Abstract:
INTRODUCTION:Stem cells from the apical papilla (SCAPs) possess strong odonto/osteogenic differentiation potential. This study investigated the effect of cyclic adenosine monophosphate (cAMP) on odonto/osteogenic differentiation of SCAPs and the underlining interplay between cAMP and transforming growth factor beta 1 (TGF-β1). METHODS:SCAPs were stimulated with an activator of cAMP (forskolin) in the presence of either TGF-β1 or a TGF-β1 inhibitor. The amounts of calcium mineral deposition and alkaline phosphatase activity were determined. Quantitative real-time polymerase chain reaction was performed to elucidate cAMP on the TGF-β1-mediated odonto/osteogenic differentiation of SCAPs. The effect of cAMP on the phosphorylation of Smad2/Smad3 and extracellular-regulated kinase (ERK)/P38 induced by TGF-β1 was analyzed by Western blotting. RESULTS:Cotreatment with forskolin and a TGF-β1 inhibitor enhanced alkaline phosphatase activity and deposition of calcium minerals in SCAPs. Moreover, the TGF-β1 inhibitor synergized the effect of forskolin on the expression of type I collagen and runt-related transcription factor 2. The results of Western blotting revealed that forskolin attenuated the unregulated expression of the phosphorylation of Smad3 and ERK induced by TGF-β1, and a cAMP inhibitor (H89) antagonized this effect. CONCLUSIONS:This study showed that cAMP signaling exerts its up-regulating effects on the odonto/osteogenic differentiation of SCAPs by interfering with TGF-β1 signaling via inhibiting Smad3 and ERK phosphorylation.
journal_name
J Endodjournal_title
Journal of endodonticsauthors
Zhang J,Zhang CF,Li QL,Chu CHdoi
10.1016/j.joen.2018.10.008subject
Has Abstractpub_date
2019-02-01 00:00:00pages
150-155issue
2eissn
0099-2399issn
1878-3554pii
S0099-2399(18)30691-5journal_volume
45pub_type
杂志文章abstract:INTRODUCTION:An evaluation was made of the connective tissue reaction in rats after subcutaneous implantation of methacrylate resin-based sealers (EndoREZ [Ultradent Products, Inc, South Jordan, UT] with a polymerization accelerator and RealSeal [Sybron Dental Specialties, Orange, CA]) and Pulp Canal Sealer (Sybron Den...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2010.06.019
更新日期:2010-09-01 00:00:00
abstract::This study aimed to investigate periapical exudate neutrophil elastase (NE) and prostaglandin E2 (PGE2) levels and their relationships with clinical symptoms, and to determine the changes of their levels following first treatment visit. Periapical exudate samples were collected from the canals of 34 nonvital single-ro...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000158010.43884.59
更新日期:2005-11-01 00:00:00
abstract:INTRODUCTION:In recent years, the inflammasome has been determined to play an important role in inflammatory diseases. However, the role of the inflammasome in pulpitis remains unclear. Absent in melanoma 2 (AIM2) is a type of inflammasome that recognizes cytosolic double stranded DNA and forms a caspase-1-activating i...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2013.07.003
更新日期:2013-11-01 00:00:00
abstract:INTRODUCTION:Many anatomical variations can occur within the mandibular first molar. Commonly, 3-4 canals are located, but as many as 6-7 canals have been reported. METHODS:This report describes a case of a mandibular first molar with 6 separate canals (3 mesial and 3 distal) that was instrumented with conventional ha...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.03.005
更新日期:2011-06-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the effect of chitosan-hydroxyapatite precursor (C-HA) nanocomplex conditioning on the chemical modifications at the tricalcium silicate sealer-dentin interface using time-of-flight secondary ion mass spectrometry. METHODS:Dentin slabs from human premolar root den...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.08.011
更新日期:2019-12-01 00:00:00
abstract::Dental pulp is a soft mesenchymal tissue densely innervated by afferent (sensory) fibers, sympathetic fibers, and parasympathetic fibers. This complexity in pulp innervation has motivated numerous investigations regarding how these 3 major neuronal systems regulate pulp physiology and pathology. Most of this research ...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/j.joen.2008.03.010
更新日期:2008-07-01 00:00:00
abstract::The aim of this study was to investigate fracture patterns occurring when a human upper central incisor is subjected to impact loadings at various angles. A two-dimensional finite element (FE) model of the maxillary incisor and surrounding tissues was established. The structural damping factor for the tooth was then c...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/01.don.0000157992.29221.41
更新日期:2005-10-01 00:00:00
abstract:INTRODUCTION:This in vitro study compared the performance of the XP-endo Shaper (XP; FKG, La Chaux-de-Fonds, Switzerland) in curved canals when used with a higher speed (3000 rpm) without a glide path with the manufacturer's protocol. METHODS:Twenty extracted mandibular molars with separate mesial curved canals were m...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.10.014
更新日期:2020-01-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to evaluate the influence of pH that is due to setting reaction of Biodentine, glass ionomer cement (GIC), and intermediate restorative material (IRM) on transforming growth factor-β1 (TGF-β1) release and on the fibrin architecture of platelet-rich fibrin (PRF). METHODS:PRF w...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.02.009
更新日期:2016-05-01 00:00:00
abstract::Conventional and surgical endodontic management were performed on a two-rooted maxillary central incisor with a periapical lesion. Anatomical complexity of the tooth lead to perforation in the distal root during conventional treatment, thus a surgical approach was taken. A 4-month postoperative radiograph shows repair...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200306000-00011
更新日期:2003-06-01 00:00:00
abstract:INTRODUCTION:The authors conducted a prospective, randomized, single-blind study to determine the degree of pulpal anesthesia obtained with a primary infiltration of 1 cartridge of articaine in the incisive/mental nerve region of the mandibular second premolar and to determine the anesthetic efficacy of a repeat artica...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2012.11.039
更新日期:2013-03-01 00:00:00
abstract:INTRODUCTION:Magnetic resonance imaging (MRI) has the potential to aid in determining the presence and extent of cracks/fractures in teeth because of better contrast without ionizing radiation. The objectives were to develop MRI criteria for root crack/fracture identification and to establish reliability and accuracy i...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2019.03.008
更新日期:2019-06-01 00:00:00
abstract:INTRODUCTION:Endodontic diagnostic tests are often used clinically to assess pulp status as a basis for the diagnosis and determination of whether root canal treatment (RCT) is indicated. Response to cold and pain on percussion are 2 common tests, yet their validity in identifying nonvital pulp in regular dental practi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.03.016
更新日期:2016-06-01 00:00:00
abstract:INTRODUCTION:Krüppel-like factor 4 (KLF4) plays an important role in cytodifferentiation and proliferation. Our previous study showed that KLF4 was specifically expressed in polarizing and elongating odontoblasts. However, the role of KLF4 in odontoblast differentiation was still unknown. The purpose of this study was ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.03.030
更新日期:2011-07-01 00:00:00
abstract::Sixty-eight standardized human root specimens were infected with Enterococcus faecalis for 3 wk after removal of the smear layer. After 3 wk of infection, the smear layer was reformed and in half of the specimens, the smear layer was again removed. Aqueous Ca(OH)2 paste and silicone oil based Ca(OH)2 paste were used a...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1097/00004770-200105000-00004
更新日期:2001-05-01 00:00:00
abstract:INTRODUCTION:The retrieval of fractured instruments from root canals has been largely reported in the literature. However, a less complex retrieval method that causes minimum damage to the dental structures is needed. The aim of this article is to introduce a new concept of retrieval of fractured instruments from root ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2009.11.024
更新日期:2010-04-01 00:00:00
abstract:INTRODUCTION:This study aimed to investigate the roles of ephrinB2 in stabilizing vascularlike structures generated by stem cells from apical papilla (SCAPs) and human umbilical vein endothelial cells (HUVECs). METHODS:HUVECs were seeded alone or with SCAPs concurrently or 12 hours later. Angiogenesis and ephrinB2 pho...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.05.012
更新日期:2016-09-01 00:00:00
abstract:INTRODUCTION:Compelling evidence pinpoints that pulp tissue engineering after the transplantation of stem cells is possible. Although intriguing, severe problems regarding clinical feasibility remain. Cell homing has been proposed as a viable alternative in which dentin-derived growth factors in a conducive scaffold ma...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2018.02.011
更新日期:2018-06-01 00:00:00
abstract::This study compared the apical seal produced by four obturation techniques. Sixty-four extracted human teeth were prepared and obturated using lateral condensation of gutta-percha that was either unmodified or was dipped in chloroform, eucalyptol, or eucapercha paste. After storage in normal saline and 0.02% azide sol...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(89)80158-X
更新日期:1989-12-01 00:00:00
abstract:INTRODUCTION:The purpose of this study was to visualize the stresses and strain distribution patterns in ProTaper Universal F2 files (Dentsply Maillefer, Ballaigues, Switzerland) and to establish the stress- and strain-curvature relationship for this instrument under various conditions by using a dynamic, three-dimensi...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.06.003
更新日期:2011-10-01 00:00:00
abstract::This study assessed in vitro the diagnostic ability of cone beam computed tomography (CBCT) scans with different voxel resolutions in the detection of simulated external root resorption (ERR). For that purpose, 59 teeth were viewed through iCAT tomography (Imaging Sciences International, Inc, Hatfield, PA) following t...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.11.005
更新日期:2009-02-01 00:00:00
abstract::This report presents a rare case of odontogenic cutaneous sinus tract caused by a vertical root fracture. The root-fractured molar was probably caused by an accidental blow by a cow 3 years ago. Despite all treatments by physicians and surgeons for nearly 1 year, the sinus tract recurred repeatedly. After diagnosis an...
journal_title:Journal of endodontics
pub_type: 杂志文章,评审
doi:10.1016/s0099-2399(06)81130-1
更新日期:1997-09-01 00:00:00
abstract:INTRODUCTION:The study was designed as a randomized double-blind trial to evaluate the anesthetic efficacy of 4% articaine with 1:100,000 epinephrine in inferior alveolar nerve block (IANB) and infiltration anesthetic techniques to anesthetize mandibular molars with irreversible pulpitis. METHODS:The study was compose...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2011.09.009
更新日期:2011-12-01 00:00:00
abstract::The present survey was conducted to obtain answers to some basic questions regarding the use of intracanal medications, root canal filling methods, and the intracanal breakage of instruments. A letter was sent to 300 endodontists listed in the 1995-1996 membership roster of the American Association of Endodontists. Ei...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(99)80306-9
更新日期:1999-12-01 00:00:00
abstract::The effects of various cleaning, chemical disinfection, and sterilization procedures on the cutting efficiency of endodontic instruments Unifile (De Trey, Bois Colombes, France), (Flexofile Maillefer, Ballaigues, Switzerland), and H-File (Maillefer)) were investigated. The cross-infection control treatment procedures ...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/S0099-2399(96)80059-8
更新日期:1996-12-01 00:00:00
abstract::This study compares the incidence, degree, and length of postoperative pain in 300 endodontically treated teeth, with and without apical patency, in relation to some diagnostic factors (vitality, presence of preoperative pain, group, and mandible of treated tooth). Of the questionnaires received back, apical patency w...
journal_title:Journal of endodontics
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joen.2008.11.014
更新日期:2009-02-01 00:00:00
abstract:INTRODUCTION:The study compared Twisted File (TF) with 3 traditionally manufactured systems to determine whether changes in the manufacturing process improved the cyclic fatigue resistance. METHODS:Four rotary file systems, (1) ProFile (PF), (2) Mtwo, (3) K3, and (4) TF, were tested in artificial canals with 45° and 9...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2011.12.034
更新日期:2012-04-01 00:00:00
abstract:INTRODUCTION:This finite element analysis study was aimed at evaluating the effects of rotary systems on stresses in photopolymerized resin root models with oval-shaped canals. METHODS:Among the data collected by computed tomographic imaging, a mandibular second premolar tooth with an oval-shaped canal was selected, r...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2016.05.007
更新日期:2016-08-01 00:00:00
abstract:INTRODUCTION:Dental pulp has limited capability to regenerate, which happens in the early stage of pulpitis. An ambiguous relationship exists; inflammation may impair or support pulp regeneration. Epigenetics, which is involved in cell proliferation and inflammation, could regulate human dental pulp cell (HDPCs) regene...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2014.01.031
更新日期:2014-08-01 00:00:00
abstract::The aim of this study was to investigate the extent of calcium ion (Ca(2+)) removal on root dentin after treatment with 1.25% NaOCl, 2.5% NaOCl, 5.25% NaOCl, 2% chlorhexidine (CHX), 2% iodine potassium iodide (IKI), and MTAD in 3 immersion time periods. Extracted human mandibular premolars were bisected longitudinally...
journal_title:Journal of endodontics
pub_type: 杂志文章
doi:10.1016/j.joen.2008.11.016
更新日期:2009-02-01 00:00:00