Abstract：
:Microcalcifications are vital mammographic indicators contributing to the early detection of up to 50% of non-palpable tumours and may also be valuable as prognostic markers. However, the precise mechanism by which they form remains incompletely understood. Following development of an in vitro model using human breast cancer cells lines cultured with a combination of mineralisation-promoting reagents, analysis of calcium deposition, alkaline phosphatase (ALP) activity and changes in expression of key genes was used to monitor the calcification process. Two cell lines were identified as successfully mineralising in vitro, MDA-MB-231 and SKBR3. Mineralising cell lines displayed higher levels of ALP activity that was further increased by addition of mineralisation promoting media. qPCR analysis revealed changes in expression of both pro- (RUNX2) and anti- (MGP, ENPP1) mineralisation genes. Mineralisation was suppressed by chelation of intracellular Ca2+ and inhibition of TRPM7, demonstrating a functional role for the channel in formation of microcalcifications. Increased Mg2+ was also found to effectively reduce calcium deposition. These results expand the number of human breast cancer cell lines with a demonstrated in vitro mineralisation capability, provide further evidence for the role of an active, cellular process of microcalcification formation and demonstrate for the first time a role for TRPM7 mediated Ca2+ transport.
journal_name
Sci Repjournal_title
Scientific reportsauthors
O'Grady S,Morgan MPdoi
10.1038/s41598-018-36496-9subject
Has Abstractpub_date
2019-01-24 00:00:00pages
542issue
1issn
2045-2322pii
10.1038/s41598-018-36496-9journal_volume
9pub_type
杂志文章abstract：:Early ontogenetic periods and postnatal maturation in organisms are sex-specifically sensitive to hypothalamic-pituitary-adrenal (HPA)-axis activities, related glucocorticoid secretions, and their effects on energy balance and homeostasis. Dietary polyunsaturated (PUFAs) and saturated (SFAs) fatty acids potentially pl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18978-4
更新日期：2018-01-11 00:00:00
abstract：:Uveitis, the pathologic condition of inflammation of the uvea, frequently leads to severe vision loss and blindness. S100A8 is a calcium-binding protein which mainly expresses in granulocytes and monocytes and plays a prominent role in the regulation of inflammatory processes and immune response. Here, we determined t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36140
更新日期：2016-10-27 00:00:00
abstract：:Tipping point dynamics are fundamental drivers for sustainable transition pathways of social-ecological systems (SES). Current research predominantly analyzes how crossing tipping points causes regime shifts, however, the analysis of potential transition pathways from these social and ecological tipping points is ofte...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59713-w
更新日期：2020-03-05 00:00:00
abstract：:Glomerular filtration rate (GFR) and urinary albumin excretion rate (UAER) are used to diagnose and classify the severity of chronic kidney disease. Total adiponectin (T-AN) and high molecular weight adiponectin (H-AN) assays were developed using the fully automated immunoassay system, HI-1000 and their significance o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72494-6
更新日期：2020-09-28 00:00:00
abstract：:Although Heme Oxygenase-1 (HO-1) induction in various forms of kidney injury is protective, its role in age-related renal pathology is unknown. In the ageing kidney there is nephron loss and lesions of focal glomerulosclerosis, interstitial fibrosis, tubular atrophy and arteriolosclerosis. Underlying mechanisms includ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62016-9
更新日期：2020-03-31 00:00:00
abstract：:This study investigated the clinicopathological characteristics of Henoch-Schönlein purpura nephritis (HSPN) in Chinese adult patients and analyzed the renal outcomes and prognostic risk factors for progression to end-stage renal disease (ESRD). Adult patients who had biopsy-proven HSPN were studied. Their clinicopath...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23638-2
更新日期：2018-04-03 00:00:00
abstract：:Unimolecular dual agonists for the glucagon-like peptide 1 receptor (GLP1R) and glucagon receptor (GCGR) are emerging as a potential new class of important therapeutics in type 2 diabetes (T2D). Reliable and quantitative assessments of in vivo occupancy on each receptor would improve the understanding of the efficacy ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73815-5
更新日期：2020-10-07 00:00:00
abstract：:Autistic spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impairment in social communication and restricted/repetitive behavior patterns or interests. Antagonists targeting histamine H3 receptor (H3R) are considered potential therapeutic agents for the therapeutic management of different brain...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31385-7
更新日期：2018-08-30 00:00:00
abstract：:Antimicrobial peptides, produced by innate immune system of hosts in response to invading pathogens, are capable of fighting against a spectrum of bacteria, viruses, fungi, parasites and cancer cells. Here, a recombinant silkworm AMP Bmattacin2 from heterologous expression is studied, indicating a broad spectrum of an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31149
更新日期：2016-08-09 00:00:00
abstract：:The iron binding capacities (IBC) of fulvic acid (FA) and humic acid (HA) were determined in the salinity range from 5 to 40. The results indicated that IBC decreased while salinity increased. In addition, dissolved iron (dFe), FA and HA were also determined along the Yangtze River estuary's increasing salinity gradie...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01533-6
更新日期：2017-05-03 00:00:00
abstract：:Toll-like receptors (TLRs), as innate immunity sensors, play critical roles in immune responses. Six SNPs of TLR3, TLR7, and TLR8 were genotyped to determine their associations with systemic lupus erythematosus (SLE) and clinical manifestations of SLE. TLR7 SNP rs3853839 was independently associated with SLE susceptib...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03792
更新日期：2014-01-21 00:00:00
abstract：:Wind speed and variability are the most critical climatic factors affecting sand/dust storms, which have not been sufficiently studied in the middle reaches of the Yarlung Tsangpo River (MYR). In this study, wind speed variability was investigated using the moving average over shifting horizon method (MASH), combined ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69392-2
更新日期：2020-07-29 00:00:00
abstract：:The incisive evaluation of psychological stress may be required to determine the exercise performance of stressed hosts. We investigated objective markers of psychological stress that reflect exercise performance, focusing on the neutrophil function. We used murine water-immersion restraint (WIR) stress for our assess...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41077-5
更新日期：2019-03-14 00:00:00
abstract：:This study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80764-6
更新日期：2021-01-14 00:00:00
abstract：:Severe sepsis or septic shock is one of the rising causes for mortality worldwide representing nearly 10% of intensive care unit admissions. Susceptibility to sepsis is identified to be mediated by innate pattern recognition receptors and responsive signaling pathways of the host. The c-Jun N-terminal Kinase (JNK)-med...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07214
更新日期：2014-11-27 00:00:00
abstract：:We present the continuous-time evolution of an online organism network from birth to death which crosses all organizational and temporal scales, from individual components through to the mesoscopic and entire system scale. These continuous-time data reveal a lifespan driven by punctuated, real-time co-evolution of the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22027-z
更新日期：2018-02-23 00:00:00
abstract：:Graphene has shown enormous potential for innovation in various research fields. The current chemical approaches based on exfoliation of graphite via graphite oxide (GO) are potential for large-scale synthesis of graphene but suffer from high cost, great operation difficulties, and serious waste discharge. We report a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03439
更新日期：2013-12-06 00:00:00
abstract：:Fuelled by a desire for greater connectivity, networked systems now pervade our society at an unprecedented level that will affect it in ways we do not yet understand. In contrast, nature has already developed efficient networks that can instigate rapid response and consensus when key elements are stimulated. We prese...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53942-4
更新日期：2019-11-26 00:00:00
abstract：:Cell counts decrease with sediment depth. Typical explanations consider limiting factors such as water availability and chemistry, carbon source, nutrients, energy and temperature, and overlook the role of pore size. Our analyses consider sediment self-compaction, the evolution of pore size with depth, and the probabi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78714-3
更新日期：2020-12-10 00:00:00
abstract：:The competitive multi-armed bandit (CMAB) problem is related to social issues such as maximizing total social benefits while preserving equality among individuals by overcoming conflicts between individual decisions, which could seriously decrease social benefits. The study described herein provides experimental evide...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48647-7
更新日期：2019-08-22 00:00:00
abstract：:Voltage-gated potassium (Kv) channels comprise pore-forming α subunits and a multiplicity of regulatory proteins, including the cardiac-expressed and cardiac arrhythmia-linked transmembrane KCNE subunits. After recently uncovering novel, N-terminally extended (L) KCNE3 and KCNE4 isoforms and detecting their transcript...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38412
更新日期：2016-12-06 00:00:00
abstract：:Autophagy is a major regulator of pancreatic beta cell homeostasis. Altered autophagic activity has been implicated in the beta cells of patients with type 2 diabetes, and in the beta cells of obese diabetic rodents. Here, we show that autophagy was induced in beta cells by either a high-fat diet or a combined high-fa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16485-0
更新日期：2017-11-27 00:00:00
abstract：:We have prepared and tested a new surface enhanced Raman scattering (SERS) substrate based on self-assembled graphitic sheets to detect bisphenol A (BPA) in plastic consumer goods. Transmission electron microscopy (TEM) and atomic-force microscopy (AFM) were used to characterize the structure of the graphitic sheets a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17030-9
更新日期：2017-12-01 00:00:00
abstract：:The introduction of microCT has made it possible to show that the terminal bronchioles are narrowed and destroyed before the onset of emphysematous destruction in COPD. This report extends those observations to the cellular and molecular level in the centrilobular phenotype of emphysematous destruction in lungs donate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10126-2
更新日期：2017-08-25 00:00:00
abstract：:Widely regarded as an imminent threat to our oceans, ocean acidification has been documented in all oceanic basins. Projected changes in seawater chemistry will have catastrophic biotic effects due to ocean acidification hindering biogenic carbonate production, which will in turn lead to substantial changes in marine ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58501-w
更新日期：2020-01-31 00:00:00
abstract：:Milk is a major food of global economic importance, and its consumption is regarded as a classic example of gene-culture evolution. Humans have exploited animal milk as a food resource for at least 8500 years, but the origins, spread, and scale of dairying remain poorly understood. Indirect lines of evidence, such as ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07104
更新日期：2014-11-27 00:00:00
abstract：:We report a novel and facile method to synthesize sulfur-doped titanium oxide sheets and realize TiS2-TiO2 heterostructures by means of a sequential sulfurization and oxidation step in a dual-zone chemical vapor deposition furnace. The inclusion of chlorine and argon gases during the growth of such titanium-based comp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53651-y
更新日期：2019-11-29 00:00:00
abstract：:When coming to identify new 2D materials, our intuition would suggest us to look from layered instead of 3D materials. However, since graphite can be hypothetically derived from diamond by stretching it along its [111] axis, many 3D materials can also potentially be explored as new candidates for 2D materials. Using a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24660
更新日期：2016-04-19 00:00:00
abstract：:Micron-scale droplets isolated by an immiscible liquid can provide miniaturised reaction vessels which can be manipulated in microfluidic networks, and has seen a rapid growth in development. In many experiments, the precise volume of these microdroplets is a critical parameter which can be influenced by many external...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11254-5
更新日期：2017-09-05 00:00:00
abstract：:The study was aimed at assessing the diagnostic performance of 68Ga-PSMA-617 PET/CT in the detection of prostate cancer (PCa) in patients with a prostate-specific antigen (PSA) level of 4-20 ng/ml and to compare its efficacy with that of multiparametric MRI (mpMRI). We analyzed the data of 67 consecutive patients with...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67385-9
更新日期：2020-07-03 00:00:00
