Induction kinetics of mutations at two genetic loci, DNA damage and repair in CHO cells after different exposure times to N-ethyl-N-nitrosourea.


:Ouabain resistance (ouar) and 6-thioguanine resistance (6-TGr) mutation frequencies were measured in Chinese hamster ovary cells after treatment with N-ethyl-N-nitrosourea (ENU) for varying periods of time. Maximal mutation frequency at the Na+/K+ ATPase gene locus (ouar mutations) was attained within 5 min of exposure, whereas the mutation frequency at the hypoxanthine guanine phosphoribosyltransferase locus (6-TGr mutations) continued to increase up to 60 min, following the theoretical curve for exponential decay of ENU with time. Detection of DNA single strand breaks (ssb) by alkaline elution showed that maximal levels were attained within 5 min of treatment with ENU. Fast repair of DNA ssb occurred early after exposure (greater than 50% repair within 10 min). Analysis of DNA ethylation products by h.p.l.c. showed initially rapid removal of O2-ethylcytosine (25% in the first hour), slow removal of 7-ethylguanine, 3-ethyladenine and 3-ethylguanine and no removal at all of O6-ethylguanine, O4-ethylthymine and ethylphosphotriesters. These time-course studies reveal different target gene responses in the fixation of DNA damage into mutations.






Dogliotti E,Vitelli A,Terlizzese M,Di Muccio A,Calcagnile A,Saffiotti U,Bignami M




Has Abstract


1987-01-01 00:00:00












  • The role of the Peyer's patch in carcinogenesis. II. 3-Methylcholanthrene-induced lymphoid malignancies in rat Peyer's patches.

    abstract::To determine whether B or T lymphoid malignancies could be induced following the exposure of lymphoid cells within different lymphoid organs to a potential chemical carcinogen, 3-methylcholanthrene (3-MC) was directly injected into surgically exposed Peyer's patches (PP), mesenteric lymph nodes (MLN), axillary lymph n...


    pub_type: 杂志文章


    authors: Bost KL,Garrett LR,Cuchens MA

    更新日期:1986-08-01 00:00:00

  • Urinary levels of volatile organic carcinogen and toxicant biomarkers in relation to lung cancer development in smokers.

    abstract::Besides polycyclic aromatic hydrocarbons (PAH) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), which are established lung carcinogens, tobacco smoke also contains relatively large quantities of volatile organic carcinogens and toxicants, including 1,3-butadiene, ethylene oxide, benzene, acrolein and crotonal...


    pub_type: 杂志文章


    authors: Yuan JM,Gao YT,Wang R,Chen M,Carmella SG,Hecht SS

    更新日期:2012-04-01 00:00:00

  • Kinetics of DNA adduct formation and removal in mouse hepatocytes following in vivo exposure to 5,9-dimethyldibenzo[c,g]carbazole.

    abstract::5,9-Dimethyldibenzo[c,g]carbazole (DMDBC), a potent mouse hepatocarcinogen, has been shown to induce a non-linear increase in mutant frequency in the liver of the transgenic MutaMouse. To gain insight into the mechanisms underlying the mutagenicity of DMDBC in vivo, DNA damage formation and removal were monitored in m...


    pub_type: 杂志文章


    authors: Renault D,Brault D,Lossouarn Y,Périn-Roussel O,Taras-Valéro D,Périn F,Thybaud V

    更新日期:2000-02-01 00:00:00

  • Effects of catechol on the induction of tumors in mouse skin by 7,8-dihydroxy-7,8-dihydrobenzo[a]pyrenes.

    abstract::Catechol (1,2-dihydroxybenzene) is a major phenolic compound present in the co-carcinogenic fraction of cigarette tar. It has been shown to be a potent co-carcinogen with benzo[a]pyrene (BaP) in mouse skin. In this study we have examined the co-carcinogenic and co-initiating activities of catechol with racemic and ena...


    pub_type: 杂志文章


    authors: Melikian AA,Jordan KG,Braley J,Rigotty J,Meschter CL,Hecht SS,Hoffmann D

    更新日期:1989-10-01 00:00:00

  • P53 point mutations in initial superficial bladder cancer occur only in tumors from current or recent cigarette smokers.

    abstract::Sequencing of p53 exons 5-8 was carried out on 51 initial superficial bladder tumors selected on the basis of high grade and/or p53 overexpression (immunohistochemistry without antigen retrieval). Fourteen point mutations in 13 tumors and one 21 bp deletion in another tumor were identified. In addition, a germ-line mu...


    pub_type: 杂志文章


    authors: LaRue H,Allard P,Simoneau M,Normand C,Pfister C,Moore L,Meyer F,Têtu B,Fradet Y

    更新日期:2000-01-01 00:00:00

  • Stimulation of DNA synthesis and c-fos mRNA expression in primary rat hepatocytes by estrogens.

    abstract::The mechanism(s) of tumour promotion in liver by estrogens is not well understood although growth stimulation is known to be one important element of their action. As a basis for studying mechanisms of growth control by estrogens, effects of both natural and synthetic estrogens on DNA synthesis and protooncogene c-fos...


    pub_type: 杂志文章


    authors: Lee CH,Edwards AM

    更新日期:2001-09-01 00:00:00

  • Inhibition of neoplastic development in rat liver, kidney, oesophagus and forestomach by 4,4'-diaminodiphenylmethane administration.

    abstract::The modifying effects of 4,4'-diaminodiphenylmethane (DDPM) (0.1% in diet) administration on liver carcinogenesis induced by 2-acetylaminofluorene (2-AAF) (0.02% in diet), 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) ( 0.06% in diet), diethylnitrosamine (DEN) (0.001% in drinking water) and N-ethyl-N-hydroxyethylnit...


    pub_type: 杂志文章


    authors: Tsuda H,Ogiso T,Hasegawa R,Imaida K,Masui T,Ito N

    更新日期:1987-05-01 00:00:00

  • TIPE1 promotes cervical cancer progression by repression of p53 acetylation and is associated with poor cervical cancer outcome.

    abstract::Previous studies have shown that TIPE1 inhibits tumor proliferation and metastasis in certain cancers; however, increased expression of TIPE1 is observed in cervical cancer cell lines and tissues, indicating it might exert a distinctive role in cervical cancer. Cell and xenograft tumorigenicity assays showed that TIPE...


    pub_type: 杂志文章


    authors: Zhao P,Pang X,Jiang J,Wang L,Zhu X,Yin Y,Zhai Q,Xiang X,Feng F,Xu W

    更新日期:2019-06-10 00:00:00

  • A COX-2 inhibitor prevents the esophageal inflammation-metaplasia-adenocarcinoma sequence in rats.

    abstract::Barrett's esophagus (BE) and esophageal adenocarcinoma (ADC) is associated with reflux of duodenal contents. Cyclooxygenase (COX)-2 is over-expressed in BE and ADC, and supposedly contributes to esophageal carcinogenesis. The aim of this study is to investigate what effect a COX-2 inhibitor has on esophageal adenocarc...


    pub_type: 杂志文章


    authors: Oyama K,Fujimura T,Ninomiya I,Miyashita T,Kinami S,Fushida S,Ohta T,Koichi M

    更新日期:2005-03-01 00:00:00

  • Detection of acrolein and crotonaldehyde DNA adducts in cultured human cells and canine peripheral blood lymphocytes by 32P-postlabeling and nucleotide chromatography.

    abstract::People are constantly being exposed to toxic and carcinogenic aldehydes. However, little is actually known about the mechanisms underlying the toxic and carcinogenic effects of these aldehydes on human cells. The DNA alkylating activities of two of the more toxic and environmentally prominent alpha,beta-unsaturated al...


    pub_type: 杂志文章


    authors: Wilson VL,Foiles PG,Chung FL,Povey AC,Frank AA,Harris CC

    更新日期:1991-08-01 00:00:00

  • Inhibitory effect of curcumin on 12-O-tetradecanoylphorbol-13-acetate-induced increase in ornithine decarboxylase mRNA in mouse epidermis.

    abstract::Application of 5 nmol 12-O-tetradecanoylphorbol-13-acetate (TPA) to the skin of female CD-1 mice led to a rapid increase in the concentration of epidermal ornithine decarboxylase (ODC) mRNA from an undetectable level in control mice to a high maximum level at 4-5 h after TPA administration. The concentration of epider...


    pub_type: 杂志文章


    authors: Lu YP,Chang RL,Huang MT,Conney AH

    更新日期:1993-02-01 00:00:00

  • Smad-Sp1 complexes mediate TGFbeta-induced early transcription of oncogenic Smad7 in pancreatic cancer cells.

    abstract::The transcription factor Sp1 has been implicated in cell-type-specific activation of transforming growth factor-beta (TGFbeta) target genes in normal epithelial cells as well as in aberrant gene activation by TGFbeta in epithelial tumor cells. Here, we have examined the interaction of Sp1 with components of the Smad s...


    pub_type: 杂志文章


    authors: Jungert K,Buck A,Buchholz M,Wagner M,Adler G,Gress TM,Ellenrieder V

    更新日期:2006-12-01 00:00:00

  • RSK4 inhibition results in bypass of stress-induced and oncogene-induced senescence.

    abstract::p90 Ribosomal S6 kinase (RSK) 4 is a serine-threonine kinase that belongs to the p90RSK family. RSK4 has been proposed as a tumor suppressor gene, related with anti-invasive activity, inhibition of the RAS-mitogen-activated protein kinase (MAPK) pathway and induction of senescence. Despite the related findings, little...


    pub_type: 杂志文章


    authors: López-Vicente L,Pons B,Coch L,Teixidó C,Hernández-Losa J,Armengol G,Ramon Y Cajal S

    更新日期:2011-04-01 00:00:00

  • Metabolism of benzo[a]pyrene in human epidermal keratinocytes in culture.

    abstract::The metabolism of benzo[a]pyrene (BP) in cultured human epidermal keratinocytes was investigated using thin layer chromatography, high pressure liquid chromatography and cell-mediated mutagenesis assay. Epidermal keratinocytes were obtained from skin of normal subjects and all experiments were performed on primary cul...


    pub_type: 杂志文章


    authors: Kuroki T,Nemoto N,Kitano Y

    更新日期:1980-07-01 00:00:00

  • Identification and quantitation of DNA adducts from calf thymus DNA exposed to 3,4-epoxy-1-butene.

    abstract::3,4-Epoxy-1-butene (EB) is the major mutagenic metabolite of butadiene (BD), an important industrial chemical classified as a probable human carcinogen. Although the mechanism of carcinogenicity of EB is not known, its reactions with nucleophilic sites of DNA giving pro-mutagenic lesions are likely to constitute the e...


    pub_type: 杂志文章


    authors: Tretyakova N,Lin Y,Sangaiah R,Upton PB,Swenberg JA

    更新日期:1997-01-01 00:00:00

  • G to A transitions and G to T transversions in codon 12 of the Ki-ras oncogene isolated from mouse lung tumors induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and related DNA methylating and pyridyloxobutylating agents.

    abstract::Lung tumors were induced in A/J mice by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and the related compounds acetoxymethylmethylnitrosamine (AMMN) and 4-acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone (NNKOAc). NNK both methylates and pyridyloxobutylates DNA while AMMN and...


    pub_type: 杂志文章


    authors: Ronai ZA,Gradia S,Peterson LA,Hecht SS

    更新日期:1993-11-01 00:00:00

  • Genetic variants in caspase genes and susceptibility to non-Hodgkin lymphoma.

    abstract::The caspase proteins are essential for the regulation of normal B cell development and regulation of apoptosis. We investigated five single nucleotide polymorphisms in four key caspase genes, CASP3 [Ex8-280C>A (rs6948) and Ex8+567T>C (rs1049216)], CASP8 Ex14-271A>T (rs13113), CASP9 Ex5+32G>A (rs1052576) and CASP10 Ex3...


    pub_type: 杂志文章


    authors: Lan Q,Zheng T,Chanock S,Zhang Y,Shen M,Wang SS,Berndt SI,Zahm SH,Holford TR,Leaderer B,Yeager M,Welch R,Hosgood D,Boyle P,Rothman N

    更新日期:2007-04-01 00:00:00

  • Relationship between the depletion of O6-methylguanine-DNA methyltransferase by O6-methylguanine and the stimulation of DNA synthesis and growth of cultured chick hepatocytes.

    abstract::O6-Methylguanine-DNA methyltransferase (O6-MT) has been described as a DNA repair enzyme that reverses alkylation damage at the O6 position of guanine in DNA. We demonstrate that the concentration of this protein decreases immediately prior to DNA synthesis in cultured chick hepatocytes. If intracellular levels are ex...


    pub_type: 杂志文章


    authors: Dutta-Choudhury TA,Couri D,Stephens RE

    更新日期:1992-01-01 00:00:00

  • Slc10a2-null mice uncover colon cancer-promoting actions of endogenous fecal bile acids.

    abstract::Although epidemiological evidence in humans and bile acid feeding studies in rodents implicate bile acids as tumor promoters, the role of endogenous bile acids in colon carcinogenesis remains unclear. In this study, we exploited mice deficient in the ileal apical sodium-dependent bile acid transporter (ASBT, encoded b...


    pub_type: 杂志文章


    authors: Raufman JP,Dawson PA,Rao A,Drachenberg CB,Heath J,Shang AC,Hu S,Zhan M,Polli JE,Cheng K

    更新日期:2015-10-01 00:00:00

  • Lower levels of urinary 2-amino-3,8-dimethylimidazo[4,5-f]-quinoxaline (MeIQx) in humans with higher CYP1A2 activity.

    abstract::Heterocyclic aromatics amines (HAAs), such as 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), are metabolically activated by cytochrome P4501A2 (CYP1A2) and N-acetyltransferase (NAT2). We examined the relationship between CYP1A2 and NAT2 activity and the excretion of total unconjugated MeIQx in 66 healthy subje...


    pub_type: 杂志文章


    authors: Sinha R,Rothman N,Mark SD,Murray S,Brown ED,Levander OA,Davies DS,Lang NP,Kadlubar FF,Hoover RN

    更新日期:1995-11-01 00:00:00

  • Cyclin D1 gene polymorphism is associated with an increased risk of urinary bladder cancer.

    abstract::Cyclin D1 is believed to play an important role in the genesis and/or progression of transitional cell cancer (TCC) of the urinary bladder. Cyclin D1 gene (CCND1) mRNA is alternatively spliced to produce two transcripts, and the splicing pattern may be modulated by a G to A single nucleotide polymorphism within the sp...


    pub_type: 杂志文章


    authors: Wang L,Habuchi T,Takahashi T,Mitsumori K,Kamoto T,Kakehi Y,Kakinuma H,Sato K,Nakamura A,Ogawa O,Kato T

    更新日期:2002-02-01 00:00:00

  • Low expression of the Lsp1 gene in early mouse T-lymphomas induced by N-methyl-N-nitrosourea.

    abstract::The mouse Lsp1 gene encodes a 330 amino acid intracellular F-actin binding protein. Previously we showed that the mouse and human Lsp1 genes are expressed in normal B-cells and T-cells, including Thy1+ thymocytes and in normal macrophages and neutrophils. No or little LSP1 RNA and protein was found in a series of tran...


    pub_type: 杂志文章


    authors: Brennan L,Jongstra J

    更新日期:1996-04-01 00:00:00

  • Sequential and morphological analyses of aberrant crypt foci formation in mice of differing susceptibility to azoxymethane-induced colon carcinogenesis.

    abstract::Aberrant crypt foci (ACF), putative preneoplastic lesions, are early morphological changes induced by the colon carcinogen azoxymethane (AOM). Although inbred mice differ markedly in their susceptibility to AOM carcinogenesis, we have previously shown that ACF develop in both resistant and sensitive mouse strains afte...


    pub_type: 杂志文章


    authors: Papanikolaou A,Wang QS,Papanikolaou D,Whiteley HE,Rosenberg DW

    更新日期:2000-08-01 00:00:00

  • Short-term carcinogenesis bioassay of genotoxic procarcinogens in PIM transgenic mice.

    abstract::E mu-pim-1 transgenic mice, which overexpress the pim-1 oncogene in lymphoid tissues, have shown increased susceptibility to induction of T cell lymphomas by N-ethyl-N-nitrosourea, a direct-acting chemical carcinogen (Nature, 340, 61-63, 1989). We sought to further evaluate E mu-pim-1 transgenic mice as a potential te...


    pub_type: 杂志文章


    authors: Storer RD,Cartwright ME,Cook WO,Soper KA,Nichols WW

    更新日期:1995-02-01 00:00:00

  • Structure-activity relationships for epidermal ornithine decarboxylase induction and skin tumor promotion by anthrones.

    abstract::The present study was designed to compare the skin tumor promoting and epidermal ornithine decarboxylase (ODC) inducing activities of various structural analogs of anthralin (1,8-dihydroxy-9-anthrone) and chrysarobin (1,8-dihydroxy-3-methyl-9-anthrone). Groups of 30 SENCAR mice each were initiated with 7,12-dimethylbe...


    pub_type: 杂志文章


    authors: DiGiovanni J,Kruszewski FH,Coombs MM,Bhatt TS,Pezeshk A

    更新日期:1988-08-01 00:00:00

  • Selenium and the genesis of murine mammary tumors.

    abstract::One hundred forty-seven female Sprague-Dawley rats were divided into 5 groups and at 60 days of age were treated i.g. with 5 mg of 7,12-dimethylbenzanthracene (DMBA) suspended in 1.0 ml of sesame oil. Selenium (Se), as selenium dioxide (SeO2), was administered in the drinking water to 4 of the 5 groups (30 rats/group)...


    pub_type: 杂志文章


    authors: Welsch CW,Goodrich-Smith M,Brown CK,Greene HD,Hamel EJ

    更新日期:1981-01-01 00:00:00

  • Interaction of dietary fat and route of carcinogen administration on 1,2-dimethylhydrazine-induced colon tumorigenesis in rats.

    abstract::Since the results of an earlier study indicating no effect of dietary fat on dimethylhydrazine (DMH)-induced colon cancer in rats differed from those of other investigators, the present study was initiated to determine if the modulating effect of fat intake on colon tumorigenesis was dependent on the route of DMH admi...


    pub_type: 杂志文章


    authors: Locniskar M,Nauss KM,Kaufmann P,Newberne PM

    更新日期:1985-03-01 00:00:00

  • Breast carcinomas fulfill the Warburg hypothesis and provide metabolic markers of cancer prognosis.

    abstract::The aim of this study was to investigate selected proteomic markers of the metabolic phenotype of breast carcinomas as prognostic markers of cancer progression. For this purpose, a series of 101 breast carcinomas and 13 uninvolved breast samples were examined for quantitative differences in protein expression of mitoc...


    pub_type: 杂志文章


    authors: Isidoro A,Casado E,Redondo A,Acebo P,Espinosa E,Alonso AM,Cejas P,Hardisson D,Fresno Vara JA,Belda-Iniesta C,González-Barón M,Cuezva JM

    更新日期:2005-12-01 00:00:00

  • Expression of mutant thyroid hormone nuclear receptors is associated with human renal clear cell carcinoma.

    abstract::Thyroid hormone (T(3)) regulates proliferation and differentiation of cells, via its nuclear receptors (TRs). These processes have been shown to be abnormally regulated during carcinogenesis. We have previously found aberrant expression of TRalpha and TRbeta mRNAs in renal clear cell carcinoma (RCCC), suggesting possi...


    pub_type: 杂志文章


    authors: Kamiya Y,Puzianowska-Kuznicka M,McPhie P,Nauman J,Cheng SY,Nauman A

    更新日期:2002-01-01 00:00:00

  • Effect of modifying agents on the phenotypic expression of cytochrome P-450, glutathione S-transferase molecular forms, microsomal epoxide hydrolase, glucose-6-phosphate dehydrogenase and gamma-glutamyltranspeptidase in rat liver preneoplastic lesions.

    abstract::The expression of A and P forms of glutathione S-transferase (GST-A and P), two cytochrome P-450 isoenzymes (P-450 PB3a and P-450 MC2), microsomal epoxide hydrolase (mEHb), glucose-6-phosphate dehydrogenase (G6PD) and gamma-glutamyltranspeptidase (gamma-GT) was compared in preneoplastic liver lesions and background pa...


    pub_type: 杂志文章


    authors: Tsuda H,Moore MA,Asamoto M,Inoue T,Ito N,Satoh K,Ichihara A,Nakamura T,Amelizad Z,Oesch F

    更新日期:1988-04-01 00:00:00