Non-proteolytic activity of 19S proteasome subunit RPT-6 regulates GATA transcription during response to infection.

Abstract:

:GATA transcription factors play a crucial role in the regulation of immune functions across metazoans. In Caenorhabditis elegans, the GATA transcription factor ELT-2 is involved in the control of not only infections but also recovery after an infection. We identified RPT-6, part of the 19S proteasome subunit, as an ELT-2 binding partner that is required for the proper expression of genes required for both immunity against bacterial infections and recovery after infection. We found that the intact ATPase domain of RPT-6 is required for the interaction and that inhibition of rpt-6 affected the expression of ELT-2-controlled genes, preventing the appropriate immune response against Pseudomonas aeruginosa and recovery from infection by the pathogen. Further studies indicated that SKN-1, which is an Nrf transcription factor involved in the response to oxidative stress and infection, is activated by inhibition of rpt-6. Our results indicate that RPT-6 interacts with ELT-2 in vivo to control the expression of immune genes in a manner that is likely independent of the proteolytic activity of the proteasome.

journal_name

PLoS Genet

journal_title

PLoS genetics

authors

Olaitan AO,Aballay A

doi

10.1371/journal.pgen.1007693

subject

Has Abstract

pub_date

2018-09-28 00:00:00

pages

e1007693

issue

9

eissn

1553-7390

issn

1553-7404

pii

PGENETICS-D-18-00907

journal_volume

14

pub_type

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