-19 00:00:00
abstract::We present a proposal to realize the quantum Zeno effect (QZE) and quantum Zeno-like effect (QZLE) in a proximal (13)C nuclear spin by controlling a proximal electron spin of a nitrogen vacancy (NV) center. The measurement is performed by applying a microwave pulse to induce the transition between different electronic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17615
更新日期:2015-12-01 00:00:00
abstract::Land use change is one of the important aspects of the regional ecological restoration research. With remote sensing (RS) image in 2003, 2007 and 2012, using geographic information system (GIS) technologies, the land use pattern changes in Yimeng Mountain ecological restoration area in China and its driving force fact...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11038
更新日期:2015-06-05 00:00:00
abstract::Environmental contamination of arsenic (As) and its accumulation in rice (Oryza sativa L.) is of serious human health concern. In planta speciation of As is an important tool to understand As metabolism in plants. In the present study, we investigated root to shoot As translocation and speciation in rice exposed to in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40522
更新日期:2017-01-17 00:00:00
abstract::Colour polymorphism may be maintained within a population by disruptive-selection. One hypothesis proposes that different morphs are adapted to different ambient light conditions, with lighter morphs having a selective advantage in bright conditions and darker morphs having advantages in darker conditions. The mechani...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07829-x
更新日期:2017-08-22 00:00:00
abstract::Breast cancer is the leading cause of cancer death among women worldwide. Like other cancers, mammary carcinoma progression involves acidification of the tumor microenvironment, which is an important factor for cancer detection and treatment strategies. However, the effects of acidity on mammary carcinoma cell morphol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78989-6
更新日期:2020-12-15 00:00:00
abstract::Patients with end stage renal disease (ESRD) suffer high mortality from arrhythmias linked to fibrosis, but are contraindicated to late gadolinium enhancement magnetic resonance imaging (MRI). We present a quantitative method for gadolinium-free cardiac fibrosis imaging using magnetization transfer (MT) weighted MRI, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35394-4
更新日期:2018-11-19 00:00:00
abstract::The capacity of marine organisms to adapt and/or acclimate to climate change might differ among distinct populations, depending on their local environmental history and phenotypic plasticity. Kelp forests create some of the most productive habitats in the world, but globally, many populations have been negatively impa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-82094-7
更新日期:2021-01-28 00:00:00
abstract::Analyzing drug-drug interactions may unravel previously unknown drug action patterns, leading to the development of new drug discovery tools. We present a new approach to analyzing drug-drug interaction networks, based on clustering and topological community detection techniques that are specific to complex network sc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32745
更新日期:2016-09-07 00:00:00
abstract::In this paper, we used a facile ball milling, microwave radiation and heating treatment method to achieve the surface modification of TiO2 with low g-C3N4 concentration, and a multilayer heterojunction composite with TiO2 as the main part and g-C3N4 as the modification agent was obtained. The obtained materials were a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42438-w
更新日期:2019-04-11 00:00:00
abstract::Atmosphere-ocean coupling effect on the frequency distribution of tropical cyclones (TCs) and its future change is studied using an atmosphere and ocean coupled general circulation model (AOGCM). In the present climate simulation, the atmosphere-ocean coupling in the AOGCM improves biases in the AGCM such as the polew...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29800
更新日期:2016-07-15 00:00:00
abstract::Recent successes in malaria control have been largely attributable to the deployment of insecticide-based vector control tools such as bed nets and indoor residual spraying. Pyrethroid-treated bed nets are acutely neurotoxic to mosquitoes, inducing symptoms such as loss of coordination, paralysis, and violent spasms. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46674
更新日期:2017-04-25 00:00:00