Abstract:
:Receptor activator of nuclear factor-κB ligand (RANKL) is required for initiation of osteoclastogenesis, with the signaling pathway including the NF-kB, c-Fos, and nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) transcription factors. Because NFATc1 expression is autoamplified, we investigated the molecular mechanism by which peroxisome proliferator-activated receptor gamma (PPARγ) activation by the thiazolidinedione drug rosiglitazone decreases NFATc1 expression during RANKL stimulation. Western blotting demonstrated that rosiglitazone attenuated the increase in NFATc1 protein level induced by RANKL without affecting that of PPARγ. Immunofluorescence data indicated that rosiglitazone tended to suppress RANKL-induced NFATc1 nuclear translocation, partly by reducing calcineurin activity, as reflected by the observed decrease in nuclear NFATc1 abundance. On coimmunoprecipitation, the intensity of the physical interaction between NFATc1 and PPARγ was unexpectedly higher in the RANKL-stimulated group than in the control, but rosiglitazone reduced this to basal levels. Furthermore, RANKL failed to elevate mRNA expression of NFATc1 after PPARγ knockdown. ChIP assay indicated that rosiglitazone significantly reduced the binding of NFATc1 to its own promoter despite RANKL stimulation. These findings suggest that PPARγ activation by rosiglitazone blocks NFATc1 from binding to its own promoter, thereby reducing RANKL-induced NFATc1 autoamplification.
journal_name
FEBS Open Biojournal_title
FEBS open bioauthors
Park KL,Oh DG,Kim YO,Song KS,Ahn DWdoi
10.1002/2211-5463.12513subject
Has Abstractpub_date
2018-09-10 00:00:00pages
1584-1593issue
10issn
2211-5463pii
FEB412513journal_volume
8pub_type
杂志文章相关文献
FEBS Open Bio文献大全abstract::An imbalance of branched-chain amino acids (BCAAs) in the brain may result in neuropathological conditions, such as autism spectrum disorders. The L-type amino acid transporter 1 (LAT1), encoded by the solute carrier transporter 7a5 (Slc7a5) gene, is critical for maintaining normal levels of BCAAs in the brain. Howeve...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12559
更新日期:2019-01-07 00:00:00
abstract::Sorafenib, the first-line agent for treatment of advanced hepatocellular carcinoma (HCC), improves median overall survival by approximately 3 months. In the present study, we investigated whether sorafenib combined with cucurbitacin B (CuB), a natural tetracyclic triterpenoid isolated from Cucurbitaceae, exerts enhanc...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13035
更新日期:2020-11-11 00:00:00
abstract::Angiotensin converting enzyme (ACE) is a zinc-dependent dipeptidyl carboxypeptidase with an essential role in blood pressure homeostasis in mammals. ACE has long been targeted in the treatment of hypertension through ACE inhibitors, however current inhibitors are known to cause severe side effects. Therefore, there is...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.08.004
更新日期:2015-08-11 00:00:00
abstract::Ki-67 (MKI67) is a marker of cellular proliferation of cancer. Here, we show that Ki-67 is post-transcriptionally regulated through alternative polyadenylation (APA) and microRNAs in breast cancer. We show that shortening of the Ki-67 3'UTR results in the loss of the binding sites for the suppressive miRNAs and thus r...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12364
更新日期:2018-01-26 00:00:00
abstract::Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function. The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent. Only a few degradat...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12558
更新日期:2018-12-10 00:00:00
abstract::Thymic stromal lymphopoietin (TSLP) has been identified as a crucial inflammatory cytokine in immune homeostasis. Previous studies have reported conflicting effects of TSLP on sepsis in mice, and the effect of TSLP on sepsis in humans has not been investigated. In this study, we used the ELISA to measure serum levels ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12746
更新日期:2019-12-01 00:00:00
abstract::Microorganisms or LPS (lipopolysaccharide), an outer membrane component of Gram-negative bacteria, can induce a systemic inflammatory response that leads to sepsis, multiple organ dysfunction, and mortality. Here, we investigated the role of cyclophilin D (CypD)-dependent mitochondrial permeability transition (mPT) in...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13091
更新日期:2021-01-20 00:00:00
abstract::The extreme halophile Halococcus morrhuae (ATCC® 17082) contains a 108-nucleotide insertion in its 5S rRNA. Large rRNA expansions in Archaea are rare. This one almost doubles the length of the 5S rRNA. In order to understand how such an insertion is accommodated in the ribosome, we obtained a cryo-electron microscopy ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12962
更新日期:2020-10-01 00:00:00
abstract::In brain cells, glutamine transporters are vital to monitor and control the levels of glutamate and GABA. There are 11 members of the SLC38 family of amino acid transporters of which eight have been functionally characterized. Here, we report the first histological and functional characterization of the previously orp...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12219
更新日期:2017-04-26 00:00:00
abstract::Decorin (DCN) is a major member of the small leucine-rich proteoglycan (SLRP) family that is critically involved in tumorigenesis and the development of metastasis of cancers, including glioma. Overexpression of DCN was indicated to suppress glioma cell growth. However, the role of DCN in the migration of glioma cells...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12076
更新日期:2016-05-31 00:00:00
abstract::Eukaryotic protein O-mannosyltransferases (Pmts) are divided into three subfamilies (Pmt1, Pmt2, and Pmt4) and activity of Pmts in yeasts and animals requires assembly into complexes. In Saccharomyces cerevisiae, Pmt1 and Pmt2 form a heteromeric complex and Pmt 4 forms a homomeric complex. The filamentous fungus Asper...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.03.006
更新日期:2014-03-19 00:00:00
abstract::Zinc deficiency impairs the immune system leading to frequent infections. Although zinc is known to play critical roles in maintaining healthy immune function, the underlying molecular targets are largely unknown. In this study, we demonstrate that zinc is important for the CD154-CD40-mediated activation of downstream...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12211
更新日期:2017-03-27 00:00:00
abstract::Oxidized cholesterol (oxysterols) plays an important and multifaceted role in lipid metabolism. Here we examined whether dietary oxysterols accelerate hepatic lipid accumulation and inflammation in nonhuman primates. We also examined the effect of the Niemann-Pick C1-like1 inhibitor, ezetimibe (Ez). Macaca fasciculari...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12107
更新日期:2016-09-20 00:00:00
abstract::Hypoxia-inducible factor (HIF) is a crucial regulator of cellular and systemic responses to low oxygen levels. Here we firstly cloned three HIF-α isoforms from the basal branches of Osteichthyes and used computational tools to characterise the molecular change underlying the functional divergence of HIF-α isoforms in ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.09.004
更新日期:2013-09-19 00:00:00
abstract::Baicalin (5,6-dihydroxy-7-o-glucuronide flavone) is an extract from the roots of Chinese herb Huang Qin (Scutellaria baicalensis Georgi) and is reported to have antioxidative, antiproliferative, anti-inflammatory, and anticancer activities. This study aimed to investigate the inhibitory effect of baicalin on human hep...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12130
更新日期:2016-10-24 00:00:00
abstract::The identification of biomarkers for early diagnosis of Parkinson's disease (PD) prior to the onset of symptoms may improve the effectiveness of therapy. To identify potential biomarkers, we downloaded microarray datasets of PD from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between PD...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12687
更新日期:2019-08-01 00:00:00
abstract::The A/B domains of nuclear receptors such as thyroid receptor α (TRα) are considered to be conformationally flexible and can potentially adopt multiple structural conformations. We used intrinsic tryptophan fluorescence quenching and circular dichroism spectroscopy to characterize the unfolding of this A/B domain upon...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12229
更新日期:2017-05-15 00:00:00
abstract::Megakaryoblastic leukemia 1 (MKL1) is a member of the MKL family of serum response factor (SRF) coactivators. Here we have identified three rat MKL1 transcripts: two are homologues of mouse MKL1 transcripts, full-length MKL1 (FLMKL1) and basic, SAP, and coiled-coil domains (BSAC), the third is a novel transcript, MKL1...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2013.09.001
更新日期:2013-09-07 00:00:00
abstract::The antipsychotic drug olanzapine is widely prescribed to treat schizophrenia and other psychotic disorders. However, it often causes unwanted side effects, including diabetes, due to disruption of insulin-dependant glucose metabolism through a mechanism yet to be elucidated. To determine if olanzapine can affect the ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.04.006
更新日期:2015-04-15 00:00:00
abstract::Memory includes the processes of acquisition, consolidation and retrieval. In the study of aversive olfactory memory in Drosophila melanogaster, flies are first exposed to an odor (conditioned stimulus, CS+) that is associated with an electric shock (unconditioned stimulus, US), then to another odor (CS-) without the ...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12203
更新日期:2017-02-22 00:00:00
abstract::Flavin adenine dinucleotide (FAD), synthesized from riboflavin, is redox cofactor in energy production and plays an important role in cell survival. More recently, riboflavin deficiency has been linked to developmental disorders, but its role in stem cell differentiation remains unclear. Here, we show that FAD treatme...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12331
更新日期:2017-10-17 00:00:00
abstract::Flavonoids represent one of the most important molecules of plant secondary metabolism, playing many different biochemical and physiological roles. Although their essential role in plant life and human health has been elucidated by many studies, their subcellular transport and accumulation in plant tissues remains unc...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2015.08.009
更新日期:2015-08-29 00:00:00
abstract::Osteosarcoma is a malignant bone tumor, and clinically detectable metastases can be detected in ~ 15-20% of patients when they seek medical advice; patients with metastatic disease have extremely poor prognosis. Here, we examined the involvement of the microRNA miR-505 in osteosarcoma. Eighty-four patients seeking tre...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12868
更新日期:2020-07-01 00:00:00
abstract::Due to its exceptionally small genome size and protogynous hermaphroditism, Monopterus albus has been proposed as a model for vertebrate sexual development. The Kiss/GPR54 system is a central regulator of sexual development in most vertebrates, but its role in sex reversal remains hypothetical. In contrast to mammals,...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12727
更新日期:2019-10-01 00:00:00
abstract::The tumour microenvironment comprises a diverse range of cells, including fibroblasts, immune cells and endothelial cells, along with extracellular matrix. In particular, fibroblasts are of significant interest as these cells are reprogrammed during tumorigenesis to become cancer-associated fibroblasts (CAFs), which i...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12831
更新日期:2020-05-01 00:00:00
abstract::In this study, it was shown for the first time that l-amino acid oxidase of Pseudomonas sp. AIU813, renamed as l-amino acid oxidase/monooxygenase (l-AAO/MOG), exhibits l-lysine 2-monooxygenase as well as oxidase activity. l-Lysine oxidase activity of l-AAO/MOG was increased in a p-chloromercuribenzoate (p-CMB) concent...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1016/j.fob.2014.02.002
更新日期:2014-02-07 00:00:00
abstract::Candida albicans is a harmless commensal resident in the human gut, and a prevalent opportunistic pathogen. A key part of its commensalism and pathogenesis is its ability to switch between different morphological forms, including white-to-opaque switching. The Wor1 protein was previously identified as a master regulat...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.13070
更新日期:2020-12-22 00:00:00
abstract::Colorectal cancer (CRC) is the third main cause of cancer-relevant deaths worldwide, and its incidence has increased in recent decades. Previous studies have indicated that certain long non-coding RNAs (lncRNAs) have regulatory roles in tumor occurrence and progression. Often, lncRNAs are competitive endogenous RNAs (...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12802
更新日期:2020-01-27 00:00:00
abstract::Dihydroorotate dehydrogenase (DHODH) is a flavin-binding enzyme essential for pyrimidine biosynthesis, which converts dihydroorotate to orotate. Three-dimensional structures of cytosolic DHODH of parasitic protozoa are of interest in drug discovery for neglected tropical diseases, especially because these enzymes poss...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12403
更新日期:2018-03-06 00:00:00
abstract::As a small fruit rich in anthocyanins, blueberry (Vaccinium corymbosum × angustifolium) has become a focus of research in recent years for identifying genes related to anthocyanin transport and stress resistance mechanisms based on transcriptome sequencing. However, the lack of validated, stably expressed reference ge...
journal_title:FEBS open bio
pub_type: 杂志文章
doi:10.1002/2211-5463.12903
更新日期:2020-08-01 00:00:00