Biosynthetic Routes for Producing Various Fucosyl-Oligosaccharides.

Abstract:

:Fucosyl-oligosaccharides (FOSs) play physiologically important roles as prebiotics, neuronal growth factors, and inhibitors of enteropathogens. However, challenges in designed synthesis and mass production of FOSs hamper their industrial applications. Here, we report flexible biosynthetic routes to produce various FOSs, including unnatural ones, through in vitro enzymatic reactions of various sugar acceptors, such as glucose, cellobiose, and agarobiose, and GDP-l-fucose as the fucose donor by using α1,2-fucosyltransferase (FucT2). Also, the whole-cell conversion for fucosylation of various sugar acceptors by overexpressing the genes associated with GDP-l-fucose production and fucT2 gene in Escherichia coli was demonstrated by producing 17.74 g/L of 2'-fucosylgalactose (2'-FG). Prebiotic effects of 2'-FG were verified on the basis of selective fermentability of 2'-FG by probiotic bifidobacteria. These biosynthetic routes can be used to engineer industrial microorganisms for more economical, more flexible, and safer production of FOSs than chemical synthesis of FOSs.

journal_name

ACS Synth Biol

journal_title

ACS synthetic biology

authors

Yun EJ,Liu JJ,Lee JW,Kwak S,Yu S,Kim KH,Jin YS

doi

10.1021/acssynbio.8b00436

subject

Has Abstract

pub_date

2019-02-15 00:00:00

pages

415-424

issue

2

issn

2161-5063

journal_volume

8

pub_type

杂志文章
  • Genetically Encoded Fluorescent Biosensor for Rapid Detection of Protein Expression.

    abstract::Fluorescent proteins are widely used as fusion tags to detect protein expression in vivo. To become fluorescent, these proteins must undergo chromophore maturation, a slow process with a half-time of 5 to >30 min that causes delays in real-time detection of protein expression. Here, we engineer a genetically encoded f...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.0c00407

    authors: Eason MG,Pandelieva AT,Mayer MM,Khan ST,Garcia HG,Chica RA

    更新日期:2020-11-20 00:00:00

  • In Vivo Gene Expression Dynamics of Tumor-Targeted Bacteria.

    abstract::The engineering of bacteria to controllably deliver therapeutics is an attractive application for synthetic biology. While most synthetic gene networks have been explored within microbes, there is a need for further characterization of in vivo circuit behavior in the context of applications where the host microbes are...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/sb3000639

    authors: Danino T,Lo J,Prindle A,Hasty J,Bhatia SN

    更新日期:2012-10-19 00:00:00

  • Evolutionary stability of a refactored phage genome.

    abstract::Engineered genetic systems are commonly unstable; if propagated, they evolve to reverse or modify engineered elements because the elements impair fitness. A goal of synthetic biology is thus to anticipate and avoid detrimental engineering, but little is yet known about which types of elements cause problems in differe...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/sb300040v

    authors: Springman R,Molineux IJ,Duong C,Bull RJ,Bull JJ

    更新日期:2012-09-21 00:00:00

  • A Novel Tool for Microbial Genome Editing Using the Restriction-Modification System.

    abstract::Scarless genetic manipulation of genomes is an essential tool for biological research. The restriction-modification (R-M) system is a defense system in bacteria that protects against invading genomes on the basis of its ability to distinguish foreign DNA from self DNA. Here, we designed an R-M system-mediated genome e...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.7b00254

    authors: Bai H,Deng A,Liu S,Cui D,Qiu Q,Wang L,Yang Z,Wu J,Shang X,Zhang Y,Wen T

    更新日期:2018-01-19 00:00:00

  • Biophysical, mutational, and functional investigation of the chromophore-binding pocket of light-oxygen-voltage photoreceptors.

    abstract::As light-regulated actuators, sensory photoreceptors underpin optogenetics and numerous applications in synthetic biology. Protein engineering has been applied to fine-tune the properties of photoreceptors and to generate novel actuators. For the blue-light-sensitive light-oxygen-voltage (LOV) photoreceptors, mutation...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/sb400205x

    authors: Diensthuber RP,Engelhard C,Lemke N,Gleichmann T,Ohlendorf R,Bittl R,Möglich A

    更新日期:2014-11-21 00:00:00

  • Bringing Light into Cell-Free Expression.

    abstract::Cell-free systems, as part of the synthetic biology field, have become a critical platform in biological studies. However, there is a lack of research into developing a switch for a dynamical control of the transcriptional and translational process. The optogenetic tool has been widely proven as an ideal control switc...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.0c00211

    authors: Zhang P,Yang J,Cho E,Lu Y

    更新日期:2020-08-21 00:00:00

  • Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli.

    abstract::Although Escherichia coli has been a popular tool for plasmid construction, this bacterium was believed to be "unsuitable" for constructing a large plasmid whose size exceeds 500 kilobases. We assumed that traditional plasmid vectors may lack some regulatory DNA elements required for the stable replication and segrega...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.0c00008

    authors: Mukai T,Yoneji T,Yamada K,Fujita H,Nara S,Su'etsugu M

    更新日期:2020-06-19 00:00:00

  • Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1.

    abstract::Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different ...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/sb3000244

    authors: Zhang D,Zhao Y,He Y,Wang Y,Zhao Y,Zheng Y,Wei X,Zhang L,Li Y,Jin T,Wu L,Wang H,Davison PA,Xu J,Huang WE

    更新日期:2012-07-20 00:00:00

  • An Engineered Survival-Selection Assay for Extracellular Protein Expression Uncovers Hypersecretory Phenotypes in Escherichia coli.

    abstract::The extracellular expression of recombinant proteins using laboratory strains of Escherichia coli is now routinely achieved using naturally secreted substrates, such as YebF or the osmotically inducible protein Y (OsmY), as carrier molecules. However, secretion efficiency through these pathways needs to be improved fo...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.6b00366

    authors: Natarajan A,Haitjema CH,Lee R,Boock JT,DeLisa MP

    更新日期:2017-05-19 00:00:00

  • New Prodigiosin Derivatives Obtained by Mutasynthesis in Pseudomonas putida.

    abstract::The deeply red-colored natural compound prodigiosin is a representative of the prodiginine alkaloid family, which possesses bioactivities as antimicrobial, antitumor, and antimalarial agents. Various bacteria including the opportunistic human pathogen Serratia marcescens and different members of the Streptomycetaceae ...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.7b00099

    authors: Klein AS,Domröse A,Bongen P,Brass HUC,Classen T,Loeschcke A,Drepper T,Laraia L,Sievers S,Jaeger KE,Pietruszka J

    更新日期:2017-09-15 00:00:00

  • An Orthogonal Multi-input Integration System to Control Gene Expression in Escherichia coli.

    abstract::In many biotechnological applications, it is useful for gene expression to be regulated by multiple signals, as this allows the programming of complex behavior. Here we implement, in Escherichia coli, a system that compares the concentration of two signal molecules, and tunes GFP expression proportionally to their rel...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.7b00109

    authors: Annunziata F,Matyjaszkiewicz A,Fiore G,Grierson CS,Marucci L,di Bernardo M,Savery NJ

    更新日期:2017-10-20 00:00:00

  • GeneORator: An Effective Strategy for Navigating Protein Sequence Space More Efficiently through Boolean OR-Type DNA Libraries.

    abstract::Directed evolution requires the creation of genetic diversity and subsequent screening or selection for improved variants. For DNA mutagenesis, conventional site-directed methods implicitly utilize the Boolean AND operator (creating all mutations simultaneously), producing a combinatorial explosion in the number of ge...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.9b00063

    authors: Currin A,Kwok J,Sadler JC,Bell EL,Swainston N,Ababi M,Day P,Turner NJ,Kell DB

    更新日期:2019-06-21 00:00:00

  • Metabolic Engineering of Pseudomonas putida KT2440 for Complete Mineralization of Methyl Parathion and γ-Hexachlorocyclohexane.

    abstract::Agricultural soils are often cocontaminated with multiple pesticides. Unfortunately, microorganisms isolated from natural environments do not possess the ability to simultaneously degrade different classes of pesticides. Currently, we can use the approaches of synthetic biology to create a strain endowed with various ...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.6b00025

    authors: Gong T,Liu R,Zuo Z,Che Y,Yu H,Song C,Yang C

    更新日期:2016-05-20 00:00:00

  • Programming the Dynamic Control of Bacterial Gene Expression with a Chimeric Ligand- and Light-Based Promoter System.

    abstract::To program cells in a dynamic manner, synthetic biologists require precise control over the threshold levels and timing of gene expression. However, in practice, modulating gene expression is widely carried out using prototypical ligand-inducible promoters, which have limited tunability and spatiotemporal resolution. ...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.8b00280

    authors: Jayaraman P,Yeoh JW,Zhang J,Poh CL

    更新日期:2018-11-16 00:00:00

  • Development of High-Performance Whole Cell Biosensors Aided by Statistical Modeling.

    abstract::Whole cell biosensors are genetic systems that link the presence of a chemical, or other stimulus, to a user-defined gene expression output for applications in sensing and control. However, the gene expression level of biosensor regulatory components required for optimal performance is nonintuitive, and classical iter...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.9b00448

    authors: Berepiki A,Kent R,Machado LFM,Dixon N

    更新日期:2020-03-20 00:00:00

  • Robust Characterization of Two Distinct Glutarate Sensing Transcription Factors of Pseudomonas putida l-Lysine Metabolism.

    abstract::A significant bottleneck in synthetic biology involves screening large genetically encoded libraries for desirable phenotypes such as chemical production. However, transcription factor-based biosensors can be leveraged to screen thousands of genetic designs for optimal chemical production in engineered microbes. In th...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.9b00255

    authors: Thompson MG,Costello Z,Hummel NFC,Cruz-Morales P,Blake-Hedges JM,Krishna RN,Skyrud W,Pearson AN,Incha MR,Shih PM,Garcia-Martin H,Keasling JD

    更新日期:2019-10-18 00:00:00

  • Cooperativity to increase Turing pattern space for synthetic biology.

    abstract::It is hard to bridge the gap between mathematical formulations and biological implementations of Turing patterns, yet this is necessary for both understanding and engineering these networks with synthetic biology approaches. Here, we model a reaction-diffusion system with two morphogens in a monostable regime, inspire...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/sb500233u

    authors: Diambra L,Senthivel VR,Menendez DB,Isalan M

    更新日期:2015-02-20 00:00:00

  • MEGA (Multiple Essential Genes Assembling) deletion and replacement method for genome reduction in Escherichia coli.

    abstract::Top-down reduction of the bacterial genome to construct desired chassis cells is important for synthetic biology. However, the current progress in the field of genome reduction is greatly hindered by indispensable life-essential genes that are interspersed throughout the chromosomal loci. Here, we described a new meth...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/sb500324p

    authors: Xue X,Wang T,Jiang P,Shao Y,Zhou M,Zhong L,Wu R,Zhou J,Xia H,Zhao G,Qin Z

    更新日期:2015-06-19 00:00:00

  • Active Analyte Import Improves the Dynamic Range and Sensitivity of a Vitamin B12 Biosensor.

    abstract::Cell-free systems provide a versatile platform for the development of low-cost, easy-to-use sensors for diverse analytes. However, sensor affinity dictates response sensitivity, and improving binding affinity can be challenging. Here, we describe efforts to address this problem while developing a biosensor for vitamin...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.9b00429

    authors: McNerney MP,Piorino F,Michel CL,Styczynski MP

    更新日期:2020-02-21 00:00:00

  • Toward Synthetic Spatial Patterns in Engineered Cell Populations with Chemotaxis.

    abstract::A major force shaping form and patterns in biology is based in the presence of amplification mechanisms able to generate ordered, large-scale spatial structures out of local interactions and random initial conditions. Turing patterns are one of the best known candidates for such ordering dynamics, and their existence ...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.5b00254

    authors: Duran-Nebreda S,Solé RV

    更新日期:2016-07-15 00:00:00

  • The Experiment Data Depot: A Web-Based Software Tool for Biological Experimental Data Storage, Sharing, and Visualization.

    abstract::Although recent advances in synthetic biology allow us to produce biological designs more efficiently than ever, our ability to predict the end result of these designs is still nascent. Predictive models require large amounts of high-quality data to be parametrized and tested, which are not generally available. Here, ...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.7b00204

    authors: Morrell WC,Birkel GW,Forrer M,Lopez T,Backman TWH,Dussault M,Petzold CJ,Baidoo EEK,Costello Z,Ando D,Alonso-Gutierrez J,George KW,Mukhopadhyay A,Vaino I,Keasling JD,Adams PD,Hillson NJ,Garcia Martin H

    更新日期:2017-12-15 00:00:00

  • Design and Characterization of an Icosahedral Protein Cage Formed by a Double-Fusion Protein Containing Three Distinct Symmetry Elements.

    abstract::Exploiting simple types of symmetry common to many natural protein oligomers as a starting point, several recent studies have succeeded in engineering complex self-assembling protein architectures reminiscent but distinct from those evolved in the natural world. Designing symmetric protein cages with a wide range of p...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.9b00392

    authors: Cannon KA,Nguyen VN,Morgan C,Yeates TO

    更新日期:2020-03-20 00:00:00

  • Exploring the heterologous genomic space for building, stepwise, complex, multicomponent tolerance to toxic chemicals.

    abstract::Modern bioprocessing depends on superior cellular traits, many stemming from unknown genes and gene interactions. Tolerance to toxic chemicals is such an industrially important complex trait, which frequently limits the economic feasibility of producing commodity chemicals and biofuels. Chemical tolerance encompasses ...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/sb400156v

    authors: Zingaro KA,Nicolaou SA,Yuan Y,Papoutsakis ET

    更新日期:2014-07-18 00:00:00

  • Biosynthesis of antimycins with a reconstituted 3-formamidosalicylate pharmacophore in Escherichia coli.

    abstract::Antimycins are a family of natural products generated from a hybrid nonribosomal peptide synthetase (NRPS)-polyketide synthase (PKS) assembly line. Although they possess an array of useful biological activities, their structural complexity makes chemical synthesis challenging, and their biosynthesis has thus far been ...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/sb5003136

    authors: Liu J,Zhu X,Seipke RF,Zhang W

    更新日期:2015-05-15 00:00:00

  • A Modular Receptor Platform To Expand the Sensing Repertoire of Bacteria.

    abstract::Engineered bacteria promise to revolutionize diagnostics and therapeutics, yet many applications are precluded by the limited number of detectable signals. Here we present a general framework to engineer synthetic receptors enabling bacterial cells to respond to novel ligands. These receptors are activated via ligand-...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.7b00266

    authors: Chang HJ,Mayonove P,Zavala A,De Visch A,Minard P,Cohen-Gonsaud M,Bonnet J

    更新日期:2018-01-19 00:00:00

  • Engineered Photoactivatable Genetic Switches Based on the Bacterium Phage T7 RNA Polymerase.

    abstract::Genetic switches in which the activity of T7 RNA polymerase (RNAP) is directly regulated by external signals are obtained with an engineering strategy of splitting the protein into fragments and using regulatory domains to modulate their reconstitutions. Robust switchable systems with excellent dark-off/light-on prope...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.6b00248

    authors: Han T,Chen Q,Liu H

    更新日期:2017-02-17 00:00:00

  • Labeling RNAs in Live Cells Using Malachite Green Aptamer Scaffolds as Fluorescent Probes.

    abstract::RNAs mediate many different processes that are central to cellular function. The ability to quantify or image RNAs in live cells is very useful in elucidating such functions of RNA. RNA aptamer-fluorogen systems have been increasingly used in labeling RNAs in live cells. Here, we use the malachite green aptamer (MGA),...

    journal_title:ACS synthetic biology

    pub_type: 信件

    doi:10.1021/acssynbio.7b00237

    authors: Yerramilli VS,Kim KH

    更新日期:2018-03-16 00:00:00

  • A tetO Toolkit To Alter Expression of Genes in Saccharomyces cerevisiae.

    abstract::Strategies to optimize a metabolic pathway often involve building a large collection of strains, each containing different versions of sequences that regulate the expression of pathway genes. Here, we develop reagents and methods to carry out this process at high efficiency in the yeast Saccharomyces cerevisiae. We id...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/sb500363y

    authors: Cuperus JT,Lo RS,Shumaker L,Proctor J,Fields S

    更新日期:2015-07-17 00:00:00

  • Synthesis and Characterization of Heterodimers and Fluorescent Nisin Species by Incorporation of Methionine Analogues and Subsequent Click Chemistry.

    abstract::Noncanonical amino acids form a highly diverse pool of building blocks that can render unique physicochemical properties to peptides and proteins. Here, four methionine analogues with unsaturated and varying side chain lengths were successfully incorporated at four different positions in nisin in Lactococcus lactis th...

    journal_title:ACS synthetic biology

    pub_type: 杂志文章

    doi:10.1021/acssynbio.0c00308

    authors: Deng J,Viel JH,Chen J,Kuipers OP

    更新日期:2020-09-18 00:00:00

  • Gene-Mediated Chemical Communication in Synthetic Protocell Communities.

    abstract::A gene-directed chemical communication pathway between synthetic protocell signaling transmitters (lipid vesicles) and receivers (proteinosomes) was designed, built and tested using a bottom-up modular approach comprising small molecule transcriptional control, cell-free gene expression, porin-directed efflux, substra...

    journal_title:ACS synthetic biology

    pub_type: 信件

    doi:10.1021/acssynbio.7b00306

    authors: Tang TD,Cecchi D,Fracasso G,Accardi D,Coutable-Pennarun A,Mansy SS,Perriman AW,Anderson JLR,Mann S

    更新日期:2018-02-16 00:00:00