Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project.

Abstract:

:Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies.

journal_name

J Breath Res

authors

Ahmed WM,Brinkman P,Weda H,Knobel HH,Xu Y,Nijsen TM,Goodacre R,Rattray N,Vink TJ,Santonico M,Pennazza G,Montuschi P,Sterk PJ,Fowler SJ

doi

10.1088/1752-7163/aae557

subject

Has Abstract

pub_date

2018-10-30 00:00:00

pages

016001

issue

1

eissn

1752-7155

issn

1752-7163

journal_volume

13

pub_type

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