Abstract:
:By rosetting with SRBC coupled to rabbit-anti-human IgM, the surface IgM-negative cells of human fetal bone marrow were enriched, and subsequently infected and transformed by Epstein-Barr virus (EBV). Single clones of the transformed cells were obtained. Ninety percent of the resulting cell clones were surface-immunoglobulin-negative, and of 8 clones which were further studied, 5 lacked intracellular, cytoplasmic Ig as measured by immunofluorescence. Control cell clones derived from the same material without pre-selection expressed surface Ig and also secreted Ig. Utilization of a panel of B-cell-specific monoclonal antibodies (MAbs) showed no difference between the cell clones expressing surface Ig and those that did not. The progenitor B-cell lines did not show a phenotype resembling that of cell lines derived from B-cell malignancies, such as high agarose clonability. In spite of their immature Ig-phenotype, these clones showed rearrangement of at least one heavy chain Ig-allele. Efforts to induce differentiation in these clones were unsuccessful. These clones may represent progenitor B cells, or B cells with faulty heavy-chain rearrangement. EBV can apparently be used as a tool to derive cell lines representing different levels of B-cell differentiation, and can also transform immature B cells, which may be useful in the analysis of B-cell differentiation.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Ernberg I,Falk K,Hansson Mdoi
10.1002/ijc.2910390212subject
Has Abstractpub_date
1987-02-15 00:00:00pages
190-7issue
2eissn
0020-7136issn
1097-0215journal_volume
39pub_type
杂志文章abstract::ERK and RSK2 drive proliferation and invasion of many cancers. Phosphoprotein enriched in astrocytes 15 (PEA15) binds ERK and RSK2 and high PEA15 levels can impair ERK- and RSK2-dependent transcription. PEA15 expression also inversely correlates with cell motility and invasiveness. We therefore tested PEA15 effects on...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27415
更新日期:2012-10-01 00:00:00
abstract::The majority of lung cancer patients have tumor-derived genetic alterations in circulating plasma DNA that could be exploited as a diagnostic tool. We used fluorescent microsatellite analysis to detect alterations in plasma and tumor DNA in 34 patients who underwent bronchoscopy for lung cancer, including 11 small cel...
journal_title:International journal of cancer
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abstract::Although early detection and systemic therapies have improved the diagnosis and clinical cure rate of breast cancer, breast cancer remains the most frequently occurring malignant cancer in women due to a lack of sufficiently effective treatments. Thus, to develop potential targeted therapies and thus benefit more pati...
journal_title:International journal of cancer
pub_type: 杂志文章
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更新日期:2020-07-15 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29011
更新日期:2015-02-01 00:00:00
abstract::Several pathways have been implicated in the pathogenesis of endometrial carcinoma. Based on recent reports, BRAF mutations provide an alternative route for activation of the RAS signalling pathway. The CDKN2A (p16) tumour suppressor gene is also altered in several tumour types. We therefore wanted to assess the patte...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20702
更新日期:2005-07-20 00:00:00
abstract::Leukocyte migration inhibition (LMI) assays were performed using 3 M KCI extracts to detect cell-mediated immune reactions against tumor-associated antigens (TAA) of swine melanoma. Positive LMI reactivity was 70% or greater in melanoma-bearing swine compared to 10% reactivity or less in normal swine tested with extra...
journal_title:International journal of cancer
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journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960503)66:3<315::AID-IJC
更新日期:1996-05-03 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10103
更新日期:2002-02-20 00:00:00
abstract::Endothelial lipase (LIPG) is a cell surface associated lipase that displays phospholipase A1 activity towards phosphatidylcholine present in high-density lipoproteins (HDL). LIPG was recently reported to be expressed in breast cancer and to support proliferation, tumourigenicity and metastasis. Here we show that sever...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32138
更新日期:2019-08-15 00:00:00
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journal_title:International journal of cancer
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doi:10.1002/ijc.10413
更新日期:2002-06-20 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25474
更新日期:2011-04-01 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29899
更新日期:2016-04-01 00:00:00
abstract::The Hong Kong Chinese population has an unusually high incidence of colorectal cancer in the young, suggestive of hereditary susceptibility. To search for a genetic basis for this predisposition, we studied the incidence of microsatellite instability (MSI) in paraffin-embedded colectomy specimens of 124 young (<50 yea...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20000720)89:4<356::aid-ijc7>3.0.
更新日期:2000-07-20 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1002/ijc.30127
更新日期:2016-08-15 00:00:00
abstract::There is limited research on the effect of dietary quality on hepatocellular carcinoma (HCC) risk in populations with relatively high risk of HCC. Using data from Singapore Chinese Health Study, a prospective cohort study, of 63 257 Chinese aged 45 to 74, we assessed four diet-quality index (DQI) scores: the Alternati...
journal_title:International journal of cancer
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journal_title:International journal of cancer
pub_type: 杂志文章
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更新日期:1997-11-04 00:00:00
abstract::The present study was undertaken to define the gene(s) of importance on the long arm of chromosome 18 (chromosome 18q) in endometrial carcinomas. We analyzed loss of heterozygosity (LOH) at 3 loci on chromosome 18q and DCC gene expression by the reverse-transcriptase/polymerase chain reaction (RT-PCR) method. Among 61...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570407
更新日期:1994-05-15 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10387
更新日期:2002-05-20 00:00:00
abstract::Mouse-transformed keratinocytes cultured in the presence of transforming growth factor beta1 (TGF-beta1) acquire an array of morphologic and functional properties that give rise to a migratory phenotype that expresses mesenchymal molecular markers. This cellular conversion involves activation of the Ras-ERK pathway, e...
journal_title:International journal of cancer
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更新日期:2003-12-10 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章,meta分析,评审
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更新日期:2014-05-15 00:00:00
abstract::The relative expression of estrogen receptor (ER) and progesterone receptor (PR) mRNA transcripts was measured in 71 primary breast-cancer biopsies. ER and PR binding activity were estimated in parallel by the dextran-coated-charcoal method. There was a close correlation between the amount of ER mRNA and estradiol bin...
journal_title:International journal of cancer
pub_type: 杂志文章
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journal_title:International journal of cancer
pub_type: 杂志文章
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更新日期:1997-09-17 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章
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更新日期:2015-04-15 00:00:00
abstract::9-cis-Retinoic acid (9cRA) and 1alpha,25-dihydroxyvitamin D3 (1,25D) show promise as potential chemopreventive agents. We examined 9cRA and 1,25D, alone and in combination, for their potential to inhibit carcinogen (NNK)-induced lung carcinogenesis in A/J mice. A/J mice (n=14/group) were treated with 9cRA (7.5, 15, or...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22462
更新日期:2007-04-01 00:00:00
abstract::Breast cancer (BC) and diabetes mellitus (DM) are major health problems. We examined the association between DM and BC stage at diagnosis and subsequent survival in a Finnish cohort of female BC patients. All BC cases (N = 73,170) diagnosed in 1995-2013 with dates and causes of death were identified from the Finnish C...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31250
更新日期:2018-06-01 00:00:00
abstract::Azathioprine (AZ) was injected into C57 Black mice before or after IM implantation of the syngeneic fibrosarcoma FS6, and observations were made on tumour growth and on the cellular composition of the fibrosarcomas. When multiple injections were given to the tumour-bearing host the tumours regressed. The percentage of...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910200119
更新日期:1977-07-15 00:00:00
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journal_title:International journal of cancer
pub_type: 杂志文章
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更新日期:1997-05-29 00:00:00
abstract::A long-term CD4-CD8- TCR alpha beta human T-cell line, as well as similar CD4-CD8- TCR gamma delta T-cell lines for comparison, were generated from various tissues by negative selection using anti-CD4 and anti-CD8 monoclonal antibodies (MAbs) followed by positive selection with specific anti-TCR MAb and then repeated ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:1991-04-22 00:00:00
abstract::The presence of TGF alpha mRNA has been reported previously to occur in primary colon cancers. We report the expression of the normal 4.5 kb TGF alpha transcript in the mucosa of the normal human gastro-intestinal tract from oesophagus through to colon. The highest levels of human TGF alpha mRNA occurred in the duoden...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430305
更新日期:1989-03-15 00:00:00
abstract::Osteosarcoma incidence rates in the United States peak in adolescence and in the elderly. The international patterns of osteosarcoma incidence in children have been described, whereas those for young, middle age or elderly adults have not. Using the Cancer Incidence in Five Continents, International Agency for Cancer ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24320
更新日期:2009-07-01 00:00:00