Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project.


:Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies.


J Breath Res


Ahmed WM,Brinkman P,Weda H,Knobel HH,Xu Y,Nijsen TM,Goodacre R,Rattray N,Vink TJ,Santonico M,Pennazza G,Montuschi P,Sterk PJ,Fowler SJ




Has Abstract


2018-10-30 00:00:00












  • Measurement of exhaled volatile organic compounds from patients with chronic obstructive pulmonary disease (COPD) using closed gas loop GC-IMS and GC-APCI-MS.

    abstract::Due to its high sensitivity, compact size and low cost ion mobility spectrometry (IMS) has the potential to become a point-of-care breath analyzer. Therefore, we developed a prototype of a compact, closed gas loop IMS with gas chromatographic (GC) pre-separation and high resolving power of R  =  90. In this study, we ...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Allers M,Langejuergen J,Gaida A,Holz O,Schuchardt S,Hohlfeld JM,Zimmermann S

    更新日期:2016-04-08 00:00:00

  • Endogenous volatile organic compounds in breath and blood of healthy volunteers: examining breath analysis as a surrogate for blood measurements.

    abstract::To investigate the premise that levels of endogenous volatile organic compounds (VOC) in breath reflect those in blood, the concentration of acetone and isoprene were measured in radial arterial blood, peripheral venous blood and breath samples from ten healthy volunteers. Coefficients of repeatability as a percentage...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: O'Hara ME,Clutton-Brock TH,Green S,Mayhew CA

    更新日期:2009-06-01 00:00:00

  • Implementation of Fowler's method for end-tidal air sampling.

    abstract::The design, realization and testing of a CO(2)-triggered breath sampler, capable of a separate collection of dead space and end-tidal air on multiple breaths, is presented. This sampling procedure has advantages in terms of the sample volume, insights regarding the origin of compounds, increased reproducibility and hi...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Di Francesco F,Loccioni C,Fioravanti M,Russo A,Pioggia G,Ferro M,Roehrer I,Tabucchi S,Onor M

    更新日期:2008-09-01 00:00:00

  • Responses measured in the exhaled breath of human volunteers acutely exposed to ozone and diesel exhaust.

    abstract::Exhaled breath collection is used to identify and monitor inflammatory or oxidative components in breath. Exhaled breath sample collection is noninvasive and would greatly benefit human pollutant exposure research. We demonstrate the efficacy of exhaled breath collection and analysis in two human exposure studies to o...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Sawyer K,Samet JM,Ghio AJ,Pleil JD,Madden MC

    更新日期:2008-09-01 00:00:00

  • Breath markers for therapeutic radiation.

    abstract::Radiation dose is important in radiotherapy. Too little, and the treatment is not effective, too much causes radiation toxicity. A biochemical measurement of the effect of radiotherapy would be useful in personalisation of this treatment. This study evaluated changes in exhaled breath volatile organic compounds (VOC) ...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Salman D,Eddleston M,Darnley K,Nailon WH,McLaren DB,Hadjithelki A,Ruszkiewicz D,Langejuergen J,Alkhalifa Y,Phillips I,Thomas CLP

    更新日期:2020-10-24 00:00:00

  • Relationship between alveolar nitric oxide concentration in exhaled air and small airway function in COPD.

    abstract::Nitrative stress is thought to be involved in the inflammatory process in COPD airways, and the alveolar nitric oxide concentration (CAlv) has been reported to be increased. However, the CAlv levels are also regulated by gas diffusion at alveolar sites. The aim of the study was to assess the relationship between the C...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Hirano T,Matsunaga K,Sugiura H,Minakata Y,Koarai A,Akamatsu K,Ichikawa T,Furukawa K,Ichinose M

    更新日期:2013-12-01 00:00:00

  • Tablets containing a cysteine protease, actinidine, reduce oral malodor: a crossover study.

    abstract::Tongue coating (TC) mainly consists of protein mostly from exfoliated epithelial cells. Until now, to reduce TC accumulation, only mechanical measures have been available, and the procedure involves unpleasant side effects, such as gagging reflex or carcinogenesis related to mechanical stimulation. We expected that pr...

    journal_title:Journal of breath research

    pub_type: 杂志文章,随机对照试验


    authors: Nohno K,Yamaga T,Kaneko N,Miyazaki H

    更新日期:2012-03-01 00:00:00

  • Breath biomarkers in environmental health science: exploring patterns in the human exposome.

    abstract::The human genome is the counterpart to the human exposome with respect to the gene × environment interaction that describes health state and outcome. The genome has already been sequenced and is in the process of being assessed for specific functionality; to similarly decode the exposome will require the measurement a...

    journal_title:Journal of breath research

    pub_type: 杂志文章,评审


    authors: Pleil JD,Stiegel MA,Sobus JR

    更新日期:2011-12-01 00:00:00

  • Off-line breath acetone analysis in critical illness.

    abstract::Analysis of breath acetone could be useful in the Intensive Care Unit (ICU) setting to monitor evidence of starvation and metabolic stress. The aims of this study were to examine the relationship between acetone concentrations in breath and blood in critical illness, to explore any changes in breath acetone concentrat...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Sturney SC,Storer MK,Shaw GM,Shaw DE,Epton MJ

    更新日期:2013-09-01 00:00:00

  • Bacteria in the airways of patients with cystic fibrosis are genetically capable of producing VOCs in breath.

    abstract::Breath contains hundreds of volatile organic compounds (VOCs), the composition of which is altered in a wide variety of diseases. Bacteria are implicated in the formation of VOCs, but the biochemical mechanisms that lead to the formation of breath VOCs remain largely hypothetical. We hypothesized that bacterial DNA fr...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Bos LD,Meinardi S,Blake D,Whiteson K

    更新日期:2016-12-17 00:00:00

  • Impact of bacterial colonization on exhaled inflammatory markers in wheezing preschool children.

    abstract::Wheeze is a common symptom in preschool children. The role of bacteria, regulatory T (T(reg)) cells and their association with airway inflammation in preschool wheeze is largely unknown. We evaluated inflammatory markers in exhaled breath condensate (EBC), bacterial colonization and circulating T(reg) cells in prescho...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: van de Kant KD,Klaassen EM,van Aerde KJ,Damoiseaux J,Bruggeman CA,Stelma FF,Stobberingh EE,Muris JW,Jöbsis Q,van Schayck OC,Dompeling E

    更新日期:2012-12-01 00:00:00

  • Exhaled breath condensate for lung cancer protein analysis: a review of methods and biomarkers.

    abstract::Lung cancer is a leading cause of cancer-related deaths worldwide, and is considered one of the most aggressive human cancers, with a 5 year overall survival of 10-15%. Early diagnosis of lung cancer is ideal; however, it is still uncertain as to what technique will prove successful in the systematic screening of high...

    journal_title:Journal of breath research

    pub_type: 杂志文章,评审


    authors: Hayes SA,Haefliger S,Harris B,Pavlakis N,Clarke SJ,Molloy MP,Howell VM

    更新日期:2016-07-05 00:00:00

  • Microbial volatile compounds in health and disease conditions.

    abstract::Microbial cultures and/or microbial associated diseases often have a characteristic smell. Volatile organic compounds (VOCs) are produced by all microorganisms as part of their normal metabolism. The types and classes of VOC produced is wide, including fatty acids and their derivatives (e.g. hydrocarbons, aliphatic al...

    journal_title:Journal of breath research

    pub_type: 杂志文章,评审


    authors: Thorn RM,Greenman J

    更新日期:2012-06-01 00:00:00

  • Cancerous glucose metabolism in lung cancer-evidence from exhaled breath analysis.

    abstract::Cancer cells prefer hyperglycolysis versus oxidative phosphorylation, even in the presence of oxygen. This phenomenon is used through the FDG-PET scans, and may affect the exhaled volatile signature. This study investigates the volatile signature in lung cancer (LC) before and after an oral glucose tolerance test (OGT...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Feinberg T,Alkoby-Meshulam L,Herbig J,Cancilla JC,Torrecilla JS,Gai Mor N,Bar J,Ilouze M,Haick H,Peled N

    更新日期:2016-06-07 00:00:00

  • Prediction of blood:air and fat:air partition coefficients of volatile organic compounds for the interpretation of data in breath gas analysis.

    abstract::In this article, a database of blood:air and fat:air partition coefficients (λ b:a and λ f:a) is reported for estimating 1678 volatile organic compounds recently reported to appear in the volatilome of the healthy human. For this purpose, a quantitative structure-property relationship (QSPR) approach was applied and a...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Kramer C,Mochalski P,Unterkofler K,Agapiou A,Ruzsanyi V,Liedl KR

    更新日期:2016-01-27 00:00:00

  • Cluster analysis on breath print of newly diagnosed COPD patients: effects of therapy.

    abstract:BACKGROUND:Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease and airflow limitation and symptoms only partially capture such heterogeneity. Since COPD is known to affect the production of volatile organic compounds (VOCs), we aimed to verify to which extent exhaled VOCs can characterize new...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Scarlata S,Finamore P,Santangelo S,Giannunzio G,Pennazza G,Grasso S,Santonico M,Incalzi RA

    更新日期:2018-06-08 00:00:00

  • Clinical utility of breath ammonia for evaluation of ammonia physiology in healthy and cirrhotic adults.

    abstract::Blood ammonia is routinely used in clinical settings to assess systemic ammonia in hepatic encephalopathy and urea cycle disorders. Despite its drawbacks, blood measurement is often used as a comparator in breath studies because it is a standard clinical test. We sought to evaluate sources of measurement error and pot...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Spacek LA,Mudalel M,Tittel F,Risby TH,Solga SF

    更新日期:2015-12-14 00:00:00

  • Assessment of nanoparticles and metal exposure of airport workers using exhaled breath condensate.

    abstract::Aircraft engine exhaust increases the number concentration of nanoparticles (NP) in the surrounding environment. Health concerns related to NP raise the question of the exposure and health monitoring of airport workers. No biological monitoring study on this profession has been reported to date. The aim was to evaluat...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Marie-Desvergne C,Dubosson M,Touri L,Zimmermann E,Gaude-Môme M,Leclerc L,Durand C,Klerlein M,Molinari N,Vachier I,Chanez P,Mossuz VC

    更新日期:2016-07-13 00:00:00

  • Serum and exhaled breath condensate leptin levels in asthmatic and obesity children: a pilot study.

    abstract:BACKGROUND:Recent studies have highlighted the possible involvement of leptin in inflammation. The leptin receptor is also expressed by alveolar macrophages, T lymphocytes and bronchial epitelial cells, suggesting a possible role in the cascade of airway inflammation. OBJECTIVES:The aim of the study was to evaluate th...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Bodini A,Tenero L,Sandri M,Maffeis C,Piazza M,Zanoni L,Peroni D,Boner A,Piacentini G

    更新日期:2017-09-27 00:00:00

  • Effects of growth and aging on the reference values of pulmonary nitric oxide dynamics in healthy subjects.

    abstract::The lung just like all other organs is affected by age. The lung matures by the age of 20 and age-related changes start around middle age, at 40-50 years. Exhaled nitric oxide (FENO) has been shown to be age, height and gender dependent. We hypothesize that the nitric oxide (NO) parameters alveolar NO (CANO), airway f...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Högman M,Thornadtsson A,Liv P,Hua-Huy T,Dinh-Xuan AT,Tufvesson E,Dressel H,Janson C,Koskela K,Oksa P,Sauni R,Uitti J,Moilanen E,Lehtimäki L

    更新日期:2017-09-13 00:00:00

  • Study of the art: canine olfaction used for cancer detection on the basis of breath odour. Perspectives and limitations.

    abstract::Experimental studies using trained dogs to identify breath odour markers of human cancer, published in the recent decade, have been analyzed and compared with the authors' own results. Particular published studies differ as regards the experimental setup, kind of odour samples (breath, urine, tumor tissue, serum), sam...

    journal_title:Journal of breath research

    pub_type: 杂志文章,评审


    authors: Jezierski T,Walczak M,Ligor T,Rudnicka J,Buszewski B

    更新日期:2015-05-06 00:00:00

  • Influence of age and sex in exhaled breath samples investigated by means of infrared laser absorption spectroscopy.

    abstract::Breath gas analysis provides insight into human metabolism of healthy and ill individuals. As an innovative and non-invasive method, it opens up options to improve diagnostics, monitoring and treatment decisions. Mid-infrared laser absorption spectroscopy is utilized to detect CH(4), H(2)O, CO(2), NH(3) and CH(3)OH in...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Hannemann M,Antufjew A,Borgmann K,Hempel F,Ittermann T,Welzel S,Weltmann KD,Völzke H,Röpcke J

    更新日期:2011-06-01 00:00:00

  • Clinical breath analysis: discriminating between human endogenous compounds and exogenous (environmental) chemical confounders.

    abstract::Volatile organic compounds (VOCs) in exhaled breath originate from current or previous environmental exposures (exogenous compounds) and internal metabolic (anabolic and catabolic) production (endogenous compounds). The origins of certain VOCs in breath presumed to be endogenous have been proposed to be useful as prec...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Pleil JD,Stiegel MA,Risby TH

    更新日期:2013-03-01 00:00:00

  • Smart sensor systems for human health breath monitoring applications.

    abstract::Breath analysis techniques offer a potential revolution in health care diagnostics, especially if these techniques can be brought into standard use in the clinic and at home. The advent of microsensors combined with smart sensor system technology enables a new generation of sensor systems with significantly enhanced c...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Hunter GW,Xu JC,Biaggi-Labiosa AM,Laskowski D,Dutta PK,Mondal SP,Ward BJ,Makel DB,Liu CC,Chang CW,Dweik RA

    更新日期:2011-09-01 00:00:00

  • Quantification of trace levels of the potential cancer biomarkers formaldehyde, acetaldehyde and propanol in breath by SIFT-MS.

    abstract::The sensitivity of selected ion flow tube mass spectrometry, SIFT-MS, has been increased such that it is now possible to detect metabolites present at a part-per-billion, ppb, level in single breath exhalations. However, to utilize this improved sensitivity, the overlaps (coincidences) of those ions resulting from int...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Spaněl P,Smith D

    更新日期:2008-12-01 00:00:00

  • The impact of chewing gum on halitosis parameters: a systematic review.

    abstract::This study aimed to analyze the impact of chewing gum on halitosis parameters. Three databases were searched with the following focused question: 'Can chewing gum additionally reduce halitosis parameters, such as organoleptic scores and volatile sulfur compounds (VSC), when compared to a control treatment'? Controlled...

    journal_title:Journal of breath research

    pub_type: 杂志文章,meta分析,评审


    authors: Muniz FW,Friedrich SA,Silveira CF,Rösing CK

    更新日期:2017-02-17 00:00:00

  • Exhaled breath temperature in patients with stable and exacerbated COPD.

    abstract::The measurement of the peak exhaled breath temperature (EBT) during multiple tidal breaths offers an easy, non-invasive tool for monitoring airway inflammation. Chronic obstructive pulmonary disease (COPD) is linked to airway inflammation, which is further aggravated by exacerbations of the disease. However, the peak ...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Lázár Z,Bikov A,Martinovszky F,Gálffy G,Losonczy G,Horváth I

    更新日期:2014-09-18 00:00:00

  • Methodology validation, intra-subject reproducibility and stability of exhaled volatile organic compounds.

    abstract::Robust methods for breath sampling and analysis are required for potential clinical applications. We have evaluated an improved sampling and experimental design, assessing instrumental and biological variability within and across breath measurements. Calibration curves for selected relevant volatile organic compounds...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Basanta M,Ibrahim B,Douce D,Morris M,Woodcock A,Fowler SJ

    更新日期:2012-06-01 00:00:00

  • Effect of atropine sulfate on gastric emptying and gastrocecal transit time evaluated by using the [1-(13)C]acetic acid and lactose-[(13)C]ureide breath test in conscious rats.

    abstract::Breath tests have been used to investigate many physiological functions. Recently, we have developed a breath test system by using a non-invasive technique in conscious rats. In this study, we investigated the effect of atropine sulfate on the gastric emptying and gastrocecal transit time using [1-(13)C]acetic acid an...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Uchida M,Yoshida K,Shimizu K

    更新日期:2009-12-01 00:00:00

  • Exhaled breath profiling in patients with COPD and OSA overlap syndrome: a pilot study.

    abstract::The analysis of volatile organic compounds (VOCs) by an electronic nose (e-nose) is a groundbreaking method that provides distinct exhaled molecular patterns in several respiratory and systemic diseases. It has been shown that an e-nose can detect obstructive sleep apnea (OSA) as well as chronic obstructive pulmonary ...

    journal_title:Journal of breath research

    pub_type: 杂志文章


    authors: Dragonieri S,Quaranta VN,Carratu P,Ranieri T,Resta O

    更新日期:2016-11-03 00:00:00