Abstract:
OBJECTIVES:Non-albicans Candida (NAC) species are increasingly identified as pathogens causing oral candidiasis. Incidence rates for azole resistance among NAC species have been continuously reported. This study aimed to evaluate the azole susceptibility profiles and to characterise the azole resistance mechanisms of oral clinical NAC isolates. METHODS:In vitro susceptibility patterns of 85 NAC species isolates were determined by the broth microdilution method. Azole resistance-related genes (ERG3, ERG11 and PDR1) of Candida glabrata isolates were sequenced to determine the presence of nucleotide substitutions. Expression levels of various resistance-related genes were also evaluated by RT-qPCR in azole-susceptible, susceptible dose-dependent (SDD) and resistant Candida isolates. RESULTS:Two C. glabrata isolates (2.4% of all NAC isolates) were resistant to all three azoles tested (fluconazole, itraconazole and ketoconazole). All clinical isolates of Candida tropicalis and Candida kefyr were susceptible to azoles. Silent mutations were found in the CgERG11 and CgERG3 genes of clinical C. glabrata isolates. Interestingly, two missense mutations in CgPDR1 (N768D and E818K) were identified only in resistant C. glabrata isolates. The presence of a CgPDR1 missense mutation in resistant isolates is associated with overexpression of its own product as well as multidrug transporters including ABC and MFS transporters. CONCLUSION:A gain-of-function (GOF) mutation in CgPDR1 is associated with upregulation of various drug transporters, which appears to serve as a primary mechanism for azole resistance in the detected C. glabrata isolates. Therefore, analysis of GOF mutations in the PDR1 regulator provides a better understanding of the development of antifungal resistance.
journal_name
J Glob Antimicrob Resistjournal_title
Journal of global antimicrobial resistanceauthors
Tantivitayakul P,Lapirattanakul J,Kaypetch R,Muadcheingka Tdoi
10.1016/j.jgar.2019.01.006subject
Has Abstractpub_date
2019-06-01 00:00:00pages
221-226eissn
2213-7165issn
2213-7173pii
S2213-7165(19)30013-Xjournal_volume
17pub_type
杂志文章abstract::Mycobacterium tuberculosis is naturally resistant to clarithromycin (CLR). The genes Rv3197A (whiB7) and Rv1988 (ermMT) have been shown to be involved in the resistant phenotype. In this study, a CLR-susceptible M. tuberculosis clinical strain was identified, designated as DS3214, and the nucleotide sequences and expr...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2015.07.004
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVES:Staphylococcus cohnii has become increasingly recognized as a potential pathogen of clinically significant nosocomial and farm animal infections. This study was designed to determine the genome of a multidrug-resistant S. cohnii subsp. urealyticus strain SNUDS-2 isolated from a farmed duck in Korea. METHODS...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.05.008
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:Delafloxacin is a broad-spectrum anionic fluoroquinolone with activity against Gram-positive and Gram-negative organisms, including methicillin-resistant Staphylococcus aureus. Both oral and intravenous formulations were approved for use in the treatment of acute bacterial skin and skin-structure infections ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.09.027
更新日期:2020-12-01 00:00:00
abstract::Acinetobacter baumannii coccobacilli are dangerous to patients in intensive care units because of their multidrug resistance to antibiotics, developed mainly in the past decade. This study aimed to examine whether there is a significant correlation between the number of Pro-Ala repeats in the CAP01997 protein, the Emr...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.09.004
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:The objective was to investigate how deficient of quorum sensing system (QS) affects minimal inhibitory concentrations (MICs) of Pseudomonas aeruginosa strains to ciprofloxacin (CIP) and levofloxacin (LEV) in vitro. METHODS:Collected the clinical Pseudomonas aeruginosa isolates. Screened the strains deficie...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.04.008
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:Carbapenem-resistant Acinetobacter baumannii (CRAB) have emerged as a serious threat to public-health worldwide. This study aimed to determine the antimicrobial susceptibility of A. baumannii isolates in Iran and to investigate oxacillinase-encoding determinants and their association with insertion sequence ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.12.011
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:Multidrug-resistant (MDR) enterococci are found extensively in food samples. This study characterized the phenotypic virulence factors and the ability of horizontal gene transfer of a streptomycin resistance gene among enterococci isolated from fermented pork. METHODS:Thirty-six MDR enterococci were subject...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.04.016
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:To determine the contribution of specific uropathogenic Escherichia coli (UPEC) lineages, drug resistance genes, and plasmid incompatibility/replicon (Inc) groups to the prevalence of β-lactam-resistant urinary tract infections (UTIs) in a university community. METHODS:Urine samples were consecutively colle...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.03.002
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVES:The widespread dissemination of extended-spectrum β-lactamase-producing Enterobacteriaceae has become a major issue in veterinary medicine. However, until now, there has been no report of bacteria with such a phenotype in infected snakes. The aim of this study was to report the first draft genome sequence of...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.11.009
更新日期:2018-12-01 00:00:00
abstract::Acinetobacter calcoaceticus-baumannii complex is a common cause of hospital-acquired infections (HAIs) globally, remarkable for its high rate of antibiotic resistance, including to carbapenems. There are few data on the resistance of A. baumannii in Vietnam, which are essential for developing evidence-based treatment ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2014.05.003
更新日期:2014-12-01 00:00:00
abstract::The genus Kluyvera comprises potential pathogens that can cause many infections. This study reports a Kluyvera intermedia strain (FOSA7093) from a pancreatic cyst specimen from a long-term hospitalised patient. Whole-genome sequencing (WGS) of the K. intermedia isolate was performed and the strain was reported as sens...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.05.007
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to validate the use of LML antimicrobial gradient strips for quantitative determination of carbapenem susceptibility in Enterobacteriaceae. METHODS:A total of 95 non-redundant Enterobacteriaceae strains isolated during 2012-2014 were used for this validation study. Initially, LML a...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2016.10.010
更新日期:2017-03-01 00:00:00
abstract::β-Lactams are a commonly used class of bactericidal antibiotics. The number of β-lactam-resistant pathogens is constantly increasing in hospitals around the world. Interestingly, most of the β-lactam-resistant bacteria carry mobile genetic elements, such as conjugative plasmids, that render the pathogen resistant. The...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2014.02.005
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVES:Infections caused by multidrug-resistant Gram-negative bacteria are associated with high mortality. A relevant concern is the efficacy of antibiotic therapy in burn patients in whom pathophysiological changes strongly influence pharmacokinetic (PK) parameters. This study aimed to describe the PK parameters o...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.06.011
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:Flies have been implicated in the dispersal of medically important bacteria including members of the genus Klebsiella between different environmental compartments. The aim of this study was to retrieve and characterize antibiotic-resistant bacteria from flies collected near to hospitals. METHODS:Flies were ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.11.021
更新日期:2020-12-07 00:00:00
abstract:OBJECTIVE:Little is known about the treatment outcomes of HIV-negative drug-resistant tuberculosis (TB) patients in Malaysia. With respect to this issue, this study aimed to determine factors associated with unsuccessful treatment outcomes among drug-resistant TB patients at the Institute of Respiratory Medicine, Kuala...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.05.009
更新日期:2019-12-01 00:00:00
abstract::Environmental fungicides are used in agriculture to reduce fungal spoilage of crops to a minimum, and the polyene macrolide natamycin is used as a food preservative. The use of natamycin in yoghurt has recently been authorised in the USA and some other countries. However, resistance development is a serious risk assoc...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章,评审
doi:10.1016/j.jgar.2017.10.024
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to examine the performance of the chromogenic β LACTA™ test for the rapid detection of expanded-spectrum cephalosporin (ESC) non-susceptibility among Enterobacteriaceae in a region endemic for potent β-lactamases. METHODS:The β LACTA™ test was applied prospectively on 235 consecuti...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.05.018
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine the percentage of antimicrobial-resistant isolates and the associated resistance mechanisms in Gram-negative bacteria from South Western Nigeria. METHODS:A total of 306 non-duplicate unbiased Gram-negative isolates were recovered from patients admitted to three teachin...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.10.016
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVES:Here we describe a clinical isolate of Klebsiella pneumoniae ST11 harbouring both blaKPC-2 and rmtB genes in Japan. METHODS:A carbapenem- and aminoglycoside-resistant K. pneumoniae was isolated from an inpatient in Japan. Whole-genome sequencing (WGS) was performed using an Illumina next-generation sequence...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2018.11.026
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVES:Citrobacter spp. are part of normal human and animal intestinal flora. Citrobacter portucalensis (C. portucalensis) is closely related to Citrobacter freundii, which is an emerging opportunistic nosocomial pathogen. The aim of this study was to retrieve colistin-resistant Citrobacter spp. from poultry in Ban...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.05.031
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate the efficacy of pyrimethamine-loaded poloxamer 407 nanomicelles on Plasmodium berghei strain NICD in vivo. METHODS:Pyrimethamine-loaded nanomicelles were prepared and their zeta potential, particle size and polydispersity index were measured. For antiplasmodial assessme...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.08.002
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine the prevalence of nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among healthcare workers (HCWs) compared with non-HCWs at Duhok city, Kurdistan Region, northern Iraq. METHODS:A total of 182 HCWs with different occupations and working in different...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2017.01.010
更新日期:2017-06-01 00:00:00
abstract:OBJECTIVES:The emergence of multidrug-resistance (MDR) in Streptococcus pneumoniae clones and non-vaccine serotypes necessitate the development of novel treatment strategies. This work aimed to determine the efficacy of the Mn complex [Mn(CO)3(tpa-κ3N)]Br against clinically important MDR strains of S. pneumoniae. METH...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.04.031
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to forecast the monthly incidence rates of infections [infections/1000 bed-days (IBD)] due to carbapenem-resistant Klebsiella pneumoniae (CRKP), carbapenem-resistant Pseudomonas aeruginosa (CRPA), carbapenem-resistant Acinetobacter baumannii (CRAB) and total carbapenem-resistant Gra...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.06.019
更新日期:2020-03-01 00:00:00
abstract::Although molecular methods can now provide fast identification and the antibiotic susceptibility profile of infecting pathogens, these techniques are not affordable by a large majority of clinical laboratories in developing countries and can be considered excessive for simple, community-acquired infections. Most antib...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2013.01.001
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVES:Recently, a growing resistance to antimalarial drugs such as chloroquine, sulfadoxine-pyrimethamine, artemisinin derivatives and mefloquine has been observed. The pharmacokinetic limitation of the current therapy and multi-drug resistance has resulted in an urgent need to study the new antimalarial combinati...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2020.02.018
更新日期:2020-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to define the relationship between age and response to tigecycline among patients treated for complicated skin and soft-tissue infections (cSSTIs) and complicated intra-abdominal infections (cIAIs). METHODS:Pooled data derived from five European observational studies on the use of ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.03.018
更新日期:2019-09-01 00:00:00
abstract::In the past few years, Europe has experienced an enormous influx of refugees and migrants owing to the ongoing civil war in Syria as well as conflicts, violence and instability in other Asian and African countries. Available data suggest that refugees carry a significant burden of multidrug-resistant (MDR) organisms, ...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章,评审
doi:10.1016/j.jgar.2017.03.013
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:The first report of a plasmid-borne colistin resistance gene (mcr-1) detected in an Escherichia coli isolate from China heralded the emergence of pandrug-resistant bacteria. Since then, the mcr-1 gene has been detected worldwide, but to date it has not been reported in the Philippines. METHODS:In this study...
journal_title:Journal of global antimicrobial resistance
pub_type: 杂志文章
doi:10.1016/j.jgar.2019.12.018
更新日期:2020-06-01 00:00:00