The Human Ocular Surface Fungal Microbiome.

Abstract:

Purpose:To enumerate the ocular surface fungal microbiome of healthy human eyes by using next-generation sequencing (NGS). Methods:Tarsal and fornix conjunctiva from the lower and upper lids of both eyes of healthy individuals were swabbed in duplicate separately. A total of 34 samples were collected from both the eyes of 17 individuals, which were used for the generation of ocular surface fungal microbiomes by NGS. Twenty-four swabs were used for the detection of culturable fungi by the conventional cultivable method. Microbiome generation involved DNA extraction, internal transcribed spacer 2 (ITS2) amplification, library preparation, amplicon sequencing, taxonomic assignment of sequences, diversity analyses, and identification of genera. Results:The cultivable method detected fungi in 3 out of 24 (12.5 %) ocular surface swabs, whereas NGS identified fungi in 25 of the 34 (73.5 %) swabs. In the cultivable method Aspergillus was the only genus detected, whereas NGS detected 65 distinct genera with 12 to 24 genera per microbiome. Genera Aspergillus, Setosphaeria, Malassezia, and Haematonectria were present in the 25 eyes in which fungi were detected. Alpha diversity in the two eyes was similar and sex had no effect, but Chao1 and Simpson indices were altered by age. Conclusions:This study explored the ocular surface fungal microbiome of healthy individuals using NGS and identified a greater degree of diversity of fungi than with the conventional cultivable method. It was observed that several fungal genera were associated with the healthy conjunctiva.

authors

Shivaji S,Jayasudha R,Sai Prashanthi G,Kalyana Chakravarthy S,Sharma S

doi

10.1167/iovs.18-26076

subject

Has Abstract

pub_date

2019-01-02 00:00:00

pages

451-459

issue

1

eissn

0146-0404

issn

1552-5783

pii

2723591

journal_volume

60

pub_type

杂志文章
  • Diverse mediators modulate the chloride ion fluxes that drive lacrimal fluid production.

    abstract:PURPOSE:To learn whether locally expressed and systemic mediators might modulate the cholinergically induced transepithelial Cl(-) fluxes that underlie lacrimal fluid production. METHODS:Reconstituted epithelial monolayers were exposed to a submaximal dose of the muscarinic agonist, carbachol (CCh), or to one of sever...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.12-10202

    authors: Selvam S,Mircheff AK,Yiu SC

    更新日期:2013-04-26 00:00:00

  • Development of VEP Vernier acuity and grating acuity in human infants.

    abstract:PURPOSE:To compare the developmental sequences of two basic measures of pattern vision, Vernier acuity and grating acuity, using steady state visual-evoked potentials (VEPs) and an analysis designed to isolate pattern-specific responses from those due to motion in the Vernier stimulus. METHODS:The authors recorded VEP...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Skoczenski AM,Norcia AM

    更新日期:1999-09-01 00:00:00

  • Reattachment of cultured human retinal pigment epithelium to extracellular matrix and human Bruch's membrane.

    abstract:PURPOSE:To determine the mechanism of reattachment of harvested human retinal pigment epithelium (RPE) to RPE-derived extracellular matrix and Bruch's membrane. METHODS:Confluent first-to third-passage human RPE were harvested from tissue culture and plated onto RPE-derived extracellular matrix or human Bruch's membra...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Ho TC,Del Priore LV

    更新日期:1997-05-01 00:00:00

  • The ocular hypotensive effects of demeclocycline, tetracycline and other tetracycline derivatives.

    abstract::Demeclocycline, tetracycline and other tetracycline derivatives lowered intraocular pressure (IOP) in rabbits following intravitreal injection, but the onset of this effect was not evident until 1 or more days after drug administration. Of the drugs tested, demeclocycline was the most active ocular hypotensive agent. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Wallace I,Krupin T,Stone RA,Moolchandani J

    更新日期:1989-07-01 00:00:00

  • Aqueous Humor Dynamics of the Brown-Norway Rat.

    abstract:Purpose:The study aimed to provide a quantitative description of aqueous humor dynamics in healthy rat eyes. Methods:One eye of 26 anesthetized adult Brown-Norway rats was cannulated with a needle connected to a perfusion pump and pressure transducer. Pressure-flow data were measured in live and dead eyes by varying p...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.17-22915

    authors: Ficarrotta KR,Bello SA,Mohamed YH,Passaglia CL

    更新日期:2018-05-01 00:00:00

  • Peptidergic stimulation and inhibition of lacrimal gland adenylate cyclase.

    abstract::Vasoactive intestinal peptide (VIP) and d-ala2-methionine enkephalinamide (DALA, a long-lasting enkephalin analogue) were used to investigate the peptidergic control of lacrimal gland function. To characterize the mechanism by which VIP stimulates and DALA inhibits lacrimal peroxidase secretion, the effect of these pe...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Cripps MM,Bennett DJ

    更新日期:1990-10-01 00:00:00

  • Transforming Growth Factor-β1-induced Human Subconjunctival Fibrosis is Mediated by MicroRNA 143/145 Expression.

    abstract:Purpose:To investigate the roles and pathways of microRNAs 143 and 145 in transforming growth factor (TGF)-β1-induced human subconjunctival fibrosis. Methods:Human tenon's capsule fibroblasts (HTFs) were obtained from a healthy eye. After treating cultured HTFs with TGF-β1, the expression of microRNAs 143 and 145 was ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.19-26797

    authors: Hwang YH,Jung SA,Lyu J,Kim YY,Lee JH

    更新日期:2019-05-01 00:00:00

  • Dietary ganglioside and long-chain polyunsaturated fatty acids increase ganglioside GD3 content and alter the phospholipid profile in neonatal rat retina.

    abstract:PURPOSE:During early development, the ganglioside composition of the retina changes significantly, in that GD3 becomes the primary ganglioside in the mammalian retina. Because gangliosides play an important role in neuronal cell differentiation and proliferation, this change in ganglioside profile may indicate retinal ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.04-1439

    authors: Park EJ,Suh M,Clandinin MT

    更新日期:2005-07-01 00:00:00

  • Switching of α-Catenin From Epithelial to Neuronal Type During Lens Epithelial Cell Differentiation.

    abstract:Purpose:Ocular lens fiber cell elongation, differentiation, and compaction are associated with extensive reorganization of cell adhesive interactions and cytoskeleton; however, our knowledge of proteins critical to these events is still evolving. This study characterizes the distribution pattern of neuronal-specific α-...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.17-21539

    authors: Maddala R,Rao PV

    更新日期:2017-07-01 00:00:00

  • Location and Gaze-Dependent Shift of Inferior Oblique Muscle Position: Anatomic Contributors to Vertical Strabismus Following Lower Lid Blepharoplasty?

    abstract:PURPOSE:This study investigated, using high-resolution magnetic resonance imaging (MRI), inferior oblique muscle (IO) position relative to the adnexa in normal controls, subjects with and without vertical strabismus following lower lid blepharoplasty, and subjects with other hypertropia. METHODS:Sagittal plane MRI was...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-15324

    authors: Shin SY,Demer JL

    更新日期:2015-04-01 00:00:00

  • Evidence for a major gene for cortical cataract.

    abstract:PURPOSE:To examine the possible presence of a major gene determining susceptibility to cortical cataract. METHODS:The percentage of the lens area involved with cortical opacity, summed over both eyes, was evaluated in 1275 individuals from the Beaver Dam Eye Study. After adjusting for the effects of age and sex, these...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Heiba IM,Elston RC,Klein BE,Klein R

    更新日期:1995-01-01 00:00:00

  • Validation of physiologic predictors of successful telescopic spectacle use in low vision.

    abstract::A group of 32 patients with low vision who were considered clinically appropriate candidates for visual rehabilitation with telescopic spectacles were prospectively studied before the first attempted use of these visual aids. Laboratory measurements were made of: (1) rotational head stability in pitch and yaw during q...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Demer JL,Goldberg J,Porter FI,Schmidt K

    更新日期:1991-09-01 00:00:00

  • The prevalence and incidence of epiretinal membranes in eyes with inactive extramacular CMV retinitis.

    abstract:PURPOSE:To determine the prevalence and incidence of epiretinal membranes (ERM) in eyes with inactive extramacular cytomegalovirus (CMV) retinitis in patients with acquired immune deficiency syndrome (AIDS). METHODS:A case-control report from a longitudinal multicenter observational study by the Studies of the Ocular ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,多中心研究

    doi:10.1167/iovs.14-14479

    authors: Kozak I,Vaidya V,Van Natta ML,Pak JW,May KP,Thorne JE,Studies of the Ocular Complications of AIDS Research Group.

    更新日期:2014-06-12 00:00:00

  • Flicker light-induced retinal vasodilation is unaffected by inhibition of epoxyeicosatrienoic acids and prostaglandins in humans.

    abstract:PURPOSE:To investigate the role of epoxyeicosatrienoic acids (EETs) and prostaglandins (PGs) in retinal blood vessel calibers and vasodilation during flicker light stimulation in humans. METHODS:Twelve healthy nonsmokers participated in a balanced crossover study. Oral fluconazole 400 mg and dispersible aspirin 600 mg...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,随机对照试验

    doi:10.1167/iovs.14-14947

    authors: Noonan JE,Dusting GJ,Nguyen TT,Jenkins AJ,Man RE,Best WJ,Dias DA,Jayasinghe NS,Roessner U,Lamoureux EL

    更新日期:2014-10-08 00:00:00

  • Stereoscopic Viewing Can Induce Changes in the CA/C Ratio.

    abstract:PURPOSE:Stereoscopic displays challenge the neural cross-coupling between accommodation and vergence by inducing a constant accommodative demand and a varying vergence demand. Stereoscopic viewing calls for a decrease in the gain of vergence accommodation, which is the accommodation caused by vergence, quantified by us...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-18854

    authors: Neveu P,Roumes C,Philippe M,Fuchs P,Priot AE

    更新日期:2016-08-01 00:00:00

  • A human lens model of cortical cataract: Ca2+-induced protein loss, vimentin cleavage and opacification.

    abstract:PURPOSE:Cortical cataract in humans is associated with Ca2+ overload and protein loss, and although animal models of cataract have implicated Ca2+-activated proteases in this process, it remains to be determined whether the human lens responds in this manner to conditions of Ca2+ overload. The purpose of these experime...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Sanderson J,Marcantonio JM,Duncan G

    更新日期:2000-07-01 00:00:00

  • High-resolution photoreceptor imaging in idiopathic macular telangiectasia type 2 using adaptive optics scanning laser ophthalmoscopy.

    abstract:PURPOSE:To study pathologic changes in the photoreceptors in eyes with idiopathic macular telangiectasia (MacTel) type 2 using adaptive optics scanning laser ophthalmoscopy (AO-SLO). METHODS:Thirteen eyes with nonproliferative MacTel type 2 and 10 normal eyes underwent a full ophthalmologic examination, spectral-domai...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-7251

    authors: Ooto S,Hangai M,Takayama K,Arakawa N,Tsujikawa A,Koizumi H,Oshima S,Yoshimura N

    更新日期:2011-07-25 00:00:00

  • Congenital stationary night blindness: an animal model.

    abstract::Electroretinographic studies of myctalopic Appaloosa horses demonstrated photopic and scotopic abnormalities similar to those in humans with congenital stationary night blindness (CSNB) of the Schubert-Bornschein type. The phototopic abnormalities consisted of reduced b-wave amplitudes and slower than normal b-wave im...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Witzel DA,Smith EL,Wilson RD,Aguirre GD

    更新日期:1978-08-01 00:00:00

  • Retinal nerve fiber layer normative classification by optical coherence tomography for prediction of future visual field loss.

    abstract:PURPOSE:To evaluate the utility of baseline Stratus optical coherence tomography (OCT; Carl Zeiss Meditec, Dublin, CA) retinal nerve fiber layer (RNFL) normative classification in the prediction of future visual field (VF) loss. METHODS:Eighty-eight eyes with suspected glaucoma with abnormal RNFL classification by Str...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-6246

    authors: Sung KR,Kim S,Lee Y,Yun SC,Na JH

    更新日期:2011-04-22 00:00:00

  • Vertical vergence adaptation produces an objective vertical deviation that changes with head tilt.

    abstract:PURPOSE:To document the cyclovertical ocular motor mechanism used for vertical fusion in healthy subjects, and to explore whether vertical vergence training in healthy individuals can produce objectively confirmed vertical deviations that change with head tilt, revealing a basic mechanism that can produce a pattern of ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.12-11368

    authors: Irsch K,Guyton DL,Ramey NA,Adyanthaya RS,Ying HS

    更新日期:2013-05-03 00:00:00

  • Clinical assessment of mirror artifacts in spectral-domain optical coherence tomography.

    abstract::PURPOSE. To investigate the characteristics of a spectral-domain optical coherence tomography (SD-OCT) image phenomenon known as the mirror artifact, calculate its prevalence, analyze potential risk factors, measure severity, and correlate it to spherical equivalent and central visual acuity (VA). METHODS. OCT macular...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-4057

    authors: Ho J,Castro DP,Castro LC,Chen Y,Liu J,Mattox C,Krishnan C,Fujimoto JG,Schuman JS,Duker JS

    更新日期:2010-07-01 00:00:00

  • Adult rabbit retinal transplants.

    abstract:PURPOSE:To study the survival of adult retinal grafts prepared in a physiologically optimized way. METHODS:Twenty-three rabbits received an adult full-thickness rabbit retinal transplant positioned under the host retina, using a vitrectomy technique. The transplants were prepared using a procedure based on a previousl...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Wassélius J,Ghosh F

    更新日期:2001-10-01 00:00:00

  • Spatial distribution of posterior pole choroidal thickness by spectral domain optical coherence tomography.

    abstract:PURPOSE:To study the spatial distribution of posterior pole choroidal thickness (CT) in healthy eyes using spectral domain optical coherence tomography (SD-OCT). METHODS:Fifty-nine eyes from 30 subjects with no retinal or choroidal disease were examined with high-definition (HD) OCT using macular volume cube scanning ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-8046

    authors: Ouyang Y,Heussen FM,Mokwa N,Walsh AC,Durbin MK,Keane PA,Sanchez PJ,Ruiz-Garcia H,Sadda SR

    更新日期:2011-09-01 00:00:00

  • Simple reaction times in color space: the influence of chromaticity, contrast, and cone opponency.

    abstract:PURPOSE:This study examined the influence of stimulus chromaticity on simple reaction times (RTs) to determine the stage of chromatic processing that is most influential in their generation. METHODS:Simple RTs were measured in response to the cosinusoidally ramped onset of small, equiluminant, colored Gaussian spots. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.02-0772

    authors: McKeefry DJ,Parry NR,Murray IJ

    更新日期:2003-05-01 00:00:00

  • Uveoscleral aqueous outflow in the rhesus monkey: importance of uveal reabsorption.

    abstract::The uveal absorption of aqueous humor at two different intraocular pressures was measured in rhesus monkeys by sampling vortex vein blood during anterior chamber perfusion of Ringer's solution containing both fluorescein and 125I albumin. At 20 mm. Hg, an excess of fluorescein equivalent in amount to 0.45 microliter/m...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Pederson JE,Gaasterland DE,MacLellan HM

    更新日期:1977-11-01 00:00:00

  • Lens-specific expression of a major histocompatibility complex class I molecule disrupts normal lens development and induces cataracts in transgenic mice.

    abstract:PURPOSE:Lens epithelial tissue does not normally express major histocompatibility complex (MHC) class I molecules. In addition, the mechanism of self-tolerance to intraocular antigens is unknown. To study the effect of class I expression in the lens, transgenic mice were produced that express an allo-MHC class I molecu...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Martin WD,Egan RM,Stevens JL,Woodward JG

    更新日期:1995-05-01 00:00:00

  • Effects of human epidermal growth factor on endothelial wound healing of human corneas.

    abstract::Paired human donor corneas (age, 73 +/- 12 yr), preserved in organ culture medium, were used to evaluate the effect of human epidermal growth factor (hEGF) on endothelial wound closure rate (WCR), on morphometric parameters (cell size, shape, and density), and on cell division in the wound area. The endothelium of the...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Hoppenreijs VP,Pels E,Vrensen GF,Oosting J,Treffers WF

    更新日期:1992-05-01 00:00:00

  • Distribution of pemphigus and pemphigoid antigens, laminin, and type IV collagen in corneal epithelium.

    abstract::Dog corneas were studied by indirect immunofluorescence with antibodies specific for pemphigus and pemphigoid antigens, laminin, and type IV collagen. The distribution of these antigens was similar to that observed in other squamous epithelia and skin. This study provides further evidence for close immunochemical simi...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Cowen SJ,Anhalt GJ,Wicha MS,Sugar A,Labib RS,Diaz LA

    更新日期:1981-12-01 00:00:00

  • Expression of soluble phototransduction-associated proteins in ground squirrel retina.

    abstract:PURPOSE:This study describes the expression and distribution of arrestin, phosducin, and recoverin in the cone-dominant retina of the ground squirrel Spermophilus tridecemlineatus. METHODS:mRNA expression was studied by blot hybridization of ground squirrel retinal RNA, with human and murine RNA as controls. The distr...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: von Schantz M,Szél A,van Veen T,Farber DB

    更新日期:1994-10-01 00:00:00

  • Association between polymorphisms of complement pathway genes and age-related macular degeneration in a Chinese population.

    abstract:PURPOSE:We assessed the association between complement pathway genes and age-related macular degeneration (AMD) in a Chinese population. METHODS:In a case-control study, 165 AMD patients and 216 unrelated controls were recruited from two hospitals in central China. We selected and genotyped six single nucleotide poly...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.12-10453

    authors: Wu L,Tao Q,Chen W,Wang Z,Song Y,Sheng S,Li P,Zhou J

    更新日期:2013-01-07 00:00:00