Identification of trophoblast-specific elements in the HLA-C core promoter.

Abstract:

:There are several aspects of HLA-C gene expression that distinguish it from the HLA-A and HLA-B genes. First, HLA-C is expressed by extravillous trophoblasts, whereas HLA-A and HLA-B are not. Second, its cell-surface expression is much lower, which has been linked to changes in transcription and efficiency of peptide loading and export. Third, HLA-C possesses a NK cell-specific promoter and a complex alternative splicing system that regulates expression during NK cell development. In this study, we investigate the contribution of the HLA-C core promoter to trophoblast-specific expression. Analysis of transcription start sites showed the presence of a trophoblast-associated start site and additional upstream TATA and CCAAT-box elements in the HLA-C promoter, suggesting the presence of an overlapping trophoblast-specific promoter. A comparison of in vitro promoter activity showed that the HLA-C promoter was more active in trophoblast cell lines than either the HLA-A or HLA-B promoters. Enhanced trophoblast activity was mapped to the central enhanceosome region of the promoter, and mutational analysis identified changes in the RFX-binding region that generated a trophoblast-specific enhancer.

journal_name

HLA

journal_title

HLA

authors

Johnson JK,Wright PW,Li H,Anderson SK

doi

10.1111/tan.13404

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

288-297

issue

5

eissn

2059-2302

issn

2059-2310

journal_volume

92

pub_type

杂志文章

相关文献

HLA文献大全