Upregulation of microRNA-129-5p inhibits cell invasion, migration and tumor angiogenesis by inhibiting ZIC2 via downregulation of the Hedgehog signaling pathway in cervical cancer.


:Recently, some studies have placed additional research focus on microRNAs (miRNAs) in a bid to discover novel therapeutic approaches for cervical cancer (CC), which is one of the most common female reproductive tract malignancies with high rates of morbidity and mortality. Hence, the aim of the present study was to evaluate the ability of miR-129-5p to influence cell angiogenesis, invasion and migration by targeting ZIC2 through the Hedgehog signaling pathway in CC. Both CC and adjacent normal tissues were extracted from 87 eligible participating patients with CC. Measurements of the levels of miR-129-5p, mRNA and protein levels of ZIC2, sonic Hedgehog (Shh), Gli1, and Gli2 and levels of CXCL1, VEGF and Ang2 were determined accordingly. An angiogenesis assay was performed to evaluate cell angiogenesis in vitro, while a scratch test and transwell assay were adopted for cell invasion and migration determination. Lastly, tumor formation within nude mice was performed in order to analyze angiogenesis and tumor growth among the nude mice in vivo. The findings revealed that upregulation of miR-129-5p resulted in the decrease in the mRNA and protein levels of ZIC2, Shh, Gli1, Gli2, as well as reduced levels of CXCL1, VEGF and Ang2. Moreover, up-regulation of miR-129-5p was determined to inhibit CC cell angiogenesis ability in vitro, in addition to the processes of cell migration, and invasion. Finally, up-regulation of miR-129-5p was observed to inhibit the tumor growth and angiogenesis ability of nude mice in vivo. The results of the present study provided evidence suggesting that overexpressed miR-129-5p prevents angiogenesis and inhibits cell migration and invasion by means of negatively targeting ZIC2 through suppression of the Hedgehog signaling pathway in CC. Thus, highlighting the promise of miR-129-5p as a novel target for treating CC is promising.


Cancer Biol Ther


Cancer biology & therapy


Wang YF,Yang HY,Shi XQ,Wang Y




Has Abstract


2018-01-01 00:00:00












  • Molecular functions of BRCA1 in the DNA damage response.

    abstract::Ten years ago, a concerted effort from several labs resulted in the cloning of BRCA1, the first of two major hereditary breast/ovarian cancer predisposition genes. Since that time, BRCA1 has been linked to several key nuclear functions connected with the prevention of genomic instability. In particular, BRCA1 function...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Scully R,Xie A,Nagaraju G

    更新日期:2004-06-01 00:00:00

  • The combination of 5-fluorouracil plus p53 pathway restoration is associated with depletion of p53-deficient or mutant p53-expressing putative colon cancer stem cells.

    abstract::The cancer stem cell hypothesis suggests that rare populations of tumor-initiating cells may be resistant to therapy, lead to tumor relapse and contribute to poor prognosis for cancer patients. We previously demonstrated the feasibility of p53 pathway restoration in p53-deficient tumor cell populations using small mol...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Huang C,Zhang XM,Tavaluc RT,Hart LS,Dicker DT,Wang W,El-Deiry WS

    更新日期:2009-11-01 00:00:00

  • Differential impact of fibroblasts on the efficient cell death of lung cancer cells induced by paclitaxel and cisplatin.

    abstract::The efficient treatment of lung carcinomas with chemotherapeutics still poses a challenge for anti-cancer therapy. Since stromal cells of the tumor may alter the responsiveness of tumor cells to chemotherapeutics, we studied the impact of lung fibroblasts (WI-38) on the chemotherapy-induced death of non-small cell lun...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Bartling B,Hofmann HS,Silber RE,Simm A

    更新日期:2008-08-01 00:00:00

  • Recurrent gliomas: comparison of computed tomography (CT)-guided 125I seed implantation therapy and traditional radiochemotherapy.

    abstract:BACKGROUND:Primary brain tumors have always been associated with high morbidity and mortality. Glioma is the most common type of malignant brain tumors,with a high probability of recurrence after surgical excision and with poor prognosis.The purpose of this study was to compare the therapeutic efficacy of computed tomo...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Hu X,Qiu H,Zhang L,Zhang W,Ma Y,Qiao Z,Chen D,Han J,Duan G,Zhang F

    更新日期:2012-08-01 00:00:00

  • Absence of SV40 in Austrian tumors correlates with low incidence of mesotheliomas.

    abstract::Between 1955 and 1963 millions of people were worldwide vaccinated with polio-vaccines that were contaminated with the simian virus 40 (SV40). This tumor-inducing virus has subsequently been detected in several human tumors. In Austria, polio mass vaccination started in winter 1961/62 with a presumably SV40-free Briti...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Leithner A,Weinhaeusel A,Windhager R,Schlegl R,Waldner P,Lang S,Dominkus M,Zoubek A,Popper HH,Haas OA

    更新日期:2002-07-01 00:00:00

  • Molecular basis of pathogenesis, prognosis and therapy in chronic lymphocytic leukaemia.

    abstract::B-cell chronic lymphocytic leukemia (CLL) is caused by the abnormal accumulation of non-functional B-cells in peripheral blood and bone marrow. However, the precise aetiology and mechanism of the disease are unclear. Recently, progress has been made in the identification of both the genetic deficiencies and environmen...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Chen J,McMillan NA

    更新日期:2008-02-01 00:00:00

  • Faecal microflora and beta-glucuronidase expression are altered in an irinotecan-induced diarrhea model in rats.

    abstract:OBJECTIVES:Chemotherapy-induced diarrhea (CID) is a well recognized side effect of cancer treatment. However, the pathophysiology behind this debilitating side effect remains unclear. Irinotecan causes cholinergic and delayed onset diarrhea in patients, in which beta-glucuronidase produced by gut bacteria is thought to...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Stringer AM,Gibson RJ,Logan RM,Bowen JM,Yeoh AS,Keefe DM

    更新日期:2008-12-01 00:00:00

  • Gefitinib-sensitizing mutation in esophageal carcinoma cell line Kyse450.

    abstract:PURPOSE:The sensitivity of lung cancer to gefitinib has been found to be associated with mutations at the tyrosine kinase domain of epidermal growth factor receptor (EGFR), yet similar observations are not available in other solid tumors. We recently identified mutations in the EGFR kinase domain in primary esophageal ...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Guo M,Liu S,Herman JG,Zhuang H,Lu F

    更新日期:2006-02-01 00:00:00

  • MiR-101 inhibits cell growth and tumorigenesis of Helicobacter pylori related gastric cancer by repression of SOCS2.

    abstract::Several microRNAs (miRNA) have been implicated in H. pylori related gastric cancer (GC). However, the molecular mechanism of miRNAs in gastric cancer has not been fully understood. In this study, we reported that miR-101 is significantly down-regulated in H. pylori positive tissues and cells and in tumor tissues with ...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Zhou X,Xia Y,Li L,Zhang G

    更新日期:2015-01-01 00:00:00

  • Detection of apoptotic circulating tumor cells in advanced pancreatic cancer following 5-fluorouracil chemotherapy.

    abstract::The objective of the present study was to evaluate CA19-9 and CK8/18 expression patterns in pancreatic cancer cell lines induced by 5-fluorouracil (5-Fu), and in circulating tumor cells (CTCs) in peripheral blood of patients previously untreated with advanced pancreatic cancer. Furthermore, the goal was to test the re...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Ren C,Han C,Zhang J,He P,Wang D,Wang B,Zhao P,Zhao X

    更新日期:2011-10-15 00:00:00

  • Paclitaxel-induced FasL-independent apoptosis and slow (non-apoptotic) cell death.

    abstract::Microtubule-active drugs, including paclitaxel (Taxol, PTX), cause mitotic arrest, and this can result in apoptosis. A recently study has reported that PTX mediates apoptosis by upregulating FasL in Jurkat and MDA-231 cells. In contrast to the previous report, we found that anti-FasL antibodies failed to inhibit PTX-i...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Blagosklonny MV,Robey R,Sheikh MS,Fojo T

    更新日期:2002-03-01 00:00:00

  • C1B domain peptide of protein kinase Cγ significantly suppresses growth of human colon cancer cells in vitro and in an in vivo mouse xenograft model through induction of cell cycle arrest and apoptosis.

    abstract::Two peptides derived from the C1B domain of protein kinase Cγ (PKCγ) were shown to associate with classical PKC isozymes and modulate their activities. These C1B peptides are designated C1B1 (amino acid residues 101-112) and C1B5 (residues 141-151). Since PKC enzyme activity is shown to be involved in colon cancer dev...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Kawabata A,Matsuzuka T,Doi C,Seiler G,Reischman J,Pickel L,Ayuzawa R,Nguyen TA,Tamura M

    更新日期:2012-08-01 00:00:00

  • Epigenetic changes activate widespread signals in response to double-strand breaks.

    abstract::Double-strand breaks are one the most severe types of DNA damage with respect to cell survival and the preservation of genomic integrity. Therefore, cells have evolved complex mechanisms including cell cycle regulation, activation of repair pathways and in certain cases induction of apoptosis in response to these lesi...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Karagiannis TC,El-Osta A

    更新日期:2004-07-01 00:00:00

  • Aggressive myeloid leukemia formation is directed by the Musashi 2/Numb pathway.

    abstract::Chronic myeloid leukemia (CML) progresses from a chronic phase to a deadly blast crisis phase. While it is known that BCR-ABL initiates the disease and that secondary molecular and genetic abnormalities likely contribute to progression of the disease to blast crisis, details regarding the mechanism(s) of blast phase p...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Griner LN,Reuther GW

    更新日期:2010-11-15 00:00:00

  • Analysis and significance of c-MET expression in adenoid cystic carcinoma of the salivary gland.

    abstract::Adenoid cystic carcinoma (ACC), a rare salivary gland malignancy, is a histogenetic, morphologic, and clinical heterogeneous disease. Extensive efforts have been made to characterize molecular events associated with these tumors, including the identification of prognostic and predictive biomarkers. Increased copy numb...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Bell D,Ferrarotto R,Fox MD,Roberts D,Hanna EY,Weber RS,El-Naggar AK

    更新日期:2015-01-01 00:00:00

  • A homologous promoterless K-ras cDNA targeting endogenous K-ras expression inhibits human pancreatic cancer cell growth in vitro and in vivo.

    abstract::It has been reported that the local introduction of a promoter-less DNA containing the complementary DNA (cDNA) sequence of a gene could induce gene-specific silencing in plants. The feasibility of this kind of silencing in human cancer cells is as yet unknown. The current study was designed to investigate the anti-tu...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Ren XY,Liang ZY,Shi XH,Liu TH

    更新日期:2008-10-01 00:00:00

  • A DNA repeat, NBL2, is hypermethylated in some cancers but hypomethylated in others.

    abstract::Hypermethylation at certain CpG-rich promoters and hypomethylation at repeated DNA sequences are very frequently found in cancers. We provide the first report that a DNA sequence (NBL2) can be either extensively hypermethylated or hypomethylated in cancer. Previously, it was shown that NBL2, a complex tandem DNA repea...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Nishiyama R,Qi L,Tsumagari K,Weissbecker K,Dubeau L,Champagne M,Sikka S,Nagai H,Ehrlich M

    更新日期:2005-04-01 00:00:00

  • Cost-effectiveness of RAS screening before monoclonal antibodies therapy in metastatic colorectal cancer based on FIRE3 Study.

    abstract::The surprising results published by FIRE-3 revealed that the overall survival (OS) of RAS wild-type metastatic colorectal cancer (mCRC) patients treated with Cetuximab(Cmab) and FOLFIRI combination was prolonged to 33.1 months. The substantial increase in testing and treatment costs, however, impose a considerable hea...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Wen F,Yang Y,Zhang P,Zhang J,Zhou J,Tang R,Chen H,Zheng H,Fu P,Li Q

    更新日期:2015-01-01 00:00:00

  • Therapeutic effect of arsenic trioxide (As2O3) on cervical cancer in vitro and in vivo through apoptosis induction.

    abstract::Arsenic trioxide (As2O3) induces apoptosis in certain types of cancer cells. But the detailed mechanisms of As2O3 efficacy are not completely known. Here we demonstrate that As2O3 has a therapeutic effect on cervical cancer in vitro and in vivo. We investigated the As2O3-induced apoptosis in various cervical cancer ce...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Yu J,Qian H,Li Y,Wang Y,Zhang X,Liang X,Fu M,Lin C

    更新日期:2007-04-01 00:00:00

  • Intravenous liposomal delivery of the short hairpin RNAs against Plk1 controls the growth of established human hepatocellular carcinoma.

    abstract::Polo-like kinase 1 (Plk1) is a key cell cycle regulator that is frequently overexpressed in human hepatocellular carcinomas. Blockade of the Plk1 pathway has been reported to be capable of inducing anti-tumor effect. Here, plasmids containing U6 promoter-driven shRNAs against human Plk1 were constructed and transfecte...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Deng H,Jiang Q,Yang Y,Zhang S,Ma Y,Xie G,Chen X,Qian Z,Wen Y,Li J,Yang J,Chen L,Zhao X,Wei Y

    更新日期:2011-02-15 00:00:00

  • New onset acute promyelocytic Leukemia during pregnancy: report of 2 cases.

    abstract::Acute promyelocytic leukemia (APL) is one of the most fatal hematological malignancies. APL during pregnancy is a rare comorbidity and can lead to adverse outcomes, such as maternal and/or fetal death, without timely and appropriate management. Medical management for APL during pregnancy remains challenging. We report...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Li H,Han C,Li K,Li J,Wang Y,Xue F

    更新日期:2019-01-01 00:00:00

  • Silencing of heparanase by siRNA inhibits tumor metastasis and angiogenesis of human breast cancer in vitro and in vivo.

    abstract::Expression of the heparanase gene is associated with invasive, angiogenic and metastatic potential of diverse malignant tumors and cell lines. Here we used RNA interference strategies to evaluate the role of human heparanase in breast malignancy and to explore the therapeutic potential of its specific targeting. The s...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Zhang ZH,Chen Y,Zhao HJ,Xie CY,Ding J,Hou YT

    更新日期:2007-04-01 00:00:00

  • Translational control of gene expression during hypoxia.

    abstract::Poor oxygenation is a unique and prevalent feature of solid tumors associated with poor patient prognosis. In part, this is caused by a series of adaptive cellular responses that together have a large impact on gene expression and cell phenotype. HIF plays a key role in this response by activating a transcriptional pr...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: van den Beucken T,Koritzinsky M,Wouters BG

    更新日期:2006-07-01 00:00:00

  • Targeting c-Met in melanoma: mechanism of resistance and efficacy of novel combinatorial inhibitor therapy.

    abstract::Numerous tyrosine kinase inhibitors (TKIs) targeting c-Met are currently in clinical trials for several cancers. Their efficacy is limited due to the development of resistance. The present study aims to elucidate this mechanism of c-Met TKI resistance by investigating key mTOR and Wnt signaling proteins in melanoma ce...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Etnyre D,Stone AL,Fong JT,Jacobs RJ,Uppada SB,Botting GM,Rajanna S,Moravec DN,Shambannagari MR,Crees Z,Girard J,Bertram C,Puri N

    更新日期:2014-09-01 00:00:00

  • Systemic and skin toxicity in Cercopithecus aethiops sabaeus monkeys treated during 26 weeks with a high intravenous dose of the anti- epidermal growth factor receptor monoclonal antibody Nimotuzumab.

    abstract::Nimotuzumab (h-R3) is a humanized anti-epidermal growth factor receptor monoclonal antibody (mAb) registered for treating head and neck tumours. The present study was designed to evaluate the systemic and skin toxicity of chronic intravenous administration of the h-R3 in a relevant species demonstrated by comparing th...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Arteaga ME,Ledón N,Casacó A,Pardo B,García M,Boleda M,Viña L,Orphee R,Hernández O,González C,Fuentes D,Rodríguez V,Charro L,Baro F,Macías A,Pérez A,Morales Y,Subirós N,González B,Ramos M,Rodriquez L,Ballester-La

    更新日期:2007-09-01 00:00:00

  • Abscopal effect of metastatic pancreatic cancer after local radiotherapy and granulocyte-macrophage colony-stimulating factor therapy.

    abstract::The role of immunotherapy in combination with traditional treatment regime in improving the survival of cancer patients has attracted more and more attention. Especially the abscopal effect that describes the phenomenon of localized radiotherapy leading to regression of distant unirradiated tumors and the role of enha...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Shi F,Wang X,Teng F,Kong L,Yu J

    更新日期:2017-03-04 00:00:00

  • Tissue-based immune monitoring II: multiple tumor sites reveal immunologic homogeneity in serous ovarian carcinoma.

    abstract::The presence of tumor-infiltrating lymphocytes (TILs) in epithelial ovarian cancer indicates a host antitumor response and is associated with improved survival. We wished to determine the extent to which TIL density differs from site to site within a given patient. We initially studied multiple paired metastases from ...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Hagemann AR,Hagemann IS,Cadungog M,Hwang WT,Patel P,Lal P,Hammond R,Gimotty PA,Chu CS,Rubin SC,Birrer MJ,Powell DJ Jr,Feldman MD,Coukos G

    更新日期:2011-08-15 00:00:00

  • Deletion of MBD2 inhibits proliferation of chronic myeloid leukaemia blast phase cells.

    abstract::Aberrant methylation of tumour suppressor genes is associated with the progression to a blast crisis in chronic myeloid leukaemia (CML). Methyl-CpG-binding domain protein 2 (MBD2) has been studied as a "reader" of DNA methylation in many cancers, but its role in CML is unclear. We constructed cell models of a homozygo...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Cheng L,Tang Y,Chen X,Zhao L,Liu S,Ma Y,Wang N,Zhou K,Zhou J,Zhou M

    更新日期:2018-08-03 00:00:00

  • HPV epigenetic mechanisms related to Oropharyngeal and Cervix cancers.

    abstract::Human Papilloma Virus infection is very frequent in humans and is mainly transmitted sexually. The majority of infections are transient and asymptomatic, however, if the infection persists, it can occur with a variety of injuries to skin and mucous membranes, depending on the type of HPV involved. Some types of HPV ar...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Di Domenico M,Giovane G,Kouidhi S,Iorio R,Romano M,De Francesco F,Feola A,Siciliano C,Califano L,Giordano A

    更新日期:2018-01-01 00:00:00

  • RNA interference of MBD1 in BxPC-3 human pancreatic cancer cells delivered by PLGA-poloxamer nanoparticles.

    abstract::Methyl-CpG binding domain protein 1 (MBD1) is a transcriptional regulator that binds methylated CpG islands of tumor suppressor genes and represses their transcription. In a former study, we found high expression of MBD1 in pancreatic cancer cell lines and tissues which may play an important role in the development of...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Luo G,Jin C,Long J,Fu D,Yang F,Xu J,Yu X,Chen W,Ni Q

    更新日期:2009-04-01 00:00:00