Serological biomarkers of candidemia: a retrospective evaluation of three assays.


PURPOSE:Serologic testing allows for rapid detection of candidemia. More data are needed for the Virion\Serion ELISA antigen test (Ag), Hemkit Candida IHA antibody test (Ab), and Wako β-1,3-D-glucan assay (BDG). METHODS:Tests were performed on serum samples from 120 cases of culture-confirmed candidemia and 44 Candida-negative controls. Sensitivities and specificities of individual tests as well as combinations were assessed. RESULTS:The overall sensitivity of Ag, Ab, and Ag/Ab testing was 30, 40, and 54%, respectively, while in transplant patients it significantly dropped to 16, 26, and 40% (p = 0.02). For BDG testing it was 67%, both overall and in transplant patients. Especially Ag testing performed poorly among women ≤ 65 years with a significantly reduced sensitivity of 9% (p < 0.002). While the sensitivity of Ag/Ab testing was somewhat higher at 67% for C. albicans, it was significantly lower for non-albicans species at 42% (p = 0.006). The sensitivity of BDG testing for C. albicans and non-albicans species was not significantly different at 64 and 69%, respectively. Both Ag/Ab and BDG testing had a high specificity of 93%, for Ag testing it was 100%. Similar sensitivities were calculated for sera sampled on the day of and 4-6 days before sampling of positive blood cultures. CONCLUSIONS:Serological markers are valuable tools for the early diagnosis of candidemia. Ab, Ag, and BDG testing are all characterized by high specificity. The Wako BDG test is significantly more sensitive compared to combined Candida-Ag/Ab testing, particularly in the setting of non-albicans species and specific host factors.






Dichtl K,Seybold U,Wagener J




Has Abstract


2019-04-01 00:00:00














  • Postoperative cauda syndrome caused by Staphylococcus aureus.

    abstract::Toxic shock syndrome (TSS) is a well-defined clinical syndrome attributed to certain exotoxins produced by Staphylococcus aureus. The acute episode is often characterized by a toxic encephalopathy, possibly caused by direct neurotoxicity of these exotoxins, although this mechanism has never been proven. We describe a ...


    pub_type: 杂志文章


    authors: Arend SM,Steenmeyer AV,Mosmans PC,Bijlmer HA,van't Wout JW

    更新日期:1993-07-01 00:00:00

  • Nosocomial pneumonia caused by carbapenem-resistant Raoultella planticola: a case report and literature review.

    abstract::Raoultella planticola is a rare opportunistic pathogen usually invaded immunocompromised patients and sometimes even causes fatal infections. Recently, there is growing concern about the emergence of carbapenem resistance in this species. Here, we describe one case of hospital-acquired pneumonia due to a carbapenem-re...


    pub_type: 杂志文章,评审


    authors: Xu M,Xie W,Fu Y,Zhou H,Zhou J

    更新日期:2015-04-01 00:00:00

  • Using the kinetics of C-reactive protein response to improve the differential diagnosis between acute bacterial and viral infections.

    abstract:PURPOSE:Differential diagnosis between acute viral and bacterial infection is an emerging common challenge for a physician in the emergency department. Serum C-reactive protein (CRP) is used to support diagnosis of bacterial infection, but in patients admitted with low CRP, its ability to discriminate between viral and...


    pub_type: 杂志文章


    authors: Coster D,Wasserman A,Fisher E,Rogowski O,Zeltser D,Shapira I,Bernstein D,Meilik A,Raykhshtat E,Halpern P,Berliner S,Shenhar-Tsarfaty S,Shamir R

    更新日期:2020-04-01 00:00:00

  • [Comparative study of cefaclor versus amoxicillin in urinary tract infections (author's transl)].

    abstract::In a comparative, prospective, open randomised clinical study, the efficacy and tolerance of cefaclor (3 x 500 mg) and amoxicillin (3 x 750 mg) in the treatment of urinary tract infection (UTI) were investigated and compared. A total of 110 patients (51 males and 59 females) age 10 to 83 years were treated for a perio...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Naber K,Kaldewey W

    更新日期:1979-01-01 00:00:00

  • Enterococcus faecalis endocarditis presenting as meningitis.

    abstract::Although Enterococcus faecalis is a relatively common cause of infective endocarditis, it rarely causes meningitis. A case of Enterococcus faecalis endocarditis presenting as meningitis in a 74-year-old diabetic man on chronic hemodialysis is reported. A review of the literature showed that the association of enteroco...


    pub_type: 杂志文章


    authors: Lin DP,Wada S,Jimenez-Lucho VE

    更新日期:1998-09-01 00:00:00

  • Protective effect of an outer membrane vaccine against Pseudomonas aeruginosa infection.

    abstract::The outer membrane proteins, F, H2 and I, from PA12 strain of Pseudomonas aeruginosa, were purified and used successfully for immunization of Balb/c mice. The immunized mice were protected against challenge with a 2-26 fold LD50 of all known 19 serogroups of P. aeruginosa. ...


    pub_type: 杂志文章


    authors: von Specht BU,Strigl G,Ehret W,Brendel W

    更新日期:1987-01-01 00:00:00

  • Lymphocyte apoptosis, caspase activation and inflammatory response in septic shock.

    abstract:BACKGROUND:We examined lymphocyte apoptosis, activity of caspases-1, -3, -8 and -9 and the relationship between those two events and inflammation response in septic shock. MATERIALS AND METHODS:Blood samples were obtained within 24 h after diagnosis of septic shock from 16 patients to measure apoptosis, caspases-3, -8...


    pub_type: 信件


    authors: Delogu G,Famularo G,Tellan G,Marandola M,Antonucci A,Signore M,Marcellini S,Moretti S

    更新日期:2008-10-01 00:00:00

  • Cefodizime given once daily for the treatment of upper urinary tract infections and complicated lower urinary tract infections.

    abstract::The efficacy of cefodizime (CDZ) in upper urinary tract infections (UUTI) and complicated lower urinary tract infections (LUTI) was assessed by analysis of the combined results of five clinical trials. Doses of CDZ 1 g i.m. daily, 1 x 2 g i.v. or i.m. and 2 x 2 g i.v. were investigated; comparative agents were cefurox...


    pub_type: 杂志文章


    authors: Hofstetter AG

    更新日期:1992-01-01 00:00:00

  • [Microbiological diagnosis of septicemia].

    abstract::To detect microorganisms in the blood it is necessary not only that the microbiologist uses reliable methods, but also that the clinician takes a sufficient number of blood samples at the right point in time using a correct method for drawing the blood. The best results are obtained if the blood sample is transferred ...


    pub_type: 杂志文章


    authors: Rotter M

    更新日期:1977-03-01 00:00:00

  • Automated ribotyping and pulsed-field gel electrophoresis reveal a cluster of group A streptococci in intravenous drug abusers.

    abstract:BACKGROUND:In 1998, an unexpected increase of group A streptococci (GAS) in blood cultures was observed at our institution. MATERIALS AND METHODS:To determine whether they were from unrelated sporadic cases or attributable to a common source, all isolates were analyzed by pulsed-field gel electrophoresis (PFGE) and by...


    pub_type: 杂志文章


    authors: Brunner S,Fleisch F,Ruef C,Altwegg M

    更新日期:2000-09-01 00:00:00

  • Characteristics and outcome of 16 periprosthetic shoulder joint infections.

    abstract:PURPOSE:Shoulder arthroplasties are increasingly performed, but data on periprosthetic joint infections (PJI) in this anatomical position are limited. We retrospectively investigated the characteristics and outcome of shoulder PJI after primary arthroplasty from 1998 to 2010 in a single centre. METHODS:Periprosthetic ...


    pub_type: 杂志文章


    authors: Achermann Y,Sahin F,Schwyzer HK,Kolling C,Wüst J,Vogt M

    更新日期:2013-06-01 00:00:00

  • Deep sternal wound infection due to Pasteurella multocida: the first case report and review of literature.

    abstract::Pasteurella multocida is a Gram-negative bacterium recovered from a wide variety of wild and domestic animals and has mostly been associated with infection following animal bites. We present the first reported case of a patient who developed a postoperative sternal wound infection due to P. multocida complicated by bl...


    pub_type: 杂志文章,评审


    authors: Baillot R,Voisine P,Côté LM,Longtin Y

    更新日期:2011-12-01 00:00:00

  • The course of cryptococcal capsular polysaccharide antigenemia/human cryptococcal polysaccharide elimination kinetics.

    abstract::The detection of cryptococcal polysaccharides in the serum is diagnostic of cryptococcosis in the absence of rheumatoid factor. The significance of the continued detection of this antigen in the serum during antifungal therapy is not known. Prolonged antigenemia might indicate ongoing active infection, delayed clearan...


    pub_type: 杂志文章


    authors: Eng R,Chmel H,Corrado M,Smith SM

    更新日期:1983-05-01 00:00:00

  • In-vitro activity of lomefloxacin (SC-47111) and other quinolones.

    abstract::The in vitro activity of lomefloxacin, a new difluorinated quinolone, was compared to that of ciprofloxacin, ofloxacin, norfloxacin and other relevant antibacterial agents. Lomefloxacin and norfloxacin shared similar activity, whereas ofloxacin and in particular ciprofloxacin showed superior activity. Most gram-negati...


    pub_type: 杂志文章


    authors: Sun ZM,Maskell JP,Sehgal SC,Williams JD

    更新日期:1989-05-01 00:00:00

  • Basis and results of therapy with Beta-lactam antibiotics in experimental infections.

    abstract::Experimental infections in animals and their chemotherapy are dependent on a variety of experimental conditions and parameters which may be themselves interrelated; the type and number of infecting organisms, the nature of the infection, the antibiotic chosen for therapy and its administration schedule can influence t...


    pub_type: 杂志文章


    authors: Rolinson GN

    更新日期:1980-01-01 00:00:00

  • M. pneumoniae and C. pneumoniae are no relevant pathogens in critically ill patients with hospital-acquired respiratory tract infections.

    abstract:PURPOSE:To assess the incidence of Mycoplasma pneumoniae and Chlamydia pneumoniae in the pathogenesis of hospital-acquired respiratory tract infections (RTIs) in critically ill patients. METHODS:This is a retrospective cohort study of all ICU-patients ≥ 18 years with RTI who underwent conventional culture techniques a...


    pub_type: 杂志文章


    authors: Hagel S,Schmitt S,Kesselmeier M,Baier M,Welte T,Ewig S,Pletz MW

    更新日期:2019-06-01 00:00:00

  • Comparative study of the binding of acylureidopenicillins and carbenicillin to human serum proteins.

    abstract::The binding of the acylureidopenicillins azlocillin and mezlocillin to serum proteins was investigated by means of equilibrium dialysis and ultracentrifugation. The penicillin concentrations were determined by the agar diffusion test and by circular dichroism. The degree of binding is dependent on the penicillin conce...


    pub_type: 杂志文章


    authors: Rosenkranz H,Förster D

    更新日期:1979-01-01 00:00:00

  • Localized pulmonary infection associated with Mycobacterium tilburgii in an HIV-infected patient.

    abstract::The case of an HIV-infected patient with low CD4+ cell count and localized pulmonary infection associated with Mycobacterium tilburgii, a recently recognized atypical mycobacterial species, is reported. Diagnosis was confirmed by repeated detection of mycobacterial DNA in lung biopsy specimens. After surgical removal ...


    pub_type: 杂志文章


    authors: Kolditz M,Halank M,Spornraft-Ragaller P,Schmidt H,Höffken G

    更新日期:2005-08-01 00:00:00

  • Influence of intimal Chlamydophila pneumoniae persistence on cardiovascular complications after coronary intervention.

    abstract:PURPOSE:Chlamydophila pneumoniae has been implicated in atherosclerosis/restenosis; however, clear evidence is missing. Therefore, the aim of our study was to examine the influence of intimal infection and systemic inflammation on cardiovascular complications after coronary intervention. METHODS:45 atheroma specimens ...


    pub_type: 杂志文章


    authors: Tuleta I,Reek D,Braun P,Bauriedel G,Nickenig G,Skowasch D,Andrié R

    更新日期:2015-02-01 00:00:00

  • Controversies in the therapeutic approach to congenital cytomegalovirus infection.

    abstract::Treatment of congenital cytomegalovirus infection is mandatory in cases with severe systemic and/or neurological involvement. However, some patients are paucisymptomatic, with very subtle systemic manifestations and/or minimal brain alterations. Current international guidelines do not clearly state whether these child...


    pub_type: 杂志文章


    authors: Garrido-Barbero M,Arnaez J,Garcia-Alix A

    更新日期:2020-06-01 00:00:00

  • Promoting Global Research Excellence in Severe Sepsis (PROGRESS): lessons from an international sepsis registry.

    abstract:BACKGROUND:The PROGRESS Registry (Promoting Global Research Excellence in Severe Sepsis) was designed to provide comparative data reflecting everyday clinical practice, thereby allowing participating institutions to explore and benchmark medical interventions in severe sepsis. MATERIALS AND METHODS:PROGRESS was an int...


    pub_type: 杂志文章,多中心研究


    authors: Beale R,Reinhart K,Brunkhorst FM,Dobb G,Levy M,Martin G,Martin C,Ramsey G,Silva E,Vallet B,Vincent JL,Janes JM,Sarwat S,Williams MD,PROGRESS Advisory Board.

    更新日期:2009-06-01 00:00:00

  • ELISA for antibodies to lipid A, lipopolysaccharides and other hydrophobic antigens.

    abstract::A simple, sensitive and rapid ELISA method for the quantification and characterization of antibodies to lipid A has been developed, which can also be applied to other hydrophobic antigens. For coating, antigens were applied to the wells of ELISA plates as solutions in a mixture of chloroform and ethanol 1:9 (v/v), and...


    pub_type: 杂志文章


    authors: Freudenberg MA,Fomsgaard A,Mitov I,Galanos C

    更新日期:1989-09-01 00:00:00

  • Candidemia in intensive care unit patients: risk factors for mortality.

    abstract::Aim of this study was to evaluate whether risk factors which predict the development of candidemia may also predict death in ICU patients with candidemia. During an 8-year-period all ICU patients whose blood cultures yielded Candida species (n = 40) were retrospectively evaluated in a case-control fashion. The average...


    pub_type: 杂志文章


    authors: Voss A,le Noble JL,Verduyn Lunel FM,Foudraine NA,Meis JF

    更新日期:1997-01-01 00:00:00

  • Clearance of Candida colonizing the urinary bladder by a two-day amphotericin B irrigation.

    abstract::The minimum duration of continuous amphotericin B irrigation (50 mg/liter/day) required to clear the candida colonizing the urinary bladder was investigated. Eleven in vitro experiments revealed a consistent candidicidal effect within 48 hours with 25 and 5 mg/l of amphotericin B concentrations attainable in the bladd...


    pub_type: 杂志文章


    authors: Hsu CC,Ukleja B

    更新日期:1990-09-01 00:00:00

  • Plasmid fingerprinting of methicillin-resistant Staphylococcus aureus strains isolated in Hamburg.

    abstract::By means of restriction endonuclease digests and DNA/hybridisation studies we analysed ten representative methicillin-resistant Staphylococcus aureus strains of our collection for plasmid similarities and plasmid associated resistance determinants. We found that strains isolated at our laboratory contained identical o...


    pub_type: 杂志文章


    authors: Gatermann S

    更新日期:1987-11-01 00:00:00

  • Serum immunoglobulin levels in hepatitis non-A, non-B: a comparison with hepatitis A and B.

    abstract::The serum levels of IgA, IgG and IgM were determined by the single radial immunodiffusion method in 86 patients with acute viral hepatitis serologically differentiated by radioimmunoassays. The levels of IgA and IgG were similar in patients with hepatitis A, B and non-A, non-B, while differences in IgM levels were obs...


    pub_type: 杂志文章


    authors: Norkrans G,Nilsson LA,Frösner G,Wahl M,Iwarson S

    更新日期:1980-01-01 00:00:00

  • Clindamycin-induced hypersensitivity reaction.

    abstract::Drug-induced anaphylaxis is an unpredictable adverse reaction. Although it may occur with any medication, antibiotics induce more cases of anaphylaxis than any other medication class with most cases being induced by β-lactam antibiotics. Clindamycin is an antibiotic with good gram-positive and anaerobe coverage which ...


    pub_type: 杂志文章


    authors: Bulloch MN,Baccas JT,Arnold S

    更新日期:2016-06-01 00:00:00

  • [The antibacterial efficacy of cefaclor in routine testing of clinical material from two Berlin hospitals (author's transl)].

    abstract::The agar diffusion method was used to test the antibacterial efficacy of cefaclor against bacterial strains isolated routinely from patients in two hospitals in Berlin. A comparison was made with the efficacy of oxacillin, azlocillin, amikacin, gentamicin, ampicillin, co-trimoxazole, tetracycline, penicillin, cefazoli...


    pub_type: 杂志文章


    authors: Wagner J,Hahn H

    更新日期:1979-01-01 00:00:00

  • A retrospective analysis of weight changes in HIV-positive patients switching from a tenofovir disoproxil fumarate (TDF)- to a tenofovir alafenamide fumarate (TAF)-containing treatment regimen in one German university hospital in 2015-2017.

    abstract:PURPOSE:To determine whether changing from a tenofovir disoproxil fumarate (TDF)- to a tenofovir alafenamide fumarate (TAF)-containing regimen is correlated with weight changes in a human immunodeficiency virus (HIV)-positive adult cohort. METHODS:Retrospective analysis was conducted of data gathered from routine care...


    pub_type: 杂志文章


    authors: Gomez M,Seybold U,Roider J,Härter G,Bogner JR

    更新日期:2019-02-01 00:00:00

  • Passive immunization against cytomegalovirus in allograft recipients. The Rotterdam Heart Transplant Program experience.

    abstract::We analyzed the results of passive immunization against CMV in 146 heart transplant recipients. The 65 seronegative recipients were prophylactically treated with anti-CMV immunoglobulins during and after the operation. Twenty-nine of these 65 patients received a seropositive donor heart. CMV infection occurred in 21/6...


    pub_type: 杂志文章


    authors: Balk AH,Weimar W,Rothbarth PH,Meeter K,Metselaar HJ,Mochtar B,Simoons ML

    更新日期:1993-07-01 00:00:00