Abstract：
:Diminished microRNA-29b levels have recently been revealed to provoke increased expression and accumulation of extracellular matrix molecules, such as collagens in fibrotic remodeling. Subsequently, the aim of this study was to find out whether microRNA-29b might also regulate human xylosyltransferase (XT)-I expression. XT-I has been characterized previously as a fibrosis biomarker catalyzing the key step of proteoglycan biosynthesis. While we demonstrate that XYLT1 is neither a target of microRNA-29b identified in silico nor a direct 3' untranslated region binding partner of microRNA-29b, transfection of normal human dermal fibroblasts with microRNA-29b inhibitor strongly increased XYLT1 mRNA expression and XT activity. Combined results of the target prediction analysis and additional transfection experiments pointed out that microRNA-29b exerts indirect influence on XT-I by targeting the transcription factor specificity protein 1 (Sp1). We could confirm our hypothesis due to the decrease in XYLT1 promoter activity after Sp1 binding site mutation and the approval of occupancy of these binding sites by Sp1 in vitro. Taken together, a hitherto unidentified pathway of XT-I regulation via microRNA-29b/Sp1 was determined in this study. Our observations will facilitate the understanding of complex molecular fibrotic pathways and provide new opportunities to investigate microRNA-based antifibrotic tools.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Riedel L,Fischer B,Ly TD,Hendig D,Kuhn J,Knabbe C,Faust Idoi
10.1038/s41598-018-36217-2subject
Has Abstractpub_date
2018-12-12 00:00:00pages
17779issue
1issn
2045-2322pii
10.1038/s41598-018-36217-2journal_volume
8pub_type
杂志文章abstract：:Many real networks feature the property of nestedness, i.e. the neighbours of nodes with a few connections are hierarchically nested within the neighbours of nodes with more connections. Despite the abstract simplicity of this notion, various mathematical definitions of nestedness have been proposed, sometimes giving ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76300-1
更新日期：2020-11-16 00:00:00
abstract：:We show a new resonance acceleration scheme for generating ultradense relativistic electron bunches in helical motions and hence emitting brilliant vortical γ-ray pulses in the quantum electrodynamic (QED) regime of circularly-polarized (CP) laser-plasma interactions. Here the combined effects of the radiation reactio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45031
更新日期：2017-03-24 00:00:00
abstract：:Combining chemotherapy and targeted therapies has resulted in an enhanced survival in metastatic colorectal cancer (mCRC) patients. However, the result of this palliative treatment in patients with metachronous peritoneal carcinomatosis (PC) remains unknown. The current population-based study aims to investigate the u...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18632
更新日期：2015-12-21 00:00:00
abstract：:PDCD4, the protein encoded by the tumor suppressor gene PDCD4 (programmed cell death 4) has been implicated in the control of cellular transcription and translation by modulating the activity of specific transcription factors and suppressing the translation of mRNAs with structured 5'-UTRs. Most studies of human PDCD4...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59678-w
更新日期：2020-02-17 00:00:00
abstract：:We propose a new efficient algorithm to simulate magnetic structures numerically. It contains a generative model using a complex-valued neural network to generate k-space information. The output information is hermitized and transformed into real-space spin configurations through an inverse fast Fourier transform. The...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53411-y
更新日期：2019-11-13 00:00:00
abstract：:We show that filamentous fungi can emit green or red light after the accumulation of particulate lanthanide metal-organic frameworks over the cell wall. These new biohybrids present photoluminescence properties that are unaffected by the components of the cell wall. In addition, the fungal cells internalise lanthanide...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43835-x
更新日期：2019-05-13 00:00:00
abstract：:In this paper we present a contactless transient carrier spectroscopy and imaging technique for traps in silicon. At each pixel, we fit the transient decay of the trap emission which allows us to obtain both the trap time constant and trap concentration. Here we show that this technique allows for high-resolution imag...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49804-8
更新日期：2019-10-03 00:00:00
abstract：:Electrochemical capacitors (EC) have received tremendous interest due to their high potential to satisfy the urgent demand in many advanced applications. The development of new electrode materials is considered to be the most promising approach to enhance the EC performance substantially. Herein, we present a high-cap...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06083
更新日期：2014-08-15 00:00:00
abstract：:The hypothalamic-pituitary-adrenal (HPA) axis is an important component of neuroendocrine stress regulation and coping behavior. Transcriptome profiles of the hypothalamus and adrenal gland were assessed to identify molecular pathways and candidate genes for coping behavior in pigs. Ten each of high- (HR) and low- (LR...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49521-2
更新日期：2019-09-10 00:00:00
abstract：:Despite non-structural carbohydrate (NSC) importance for tree productivity and resilience, little is known about their seasonal regulations and trade-off with growth and reproduction. We characterize the seasonal dynamics of NSC in relation to the aboveground phenology and temporal growth patterns of three deciduous M...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57016-3
更新日期：2020-01-15 00:00:00
abstract：:Molecular dynamics (MD) simulations lasting 500 ns were performed in explicit water to investigate the effect of polarization on the binding of ligands to human α-thrombin based on the standard nonpolarizable AMBER force field and the quantum-derived polarized protein-specific charge (PPC). The PPC includes the electr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31488
更新日期：2016-08-10 00:00:00
abstract：:The controversial anti-proliferative effects of Echinacea purpurea (L.) Moench (Asteraceae) might be related to different plant metabolites contained in plant samples, extracts and products. The influence of bacterial endophytes on the synthesis of bioactive compounds in the medicinal plants has been previously demons...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47329-8
更新日期：2019-07-26 00:00:00
abstract：:Dendritic Cells (DCs) recognize infectious non-self molecules and engage the adaptive immune system thereby initiating long lasting, antigen-specific responses. As such, the ability to activate DCs is considered a key tool to enhance the efficacy and quality of vaccination. Here we report a novel immunomodulatory sulf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05969-8
更新日期：2017-07-24 00:00:00
abstract：:Marine macroalgal forests are highly productive and iconic ecosystems, which are seriously threatened by number of factors such as habitat destruction, overgrazing, ocean warming, and pollution. The effect of chronic, but low levels of pollutants on the long-term survival of the canopy-forming algae is not well unders...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73990-5
更新日期：2020-10-20 00:00:00
abstract：:The combined regulation of a network of inhibitory and activating T cell receptors may be a critical step in the development of chronic HCV infection. Ex vivo HCV MHC class I + II tetramer staining and bead-enrichment was performed with baseline and longitudinal PBMC samples of a cohort of patients with acute, chronic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47024-8
更新日期：2019-07-23 00:00:00
abstract：:DNA condensation is a ubiquitous phenomenon in biology, yet the physical basis for it has remained elusive. Here, we have explored the mechanism of DNA condensation through the protamine-DNA interaction, and by examining on it the influence of DNA binding drugs. We observed that the DNA condensation is accompanied by ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41975-8
更新日期：2019-04-10 00:00:00
abstract：:Ultra-fast synchrotron radiation emission can arise from the transverse betatron motion of an electron in a laser plasma wakefield, and the radiation spectral peak is limited to tens of keV. Here, we present a new method for achieving high-energy radiation via accelerated electrons wiggling in an additional laser fiel...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38777-3
更新日期：2019-02-21 00:00:00
abstract：:Ebola virus (EBOV), isolate Makona, was the causative agent of the West African epidemic devastating predominantly Guinea, Liberia and Sierra Leone from 2013-2016. While several experimental vaccine and treatment approaches have been accelerated through human clinical trials, there is still no approved countermeasure ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-19041-y
更新日期：2018-01-16 00:00:00
abstract：:Tissue macrophage-derived apoptosis inhibitor of macrophage (AIM, encoded by cd5l gene) is a circulating protein that has suppressive functions in a broad range of diseases including obesity, liver steatosis, hepatocellular carcinoma (HCC), and acute kidney injury (AKI). In healthy states, high levels of AIM circulate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31580-6
更新日期：2018-09-03 00:00:00
abstract：:We discover and characterise strong quantum scars, or quantum eigenstates resembling classical periodic orbits, in two-dimensional quantum wells perturbed by local impurities. These scars are not explained by ordinary scar theory, which would require the existence of short, moderately unstable periodic orbits in the p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37656
更新日期：2016-11-28 00:00:00
abstract：:The discovery of the insertion of IbT-DNA1 and IbT-DNA2 into the cultivated (hexaploid) sweetpotato [Ipomoea batatas (L.) Lam.] genome constitutes a clear example of an ancient event of Horizontal Gene Transfer (HGT). However, it remains unknown whether the acquisition of both IbT-DNAs by the cultivated sweetpotato oc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48691-3
更新日期：2019-08-29 00:00:00
abstract：:The Kidney Donor Profile Index (KDPI) was introduced in the United States in 2014 to guide the decision making of clinicians with respect to accepting or declining a donated kidney. To evaluate whether the KDPI can be applied to a European cohort, we retrospectively assessed 580 adult patients who underwent renal tran...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47772-7
更新日期：2019-08-02 00:00:00
abstract：:The onset of inversion in the metal-oxide-semiconductor field-effect transistor (MOSFET) takes place when the surface potential is approximately twice the bulk potential. In contrast, the conduction threshold in accumulation mode transistors, such as the oxide thin film transistor (TFT), has remained ambiguous in view...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22567
更新日期：2016-03-02 00:00:00
abstract：:In chronic neurodegenerative syndromes, neurons progressively die through a generalized retraction pattern triggering retrograde axonal degeneration toward the cell bodies, which molecular mechanisms remain elusive. Recent observations suggest that direct activation of pro-apoptotic signaling in axons triggers local d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32777
更新日期：2016-09-08 00:00:00
abstract：:The prognostic value of current multigene assays for breast cancer is limited to hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer. Despite the prognostic significance of immune response-related genes in breast cancer, immune gene signatures have not been incorporated int...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80274-5
更新日期：2021-01-08 00:00:00
abstract：:Western North Pacific tropical cyclone (TC) activity is known to have a significant response to El Niño and Southern Oscillation (ENSO). Among the TC intensity classifications, "super typhoon", is widely used as a symbolic word for warning people about the potential for experiencing the most severe typhoon. This study...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41561-y
更新日期：2019-03-25 00:00:00
abstract：:Single nucleotide polymorphisms (SNPs) and genes associated with susceptibility to hepatitis B virus (HBV) infection that have been identified by genome-wide association studies explain only a limited portion of the known heritability, indicating more genetic variants remain to be discovered. In this study, we adopted...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49777-8
更新日期：2019-09-17 00:00:00
abstract：:In the field of correlative microscopy, light and electron microscopy form a powerful combination for morphological analyses in zoology. Due to sample thickness limitations, these imaging techniques often require sectioning to investigate small animals and thereby suffer from various artefacts. A recently introduced n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60977-5
更新日期：2020-03-03 00:00:00
abstract：:Loss-of-function mutations in TREM2 cause Nasu-Hakola disease (NHD), a rare genetic disease characterized by early-onset dementia with leukoencephalopathy and bone cysts. An NHD-associated mutation, c.482 + 2 T > C, disrupts the splice donor site of intron 3 and causes aberrant skipping of exon 3, resulting in the los...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25204-2
更新日期：2018-05-02 00:00:00
abstract：:While most large river-deltas in the world are facing the risk of subsidence and erosion in the Anthropocene, it is suspected that the Changjiang submerged delta (CSD) could be subjected to the impacts of the world's largest dam, the Three Gorges Dam (TGD). Here we firstly indicate that the CSD went through high accum...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06600
更新日期：2014-10-16 00:00:00
