c-Src kinase contributes on endothelial cells mechanotransduction in a heat shock protein 70-dependent turnover manner.

Abstract:

:Shear stress changes are associated with a repertory of signaling cascade modulating vascular phenotype. As shear stress-related tensional forces might be associated with pathophysiological susceptibility, a more comprehensive molecular map needs to be addressed. Thus, we subjected human umbilical vein endothelial cells (HUVECs) to a circuit of different tensional forces in vitro considering the following three groups: (a) physiological blood flow shear stress condition (named Normo), (b) a hypertensive blood flow shear stress (named Hyper), and (c) these hyper-stressed cells were returned to Normo condition (named Return). The samples were properly collected to allow different methodologies analysis. Our data showed a pivotal involvement of c-Src on driving the mechanotransduction cascade by modulating signaling related with adhesion, survival (PI3K/Akt) and proliferative phenotype. Moreover, c-Src seems to develop important role during extracellular matrix remodeling. Additionally, proteomic analysis showed strong involvement of heat shock protein 70 (HSP70) in the hypertensive-stressed cells; it being significantly decreased in return phenotype. This result prompted us to investigate 20S proteasome as an intracellular proteolytic alternative route to promote the turnover of those proteins. Surprisingly, our data reveled significant overexpression of sets of proteasome subunit α-type (PSMA) and β-type (PSMB) genes. In conjunction, our data showed c-Src as a pivotal protein to drive mechanotransduction in endothelial cells in a HSP70-dependent turnover scenario. Because shear patterns is associated with pathophysiological changes, such as atherosclerosis and hypertension, these results paved new road to understand the molecular mechanism on driving mechanotransduction in endothelial cells and, if drugable, these targets must be considered within pharmacological treatment optimization.

journal_name

J Cell Physiol

authors

Pinto TS,Fernandes CJDC,da Silva RA,Gomes AM,Vieira JCS,Padilha PM,Zambuzzi WF

doi

10.1002/jcp.27787

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

11287-11303

issue

7

eissn

0021-9541

issn

1097-4652

journal_volume

234

pub_type

杂志文章
  • Cell cycle-related changes in regulatory volume decrease and volume-sensitive chloride conductance in mouse fibroblasts.

    abstract::Cell cycle-related changes in the ability to regulate cell volume following hyposmotic swelling were studied in mouse fibroblasts using videomicroscopy and the whole-cell patch clamp technique. Regulatory volume decrease (RVD) and volume-sensitive Cl- conductance (G(Cl,vol)) were measured: (1) in proliferating cells o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/1097-4652(200104)187:1<65::AID-JCP1052>3.0

    authors: Doroshenko P,Sabanov V,Doroshenko N

    更新日期:2001-04-01 00:00:00

  • Autophagy, anoikis, ferroptosis, necroptosis, and endoplasmic reticulum stress: Potential applications in melanoma therapy.

    abstract::Melanoma as the most major skin malignancy has attracted much attention, so far. Although a successful therapeutic strategy requires an accurate understanding of the precise mechanisms for the initiation and progression of the melanoma. Several types of cell death mechanisms have recently been identified along with co...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28740

    authors: Ashrafizadeh M,Mohammadinejad R,Tavakol S,Ahmadi Z,Roomiani S,Katebi M

    更新日期:2019-11-01 00:00:00

  • 16,16-Dimethyl Prostaglandin E2 modulation of endothelial monolayer paracellular barrier function.

    abstract::Prostaglandins prevent gastrointestinal mucosal injury and promote healing following mucosal injury by various noxious agents. Preservation or repair of microvascular function appears to be crucial in these processes. The processes involved in prostaglandin-mediated repair and preservation of endothelial function are ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199605)167:2<204::AID-JCP3

    authors: Ma TY,Pedram A

    更新日期:1996-05-01 00:00:00

  • Reduction of DNA primase activity in aging but still proliferating cells.

    abstract::The basis of the well-known decline in cell proliferation with increasing passage number of human diploid fibroblast-like cell cultures is not known. It has been found that DNA synthesis was deficient in the remaining but still proliferating cells, but when appropriate corrections reflecting the remaining dividing cel...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041430107

    authors: Collins JM,Chu AK

    更新日期:1990-04-01 00:00:00

  • Defect in polyamine metabolism in a BHK cell mutant temperature-sensitive for rRNA maturation.

    abstract::A mutant of BHK cells (ts422E) temperature-sensitive for processing 32S rRNA to 28S rRNA (Toniolo et al., '73) also loses the ability to synthesize polyamines and 5.8S rRNA when shifted to the non-permissive temperature (39 degrees). The activity of several enzymes not involved with polyamine synthesis, methylation of...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041010303

    authors: Levin EG,Clark JL

    更新日期:1979-12-01 00:00:00

  • Mll-COMPASS complexes mediate H3K4me3 enrichment and transcription of the osteoblast master gene Runx2/p57 in osteoblasts.

    abstract::Expression of Runx2/p57 is a hallmark of the osteoblast-lineage identity. Although several regulators that control the expression of Runx2/p57 during osteoblast-lineage commitment have been identified, the epigenetic mechanisms that sustain this expression in differentiated osteoblasts remain to be completely determin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27355

    authors: Rojas A,Sepulveda H,Henriquez B,Aguilar R,Opazo T,Nardocci G,Bustos F,Lian JB,Stein JL,Stein GS,van Zundert B,van Wijnen AJ,Allende ML,Montecino M

    更新日期:2019-05-01 00:00:00

  • Electrophysiological characterization of voltage-gated Na+ current expressed in the highly metastatic Mat-LyLu cell line of rat prostate cancer.

    abstract::Voltage-gated Na+ channels, classically associated with impulse conduction in excitable tissues, are also found in a variety of epithelial cell types where their possible functions are not known so well. We have previously reported expression of a voltage-gated Na+ channel specifically in the highly metastatic Mat-LyL...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199804)175:1<50::AID-JCP6>

    authors: Grimes JA,Djamgoz MB

    更新日期:1998-04-01 00:00:00

  • Activated K-Ras and INK4a/Arf deficiency promote aggressiveness of pancreatic cancer by induction of EMT consistent with cancer stem cell phenotype.

    abstract::Pancreatic ductal adenocarcinoma (PDAC) is one of the most frequently diagnosed cancers and the fourth leading cause of cancer-related death in the United States, suggesting that there is an urgent need to design novel strategies for achieving better treatment outcome of patients diagnosed with PDAC. Our previous stud...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,收录出版

    doi:10.1002/jcp.24162

    authors: Wang Z,Ali S,Banerjee S,Bao B,Li Y,Azmi AS,Korc M,Sarkar FH

    更新日期:2013-03-01 00:00:00

  • Induction of autocrine epidermal growth factor receptor ligands in human keratinocytes by insulin/insulin-like growth factor-1.

    abstract::Autocrine activation of the epidermal growth factor (EGF) receptor on keratinocytes has been recognized as an important growth regulatory mechanism involved in epithelial homeostasis, and, possibly, hyperproliferative diseases. Insulin-like growth factor (IGF)-1 and insulin have been shown to be paracrine keratinocyte...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041630206

    authors: Vardy DA,Kari C,Lazarus GS,Jensen PJ,Zilberstein A,Plowman GD,Rodeck U

    更新日期:1995-05-01 00:00:00

  • Cell-cycle-dependent turnover of P-glycoprotein in multidrug-resistant cells.

    abstract::Regulation of P-glycoprotein (Pgp) expression occurs not only at the DNA and mRNA level but also at the protein level. We showed previously that Pgp was stabilized when multidrug-resistant CH(R)C5 and SKVCR 2.0 ovarian cell lines were subjected to serum-starved or high-cell-density growth conditions, whereas Pgp turno...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(200007)184:1<17::AID-JCP2>

    authors: Zhang W,Ling V

    更新日期:2000-07-01 00:00:00

  • Genetic Architecture of Sexual Dimorphism in Humans.

    abstract::Males and females differ across a broad spectrum of morphological, physiological, and behavioral characters. In fact, sexually dimorphic traits typically contribute the largest component of phenotypic variance in most taxa that use sex to reproduce. However, we know very little about the mechanisms that maintain these...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.24979

    authors: Rigby N,Kulathinal RJ

    更新日期:2015-10-01 00:00:00

  • PACAP and PAC1R are differentially expressed in motor cortex of amyotrophic lateral sclerosis patients and support survival of iPSC-derived motor neurons.

    abstract::Amyotrophic lateral sclerosis (ALS) is a fatal and disabling neurodegenerative disease characterized by upper and lower motor neurons depletion. In our previous work, comprehensive genomic profiling of 41 motor cortex samples enabled to discriminate controls from sporadic ALS patients, and segregated these latter into...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26182

    authors: Bonaventura G,Iemmolo R,D'Amico AG,La Cognata V,Costanzo E,Zappia M,D'Agata V,Conforti FL,Aronica E,Cavallaro S

    更新日期:2018-04-01 00:00:00

  • LncRNA NEAT1 promotes extracellular matrix accumulation and epithelial-to-mesenchymal transition by targeting miR-27b-3p and ZEB1 in diabetic nephropathy.

    abstract::Diabetic nephropathy (DN) is a kind of microvascular complications of diabetes. Long noncoding RNAs (lnRNAs) can participate in the development of various diseases, including DN. However, the function of lncRNA NEAT1 is unclear. In our present study, we reported that NEAT1 was significantly increased in streptozotocin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27959

    authors: Wang X,Xu Y,Zhu YC,Wang YK,Li J,Li XY,Ji T,Bai SJ

    更新日期:2019-08-01 00:00:00

  • Premature chromosome condensation and cell cycle analysis.

    abstract::The application of the phenomenon of premature chromosome condensation for cell cycle analysis in HeLa and CHO cells has been examined. Random populations of HeLa and CHO cells pulse labelled with H3-TdR were separately fused with mitotic HeLa cells using U.V. inactivated Sendai virus. The resulting prematurely conden...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040910113

    authors: Rao PN,Wilson B,Puck TT

    更新日期:1977-04-01 00:00:00

  • Increased peptidylarginine deiminase expression during induction of prolactin biosynthesis in a growth-hormone-producing rat pituitary cell line, MtT/S.

    abstract::Insulin and type I insulin-like growth factor (IGF-I) suppressed growth hormone (GH) expression followed by the induction of prolactin (PRL) biosynthesis in MtT/S cells cultured with normal sera. Insulin also increased the peptidylarginine deiminase activity in a dose-dependent manner. The increase was detectable at 1...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041500227

    authors: Nagata S,Uehara T,Inoue K,Senshu T

    更新日期:1992-02-01 00:00:00

  • Desferoxamine protects against glucocorticoid-induced osteonecrosis of the femoral head via activating HIF-1α expression.

    abstract::Glucocorticoid-induced osteonecrosis of the femoral head (GIOFH) is one of the most common complications of glucocorticoid administration. By chelating Fe2+ , desferoxamine (DFO) was reported to be able to activate the HIF-1α/VEGF pathway and promote angiogenesis. In the present study, we examined whether DFO administ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29799

    authors: Jing X,Du T,Yang X,Zhang W,Wang G,Liu X,Li T,Jiang Z

    更新日期:2020-12-01 00:00:00

  • Flt-1, but not Flk-1 mediates hyperpermeability through activation of the PI3-K/Akt pathway.

    abstract::Vascular endothelial growth factor (VEGF), a potent mediator of endothelial proliferation and migration, has an important role also in brain edema formation during hypoxia and ischemia. VEGF binds to the tyrosine kinase receptors Flt-1 and Flk-1. Yet, their relative importance for hypoxia-induced hyperpermeability is ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21022

    authors: Vogel C,Bauer A,Wiesnet M,Preissner KT,Schaper W,Marti HH,Fischer S

    更新日期:2007-07-01 00:00:00

  • Global analysis of gene expression profiles in the submandibular salivary gland of klotho knockout mice.

    abstract::Salivary dysfunction commonly occurs in many older adults and is considered a physiological phenomenon. However, the genetic changes in salivary glands during aging have not been characterized. The present study analyzed the gene expression profile in salivary glands from accelerated aging klotho deficient mice (kloth...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26172

    authors: Kwon SM,Kim SA,Yoon JH,Yook JI,Ahn SG

    更新日期:2018-04-01 00:00:00

  • BMP-3 promotes mesenchymal stem cell proliferation through the TGF-beta/activin signaling pathway.

    abstract::Adipogenesis plays a key role in the pathogenesis of obesity. It begins with the commitment of mesenchymal stem cells (MSCs) to the adipocyte lineage, followed by terminal differentiation of preadipocytes to mature adipocytes. A critical, but poorly understood, component of adipogenesis involves proliferation of MSCs ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22064

    authors: Stewart A,Guan H,Yang K

    更新日期:2010-06-01 00:00:00

  • Microbial Community Metabolic Modeling: A Community Data-Driven Network Reconstruction.

    abstract::Metabolic network modeling of microbial communities provides an in-depth understanding of community-wide metabolic and regulatory processes. Compared to single organism analyses, community metabolic network modeling is more complex because it needs to account for interspecies interactions. To date, most approaches foc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25428

    authors: Henry CS,Bernstein HC,Weisenhorn P,Taylor RC,Lee JY,Zucker J,Song HS

    更新日期:2016-11-01 00:00:00

  • Evaluation of BAG3 levels in healthy subjects, hypertensive patients, and hypertensive diabetic patients.

    abstract::BAG3 is a member of human BAG (Bcl-2-associated athanogene) proteins and plays a role in apoptosis, cell adhesion, cytoskeleton remodeling, and autophagy. The aim of this study was to evaluate BAG3 levels in healthy subjects, hypertensive patients, and hypertensive diabetic patients. We enrolled 209 Caucasian adults, ...

    journal_title:Journal of cellular physiology

    pub_type: 临床试验,杂志文章

    doi:10.1002/jcp.26093

    authors: Derosa G,Maffioli P,Rosati A,M M,Basile A,D'Angelo A,Romano D,Sahebkar A,Falco A,Turco MC

    更新日期:2018-03-01 00:00:00

  • TNF-α has both stimulatory and inhibitory effects on mouse monocyte-derived osteoclastogenesis.

    abstract::Phenotypically different osteoclasts may be generated from different subsets of precursors. To what extent the formation of these osteoclasts is influenced or mediated by the inflammatory cytokine TNF-α, is unknown and was investigated in this study. The osteoclast precursors early blasts (CD31hi Ly-6C- ), myeloid bla...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26024

    authors: Cao Y,Jansen IDC,Sprangers S,de Vries TJ,Everts V

    更新日期:2017-12-01 00:00:00

  • New signaling pathways from cancer progression modulators to mRNA expression of matrix metalloproteinases in breast cancer cells.

    abstract::We observed previously that each of seven cancer progression inhibitors suppresses the mRNA expression of some matrix metalloproteinases (MMPs), but stimulates that of others, in breast cancer cells. In the present study we tested the effect of overexpressing other cancer modulators on MMP expression. The MMPs tested ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22694

    authors: Delassus GS,Cho H,Eliceiri GL

    更新日期:2011-12-01 00:00:00

  • Mechanism of zinc uptake by microvilli isolated from human term placenta.

    abstract::The mechanism of zinc (Zn) uptake by microvillous membrane vesicles prepared from human term placenta has been studied. The uptake was complex, with two processes being identified. In the first process, uptake was rapid, reaching equilibrium within 2 min, and was temperature dependent, with a Q10 of 1.5. Equilibrium Z...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041510312

    authors: Aslam N,McArdle HJ

    更新日期:1992-06-01 00:00:00

  • High salt-induced excess reactive oxygen species production resulted in heart tube malformation during gastrulation.

    abstract::An association has been proved between high salt consumption and cardiovascular mortality. In vertebrates, the heart is the first functional organ to be formed. However, it is not clear whether high-salt exposure has an adverse impact on cardiogenesis. Here we report high-salt exposure inhibited basement membrane brea...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26528

    authors: Gao LR,Wang G,Zhang J,Li S,Chuai M,Bao Y,Hocher B,Yang X

    更新日期:2018-09-01 00:00:00

  • Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP-2.

    abstract::The roles of Sonic hedgehog (Shh) and Bone morphogenetic protein-2 (Bmp-2) in osteoblast differentiation were investigated using in vitro cell systems. Recombinant amino-terminal portion of SHH (rSHH-N) dose dependently stimulated ALP activity in C3H10T1/2 and MC3T3-E1 cells. rSHH-N induced expression of Osteocalcin m...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10166

    authors: Yuasa T,Kataoka H,Kinto N,Iwamoto M,Enomoto-Iwamoto M,Iemura S,Ueno N,Shibata Y,Kurosawa H,Yamaguchi A

    更新日期:2002-11-01 00:00:00

  • Expression of transforming growth factor-beta receptor type II and tumorigenicity in human breast adenocarcinoma MCF-7 cells.

    abstract::To analyze transforming growth factor-beta (TGF-beta) response during MCF-7 cell progression, early passage (MCF-7E, < 200 passage) and late passage (MCF-7L, > 500 passage) cells were compared. MCF-7E cells showed an IC50 of approximately 10 ng/ml of TGF-beta1, whereas MCF-7L cells were insensitive. MCF-7E cells conta...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199808)176:2<424::AID-JCP2

    authors: Ko Y,Banerji SS,Liu Y,Li W,Liang J,Soule HD,Pauley RJ,Willson JK,Zborowska E,Brattain MG

    更新日期:1998-08-01 00:00:00

  • Effects of nitric oxide and peroxynitrite on endotoxin-induced leukocyte adhesion to endothelium.

    abstract::Leukocyte accumulation has been shown to be increased in sepsis. Moreover, in inducible nitric oxide synthase (iNOS) knockout mice, a further increase in leukocyte accumulation has been observed during sepsis, suggesting that nitric oxide (NO) may affect leukocyte/endothelial interaction. Accelerated peroxynitrite for...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1128

    authors: Lelamali K,Wang W,Gengaro P,Edelstein C,Schrier RW

    更新日期:2001-09-01 00:00:00

  • The brain cognitive reserve hypothesis: A review with emphasis on the contribution of nuclear medicine neuroimaging techniques.

    abstract::Neuropathological and clinical evidence indicates that the clinical expression of Alzheimer's disease (AD) occurs as neuropathology exceeds the brain reserve capacity. The brain or cognitive reserve (BCR) hypothesis states that high premorbid intelligence, education, and an active and stimulating lifestyle provide res...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28308

    authors: Giovacchini G,Giovannini E,Borsò E,Lazzeri P,Riondato M,Leoncini R,Duce V,Mansi L,Ciarmiello A

    更新日期:2019-02-19 00:00:00

  • Ontogeny of human neutrophil granulocyte alkaline phosphatase.

    abstract::Human embryonic neutrophils (N) in the liver (from 8.5 mm crown-rump length) are alkaline phosphatase (AP) negative during the first trimester of pregnancy. Early bone marrow granulocytes (from eleventh to sixteenth weeks of gestation) behave similarly. Only a small percentage of slightly AP positive cells could be fo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040950313

    authors: Kelemen E,Gulya E,Vass K

    更新日期:1978-06-01 00:00:00