Abstract：
:Recent advances in the field of Tissue Engineering allowed to control the three-dimensional organization of engineered constructs. Cell pattern imaging and in vivo follow-up remain a major hurdle in in situ bioprinting onto deep tissues. Magnetic Resonance Imaging (MRI) associated with Micron-sized superParamagnetic Iron Oxide (MPIO) particles constitutes a non-invasive method for tracking cells in vivo. To date, no studies have utilized Cellular MRI as a tool to follow cell patterns obtained via bioprinting technologies. Laser-Assisted Bioprinting (LAB) has been increasingly recognized as a new and exciting addition to the bioprinting's arsenal, due to its rapidity, precision and ability to print viable cells. This non-contact technology has been successfully used in recent in vivo applications. The aim of this study was to assess the methodology of tracking MPIO-labeled stem cells using MRI after organizing them by Laser-Assisted Bioprinting. Optimal MPIO concentrations for tracking bioprinted cells were determined. Accuracy of printed patterns was compared using MRI and confocal microscopy. Cell densities within the patterns and MRI signals were correlated. MRI enabled to detect cell patterns after in situ bioprinting onto a mouse calvarial defect. Results demonstrate that MRI combined with MPIO cell labeling is a valuable technique to track bioprinted cells in vitro and in animal models.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kérourédan O,Ribot EJ,Fricain JC,Devillard R,Miraux Sdoi
10.1038/s41598-018-34226-9subject
Has Abstractpub_date
2018-10-25 00:00:00pages
15777issue
1issn
2045-2322pii
10.1038/s41598-018-34226-9journal_volume
8pub_type
杂志文章abstract：:Whereas epidemiologic studies consistently identify different rates and types of problematic behavior in boys and girls, there has been little research examining the ecocultural context in which these gender differences in child behavior problems develop, especially in non-Western settings. This qualitative study in r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43972-3
更新日期：2019-05-21 00:00:00
abstract：:Although the clinical importance of extragraft bone formation (ExGBF) and bridging (ExGBB) has been reported, few studies have investigated the biomechanical influences of ExGBF on the motion segment. In this study, ExGBF was simulated at the C5-C6 motion segment after anterior cervical discectomy and fusion using a d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54785-9
更新日期：2019-12-11 00:00:00
abstract：:Urinary incontinence of idiopathic nature is a common complication of bladder cancer, yet, the mechanisms underlying changes in bladder contractility associated with cancer are not known. Here by using tensiometry on detrusor smooth muscle (DSM) strips from normal rats and rats with bladder cancer induced by known uro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76653-7
更新日期：2020-11-12 00:00:00
abstract：:Lymph node (LN) metastasis through the lymphatic network is a major route for cancer dissemination. Tumor cells reach the marginal sinuses of LNs via afferent lymphatic vessels (LVs) and form metastatic lesions that lead to distant metastasis. Thus, targeting of metastatic cells in the marginal sinuses could improve c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49386-5
更新日期：2019-09-13 00:00:00
abstract：:Whole-genome sequencing is likely to become increasingly used by local clinical microbiology laboratories, where sequencing volume is low compared with national reference laboratories. Here, we describe a universal protocol for simultaneous DNA extraction and sequencing of numerous different bacterial species, allowin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80031-8
更新日期：2021-01-08 00:00:00
abstract：:Amplification of liver injury is mediated by macrophages but the signaling by which the macrophage inflammasome enhances liver injury is not completely understood. The CCAAT/Enhancer Binding Protein-β (C/EBPβ) is a critical signaling molecule for macrophages because expression of a dominant inhibitor of C/EBPβ DNA-bin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24268
更新日期：2016-04-12 00:00:00
abstract：:Antigen (Ag)-mediated crosslinking of IgE-loaded high-affinity receptors for IgE (FcεRI) on mast cells (MCs) triggers activation of proinflammatory effector functions relevant for IgE-associated allergic disorders. The cytosolic tyrosine kinase BTK and the SH2-containing inositol-5'-phosphatase SHIP1 are central posit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33769-1
更新日期：2018-10-19 00:00:00
abstract：:It is common to find in the literature different values for the working voltage window (WVW) range for aqueous-based supercapacitors. In many cases, even with the best intentions of the widening the operating voltage window, the measured current using the cyclic voltammetry (CV) technique includes a significant contri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75851-7
更新日期：2020-11-05 00:00:00
abstract：:Caenorhabditis elegans presents functioning, biologically relevant phenotypes and is frequently used as a bioindicator of toxicity. However, most C. elegans in vivo effect-assessment methods are laborious and time consuming. Therefore, we developed a novel method to measure the oxygen consumption rate of C. elegans as...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61054-7
更新日期：2020-03-06 00:00:00
abstract：:Retinal diseases are frequently characterized by the accumulation of excessive scar tissue found throughout the neural retina. However, the pathophysiology of retinal fibrosis remains poorly understood, and the cell types that contribute to the fibrotic response are incompletely defined. Here, we show that myofibrobla...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72875-x
更新日期：2020-09-25 00:00:00
abstract：:The public goods game is a famous example illustrating the tragedy of the commons (Hardin in Science 162:1243-1248, 1968). In this game cooperating individuals contribute to a pool, which in turn is distributed to all members of the group, including defectors who reap the same rewards as cooperators without having mad...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79731-y
更新日期：2020-12-28 00:00:00
abstract：:Nonalcoholic fatty liver disease (NAFLD) is an increasing health problem worldwide, with genetic, epigenetic, and environmental components. Here, we describe the first example of NAFLD caused by genetic disruption of a mammalian potassium channel subunit. Mice with germline deletion of the KCNE2 potassium channel β su...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23118
更新日期：2016-03-17 00:00:00
abstract：:In January 2016, the Arctic experienced an extremely anomalous warming event after an extraordinary increase in air temperature at the end of 2015. During this event, a strong intrusion of warm and moist air and an increase in downward longwave radiation, as well as a loss of sea ice in the Barents and Kara seas, were...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40051
更新日期：2017-01-04 00:00:00
abstract：:Nitrogen metabolism in gut systems remains poorly studied in spite of its importance for microbial growth and its implications for the metabolism of the host. Prevotella spp. are the most predominant bacteria detected in the rumen, but their presence has also been related to health and disease states in the human gut ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08463-3
更新日期：2017-08-10 00:00:00
abstract：:In the current study, we aimed to investigate whether NADPH oxidase, a major ROS-producing enzyme, was involved in the antioxidant effect of acupuncture on cognitive impairment after cerebral ischaemia. The cognitive function, infract size, neuron cell loss, level of superoxide anion and expression of NADPH oxidase su...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17981
更新日期：2015-12-10 00:00:00
abstract：:Body mass index (BMI) is associated with increased future risk of inflammatory bowel disease(IBD) particularly Crohn's disease(CD), where associations with high and low BMI have been observed. Most studies are based on adult women. We aimed to explore the impact of BMI in men entering adult life on their long-term ris...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42642-8
更新日期：2019-04-23 00:00:00
abstract：:Following facial nerve axotomy, nerve function is not fully restored even after reconstruction. This may be attributed to axon degeneration/neuronal death and sustained neuroinflammation. CD38 is an enzyme that catalyses the hydrolysis of nicotinamide adenine dinucleotide (NAD+) and is a candidate molecule for regulat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73984-3
更新日期：2020-10-20 00:00:00
abstract：:This retrospective cohort study investigated the association between in-hospital survival and two-dimensional (2D) echocardiography within 24 hours after the return of spontaneous circulation (ROSC) in patients who underwent in-hospital cardiopulmonary resuscitation (ICPR) after in-hospital cardiopulmonary arrest (IHC...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56153-z
更新日期：2020-01-08 00:00:00
abstract：:This study investigates the efficacy of systemic growth hormone (GH) therapy in ameliorating the deleterious effects of chronic denervation (CD) injury on nerve regeneration and resulting motor function. Using a forelimb CD model, 4 groups of Lewis rats were examined (n = 8 per group): Group-1 (negative control) 8 wee...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39738-6
更新日期：2019-02-28 00:00:00
abstract：:Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical models. Since its disc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10238
更新日期：2015-05-19 00:00:00
abstract：:To assess the metabolically beneficial effects of fenugreek (Trigonella foenum-graecum), C57BL/6J mice were fed a low- or high-fat diet for 16 weeks with or without 2% (w/w) fenugreek supplementation. Body weight, body composition, energy expenditure, food intake, and insulin/glucose tolerance were measured regularly,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12846-x
更新日期：2017-10-06 00:00:00
abstract：:The renin-angiotensin system (RAS) plays a key role in the control of vasoconstriction as well as sodium and fluid retention mediated mainly by angiotensin (Ang) II acting at the AT1 receptor (AT1R). Ang-(1-7) is another RAS peptide, identified as the endogenous ligand of the Mas receptor and known to counterbalance m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12074-3
更新日期：2017-09-19 00:00:00
abstract：:3β-Hydroxysteroid-Δ24 reductase (DHCR24), the final enzyme of the cholesterol biosynthetic pathway, has been associated with urogenital neoplasms. However, the function of DHCR24 in endometrial cancer (EC) remains largely elusive. Here, we analyzed the expression profile of DHCR24 and the progesterone receptor (PGR) i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41404
更新日期：2017-01-23 00:00:00
abstract：:Surface plasmon polaritons (SPPs) are propagating excitations that arise from coupling of light with collective electron oscillations. Characterized by high field intensity and nanometric dimensions, SPPs fashion rapid expansion of interest from fundamental and applicative perspectives. However, high metallic losses a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17562
更新日期：2015-12-01 00:00:00
abstract：:Saccharina japonica is one of the most important marine crops in China, Japan and Korea. Candidate genes associated with blade length and blade width have not yet been reported. Here, based on SLAF-seq, the 7627 resulting SNP loci were selected for genetic linkage mapping to 31 linkage groups with an average spacing o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32015-y
更新日期：2018-09-11 00:00:00
abstract：:We investigated high-efficiency two-terminal tandem photovoltaic (PV) devices consisting of a p/i/n thin film silicon top sub-cell (p/i/n-TFS) and a heterojunction with an intrinsic thin-layer (HIT) bottom sub-cell. We used computer simulations and experimentation. The short-circuit current density (Jsc) of the top su...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33734-y
更新日期：2018-10-18 00:00:00
abstract：:We report on the fabrication of spherical Au spheres by pulsed laser treatment using a KrF excimer laser (248 nm, 25 ns) under ambient conditions as a fast and high throughput fabrication technique. The presented experiments were realized using initial Au layers of 100 nm thickness deposited on optically transparent a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29661-7
更新日期：2018-07-26 00:00:00
abstract：:Proton pump inhibitors (PPIs) belong to the most common medication in geriatric medicine. They are known to reduce osteoclast activity and to delay fracture healing in young adult mice. Because differentiation and proliferation in fracture healing as well as pharmacologic actions of drugs markedly differ in the elderl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79605-3
更新日期：2020-12-23 00:00:00
abstract：:We study the capillary retraction of a Newtonian semi-infinite liquid filament through analytical methods. We derive a long-time asymptotic-state expansion for the filament profile using a one-dimensional free-surface slender cylindrical flow model based on the three-dimensional axisymmetric Navier-Stokes equations. T...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51824-3
更新日期：2019-10-29 00:00:00
abstract：:We show that the space in which scientific, technological and economic activities interplay with each other can be mathematically shaped using techniques from statistical physics of networks. We build a holistic view of the innovation system as the tri-layered network of interactions among these many activities (scien...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52767-5
更新日期：2019-11-11 00:00:00
