Inhibition of lactate dehydrogenase A suppresses inflammatory response in RAW 264.7 macrophages.

Abstract:

:Lactate is an important metabolite in cellular metabolism and fluctuates in certain disease conditions including cancer and immune diseases. It was hypothesized that a decrease in lactate would modulate the inflammatory response elicited by lipopolysaccharides (LPS) in macrophages. When RAW 264.7 macrophages were treated with FX11, a specific lactate dehydrogenase (LDHA) inhibitor, the expression of the cytokines, inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX‑2) was downregulated due to reduced cellular lactate levels. Genetic suppression of LDHA by small interfering RNA (siRNA) downregulated the LPS‑activated expression of interleukin (IL)‑6, iNOS, and COX‑2, and reduced the production of IL‑6 and nitrites. Pharmacological and genetic suppression of LDHA inhibited the phosphorylation of p38 mitogen‑activated protein kinase. Microarray gene expression profile demonstrated that the genes involved in cell proliferation and inflammation were mainly altered by siRNA‑mediated LDHA suppression. Collectively, the present observations suggest that lactate may be an important metabolite and implicated in regulation of inflammatory response.

journal_name

Mol Med Rep

authors

Song YJ,Kim A,Kim GT,Yu HY,Lee ES,Park MJ,Kim YJ,Shim SM,Park TS

doi

10.3892/mmr.2018.9678

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

629-637

issue

1

eissn

1791-2997

issn

1791-3004

journal_volume

19

pub_type

杂志文章
  • Purple sweet potato color attenuates high fat-induced neuroinflammation in mouse brain by inhibiting MAPK and NF-κB activation.

    abstract::Purple sweet potato color (PSPC) is a natural anthocyanin pigment that is derived from purple sweet potato storage roots. PSPC possesses a variety of biological activities, including antioxidant, anti‑inflammatory and neuroprotective effects; however, the detailed effects of PSPC on high‑fat diet (HFD)‑induced neuroin...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8440

    authors: Li J,Shi Z,Mi Y

    更新日期:2018-03-01 00:00:00

  • Astragaloside Ⅳ reduces the expression level of P-glycoprotein in multidrug-resistant human hepatic cancer cell lines.

    abstract::Astragaloside is a saponin widely used in traditional Chinese medicine and has been reported to be a potent multidrug resistance (MDR) reversal agent. The present study investigated the role of astragaloside Ⅳ (ASIV) in the regulation of P-glycoprotein (P-gp, encoded by the mdr1 gene) and its effect on the reversal of...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2074

    authors: Wang PP,Xu DJ,Huang C,Wang WP,Xu WK

    更新日期:2014-06-01 00:00:00

  • Identification of key candidate genes involved in melanoma metastasis.

    abstract::Metastasis is the most lethal stage of cancer progression. The present study aimed to investigate the underlying molecular mechanisms of melanoma metastasis using bioinformatics. Using the microarray dataset GSE8401 from the Gene Expression Omnibus database, which included 52 biopsy specimens from patients with melano...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10314

    authors: Chen J,Wu F,Shi Y,Yang D,Xu M,Lai Y,Liu Y

    更新日期:2019-08-01 00:00:00

  • Effects of isomaltulose on insulin resistance and metabolites in patients with non‑alcoholic fatty liver disease: A metabolomic analysis.

    abstract::Insulin resistance is associated with a poor prognosis in non‑alcoholic fatty liver disease (NAFLD) patients. Isomaltulose, a naturally‑occurring disaccharide, is reported to improve glucose and lipid metabolisms in obese patients. The present study aimed to investigate the effects of isomaltulose on insulin resistanc...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,随机对照试验

    doi:10.3892/mmr.2018.9223

    authors: Kawaguchi T,Nakano D,Oriishi T,Torimura T

    更新日期:2018-08-01 00:00:00

  • Silencing long non‑coding RNA NEAT1 suppresses the tumorigenesis of infantile hemangioma by competitively binding miR‑33a‑5p to stimulate HIF1α/NF‑κB pathway.

    abstract::Infantile hemangioma (IH) is one of the most common vascular tumors that occurs during childhood, but its pathogenesis is currently not completely understood. Even though lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) plays vital roles in tumorigenesis of malignant tumors, its roles in IH remain unclear. The...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11409

    authors: Yu L,Shu H,Xing L,Lv MX,Li L,Xie YC,Zhang Z,Zhang L,Xie YY

    更新日期:2020-10-01 00:00:00

  • N‑terminal truncated peroxisome proliferator‑activated receptor‑γ coactivator‑1α alleviates phenylephrine‑induced mitochondrial dysfunction and decreases lipid droplet accumulation in neonatal rat cardiomyocytes.

    abstract::N‑terminal truncated peroxisome proliferator‑activated receptor‑γ coactivator‑1α (NT‑PGC‑1α) is an alternative splice variant of PGC‑1α. NT‑PGC‑1α exhibits stronger anti‑obesity effects in adipose tissue than PGC‑1α; however, NT‑PGC‑1α has not yet been investigated in neonatal rat cardiomyocytes (NRCMs). The present s...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9158

    authors: Liu Z,Hua J,Cai W,Zhan Q,Lai W,Zeng Q,Ren H,Xu D

    更新日期:2018-08-01 00:00:00

  • Arginase inhibition protects against hypoxia‑induced pulmonary arterial hypertension.

    abstract::The present study aimed to determine the role of arginase (Arg) in pulmonary arterial hypertension (PAH). In vitro, human pulmonary artery smooth muscle cells (HPASMCs) were cultured under hypoxic conditions with, or without, the Arg inhibitor, S‑(2‑boronoethyl)‑l‑cysteine (BEC), for 48 h, following which the prolifer...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3994

    authors: Jiang W,Sun B,Song X,Zheng Y,Wang L,Wang T,Liu S

    更新日期:2015-09-01 00:00:00

  • Mass spectrometry-based proteomic analysis of Kashin-Beck disease.

    abstract::Kashin-Beck disease (KBD) is a degenerative osteoarticular disease of unknown etiology. The management of KBD would benefit from the identification of the biomarkers related to this disease. In this study, mass spectrometry (MS)-based proteomic profiling was used to identify potential biomarkers of the disease. One hu...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2010.327

    authors: Du J,Wu X,Zhang H,Wang S,Tan W,Guo X

    更新日期:2010-09-01 00:00:00

  • Lentivirally overexpressed T-bet regulates T-helper cell lineage commitment in chronic hepatitis B patients.

    abstract::Chronic hepatitis B virus (HBV) infection is commonly considered to occur as a result of disturbance of the immune system. T-box expressed in T cells (T-bet) is an essential transcription factor for T helper (Th) cell differentiation and function. The aim of this study was to investigate the effect of T-bet overexpres...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.905

    authors: Liu X,Tang Z,Zhang Y,Hu J,Li D,Zang G,Yu Y

    更新日期:2012-08-01 00:00:00

  • Genistein attenuates renal fibrosis in streptozotocin‑induced diabetic rats.

    abstract::The present study aimed to investigate the antifibrogenic effects of genistein (GEN) on the kidney in streptozotocin (STZ)‑induced diabetic rats and to determine the associated mechanisms. Rats were randomized into four groups: Normal control (N), STZ (S), L (STZ + low‑dose GEN) and H (STZ + high‑dose GEN). After 8 we...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9635

    authors: Jia Q,Yang R,Liu XF,Ma SF,Wang L

    更新日期:2019-01-01 00:00:00

  • Effects of acupuncture on the gene expression profile of lung tissue from normal rats.

    abstract::Acupuncture has been demonstrated to be an effective treatment for various diseases. However, little attention has been paid to its physiological influences, especially on the changes in protein and mRNA levels following acupuncture treatment under normal conditions. In this study, we investigated the gene expression ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.909

    authors: Yin LM,Wang Y,Wang Y,Xu YD,Liu YY,Jin WR,Zhang QH,Yang YQ

    更新日期:2012-08-01 00:00:00

  • Proliferation of human hepatocellular carcinoma cells from surgically resected specimens under conditionally reprogrammed culture.

    abstract::Hepatocellular carcinoma (HCC) is the third most common cause of cancer mortality worldwide, which is partially due to the lack of appropriate therapeutic options. The development of HCC is accompanied with unique and continuous genomic and epigenetic modifications. Therefore, the absence of a personalized and reprodu...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10160

    authors: Wang Z,Bi B,Song H,Liu L,Zheng H,Wang S,Shen Z

    更新日期:2019-06-01 00:00:00

  • Sulforaphane prevents bleomycin‑induced pulmonary fibrosis in mice by inhibiting oxidative stress via nuclear factor erythroid 2‑related factor‑2 activation.

    abstract::Lung fibrosis is associated with inflammation, apoptosis and oxidative damage. The transcription factor nuclear factor erythroid 2‑related factor‑2 (Nrf2) prevents damage to cells from oxidative stress by regulating the expression of antioxidant proteins. Sulforaphane (SFN), an Nrf2 activator, additionally regulates e...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6546

    authors: Yan B,Ma Z,Shi S,Hu Y,Ma T,Rong G,Yang J

    更新日期:2017-06-01 00:00:00

  • Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues.

    abstract::Activation of renin-angiotensin system (RAS) is one of the pathological mechanisms associated with myocardial ischemia-reperfusion injury following resuscitation. The present study aimed to determine whether erythropoietin (EPO) improves post‑resuscitation myocardial dysfunction and how it affects the renin‑angiotensi...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8473

    authors: Zhou H,Huang J,Zhu L,Cao Y

    更新日期:2018-04-01 00:00:00

  • Puerarin promotes the viability and differentiation of MC3T3‑E1 cells by miR‑204‑regulated Runx2 upregulation.

    abstract::Puerarin has attracted increasing attention because of its beneficial effects on anti‑osteoporosis, but the molecular mechanisms underlying its actions on osteoblasts are not fully understood. The current study aimed to investigate the effect of puerarin on the cell viability and differentiation of mouse MC3T3‑E1 oste...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7386

    authors: Zhan XQ,Zeng XW,Zhang YY,Feng Q,Zhao FM,Jiang ZQ,Sun C

    更新日期:2017-11-01 00:00:00

  • MicroRNA‑296 targets AKT2 in pancreatic cancer and functions as a potential tumor suppressor.

    abstract::Although microRNA-296 (miR-296) has been studied in various types of human cancer, its expression, biological role and mechanism of action in pancreatic cancer remains to be elucidated. The aim of the current study was to investigate the expression level, possible roles and underlying molecular mechanisms of miR‑296 i...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6602

    authors: Li H,Li J,Shi B,Chen F

    更新日期:2017-07-01 00:00:00

  • Effects of matrine on oval cell‑mediated liver regeneration and expression of RBP‑Jκ and HES1.

    abstract::In the present study a rat model of oval cell‑mediated liver regeneration was constructed to examine the molecular mechanisms of matrine in oval cell‑mediated liver regeneration and the effects of matrine on hepatic function and the expression of OV6 protein and recombination signal sequence‑binding protein Jκ (RBP‑Jκ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1398

    authors: Yang ZY,Wang L,Hou YX,Wang XB

    更新日期:2013-05-01 00:00:00

  • Anti-tumor effect of combining CC chemokine 22 and an anti-CD25 antibody on myeloma cells implanted subcutaneously into mice.

    abstract::Chemokines are known to have anti-tumor effects due to their chemoattractant properties, which stimulate the accumulation of infiltrating immune cells in tumors. CCL22 (macrophage-derived chemokine, MDC) attracts killer T?cells, helper T cells and antigen-presenting cells expressing the CCL22 receptor, CCR4. Thus, CCL...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr_00000171

    authors: Cho S,Koizumi K,Takeno N,Kato S,Yamada M,Hashimoto I,Sakurai H,Tsukada K,Saiki I

    更新日期:2009-09-01 00:00:00

  • miR‑222 regulates brain injury and inflammation following intracerebral hemorrhage by targeting ITGB8.

    abstract::Intracerebral hemorrhage (ICH) is a disease associated with high mortality and morbidity. MicroRNAs (miRNAs) have been reported to be associated with the pathogenesis of numerous cerebrovascular diseases, including ICH. miR‑222 has been revealed to play important roles in various physiological and pathological process...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10903

    authors: Bai YY,Niu JZ

    更新日期:2020-03-01 00:00:00

  • Autophagy is essential for flavopiridol‑induced cytotoxicity against MCF‑7 breast cancer cells.

    abstract::Flavopiridol (FP) exerts antitumoral effects by triggering tumor cell cycle arrest and cytotoxicity in human breast cancer cell lines. The potent antitumor activity of FP is through its inhibition of cyclin‑dependent kinases; however, this may not be the only mechanism of action. The present study aimed to investigate...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7815

    authors: Wang S,Wang K,Wang H,Han J,Sun H

    更新日期:2017-12-01 00:00:00

  • Function of Krüppel‑like factor 2 in the shear stress‑induced cell differentiation of endothelial progenitor cells to endothelial cells.

    abstract::The present study aimed to evaluate the effects of Krüppel‑like factor 2 (KLF2) on the differentiation of endothelial progenitor cells (EPCs) to endothelial cells (ECs) induced by shear stress, and to investigate the corresponding mechanisms. Cultured rat late EPCs were exposed to shear stress (12 dyn/cm2) for differe...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9819

    authors: Chu HR,Sun YC,Gao Y,Guan XM,Yan H,Cui XD,Zhang XY,Li X,Li H,Cheng M

    更新日期:2019-03-01 00:00:00

  • A germline mutation in the miR‑125a coding region reduces miR‑125a expression and is associated with human gastric cancer.

    abstract::MicroRNAs (miRNAs) are small non-coding RNAs that inhibit the expression of target protein-coding genes, most often at the post-transcriptional level. miRNAs are often found to be misregulated in human cancer and they can act as potent oncogenes or tumor suppressor genes. In this study, we found that a germline mutati...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2441

    authors: Shang H,Wang T,Shang F,Huang KM,Li YQ

    更新日期:2014-10-01 00:00:00

  • Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling.

    abstract::A limited number of studies have revealed that adding kidneys to liver perfusion may maintain an improved physiological balance; however, the underlying mechanism remains to be elucidated. The preset study confirmed the protective role of this new model and investigated the underlying mechanisms. Methods: A total of 1...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10379

    authors: Li J,Jia J,He N,Jiang L,Yu H,Li H,Peng Y,Xie H,Zhou L,Zheng S

    更新日期:2019-08-01 00:00:00

  • Effects of reduced β2 glycoprotein I on high glucose‑induced cell death in HUVECs.

    abstract::Reduced β2 glycoprotein I (β2GPI) has been demonstrated to exhibit a beneficial effect in diabetic atherosclerosis and retinal neovascularization. However, the effect of reduced β2GPI on vascular disorders in diabetic mellitus (DM) remains to be elucidated. The present study established a high glucose‑induced injury m...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7065

    authors: Zhang JY,Ma J,Yu P,Tang GJ,Li CJ,Yu DM,Zhang QM

    更新日期:2017-10-01 00:00:00

  • microRNA‑202 suppresses MYCN expression under the control of E2F1 in the neuroblastoma cell line LAN‑5.

    abstract::microRNAs (miRNAs) are small non‑coding RNAs that regulate gene expression by binding to the untranslated regions (UTRs) of target mRNAs. Bioinformatic software predicted that MYCN, a gene overexpressed in aggressive neuroblastoma cells, is a target gene of miRNA‑202 (miR‑202) and that the promoter region of miR‑202 c...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1845

    authors: Li YG,He JH,Yu L,Hang ZP,Li W,Shun WH,Huang GX

    更新日期:2014-02-01 00:00:00

  • Antioxidant effect of angiotensin (1‑7) in the protection of pancreatic β cell function.

    abstract::It is well known that the local renin-angiotensin system (RAS) is activated in the diabetic state, which results in an increase in the level of oxidative stress injury to pancreatic β cells. The angiotensin‑converting enzyme 2 (ACE2)/angiotensin (1‑7) [Ang (1‑7)]/Mas axis is a negative regulator of the classical renin...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5514

    authors: Zhang F,Liu C,Wang L,Cao X,Wang YY,Yang JK

    更新日期:2016-09-01 00:00:00

  • Effect of rutin on spinal cord injury through inhibition of the expression of MIP-2 and activation of MMP-9, and downregulation of Akt phosphorylation.

    abstract::Rutin has extensive pharmacological activities, including antibacterial and anti-inflammatory activities, cooling of the blood to inhibit bleeding, reducing capillary wall fragility and anti-influenza activities. However, whether rutin can ameliorate neuropathic function in spinal cord injury (SCI) in constriction-ind...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4357

    authors: Zhang P,Ma X

    更新日期:2015-11-01 00:00:00

  • Gli affects the stemness and prognosis of epithelial ovarian cancer via homeobox protein NANOG.

    abstract::Gli proteins are key transcription factors of the Hedgehog (HH) signaling pathway, which is associated with tumorigenesis and drug resistance. However, the role of the HH signaling pathway in epithelial ovarian cancer (EOC) remains unclear. Studies have demonstrated that in some tumors, homeobox protein NANOG (NANOG),...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11767

    authors: Zhao H,Li N,Pang Y,Zhao J,Wu X

    更新日期:2021-02-01 00:00:00

  • Low expression of phosphatase and tensin homolog in clear‑cell renal cell carcinoma contributes to chemoresistance through activating the Akt/HDM2 signaling pathway.

    abstract::Clear-cell renal cell carcinoma (CCRCC) is the most frequent primary malignancy in the adult kidney. Most patients with advanced CCRCC have poor prognosis as CCRCC remains resistant to chemotherapy. The present study explored the possible mechanism underlying CCRCC resistance to chemotherapy and found that loss of PTE...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3740

    authors: Chen J,Zhu H,Zhang Y,Cui MH,Han LY,Jia ZH,Wang L,Teng H,Miao LN

    更新日期:2015-08-01 00:00:00

  • Comprehensive bioinformatics analysis of critical lncRNAs, mRNAs and miRNAs in non‑alcoholic fatty liver disease.

    abstract::Non‑alcoholic fatty liver disease (NAFLD) is the most common fatty liver disease in developed countries, in which fat accumulation in the liver is induced by non‑alcoholic factors. The present study was conducted to identify NAFLD‑associated long non‑coding RNAs (lncRNAs), mRNAs and microRNAs (miRNAs). The microarray ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9931

    authors: Wu H,Song X,Ling Y,Zhou J,Tao Z,Shen Y

    更新日期:2019-04-01 00:00:00