An apparent discrepancy between chain length and electrophoretic mobility of restriction fragments: a case of human mitochondrial DNA.

Abstract:

:DNA sequence analysis and electrophoresis in denaturing gel revealed that a 60 base pair insertion which had been previously postulated on the basis of native polyacrylamide gel electrophoresis of mitochondrial DNA from Japanese (Horai and Matsunaga 1986) did not exist at all. Unusual behavior of certain restriction fragments in native polyacrylamide gels apparently resulted in what appeared to be an insertion. Further study revealed that this behavior is most likely due to secondary structures of the fragments. The results of the present study suggest that adequate care should be taken when assessing molecular weights of restriction fragments by native polyacrylamide gel electrophoresis.

journal_name

Hum Genet

journal_title

Human genetics

authors

Horai S,Inoue T,Matsunaga E

doi

10.1007/BF00273844

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

73-4

issue

1

eissn

0340-6717

issn

1432-1203

journal_volume

75

pub_type

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