Abstract:
:The in vitro expression of two distinct proteins from overlapping reading frames in a sequence of rainbow trout genomic DNA has been demonstrated. In vitro transcription of DNA sequences, cloned in a plasmid under the control of Salmonella phage 6 polymerase promoter, led to the synthesis of two distinct and functional mRNAs corresponding to the protamine mRNA and also to another overlapping mRNA, termed Y. These mRNAs were translated in an mRNA-dependent rabbit reticulocyte lysate cell free system which synthesized the corresponding protein products. Similarities between the synthesized Pro-rich protein Y and three proline-rich proteins, the human salivary Pro-rich protein, the avian sarcoma virus protein P19 and the myc oncogene product, were evident and the significance of these findings is discussed. A synthetic oligonucleotide which is complementary to a sequence corresponding to a region of the Y protein mRNA, but upstream (5') of the transcribed protamine mRNA, hybridized faintly and only to trout brain RNA. However, more sensitive primer extension studies utilizing the Y-specific oligonucleotide detected several Y-related mRNAs in trout brain.
journal_name
J Mol Evoljournal_title
Journal of molecular evolutionauthors
Jankowski JM,Krawetz SA,Walczyk E,Dixon GHdoi
10.1007/BF02099952subject
Has Abstractpub_date
1986-01-01 00:00:00pages
61-71issue
1-2eissn
0022-2844issn
1432-1432journal_volume
24pub_type
杂志文章abstract::MicroRNAs (miRNAs) are small noncoding RNA molecules which are processed into ~20-24 nt molecules that can regulate the gene expression post-transcriptionally. MiRNA gene clusters have been identified in a range of species, where in miRNAs are often processed from polycistronic transcripts. In this study, a computatio...
journal_title:Journal of molecular evolution
pub_type: 信件
doi:10.1007/s00239-013-9563-2
更新日期:2013-06-01 00:00:00
abstract::Amino acid substitution models represent substitution rates among amino acids during the evolution. The models play an important role in analyzing protein sequences, especially inferring phylogenies. The rapid evolution of flaviviruses is expanding the threat in public health. A number of models have been estimated fo...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-020-09943-3
更新日期:2020-07-01 00:00:00
abstract::As illustrated by the mitochondrion and the eukaryotic cell, little in biology makes sense except in light of mutualism. Mutualisms are persistent, intimate, and reciprocal exchanges; an organism proficient in obtaining certain benefits confers those on a partner, which reciprocates by conferring different benefits. M...
journal_title:Journal of molecular evolution
pub_type: 杂志文章,评审
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更新日期:2017-08-01 00:00:00
abstract::The present work represents the first attempt to study in greater detail previously proposed compositional correlations in genomes, based on a body of additional data relating to gene localizations as well as to extended flanking sequences extracted from gene banks. We have investigated the correlations that exist bet...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02102651
更新日期:1991-06-01 00:00:00
abstract::Hypothetical Products from Noncoding Frames (i.e., HyPNoFs) are hypothetical, not-coded proteins, translated from alternate reading frames (i.e., coding + 1 and coding + 2) of cDNAs. HyPNoFs of CD4, PKC, oncostatin, bcl-2 proto-oncogene, tumor suppressor p53, cystic fibrosis transmembrane regulator (CFTR), and tumor n...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF00160503
更新日期:1995-06-01 00:00:00
abstract::Available molecular and biochemical data offer conflicting evidence for the origin of the cyanelle of Cyanophora paradoxa. We show that the similarity of cyanelle and green chloroplast sequences is probably a result of these two lineages independently developing the same pattern of directional nucleotide change (subst...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF00182392
更新日期:1992-02-01 00:00:00
abstract::We have studied the relative contribution of inversions, transpositions, deletions, and nucleotide substitutions to the evolution of Chlamydia trachomatis and Chlamydia pneumoniae. The minimal number of rearrangement events required for converting the gene order structure of one genome into that of the other was estim...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-001-0087-9
更新日期:2002-07-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02198833
更新日期:1996-02-01 00:00:00
abstract::Orphan genes, lacking detectable homologs in outgroup species, typically represent 10-30% of eukaryotic genomes. Efforts to find the source of these young genes indicate that de novo emergence from non-coding DNA may in part explain their prevalence. Here, we investigate the roots of orphan gene emergence in the Droso...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-020-09939-z
更新日期:2020-05-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-014-9620-5
更新日期:2014-05-01 00:00:00
abstract::Insertions and deletions of entire codons have recently been discovered as a mechanism by which B cells, in addition to conventional base substitution, evolve the antibodies produced by their immunoglobulin genes. These events frequently seem to involve repetitive sequence motifs in the antibody-encoding genes, and it...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-001-0049-2
更新日期:2002-03-01 00:00:00
abstract::By a chromosome walking strategy the DNA region from Methanococcus vannielii flanking the genes for protein synthesis elongation factor (EF) 1 alpha and EF-2 was cloned and sequenced. A gene organization of 5' - beta' - open reading frame (ORF) 1 - ORF2 - S12 - S7 - EF-2 - EF-1 alpha - S10 - ORF3 - ORF4 - 3' was found...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02106178
更新日期:1989-07-01 00:00:00
abstract::Previous observations have indicated that Drosophila DNA contains a component that evolves so rapidly that it fails to hybridize between the DNAs of sibling species. To establish the reality of this component and study its properties, the fraction (about 20%) of Drosophila simulans (Dsim) DNA that fails to hybridize t...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02099998
更新日期:1990-03-01 00:00:00
abstract::Eight human short tandem repeat polymorphisms (STRs) also known as microsatellites-DYS19, DYS388, DYS390, DYS391, DYS392, DYS393, DYS389I, and DYS389II, mapping in the Y chromosome-were analyzed in two Iberian samples (Basques and Catalans). Allele frequency distributions showed significant differences only for DYS392...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/pl00006229
更新日期:1997-09-01 00:00:00
abstract::Actin is a highly conserved protein although many isoforms exist. In vertebrates and insects the different actin isoforms can be grouped by their amino acid sequence and tissue-specific gene expression into muscle and nonmuscle actins, suggesting that the different actins may have a functional significance. We ask her...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/pl00006125
更新日期:1997-01-01 00:00:00
abstract::The complete mitochondrial DNA (mtDNA) sequence of the brine shrimp Artemia franciscana has been determined. It extends the present knowledge of mitochondrial genomes to the crustacean class and supplies molecular markers for future comparative studies in this large branch of the arthropod phylum. Artemia mtDNA is 15,...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF00160272
更新日期:1994-10-01 00:00:00
abstract::The mitochondrial cytochrome b (cyt-b) gene is widely used in systematic studies to resolve divergences at many taxonomic levels. The present study focuses mainly on the utility of cyt-b as a molecular marker for inferring phylogenetic relationship at various levels within the fish family Cichlidae. A total of 78 taxa...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s002390010197
更新日期:2001-08-01 00:00:00
abstract::The finding that ribozymes can catalyze RNA chain elongation has led to the proposal that an early self-replicating system could have consisted of RNA alone. In such a chain elongation reaction, the Tetrahymena ribozyme was found to select 3',5'-linked substrates from a pool that contained a large molar excess of 2',5...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02602919
更新日期:1989-12-01 00:00:00
abstract::We examined the cis- vs. trans-splicing status of the mitochondrial group II intron nad1i728 in 439 species (427 genera) of land plants, using both Southern hybridization results (for 416 species) and intron sequence data from the literature. A total of 164 species (157 genera), all angiosperms, was found to have a tr...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-004-2606-y
更新日期:2004-07-01 00:00:00
abstract::Transfer RNA (tRNA) is the central intellectual property in the evolution of life on Earth. tRNA evolved from repeats and inverted repeats of known sequence. The anticodon and the T stem-loop-stems are homologs with significant conserved sequence identity. A number of models have been advanced to explain tRNA evolutio...
journal_title:Journal of molecular evolution
pub_type: 信件
doi:10.1007/s00239-020-09928-2
更新日期:2020-04-01 00:00:00
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journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-002-2335-z
更新日期:2002-10-01 00:00:00
abstract::Oxygen-free aqueous solutions of NH4CN (0.1 M, pH9) were exposed to gamma rays from a 60Co source, the mixture of nonvolatile products was fractionated, and the fractions were analyzed. The procedures were chosen to make effective investigations of radiolytic products, and to minimize the contributions of chemical cha...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF01732951
更新日期:1980-07-01 00:00:00
abstract::Previous reports indicate that in laboratory strains of mice, males are distinct from females in possession of repetitive DNA, notably devoid of Eco RI and Hae III sites and rich in the simple tetranucleotides GATA/GACA. We report here that such sequences originated in an ancestor common to laboratory mice, Mus hortul...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02100013
更新日期:1987-01-01 00:00:00
abstract::The attachment site (attlambda) of bacteriophage lambda was examined in wild strains of Escherichia coli. Although the att region is non-coding, the DNA sequence was invariant in the 13 strains examined. Two other non-coding regions showed nine changes, all associated with a single strain. In four of 33 strains, seque...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s002390010247
更新日期:2001-12-01 00:00:00
abstract::In bacteria, synonymous codon usage can be considerably affected by base composition at neighboring sites. Such context-dependent biases may be caused by either selection against specific nucleotide motifs or context-dependent mutation biases. Here we consider the evolutionary conservation of context-dependent codon b...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s002399910031
更新日期:2000-03-01 00:00:00
abstract::Universal scale of the sequence conservation has been recently introduced based on omnipresence of the protein sequence motifs across species. A large spectrum of short sequences, up to eight residues has been found to reside in all or almost all prokaryotic organisms. By this discovery a principally novel quantitativ...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-005-0190-4
更新日期:2006-11-01 00:00:00
abstract::We have used three reference sequences representative of bacterial drug resistance pumps and sugar transport proteins to collect the 91 most closely related sequences from a composite, nonredundant protein sequence database. Having eliminated certain very close relatives, the remainder were subjected to analysis and a...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/BF02198855
更新日期:1996-02-01 00:00:00
abstract::In mammals, chicken-type (c-type) lysozymes are part of the innate immune system, killing bacteria by degrading peptidoglycan in their cell walls. Many of the studies on the evolution of c-type lysozymes have focused on its new digestive function, including the duplicated stomach lysozymes in ruminants. Similarly, in ...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s00239-019-09910-7
更新日期:2019-12-01 00:00:00
abstract::Fimbrial adhesins allow bacteria to interact with and attach to their environment. The bacteria possibly benefit from these interactions, but all external structures including adhesins also allow bacteria to be identified by other organisms. Thus adhesion molecules might be under multiple forms of selection including ...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/s002390010148
更新日期:2001-02-01 00:00:00
abstract::Multiple phospholipase A2 (PLA2) isoenzymes found in a single snake venom induce a variety of pharmacological effects. These multiple forms are formed by gene duplication and accelerated evolution of exons. We examined the amino acid sequences of 127 snake venom PLA2 enzymes and their homologues to study in which loca...
journal_title:Journal of molecular evolution
pub_type: 杂志文章
doi:10.1007/pl00006450
更新日期:1999-02-01 00:00:00