Abstract:
:Elg1, the major subunit of a Replication Factor C-like complex, is critical to ensure genomic stability during DNA replication, and is implicated in controlling chromatin structure. We investigated the consequences of Elg1 loss for the dynamics of chromatin re-formation following DNA replication. Measurement of Okazaki fragment length and the micrococcal nuclease sensitivity of newly replicated DNA revealed a defect in nucleosome organization in the absence of Elg1. Using a proteomic approach to identify Elg1 binding partners, we discovered that Elg1 interacts with Rtt106, a histone chaperone implicated in replication-coupled nucleosome assembly that also regulates transcription. A central role for Elg1 is the unloading of PCNA from chromatin following DNA replication, so we examined the relative importance of Rtt106 and PCNA unloading for chromatin reassembly following DNA replication. We find that the major cause of the chromatin organization defects of an ELG1 mutant is PCNA retention on DNA following replication, with Rtt106-Elg1 interaction potentially playing a contributory role.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Gali VK,Dickerson D,Katou Y,Fujiki K,Shirahige K,Owen-Hughes T,Kubota T,Donaldson ADdoi
10.1371/journal.pgen.1007783subject
Has Abstractpub_date
2018-11-12 00:00:00pages
e1007783issue
11eissn
1553-7390issn
1553-7404pii
PGENETICS-D-18-01236journal_volume
14pub_type
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