Abstract:
:DNA sequence analysis and electrophoresis in denaturing gel revealed that a 60 base pair insertion which had been previously postulated on the basis of native polyacrylamide gel electrophoresis of mitochondrial DNA from Japanese (Horai and Matsunaga 1986) did not exist at all. Unusual behavior of certain restriction fragments in native polyacrylamide gels apparently resulted in what appeared to be an insertion. Further study revealed that this behavior is most likely due to secondary structures of the fragments. The results of the present study suggest that adequate care should be taken when assessing molecular weights of restriction fragments by native polyacrylamide gel electrophoresis.
journal_name
Hum Genetjournal_title
Human geneticsauthors
Horai S,Inoue T,Matsunaga Edoi
10.1007/BF00273844subject
Has Abstractpub_date
1987-01-01 00:00:00pages
73-4issue
1eissn
0340-6717issn
1432-1203journal_volume
75pub_type
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