Photothermal Off-Resonance Tapping for Rapid and Gentle Atomic Force Imaging of Live Cells.


:Imaging living cells by atomic force microscopy (AFM) promises not only high-resolution topographical data, but additionally, mechanical contrast, both of which are not obtainable with other microscopy techniques. Such imaging is however challenging, as cells need to be measured with low interaction forces to prevent either deformation or detachment from the surface. Off-resonance modes which periodically probe the surface have been shown to be advantageous, as they provide excellent force control combined with large amplitudes, which help reduce lateral force interactions. However, the low actuation frequency in traditional off-resonance techniques limits the imaging speed significantly. Using photothermal actuation, we probe the surface by directly actuating the cantilever. Due to the much smaller mass that needs to be actuated, the achievable measurement frequency is increased by two orders of magnitude. Additionally, photothermal off-resonance tapping (PORT) retains the precise force control of conventional off-resonance modes and is therefore well suited to gentle imaging. Here, we show how photothermal off-resonance tapping can be used to study live cells by AFM. As an example of imaging mammalian cells, the initial attachment, as well as long-term detachment, of human thrombocytes is presented. The membrane disrupting effect of the antimicrobial peptide CM-15 is shown on the cell wall of Escherichia coli. Finally, the dissolution of the cell wall of Bacillus subtilis by lysozyme is shown. Taken together, these evolutionarily disparate forms of life exemplify the usefulness of PORT for live cell imaging in a multitude of biological disciplines.


Int J Mol Sci


Nievergelt AP,Brillard C,Eskandarian HA,McKinney JD,Fantner GE




Has Abstract


2018-09-30 00:00:00










  • Melanogenesis-inducing effect of cirsimaritin through increases in microphthalmia-associated transcription factor and tyrosinase expression.

    abstract::The melanin-inducing properties of cirsimaritin were investigated in murine B16F10 cells. Cirsimaritin is an active flavone with methoxy groups, which is isolated from the branches of Lithocarpus dealbatus. Tyrosinase activity and melanin content in murine B16F10 melanoma cells were increased by cirsimaritin in a dose...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Kim HJ,Kim IS,Dong Y,Lee IS,Kim JS,Kim JS,Woo JT,Cha BY

    更新日期:2015-04-20 00:00:00

  • Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase.

    abstract::Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as Sphingobium sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed ba...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Jia Y,Eltoukhy A,Wang J,Li X,Hlaing TS,Aung MM,Nwe MT,Lamraoui I,Yan Y

    更新日期:2020-05-19 00:00:00

  • Berry phenolics of grapevine under challenging environments.

    abstract::Plant phenolics have been for many years a theme of major scientific and applied interest. Grape berry phenolics contribute to organoleptic properties, color and protection against environmental challenges. Climate change has already caused significant warming in most grape-growing areas of the world, and the climatic...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Teixeira A,Eiras-Dias J,Castellarin SD,Gerós H

    更新日期:2013-09-11 00:00:00

  • Vasoprotective Functions of High-Density Lipoproteins Relevant to Alzheimer's Disease Are Partially Conserved in Apolipoprotein B-Depleted Plasma.

    abstract::High-density lipoproteins (HDL) are known to have vasoprotective functions in peripheral arteries and many of these functions extend to brain-derived endothelial cells. Importantly, several novel brain-relevant HDL functions have been discovered using brain endothelial cells and in 3D bioengineered human arteries. The...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Button EB,Gilmour M,Cheema HK,Martin EM,Agbay A,Robert J,Wellington CL

    更新日期:2019-01-22 00:00:00

  • Physicochemical and Antimicrobial Characterization of Beeswax-Starch Food-Grade Nanoemulsions Incorporating Natural Antimicrobials.

    abstract::Nanoemulsions are feasible delivery systems of lipophilic compounds, showing potential as edible coatings with enhanced functional properties. The aim of this work was to study the effect of emulsifier type (stearic acid (SA), Tween 80 (T80) or Tween 80/Span 60 (T80/S60)) and emulsification process (homogenization, ul...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Arredondo-Ochoa T,García-Almendárez BE,Escamilla-García M,Martín-Belloso O,Rossi-Márquez G,Medina-Torres L,Regalado-González C

    更新日期:2017-12-15 00:00:00

  • Clinical Response to Personalized Exercise Therapy in Heart Failure Patients with Reduced Ejection Fraction is Accompanied by Skeletal Muscle Histological Alterations.

    abstract::Abstract: Heart failure (HF) is associated with skeletal muscle wasting and exercise intolerance. This study aimed to evaluate the exercise-induced clinical response and histological alterations. One hundred and forty-four HF patients were enrolled. The individual training program was determined as a workload at or cl...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lelyavina TA,Galenko VL,Ivanova OA,Komarova MY,Ignatieva EV,Bortsova MA,Yukina GY,Khromova NV,Sitnikova MY,Kostareva AA,Sergushichev A,Dmitrieva RI

    更新日期:2019-11-05 00:00:00

  • Natural Bioactive Compounds: The Way Shown by Professor Maurizio Battino and His Group in an Italian Cutting-Edge Laboratory.

    abstract::Maurizio Battino, Ph.D., Associate Professor of Biochemistry in the Department of Clinical Sciences, Faculty of Medicine, Università Politecnica delle Marche (Italy), is the Director of the Centre for Health and Nutrition, Universidad Europea del Atlantico (Santander, Spain) and Director of Nutrition and Health projec...

    journal_title:International journal of molecular sciences

    pub_type: 面试


    authors: Editorial Office International Journal Of Molecular Sciences.

    更新日期:2016-07-05 00:00:00

  • Spike Activator 1, Encoding a bHLH, Mediates Axillary Bud Development and Spike Initiation in Phalaenopsis aphrodite.

    abstract::Double-spikes Phalaenopsis orchids have greater market value than those with single-spike. In this study, a gene designated as Spike Activator 1 (SPK1), which encodes a basic helix-loop-helix (bHLH) transcription factor, was isolated and characterized from Phalaenopsis aphrodite (moth orchid). SPK1 was highly expresse...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lin YJ,Li MJ,Hsing HC,Chen TK,Yang TT,Ko SS

    更新日期:2019-10-30 00:00:00

  • Radioprotective Effects of Dermatan Sulfate in a Preclinical Model of Oral Mucositis-Targeting Inflammation, Hypoxia and Junction Proteins without Stimulating Proliferation.

    abstract::Oral mucositis is the most frequently occurring early side effect of head-and-neck cancer radiotherapy. Systemic dermatan sulfate (DS) treatment revealed a significant radioprotective potential in a preclinical model of oral mucositis. This study was initiated to elucidate the mechanistic effects of DS in the same mod...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Gruber S,Arnold M,Cini N,Gernedl V,Hetzendorfer S,Kowald LM,Kuess P,Mayer J,Morava S,Pfaffinger S,Rohorzka A,Dörr W

    更新日期:2018-06-06 00:00:00

  • Protein Recognition in Drug-Induced DNA Alkylation: When the Moonlight Protein GAPDH Meets S23906-1/DNA Minor Groove Adducts.

    abstract::DNA alkylating drugs have been used in clinics for more than seventy years. The diversity of their mechanism of action (major/minor groove; mono-/bis-alkylation; intra-/inter-strand crosslinks; DNA stabilization/destabilization, etc.) has undoubtedly major consequences on the cellular response to treatment. The aim of...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Savreux-Lenglet G,Depauw S,David-Cordonnier MH

    更新日期:2015-11-05 00:00:00

  • Chronic Kidney Disease and Exposure to Nephrotoxic Metals.

    abstract::Chronic kidney disease (CKD) is a common progressive disease that is typically characterized by the permanent loss of functional nephrons. As injured nephrons become sclerotic and die, the remaining healthy nephrons undergo numerous structural, molecular, and functional changes in an attempt to compensate for the loss...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Orr SE,Bridges CC

    更新日期:2017-05-12 00:00:00

  • Estrogen Abolishes the Repression Role of gga-miR-221-5p Targeting ELOVL6 and SQLE to Promote Lipid Synthesis in Chicken Liver.

    abstract::Few studies have been conducted regarding the biological function and regulation role of gga-miR-221-5p in the liver. We compared the conservation of miR-221-5p among species and investigated the expression pattern of gga-miR-221-5p, validating the direct target genes of gga-miR-221-5p by dual luciferase reporter assa...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zhang DD,Wang DD,Wang Z,Wang YB,Li GX,Sun GR,Tian YD,Han RL,Li ZJ,Jiang RR,Liu XJ,Kang XT,Li H

    更新日期:2020-02-27 00:00:00

  • The Dark Side of the Force: When the Immune System Is the Fuel of Tumor Onset.

    abstract::Nowadays, it is well accepted that inflammation is a critical player in cancer, being, in most cases, the main character of the process. Different types of tumor arise from sites of infection or chronic inflammation. This non-resolving inflammation is responsible for tumor development at different levels: it promotes ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Digifico E,Balinzo S,Belgiovine C

    更新日期:2021-01-27 00:00:00

  • Theoretical and experimental spectroscopic analysis of cyano-substituted styrylpyridine compounds.

    abstract::A combined theoretical and experimental study on the structure, infrared, UV-Vis and 1H NMR data of trans-2-(m-cyanostyryl)pyridine, trans-2-[3-methyl-(m-cyanostyryl)]pyridine and trans-4-(m-cyanostyryl)pyridine is presented. The synthesis was carried out with an efficient Knoevenagel condensation using green chemist...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Castro ME,Percino MJ,Chapela VM,Ceron M,Soriano-Moro G,Lopez-Cruz J,Melendez FJ

    更新日期:2013-02-18 00:00:00

  • Exceptionally Long-Lived Individuals (ELLI) Demonstrate Slower Aging Rate Calculated by DNA Methylation Clocks as Possible Modulators for Healthy Longevity.

    abstract::Exceptionally long-lived individuals (ELLI) who are the focus of many healthy longevity studies around the globe are now being studied in Israel. The Israeli Multi-Ethnic Centenarian Study (IMECS) cohort is utilized here for assessment of various DNA methylation clocks. Thorough phenotypic characterization and whole b...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Gutman D,Rivkin E,Fadida A,Sharvit L,Hermush V,Rubin E,Kirshner D,Sabin I,Dwolatzky T,Atzmon G

    更新日期:2020-01-17 00:00:00

  • Revealing and Harnessing Tumour-Associated Microglia/Macrophage Heterogeneity in Glioblastoma.

    abstract::Abstract: Cancer heterogeneity and progression are subject to complex interactions between neoplastic cells and their microenvironment, including the immune system. Although glioblastomas (GBMs) are classified as 'cold tumours' with very little lymphocyte infiltration, they can contain up to 30-40% of tumour-associate...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Pires-Afonso Y,Niclou SP,Michelucci A

    更新日期:2020-01-21 00:00:00

  • The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins-Metal Complexes.

    abstract::The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behav...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Merlos Rodrigo MA,Molina-López J,Jimenez Jimenez AM,Planells Del Pozo E,Adam P,Eckschlager T,Zitka O,Richtera L,Adam V

    更新日期:2017-03-11 00:00:00

  • Involvement of Satellite Cell Activation via Nitric Oxide Signaling in Ectopic Orofacial Hypersensitivity.

    abstract::: The mechanical head-withdrawal threshold (MHWT) was significantly reduced following inferior alveolar nerve transection (IANX) in rats. Nitrate and nitrite synthesis was dramatically increased in the trigeminal ganglion (TG) at 6 h after the IANX. The relative number of neuronal nitric oxide synthase (nNOS)-immunore...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lee J,Ohara K,Shinoda M,Hayashi Y,Kubo A,Sugawara S,Asano S,Soma K,Kanno K,Ando M,Koyama R,Kimura Y,Sakanashi K,Iinuma T,Iwata K

    更新日期:2020-02-13 00:00:00

  • Reactive oxygen species released from hypoxic hepatocytes regulates MMP-2 expression in hepatic stellate cells.

    abstract::Hypoxia is a common environmental stress factor and is associated with fibrogenesis. Matrix metalloproteinase-2 (MMP-2), produced by hepatic stellate cells (HSCs), plays an important role in liver fibrogenesis. However, inconsistent results have been reported on the impact of hypoxia on MMP-2 expression and activity i...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Li J,Fan R,Zhao S,Liu L,Guo S,Wu N,Zhang W,Chen P

    更新日期:2011-01-01 00:00:00

  • Effect of Curcumin on Protein Damage Induced by Rotenone in Dopaminergic PC12 Cells.

    abstract::Oxidative stress is considered to be a key factor of the pathogenesis of Parkinson's disease, a multifactorial neurodegenerative disorder characterized by reduced dopaminergic neurons in the substantia nigra pars compacta and accumulated protein aggregates. Rotenone is a worldwide-used pesticide that induces the most ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Buratta S,Chiaradia E,Tognoloni A,Gambelunghe A,Meschini C,Palmieri L,Muzi G,Urbanelli L,Emiliani C,Tancini B

    更新日期:2020-04-16 00:00:00

  • Computer-Aided Estimation of Biological Activity Profiles of Drug-Like Compounds Taking into Account Their Metabolism in Human Body.

    abstract::Most pharmaceutical substances interact with several or even many molecular targets in the organism, determining the complex profiles of their biological activity. Moreover, due to biotransformation in the human body, they form one or several metabolites with different biological activity profiles. Therefore, the deve...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Filimonov DA,Rudik AV,Dmitriev AV,Poroikov VV

    更新日期:2020-10-11 00:00:00

  • The Direct Interaction between E93 and Kr-h1 Mediated Their Antagonistic Effect on Ovary Development of the Brown Planthopper.

    abstract::The juvenile hormone (JH) signalling and ecdysone signalling pathways are crucial endocrine signalling pathways that orchestrate the metamorphosis of insects. The metamorphic process, the morphological change from the immature to adult forms, is orchestrated by the dramatic reduction of JH and downstream transcription...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Mao Y,Li Y,Gao H,Lin X

    更新日期:2019-05-16 00:00:00

  • Reaction with Proteins of a Five-Coordinate Platinum(II) Compound.

    abstract::Stable five-coordinate Pt(II) complexes have been highlighted as a promising and original platform for the development of new cytotoxic drugs. Their interaction with proteins has been scarcely studied. Here, the reactivity of the five-coordinate Pt(II) compound [Pt(I)(Me) (dmphen)(olefin)] (Me = methyl, dmphen = 2,9-d...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ferraro G,Marzo T,Cucciolito ME,Ruffo F,Messori L,Merlino A

    更新日期:2019-01-26 00:00:00

  • Cisplatin Synergistically Enhances Antitumor Potency of Conditionally Replicating Adenovirus via p53 Dependent or Independent Pathways in Human Lung Carcinoma.

    abstract::Cisplatin is ranked as one of the most powerful and commonly prescribed anti-tumor chemotherapeutic agents which improve survival in many solid tumors including non-small cell lung cancer. However, the treatment of advanced lung cancer is restricted due to chemotherapy resistance. Here, we developed and investigated s...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ali S,Tahir M,Khan AA,Chen XC,Ling M,Huang Y

    更新日期:2019-03-05 00:00:00

  • Multidisciplinary Treatment, Including Locoregional Chemotherapy, for Merkel-Polyomavirus-Positive Merkel Cell Carcinomas: Perspectives for Patients Exhibiting Oncogenic Alternative Δ exon 6-7 TrkAIII Splicing of Neurotrophin Receptor Tropomyosin-Related

    abstract::Merkel cell carcinomas (MCCs) are rare, aggressive, cutaneous neuroendocrine tumours, approximately 80% of which are caused by the genomic integration of Merkel cell polyomavirus (MCPyV). MCPyV-positive MCCs carry poor prognosis in approximately 70% of cases, highlighting the need for greater understanding of the onco...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Guadagni S,Farina AR,Cappabianca LA,Sebastiano M,Maccarone R,Zelli V,Clementi M,Chiominto A,Bruera G,Ricevuto E,Fiorentini G,Sarti D,Mackay AR

    更新日期:2020-11-03 00:00:00

  • The effect of sericin from various extraction methods on cell viability and collagen production.

    abstract::Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential, particle size and a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Aramwit P,Kanokpanont S,Nakpheng T,Srichana T

    更新日期:2010-05-20 00:00:00

  • Different impacts of cardiovascular risk factors on oxidative stress.

    abstract::The objective of the study was to evaluate oxidative stress (OS) status in subjects with different cardiovascular risk factors. With this in mind, we have studied three models of high cardiovascular risk: hypertension (HT) with and without metabolic syndrome, familial hypercholesterolemia (FH) and familial combined hy...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Mansego ML,Redon J,Martinez-Hervas S,Real JT,Martinez F,Blesa S,Gonzalez-Albert V,Saez GT,Carmena R,Chaves FJ

    更新日期:2011-01-01 00:00:00

  • The Impact of Genetic Polymorphisms in Organic Cation Transporters on Renal Drug Disposition.

    abstract::A considerable number of drugs and/or their metabolites are excreted by the kidneys through glomerular filtration and active renal tubule secretion via transporter proteins. Uptake transporters in the proximal tubule are part of the solute carrier (SLC) superfamily, and include the organic cation transporters (OCTs). ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Zazuli Z,Duin NJCB,Jansen K,Vijverberg SJH,Maitland-van der Zee AH,Masereeuw R

    更新日期:2020-09-10 00:00:00

  • Modulatory Effect of Myokines on Reactive Oxygen Species in Ischemia/Reperfusion.

    abstract::There is a growing body of evidence showing the importance of physical activity against acute ischemic events in various organs. Ischemia/reperfusion injury (I/R) is characterized by tissue damage as a result of restriction and subsequent restoration of blood supply to an organ. Oxidative stress due to increased react...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Szabó MR,Pipicz M,Csont T,Csonka C

    更新日期:2020-12-09 00:00:00

  • Metabolomic and Transcriptomic Profiling Provide Novel Insights into Fruit Ripening and Ripening Disorder Caused by 1-MCP Treatments in Papaya.

    abstract::Treatment with 1-methylcyclopropylene (1-MCP) is an effective technique to preserve fruits, but inappropriate treatment with 1-MCP causes a ripening disorder (rubbery texture) in papaya fruit. In this study, a combined metabolomic and transcriptomic analysis was conducted to reveal the possible mechanism of the ripeni...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zheng S,Hao Y,Fan S,Cai J,Chen W,Li X,Zhu X

    更新日期:2021-01-18 00:00:00