Abstract:
:Sterol 14α-demethylases (CYP51) are cytochrome P450 enzymes essential for sterol biosynthesis in eukaryotes and therapeutic targets for antifungal azoles. Multiple attempts to repurpose antifungals for treatment of human infections with protozoa (Trypanosomatidae) have been undertaken, yet so far none of them have revealed sufficient efficacy. VNI and its derivative VFV are two potent experimental inhibitors of Trypanosomatidae CYP51, effective in vivo against Chagas disease, visceral leishmaniasis, and sleeping sickness and currently under consideration as antiprotozoal drug candidates. However, VNI is less potent against Leishmania and drug-resistant strains of Trypanosoma cruzi and VFV, while displaying a broader spectrum of antiprotozoal activity, and is metabolically less stable. In this work we have designed, synthesized, and characterized a set of close analogues and identified two new compounds (7 and 9) that exceed VNI/VFV in their spectra of antiprotozoal activity, microsomal stability, and pharmacokinetics (tissue distribution in particular) and, like VNI/VFV, reveal no acute toxicity.
journal_name
J Med Chemjournal_title
Journal of medicinal chemistryauthors
Friggeri L,Hargrove TY,Rachakonda G,Blobaum AL,Fisher P,de Oliveira GM,da Silva CF,Soeiro MNC,Nes WD,Lindsley CW,Villalta F,Guengerich FP,Lepesheva GIdoi
10.1021/acs.jmedchem.8b01671subject
Has Abstractpub_date
2018-12-13 00:00:00pages
10910-10921issue
23eissn
0022-2623issn
1520-4804journal_volume
61pub_type
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