Intermittent fasting increases SOD2 and catalase immunoreactivities in the hippocampus but does not protect from neuronal death following transient ischemia in gerbils.

Abstract:

:Intermittent fasting has been shown to have neuroprotective effects against transient focal cerebral ischemic insults. However, the effects of intermittent fasting on transient global ischemic insult has not been studied much yet. The present study examined effects of intermittent fasting on endogenous antioxidant enzyme expression levels in the hippocampus and investigated whether the fasting protects neurons 5 days after 5 min of transient global cerebral ischemia. Gerbils were randomly subjected to either ad libitum or alternate‑day intermittent fasting for two months and assigned to sham surgery or transient ischemia. Changes of antioxidant enzymes were examined using immunohistochemistry for cytoplasmic superoxide dismutase 1 (SOD1), mitochondrial (SOD2), catalase (CAT), and glutathione peroxidase (GPX). The effects of intermittent fasting on ischemia‑induced antioxidant changes, neuronal damage/degeneration and glial activation were examined. The weight of fasting gerbils was not different from that of control gerbils. In controls, SOD1 and GPX immunoreactivities were strong in pyramidal neurons of filed cornu ammonis 1 (CA1). Transient ischemia in controls significantly decreased expressions of SOD1 and GPX in CA1 pyramidal neurons. Intermittent fasting resulted in increased expressions of SOD2 and CAT, not of SOD1 and GPX, in CA1 pyramidal neurons. Nevertheless, CA1 pyramidal neurons were not protected in gerbils subjected to fasting after transient ischemia, and inhibition of glial‑cell activation was not observed in the gerbils. In summary, intermittent fasting for two months increased SOD2 and CAT immunoreactivities in hippocampal CA1 pyramidal neurons. However, fasting did not protect the CA1 pyramidal neurons from transient cerebral ischemia. The results of the present study indicate that intermittent fasting may increase certain antioxidants, but not protect neurons from transient global ischemic insult.

journal_name

Mol Med Rep

authors

Ahn JH,Noh Y,Shin BN,Kim SS,Park JH,Lee TK,Song M,Kim H,Lee JC,Yong JH,Kang IJ,Lee YL,Won MH,Kim JD

doi

10.3892/mmr.2018.9503

subject

Has Abstract

pub_date

2018-12-01 00:00:00

pages

4802-4812

issue

6

eissn

1791-2997

issn

1791-3004

journal_volume

18

pub_type

杂志文章
  • Aloperine inhibits proliferation, migration and invasion and induces apoptosis by blocking the Ras signaling pathway in human breast cancer cells.

    abstract::Aloperine (Alo), as a quinolizidine alkaloid extracted from S. alopecuroide, has the positive activities of anti-inflammatory, anti-allergenic, antitumor and anti-viral. However, the role and mechanism of Alo in breast cancer have not been studied yet. In the present study, Alo markedly inhibited the proliferation and...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9419

    authors: Tian D,Li Y,Li X,Tian Z

    更新日期:2018-10-01 00:00:00

  • Antioxidant effect of angiotensin (1‑7) in the protection of pancreatic β cell function.

    abstract::It is well known that the local renin-angiotensin system (RAS) is activated in the diabetic state, which results in an increase in the level of oxidative stress injury to pancreatic β cells. The angiotensin‑converting enzyme 2 (ACE2)/angiotensin (1‑7) [Ang (1‑7)]/Mas axis is a negative regulator of the classical renin...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5514

    authors: Zhang F,Liu C,Wang L,Cao X,Wang YY,Yang JK

    更新日期:2016-09-01 00:00:00

  • Altered melatonin secretion and circadian gene expression with increased proinflammatory cytokine expression in early-stage sepsis patients.

    abstract::Inflammatory and immune responses, as well as melatonin secretion, are affected by circadian regulation. Abnormal circadian rhythm of melatonin release has been reported to be associated with the later stages of sepsis; however, its role in the early stages of sepsis is unclear. We studied 11 septic and 11 non-septic ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1331

    authors: Li CX,Liang DD,Xie GH,Cheng BL,Chen QX,Wu SJ,Wang JL,Cho W,Fang XM

    更新日期:2013-04-01 00:00:00

  • Bioinformatics analysis on the differentiation of bone mesenchymal stem cells into osteoblasts and adipocytes.

    abstract::The present study aimed to screen several differentially expressed genes (DEGs) and differentially expressed microRNAs (miRNAs) for two types of mesenchymal stem cell (MSC) differentiation. Bone morphogenetic protein 6 (BMP‑6) and dexamethasone were used to induce MSCs towards osteoblastic differentiation or adipocyti...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6178

    authors: Xu X,Jiang H,Li X,Wu P,Liu J,Wang T,Zhou X,Xiong J,Li W

    更新日期:2017-04-01 00:00:00

  • Moscatilin induces apoptosis of pancreatic cancer cells via reactive oxygen species and the JNK/SAPK pathway.

    abstract::Moscatilin is a bibenzyl derivative extracted from the Dendrobium aurantiacum var. denneanum, which has traditionally been used as an immunomodulatory treatment in China. The present study was designed to determine whether moscatilin is a pro‑apoptotic agent in pancreatic cancer, and to elucidate the underlying mechan...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6144

    authors: Zhang L,Fang Y,Xu XF,Jin DY

    更新日期:2017-03-01 00:00:00

  • Effect of silencing TEM8 gene on proliferation, apoptosis, migration and invasion of XWLC‑05 lung cancer cells.

    abstract::Currently, the role of tumor endothelial marker 8 (TEM8) in the occurrence, development, invasion and metastasis of lung cancer and its mechanism are poorly understood. The present study aimed to investigate the effects of TEM8 on proliferation, apoptosis, migration and invasion of XWLC‑05 lung cancer cells. The expre...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7959

    authors: Gong Q,Liu C,Wang C,Zhuang L,Zhang L,Wang X

    更新日期:2018-01-01 00:00:00

  • Development of a dendrimer PAMAM‑based gold biochip for rapid and sensitive detection of endogenous IFN‑γ and anti‑IFN‑γ IgG in patients with hemophagocytic lymphohistiocytosis.

    abstract::Hemophagocytic lymphohistiocytosis (HLH) is a rare but severe disease characterized by immune hyperactivation and cytokine storm. Given the high mortality rate of HLH, there is a need for more effective diagnostic tools and treatments. The present study developed a dendrimer‑based protein biochip for rapid, sensitive ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11605

    authors: Du YX,Ye L,Song ZJ,Lv H,Liu Q,Li SG,Liu SS,Hong J,Gao Y,Schneider ME,Du WD

    更新日期:2020-12-01 00:00:00

  • Endothelial microparticles activate endothelial cells to facilitate the inflammatory response.

    abstract::Endothelial microparticles (EMPs) and endothelial cells (ECs) are involved in the pathophysiological mechanisms of sepsis and septic shock. EMPs are small vesicles released by ECs and are considered biomarkers for endothelial cell function and mediators for intercellular information exchange. However, the effect of EM...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6113

    authors: Liu Y,Zhang R,Qu H,Wu J,Li L,Tang Y

    更新日期:2017-03-01 00:00:00

  • Long intergenic non‑coding RNA‑p21 mediates cardiac senescence via the Wnt/β‑catenin signaling pathway in doxorubicin-induced cardiotoxicity.

    abstract::Doxorubicin (Dox)-induced cardiotoxicity has been a well‑known phenomenon to clinicians and scientists for decades. It has been confirmed that Dox‑dependent cardiotoxicity is accompanied by cardiac cellular senescence. However, the molecular mechanisms underlying Dox cardiotoxicity remains to be fully elucidated. Long...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8169

    authors: Xie Z,Xia W,Hou M

    更新日期:2018-02-01 00:00:00

  • Differential expression of microRNAs and their target genes in non-small-cell lung cancer.

    abstract::MicroRNAs (miRNAs) are single‑stranded RNA species that constitute a class of non‑coding RNAs, and are emerging as key regulators of gene expression. Since each miRNA is capable of regulating multiple genes, miRNAs are attractive markers for studies of coordinated gene expression. In this study, we investigated miRNA ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2890

    authors: Lee HY,Han SS,Rhee H,Park JH,Lee JS,Oh YM,Choi SS,Shin SH,Kim WJ

    更新日期:2015-03-01 00:00:00

  • Identification of androgen-regulated genes in human prostate.

    abstract::Androgens are essential for the development of the prostate and prostate cancer. We examined androgen-regulated gene expression in the human prostate. Samples from benign and malignant prostate tissue and samples containing prostate tissue obtained from prostate cancer patients thre...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.956

    authors: Vaarala MH,Hirvikoski P,Kauppila S,Paavonen TK

    更新日期:2012-09-01 00:00:00

  • Expression profile of plasma microRNAs in premature infants with respiratory distress syndrome.

    abstract::As well-known regulators of gene expression, microRNAs (miRNAs) are important not only in cell proliferation and differentiation, but also in tumorigenesis and organ development. It has been estimated that miRNAs may be responsible for regulating the expression of almost one third of the human genome. Simultaneously, ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3699

    authors: Kan Q,Ding S,Yang Y,Zhou X

    更新日期:2015-08-01 00:00:00

  • Gene expression of adipose tissue, endothelial cells and platelets in subjects with metabolic syndrome (Review).

    abstract::Metabolic syndrome is a combination of medical disorders including hypertension, dyslipidemia, hyperglycemia, insulin resistance and increased waist circumference, and is associated with a higher risk of cardiovascular disease. An increase in adipose tissue mass is associated with t...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,评审

    doi:10.3892/mmr.2012.785

    authors: Pérez PM,Moore-Carrasco R,González DR,Fuentes EQ,Palomo IG

    更新日期:2012-05-01 00:00:00

  • Comprehensive analysis of a microRNA expression profile in pediatric medulloblastoma.

    abstract::Medulloblastoma is the most common malignant brain tumor of the central nervous system among children. Medulloblastoma is an embryonal tumor, of which little is known about the pathogenesis. Several efforts have been made to understand the molecular aspects of its tumorigenic pathways; however, these are poorly unders...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6490

    authors: Dai J,Li Q,Bing Z,Zhang Y,Niu L,Yin H,Yuan G,Pan Y

    更新日期:2017-06-01 00:00:00

  • Characterization of pancreatic ductal adenocarcinoma using whole transcriptome sequencing and copy number analysis by single-nucleotide polymorphism array.

    abstract::The aim of the current study was to implement whole transcriptome massively parallel sequencing (RNASeq) and copy number analysis to investigate the molecular biology of pancreatic ductal adenocarcinoma (PDAC). Samples from 16 patients with PDAC were collected by ultrasound‑guided biopsy or from surgical specimens for...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4344

    authors: Di Marco M,Astolfi A,Grassi E,Vecchiarelli S,Macchini M,Indio V,Casadei R,Ricci C,D'Ambra M,Taffurelli G,Serra C,Ercolani G,Santini D,D'Errico A,Pinna AD,Minni F,Durante S,Martella LR,Biasco G

    更新日期:2015-11-01 00:00:00

  • Promoter methylation profile of GSTP1 and RASSF1A in benign hyperplasia and metastatic prostate cancer patients in a Kashmiri population.

    abstract::Promoter hypermethylation is a marginal approach to inactivating tumor suppressor genes in cancer. DNA hypermethylation is a well-recognized epigenetic malfunction observed in several malignancies, most predominantly in prostate cancer. Aberrant DNA methylation patterns are considered to be the earliest somatic genome...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2010.348

    authors: Syeed N,Syed Sameer A,Hamid A,Shah ZA,Afroze D,Rasool R,Siddiqi MA

    更新日期:2010-09-01 00:00:00

  • Diosmetin inhibits the metastasis of hepatocellular carcinoma cells by downregulating the expression levels of MMP-2 and MMP-9.

    abstract::Hepatocellular carcinoma (HCC) is one of the most malignant types of tumor worldwide with a high rate of mortality. Diosmetin (DIOS) exhibits various activities, including anticancer activities. However, the role of DIOS in the metastasis of HCC, and its underlying molecular mechanism, remain to be fully elucidated. I...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.4872

    authors: Liu J,Wen X,Liu B,Zhang Q,Zhang J,Miao H,Zhu R

    更新日期:2016-03-01 00:00:00

  • RBEL1 is required for osteosarcoma cell proliferation via inhibiting retinoblastoma 1.

    abstract::Osteosarcoma is the most common type of primary malignant tumor of the bone. However, mechanisms underlying osteosarcoma cell proliferation are poorly understood. The present study shows that RBEL1, a newly identified Rab-like GTPase, may be a key regulator of osteosarcoma cell proliferation. Knockdown of RBEL1 in ost...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4670

    authors: Tang H,Ji F,Sun J,Xie Y,Xu Y,Yue H

    更新日期:2016-02-01 00:00:00

  • Lentivirally overexpressed T-bet regulates T-helper cell lineage commitment in chronic hepatitis B patients.

    abstract::Chronic hepatitis B virus (HBV) infection is commonly considered to occur as a result of disturbance of the immune system. T-box expressed in T cells (T-bet) is an essential transcription factor for T helper (Th) cell differentiation and function. The aim of this study was to investigate the effect of T-bet overexpres...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.905

    authors: Liu X,Tang Z,Zhang Y,Hu J,Li D,Zang G,Yu Y

    更新日期:2012-08-01 00:00:00

  • CD-PLLD co-delivering docetaxel and MMP-9 siRNA plasmid for nasopharyngeal carcinoma therapy in vivo.

    abstract::The co-delivery of a drug and a target gene has become a primary strategy in cancer therapy. Based on our previous study, a synthesized star‑shaped co‑polymer consisting of β‑cyclodextrin (CD) and a poly(L‑lysine) dendron (PLLD) was used to co-deliver docetaxel (DOC) and matrix metalloproteinase 9 (MMP‑9) small interf...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6715

    authors: Liu T,Wu X,Wang Y,Hou X,Jiang G,Wu T,Xie H,Xie M

    更新日期:2017-08-01 00:00:00

  • Matrine suppresses cardiac fibrosis by inhibiting the TGF‑β/Smad pathway in experimental diabetic cardiomyopathy.

    abstract::Cardiac fibrosis is one of the pathological characteristics of diabetic cardiomyopathy (DbCM). Matrine treatment has proven to be effective in cases of organ fibrosis and cardiovascular diseases. In the present study, the anti-fibrosis-associated cardioprotective effects of matrine on DbCM were investigated. Rats with...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8054

    authors: Zhang Y,Cui L,Guan G,Wang J,Qiu C,Yang T,Guo Y,Liu Z

    更新日期:2018-01-01 00:00:00

  • TRESK gene recombinant adenovirus vector inhibits capsaicin-mediated substance P release from cultured rat dorsal root ganglion neurons.

    abstract::The present study was conducted to determine whether the activation of TRESK in the dorsal root ganglion (DRG) by the TRESK gene recombinant adenovirus vector inhibits the capsaicin-evoked substance P (SP) release using a radioimmunoassay. TRESK is an outwardly rectifying K+ current...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.778

    authors: Zhou J,Yao SL,Yang CX,Zhong JY,Wang HB,Zhang Y

    更新日期:2012-04-01 00:00:00

  • Liriopesides B from Liriope spicata var. prolifera inhibits metastasis and induces apoptosis in A2780 human ovarian cancer cells.

    abstract::Ovarian cancer is the most frequent cause of death among gynecological cancers. In the present study, the anti‑cancer effect of liriopesides B, a steroidal saponin from Liriope spicata var. prolifera, against A2780 cells was investigated. Transwell chambers were adopted to assess its effect on cell invasion and chemot...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11256

    authors: Yu H,Wang H,Yin Y,Wang Z

    更新日期:2020-09-01 00:00:00

  • Synergistic neuroprotective effect of microglial‑conditioned media treated with geniposide and ginsenoside Rg1 on hypoxia injured neurons.

    abstract::The synergistic mechanism underlying the effects of multi‑component combined drug use for complex diseases remains to be fully elucidated. Microglial activation following ischemia can either affect neural survival or cause neuronal injury. The aim of the present study was to determine the synergistic effect of genipos...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4094

    authors: Wang J,Hou J,Lei H,Fu J,Pan Y,Liu J

    更新日期:2015-10-01 00:00:00

  • Altered expression of genes involved in brain energy metabolism as adaptive responses in rats exposed to chronic variable stress; changes in cortical level of glucogenic and neuroactive amino acids.

    abstract::Brain metabolism is closely associated with neuronal activity and enables the accurate synthesis and function of neurotransmitters. Although previous studies have demonstrated that chronic stress is associated with the overproduction of reactive oxygen species (ROS), which leads to oxidative stress and the disruption ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9865

    authors: Herbet M,Natorska-Chomicka D,Korga A,Ostrowska M,Izdebska M,Gawrońska-Grzywacz M,Piątkowska-Chmiel I,Pawłowski K,Ślaska B,Poleszak E,Dudka J

    更新日期:2019-03-01 00:00:00

  • MicroRNA‑296 targets AKT2 in pancreatic cancer and functions as a potential tumor suppressor.

    abstract::Although microRNA-296 (miR-296) has been studied in various types of human cancer, its expression, biological role and mechanism of action in pancreatic cancer remains to be elucidated. The aim of the current study was to investigate the expression level, possible roles and underlying molecular mechanisms of miR‑296 i...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6602

    authors: Li H,Li J,Shi B,Chen F

    更新日期:2017-07-01 00:00:00

  • Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction.

    abstract::Physical exercise is beneficial for the functional recovery of neurons after stroke. It has been suggested that exercise regulates proliferation and differentiation of endogenous neural stem cells (NSCs); however, the underlying molecular mechanisms are still largely unknown. In the present study, the aim was to inves...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9147

    authors: Liu W,Wu W,Lin G,Cheng J,Zeng Y,Shi Y

    更新日期:2018-08-01 00:00:00

  • Curcumin inhibits advanced glycation end product-induced oxidative stress and inflammatory responses in endothelial cell damage via trapping methylglyoxal.

    abstract::Methylglyoxal (MGO)-induced carbonyl stress and pro-inflammatory responses have been suggested to contribute to endothelial dysfunction. Curcumin (Cur), a polyphenolic compound from Curcuma longa L., may protect endothelial cells against carbonyl stress-induced damage by trapping dicarbonyl compounds such as MGO. Howe...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4725

    authors: Sun YP,Gu JF,Tan XB,Wang CF,Jia XB,Feng L,Liu JP

    更新日期:2016-02-01 00:00:00

  • Gangliosides and CD82 inhibit the motility of colon cancer by downregulating the phosphorylation of EGFR at different tyrosine sites and signaling pathways.

    abstract::Previous studies have shown that (GM3), a ganglioside, suppresses hepatoma cell motility and migration by inhibiting phosphorylation of EGFR and the activity of the PI3K/AKT signaling pathway. Therefore, the aim of the present study was to investigate whether the combined treatment of CD82 with gangliosides can exert ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11467

    authors: Huang X,Li Y,He X,Chen Y,Wei W,Yang X,Ma K

    更新日期:2020-11-01 00:00:00

  • Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis.

    abstract::Interleukin (IL)-1β-induced chondrocyte apoptosis is associated with the pathogenesis of arthritis. Platelet‑rich plasma (PRP), which is derived from the patient's own blood and contains numerous growth factors, has the potential for arthritis treatment. Therefore, the present study aimed to determine the effects of P...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5767

    authors: Yang J,Lu Y,Guo A

    更新日期:2016-11-01 00:00:00