Abstract:
:The accurate measurement of microscopic force fields is crucial in many branches of science and technology, from biophotonics and mechanobiology to microscopy and optomechanics. These forces are often probed by analysing their influence on the motion of Brownian particles. Here we introduce a powerful algorithm for microscopic force reconstruction via maximum-likelihood-estimator analysis (FORMA) to retrieve the force field acting on a Brownian particle from the analysis of its displacements. FORMA estimates accurately the conservative and non-conservative components of the force field with important advantages over established techniques, being parameter-free, requiring ten-fold less data and executing orders-of-magnitude faster. We demonstrate FORMA performance using optical tweezers, showing how, outperforming other available techniques, it can identify and characterise stable and unstable equilibrium points in generic force fields. Thanks to its high performance, FORMA can accelerate the development of microscopic and nanoscopic force transducers for physics, biology and engineering.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Pérez García L,Donlucas Pérez J,Volpe G,V Arzola A,Volpe Gdoi
10.1038/s41467-018-07437-xsubject
Has Abstractpub_date
2018-12-04 00:00:00pages
5166issue
1issn
2041-1723pii
10.1038/s41467-018-07437-xjournal_volume
9pub_type
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