Abstract:
:Silver (Ag+) and copper (Cu+) ions have been used for centuries in industry, as well as antimicrobial agents in agriculture and health care. Nowadays, Ag+ is also widely used in the field of nanotechnology. Yet, the underlying mechanisms driving toxicity of Ag+ ions in vivo are poorly characterized. It is well known that exposure to excess metal impairs the photosynthetic apparatus of plants and algae. Here, we show that the light-harvesting complex II (LH2) is the primary target of Ag+ and Cu+ exposure in the purple bacterium Rubrivivax gelatinosus Ag+ and Cu+ specifically inactivate the 800-nm absorbing bacteriochlorophyll a (B800), while Ni2+ or Cd2+ treatment had no effect. This was further supported by analyses of CuSO4- or AgNO3-treated membrane proteins. Indeed, this treatment induced changes in the LH2 absorption spectrum related to the disruption of the interaction of B800 molecules with the LH2 protein. This caused the release of B800 molecules and subsequently impacted the spectral properties of the carotenoids within the 850-nm absorbing LH2. Moreover, previous studies have suggested that Ag+ can affect the respiratory chain in mitochondria and bacteria. Our data demonstrated that exposure to Ag+, both in vivo and in vitro, caused a decrease of cytochrome c oxidase and succinate dehydrogenase activities. Ag+ inhibition of these respiratory complexes was also observed in Escherichia coli, but not in Bacillus subtilisIMPORTANCE The use of metal ions represents a serious threat to the environment and to all living organisms because of the acute toxicity of these ions. Nowadays, silver nanoparticles are one of the most widely used nanoparticles in various industrial and health applications. The antimicrobial effect of nanoparticles is in part related to the released Ag+ ions and their ability to interact with bacterial membranes. Here, we identify, both in vitro and in vivo, specific targets of Ag+ ions within the membrane of bacteria. This include complexes involved in photosynthesis, but also complexes involved in respiration.
journal_name
mBiojournal_title
mBioauthors
Tambosi R,Liotenberg S,Bourbon ML,Steunou AS,Babot M,Durand A,Kebaili N,Ouchane Sdoi
10.1128/mBio.01535-18subject
Has Abstractpub_date
2018-11-20 00:00:00issue
6issn
2150-7511pii
mBio.01535-18journal_volume
9pub_type
杂志文章相关文献
mBio文献大全abstract::This research analyzed six Aspergillus fumigatus genes encoding putative efflux proteins for their roles as transporters. TheA. fumigatus genes abcA, abcC, abcF, abcG, abcH, and abcI were cloned into plasmids and overexpressed in a Saccharomyces cerevisiae strain in which the highly active endogenous ABC transporter g...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00338-20
更新日期:2020-03-24 00:00:00
abstract::A central question in mechanobiology is how cellular-scale structures are established and regulated. In bacteria, the cell envelope is essential for mechanical integrity, protecting against environmental stresses and bearing the load from high turgor pressures. Trivedi et al. (mBio 9:e01340-18, 2018, https://doi.org/1...
journal_title:mBio
pub_type: 评论,杂志文章
doi:10.1128/mBio.02127-18
更新日期:2018-10-23 00:00:00
abstract::5-Aminosalicylic acid (5-ASA), a peroxisome proliferator-activated receptor gamma (PPAR-γ) agonist, is a widely used first-line medication for the treatment of ulcerative colitis, but its anti-inflammatory mechanism is not fully resolved. Here, we show that 5-ASA ameliorates colitis in dextran sulfate sodium (DSS)-tre...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.03227-20
更新日期:2021-01-19 00:00:00
abstract:UNLABELLED:The human pathogen Streptococcus pneumoniae (pneumococcus) exhibits a high degree of genomic diversity and plasticity. Isolates with high genomic similarity are grouped into lineages that undergo homologous recombination at variable rates. PMEN1 is a pandemic, multidrug-resistant lineage. Heterologous gene e...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00173-15
更新日期:2015-06-16 00:00:00
abstract:UNLABELLED:No studies have examined the relationships between bacterial communities along sites of the upper aerodigestive tract of an individual subject. Our objective was to perform an intrasubject and intersite analysis to determine the contributions of two upper mucosal sites (mouth and nose) as source communities ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00037-15
更新日期:2015-03-03 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) is the causative agent of human Kaposi's sarcoma, a tumor that arises from endothelial cells, as well as two B cell lymphoproliferative diseases, primary effusion lymphoma and multicentric Castleman's disease. KSHV utilizes a variety of mechanisms to evade host ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01499-15
更新日期:2015-12-08 00:00:00
abstract::The opportunistic fungal pathogen Cryptococcus neoformans must adapt to the mammalian environment to establish an infection. Proteins facilitating adaptation to novel environments, such as chaperones, may be required for virulence. In this study, we identified a novel mitochondrial co-chaperone, Mrj1 (mitochondrial re...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01127-20
更新日期:2020-06-09 00:00:00
abstract::The pathogenic species of Cryptococcus are a major cause of mortality owing to severe infections in immunocompromised as well as immunocompetent individuals. Although antifungal treatment is usually effective, many patients relapse after treatment, and in such cases, comparative analyses of the genomes of incident and...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00166-17
更新日期:2017-03-07 00:00:00
abstract::Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes blinding trachoma and sexually transmitted disease afflicting hundreds of millions of people globally. A fundamental but poorly understood pathophysiological characteristic of chlamydial infection is the propensity to cause persistent in...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01902-20
更新日期:2020-08-18 00:00:00
abstract::Candida albicans has developmental programs that govern transitions between yeast and filamentous morphologies and between unattached and biofilm lifestyles. Here, we report that filamentation, intercellular adherence, and biofilm development were inhibited during interactions between Candida albicans and Pseudomonas ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00526-12
更新日期:2013-01-29 00:00:00
abstract::Malaria parasites increase their host erythrocyte's permeability to various nutrients, fueling intracellular pathogen development and replication. The plasmodial surface anion channel (PSAC) mediates this uptake and is linked to the parasite-encoded RhopH complex, consisting of CLAG3, RhopH2, and RhopH3. While interac...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01354-20
更新日期:2020-09-08 00:00:00
abstract::In bacteria and eukaryotes alike, proper cellular physiology relies on robust subcellular organization. For the phototrophic purple nonsulfur bacteria (PNSB), this organization entails the use of a light-harvesting, membrane-bound compartment known as the intracytoplasmic membrane (ICM). Here we show that ICMs are spa...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00780-18
更新日期:2018-07-03 00:00:00
abstract::Transposon insertion sequencing (TIS) is a powerful high-throughput genetic technique that is transforming functional genomics in prokaryotes, because it enables genome-wide mapping of the determinants of fitness. However, current approaches for analyzing TIS data assume that selective pressures are constant over time...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01581-17
更新日期:2017-10-03 00:00:00
abstract:UNLABELLED:Glycoside hydrolases (GHs) are key enzymes in the depolymerization of plant-derived cellulose, a process central to the global carbon cycle and the conversion of plant biomass to fuels and chemicals. A limited number of GH families hydrolyze crystalline cellulose, often by a processive mechanism along the ce...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01106-16
更新日期:2016-08-23 00:00:00
abstract::Symbiotic microorganisms can have a profound impact on the host physiology and behavior, and novel relationships between symbionts and their hosts are continually discovered. A colony of social ants consists of various castes that exhibit distinct lifestyles and is, thus, a unique model for investigating how symbionts...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00408-20
更新日期:2020-04-21 00:00:00
abstract::The alphaproteobacterial general stress response is governed by a conserved partner-switching mechanism that is triggered by phosphorylation of the response regulator PhyR. In the model organism Caulobacter crescentus, PhyR was proposed to be phosphorylated by the histidine kinase PhyK, but biochemical evidence in sup...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00809-18
更新日期:2018-05-22 00:00:00
abstract:UNLABELLED:Susceptibility to mouse adenovirus type 1 (MAV-1) is mouse strain dependent; susceptible mice die from hemorrhagic encephalomyelitis. The MAV-1 susceptibility quantitative trait locus Msq1 accounts for ~40% of the phenotypic (brain viral load) variance that occurs between resistant BALB/c and susceptible SJL...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00131-12
更新日期:2012-05-29 00:00:00
abstract::Antimicrobial resistance is a major health threat as it limits treatment options for infection. At the forefront of this serious issue is Acinetobacter baumannii, a Gram-negative opportunistic pathogen that exhibits the remarkable ability to resist antibiotics through multiple mechanisms. As bacterial ribosomes repres...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.03117-19
更新日期:2020-01-21 00:00:00
abstract::Sodium-translocating NADH:quinone oxidoreductase (Na+-NQR) functions as a unique redox-driven sodium pump, generating membrane potential, which is related to aminoglycoside antibiotic resistance. However, whether it modulates other metabolisms to confer antibiotic resistance is unknown. The present study showed that l...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02086-20
更新日期:2020-11-17 00:00:00
abstract::Escherichia coli sequence type 131 (ST131), a pandemic clone responsible for the high incidence of extraintestinal pathogenic E. coli (ExPEC) infections, has been known widely for its contribution to the worldwide dissemination of multidrug resistance. Although other ExPEC-associated and extended-spectrum-β-lactamase ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01596-17
更新日期:2017-10-24 00:00:00
abstract::Maternal infection during pregnancy with a wide range of RNA and DNA viruses is associated with increased risk for schizophrenia and autism in their offspring. A common feature in these exposures is that virus replication induces innate immunity through interaction with Toll-like receptors (TLRs). We employed a mouse ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00176-10
更新日期:2010-10-05 00:00:00
abstract::The type III secretion system (T3SS) forms the functional core of injectisomes, protein transporters that allow bacteria to deliver virulence factors into their hosts for infection, and flagella, which are critical for many pathogens to reach the site of infection. In spite of intensive genetic and biochemical studies...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00988-18
更新日期:2018-06-26 00:00:00
abstract::Enterohemorrhagic Escherichia coli (EHEC) is a major cause of foodborne gastrointestinal illness. EHEC uses a specialized type III secretion system (T3SS) to form attaching and effacing lesions in the colonic epithelium and outcompete commensal gut microbiota to cause disease. A recent report in mBio (E. A. Cameron, M...
journal_title:mBio
pub_type: 评论,杂志文章
doi:10.1128/mBio.00115-19
更新日期:2019-02-26 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen and the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. P. aeruginosa infections are difficult to treat due to a number of antibiotic resistance mechanisms and the organism's propensity to form multicellular biofilms. Epidemic strains of P....
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01828-18
更新日期:2019-01-29 00:00:00
abstract::Type II polyketides are a group of secondary metabolites with various biological activities. In nature, biosynthesis of type II polyketides involves multiple enzymatic steps whereby key enzymes, including ketoacyl-synthase (KSα), chain length factor (KSβ), and acyl carrier protein (ACP), are utilized to elongate the p...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01334-20
更新日期:2020-09-15 00:00:00
abstract::The four dengue virus serotypes (DENV1 to 4) cause dengue, a major public health problem worldwide. Individuals exposed to primary DENV infections develop serotype-specific neutralizing antibodies, including strongly neutralizing antibodies targeting quaternary epitopes. To date, no studies have measured the levels an...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.01205-17
更新日期:2017-09-19 00:00:00
abstract:UNLABELLED:The ability to cultivate food is an innovation that has produced some of the most successful ecological strategies on the planet. Although most well recognized in humans, where agriculture represents a defining feature of civilization, species of ants, beetles, and termites have also independently evolved sy...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02077-14
更新日期:2014-11-18 00:00:00
abstract::The human antibody repertoire has an exceptionally large capacity to recognize new or changing antigens through combinatorial and junctional diversity established at the time of V(D)J recombination and through somatic hypermutation. Influenza viruses exhibit a relentless capacity to escape the human antibody response ...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00213-12
更新日期:2012-07-31 00:00:00
abstract::Membrane repair emerges as an innate defense protecting target cells against bacterial pore-forming toxins. Here, we report the first paradigm of Ca2+-dependent repair following attack by a small β-pore-forming toxin, namely, plasmid-encoded phobalysin of Photobacterium damselae subsp. damselae In striking contrast, V...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.02083-16
更新日期:2017-02-14 00:00:00
abstract:UNLABELLED:Toll-like receptor 4 is thought to have a primary role in host defense against Escherichia coli bladder colonization, based on mouse models of urinary tract infection using C3H/HeJ female mice. This strain carries a point mutation in the Tlr4 gene, which renders the mice unresponsive to lipopolysaccharide (L...
journal_title:mBio
pub_type: 杂志文章
doi:10.1128/mBio.00094-11
更新日期:2011-05-31 00:00:00