Upregulation of microRNA-129-5p inhibits cell invasion, migration and tumor angiogenesis by inhibiting ZIC2 via downregulation of the Hedgehog signaling pathway in cervical cancer.


:Recently, some studies have placed additional research focus on microRNAs (miRNAs) in a bid to discover novel therapeutic approaches for cervical cancer (CC), which is one of the most common female reproductive tract malignancies with high rates of morbidity and mortality. Hence, the aim of the present study was to evaluate the ability of miR-129-5p to influence cell angiogenesis, invasion and migration by targeting ZIC2 through the Hedgehog signaling pathway in CC. Both CC and adjacent normal tissues were extracted from 87 eligible participating patients with CC. Measurements of the levels of miR-129-5p, mRNA and protein levels of ZIC2, sonic Hedgehog (Shh), Gli1, and Gli2 and levels of CXCL1, VEGF and Ang2 were determined accordingly. An angiogenesis assay was performed to evaluate cell angiogenesis in vitro, while a scratch test and transwell assay were adopted for cell invasion and migration determination. Lastly, tumor formation within nude mice was performed in order to analyze angiogenesis and tumor growth among the nude mice in vivo. The findings revealed that upregulation of miR-129-5p resulted in the decrease in the mRNA and protein levels of ZIC2, Shh, Gli1, Gli2, as well as reduced levels of CXCL1, VEGF and Ang2. Moreover, up-regulation of miR-129-5p was determined to inhibit CC cell angiogenesis ability in vitro, in addition to the processes of cell migration, and invasion. Finally, up-regulation of miR-129-5p was observed to inhibit the tumor growth and angiogenesis ability of nude mice in vivo. The results of the present study provided evidence suggesting that overexpressed miR-129-5p prevents angiogenesis and inhibits cell migration and invasion by means of negatively targeting ZIC2 through suppression of the Hedgehog signaling pathway in CC. Thus, highlighting the promise of miR-129-5p as a novel target for treating CC is promising.


Cancer Biol Ther


Cancer biology & therapy


Wang YF,Yang HY,Shi XQ,Wang Y




Has Abstract


2018-01-01 00:00:00












  • Gene expression alterations in formalin-fixed, paraffin-embedded Barrett esophagus and esophageal adenocarcinoma tissues.

    abstract:BACKGROUND AND AIM:Widespread applicability of tissue-based mRNA expression screening for Barrett esophagus (BE) is likely to require (1) accurate methods for assaying archival formalin-fixed, paraffin-embedded (FFPE) histopathology specimens taken at endoscopy, and (2) validation studies of promising biomarkers in dif...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Botelho NK,Schneiders FI,Lord SJ,Freeman AK,Tyagi S,Nancarrow DJ,Hayward NK,Whiteman DC,Lord RV

    更新日期:2010-07-15 00:00:00

  • Autophagy limits the cytotoxic effects of the AKT inhibitor AZ7328 in human bladder cancer cells.

    abstract:BACKGROUND:Mutations that activate the PI3K/AKT/mTOR pathway are relatively common in urothelial (bladder) cancers, but how these pathway mutations affect AKT dependency is not known. We characterized the relationship between AKT pathway mutational status and sensitivity to the effects of the selective AKT kinase inhib...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Dickstein RJ,Nitti G,Dinney CP,Davies BR,Kamat AM,McConkey DJ

    更新日期:2012-11-01 00:00:00

  • Bruton's tyrosine kinase is a potential therapeutic target in prostate cancer.

    abstract::Bruton's tyrosine kinase (BTK) is a non-receptor tyrosine kinase that has mainly been studied in haematopoietic cells. We have investigated whether BTK is a potential therapeutic target in prostate cancer. We find that BTK is expressed in prostate cells, with the alternate BTK-C isoform predominantly expressed in pros...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Kokabee L,Wang X,Sevinsky CJ,Wang WL,Cheu L,Chittur SV,Karimipoor M,Tenniswood M,Conklin DS

    更新日期:2015-01-01 00:00:00

  • Cell adhesion molecules as targets for therapy of neuroblastoma.

    abstract::Cell adhesion molecules (CAMs) are glycoproteins expressed on the surface of cell membranes. In normal cells, CAMs participate in a variety of biological processes including cell proliferation, migration and differentiation. In tumor cells, CAMs have been reported to function as oncogenes or tumor suppressors, in sign...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Yoon KJ,Danks MK

    更新日期:2009-02-01 00:00:00

  • The genetics of FANCC and FANCG in familial pancreatic cancer.

    abstract::Patients with Fanconi anemia (FA) display a wide variety of defects including bone marrow failure and a high risk of developing cancer. Multiple Fanconi genes exist whose proteins form a complex that along with BRCA1 is important for the translocalization of FANCD2 to nuclear foci. With BRCA2 and RAD51, this complex i...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Rogers CD,van der Heijden MS,Brune K,Yeo CJ,Hruban RH,Kern SE,Goggins M

    更新日期:2004-02-01 00:00:00

  • Randomized discontinuation trial of sorafenib (BAY 43-9006).

    abstract::With the increasing interest in development of cytostatic anticancer drugs, the randomized discontinuation trial (RDT) design has been proved to be useful in the evaluation of their clinical activity. In the June 1, 2006 issue of the Journal of Clinical Oncology, a study by Ratain et al. uses RDT in a phase-II placebo...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,随机对照试验


    authors: Jain L,Venitz J,Figg WD

    更新日期:2006-10-01 00:00:00

  • Stromal-epithelial interactions are responsible for prostate tumor progression through an androgen-related mechanism.

    abstract::While several hypotheses have been put forward to explain how prostate tumors become resistant to androgen deprivation therapy, the mechanism by which prostate tumors have increased androgen concentrations as compared to the serum has been poorly explored. Using a stromal/epithelial cell co-culture model, Mizokami et ...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Sharma H,Sissung TM,Pressler H,Figg WD

    更新日期:2010-02-01 00:00:00

  • Genetic polymorphisms in OGG1 and their association with angiomyolipoma, a benign kidney tumor in patients with tuberous sclerosis.

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    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Habib SL,Danial E,Nath S,Schneider J,Jenkinson CP,Duggirala R,Abboud HE,Thameem F

    更新日期:2008-01-01 00:00:00

  • Regulation of Myc function by ARF: checkpoint for Myc-induced oncogenesis.

    abstract::The alterative reading frame (ARF) protein is unique in its capacity to interact with Mdm2 thus facilitating p53-dependent cell cycle arrest and apoptosis. ARF also acts in a p53-independent manner in which it binds to Myc and interferes with transcriptional activation by Myc thereby inhibiting Myc-induced cell prolif...

    journal_title:Cancer biology & therapy

    pub_type: 评论,杂志文章


    authors: Sarkar D,Fisher PB

    更新日期:2006-06-01 00:00:00

  • Photodynamic therapy activated signaling from epidermal growth factor receptor and STAT3: Targeting survival pathways to increase PDT efficacy in ovarian and lung cancer.

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    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Edmonds C,Hagan S,Gallagher-Colombo SM,Busch TM,Cengel KA

    更新日期:2012-12-01 00:00:00

  • Human prostate cancer ZIP1/zinc/citrate genetic/metabolic relationship in the TRAMP prostate cancer animal model.

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    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Costello LC,Franklin RB,Zou J,Feng P,Bok R,Swanson MG,Kurhanewicz J

    更新日期:2011-12-15 00:00:00

  • Multiplying therapies and reducing toxicity in metastatic melanoma.

    abstract::Prior to 2011, only 2 systemic treatments were approved for the treatment of melanoma and these had limited efficacy. In the past 4 years, 6 novel agents have received FDA approval. Herein, we will focus on 4 recently published NEJM papers reporting the results of clinical trials, comprising 4 agents targeting the MAP...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Massey PR,Prasad V,Figg WD,Fojo T

    更新日期:2015-01-01 00:00:00

  • Acquired resistance of lung adenocarcinoma to EGFR-tyrosine kinase inhibitors gefitinib and erlotinib.

    abstract::Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs), such as gefitinib and erlotinib, are the first targeted therapy drugs approved for the treatment of advanced non-small-cell lung cancer (NSCLC). Interestingly, treatment with these small molecule, reversible EGFR-TKIs leads to more positive respo...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Xu Y,Liu H,Chen J,Zhou Q

    更新日期:2010-04-15 00:00:00

  • Rapamycin enhances cetuximab cytotoxicity by inhibiting mTOR-mediated drug resistance in mesenchymal hepatoma cells.

    abstract::The synergistic effect of combined drug therapy provides an enhanced treatment for advanced liver cancer. We aimed to investigate the underlying mechanism of cetuximab sensitization by rapamycin in hepatoma cells. Four hepatoma cell lines, HepG2, HuH7, SNU-387, and SNU-449, were treated with cetuximab or cetuximab plu...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Chen W,Hu QD,Xia XF,Liang C,Liu H,Zhang Q,Ma T,Liang F,Liang TB

    更新日期:2014-08-01 00:00:00

  • Targeting hypoxia and angiogenesis through HIF-1alpha inhibition.

    abstract::Hypoxia is an important phenomenon in the tumor microenvironment. Hypoxic tumors are more aggressive and resistant to anti-neoplastic treatments. HIF-1alpha plays a major role in the response of tumors to hypoxia, and it is mainly responsible for the "angiogenic switch". HIF-1alpha contributes to tumor aggressiveness,...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Diaz-Gonzalez JA,Russell J,Rouzaut A,Gil-Bazo I,Montuenga L

    更新日期:2005-10-01 00:00:00

  • Doxorubicin and 5-fluorouracil induced accumulation and transcriptional activity of p53 are independent of the phosphorylation at serine 15 in MCF-7 breast cancer cells.

    abstract::The chemotherapeutic agents doxorubicin (dox) or 5-fluorouracil (5FU) are used to treat cancer cells as they cause irreparable DNA damage, inducing these aberrant cells to undergo cell death. The mediator of this process is presumed to be in part the tumor suppressor p53 which regulates genes involved in DNA repair an...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Balmer MT,Katz RD,Liao S,Goodwine JS,Gal S

    更新日期:2014-08-01 00:00:00

  • Complete and sustained response of adult medulloblastoma to first-line sonic hedgehog inhibition with vismodegib.

    abstract::Medulloblastoma is an aggressive primitive neuroectodermal tumor of the cerebellum that is rare in adults. Medulloblastomas fall into 4 prognostically significant molecular subgroups that are best defined by experimental gene expression profiles: the WNT pathway, sonic hedgehog (SHH) pathway, and subgroups 3 and 4 (no...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Lou E,Schomaker M,Wilson JD,Ahrens M,Dolan M,Nelson AC

    更新日期:2016-10-02 00:00:00

  • Significant response to apatinib monotherapy in heavily pretreated advanced HER2-positive breast cancer: a case report and literature review.

    abstract::Although HER2-targeted therapy has been shown to prolong the survival of patients with HER2-positive breast cancer, most patients eventually progress due to drug resistance. Novel treatment options are urgently needed to overcome resistance to HER2-targeted therapy. The VEGF/VEGFR (Vascular endothelial growth factor a...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Danni L,Lingyun Z,Jian W,Hongfei Y,Lu X,Peng Y,Xiujuan Q,Yunpeng L,Yuee T

    更新日期:2020-07-02 00:00:00

  • ETS-TMPRSS2 fusion gene products in prostate cancer.

    abstract::Genes playing a role in carcinogenesis have often been identified through analysis of recurrent chromosomal rearrangements. Although such rearrangements are well known in leukemias, lymphomas, and sarcomas, they have not been well characterized in carcinomas. In the October 28, 2005 issue of Science, a study by Tomlin...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Ahlers CM,Figg WD

    更新日期:2006-03-01 00:00:00

  • Characterization of rare transforming KRAS mutations in sporadic colorectal cancer.

    abstract::KRAS mutational status has been shown to be a predictive biomarker of resistance to anti-EGFR monoclonal antibody (mAb) therapy in patients with metastatic colorectal cancer. We report the spectrum of KRAS mutation in 1506 patients with colorectal cancer and the identification and characterization of rare insertion mu...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Tong JH,Lung RW,Sin FM,Law PP,Kang W,Chan AW,Ma BB,Mak TW,Ng SS,To KF

    更新日期:2014-06-01 00:00:00

  • Glycogen synthase kinase-3 beta inhibitors as novel cancer treatments and modulators of antitumor immune responses.

    abstract::As a kinase at the crossroads of numerous metabolic and cell growth signaling pathways, glycogen synthase kinase-3 beta (GSK-3β) is a highly desirable therapeutic target in cancer. Despite its involvement in pathways associated with the pathogenesis of several malignancies, no selective GSK-3β inhibitor has been appro...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章,评审


    authors: Sahin I,Eturi A,De Souza A,Pamarthy S,Tavora F,Giles FJ,Carneiro BA

    更新日期:2019-01-01 00:00:00

  • Remarkable enhancement of cytotoxicity of onconase and cepharanthine when used in combination on various tumor cell lines.

    abstract::Onconase (Onc), a ribonuclease from oocytes or early embryos of Northern Leopard frog (Rana pipiens), is cytostatic and cytotoxic to a variety of tumor lines in vitro, inhibits growth of tumors in animal in vivo models and is currently in Phase IIIb clinical trials for malignant mesothelioma where it displays antitumo...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Ita M,Halicka HD,Tanaka T,Kurose A,Ardelt B,Shogen K,Darzynkiewicz Z

    更新日期:2008-07-01 00:00:00

  • Kmt2a cooperates with menin to suppress tumorigenesis in mouse pancreatic islets.

    abstract::The reported incidence of pancreatic neuroendocrine tumors (PanNETs) has increased, due in large part to improvements in detection and awareness. However, therapeutic options are limited and a critical need exists for understanding a more thorough characterization of the molecular pathology underlying this disease. Th...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Lin W,Francis JM,Li H,Gao X,Pedamallu CS,Ernst P,Meyerson M

    更新日期:2016-12-01 00:00:00

  • The PTEN/PI3K/Akt pathway regulates stem-like cells in primary esophageal carcinoma cells.

    abstract::Recent reports have shown that cancer stem cells exist in many malignancies. Side population (SP) cells are used to enrich cancer stem-like cells in many cell lines and fresh tumor specimens. In this study, we cultured primary esophageal squamous cell carcinoma (ESCC) cells from ESCC tissue specimens. SP cells from pr...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Li H,Gao Q,Guo L,Lu SH

    更新日期:2011-06-01 00:00:00

  • Anticancer imidazoacridinone C-1311 inhibits hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and angiogenesis.

    abstract::Antitumor imidazoacridinone C-1311 is a DNA-reactive topoisomerase II and FLT3 receptor tyrosine kinase inhibitor. Here, we demonstrate the mechanism of C-1311 inhibitory action on novel targets: hypoxia-inducible factor-1α (HIF-1α), vascular-endothelial growth factor (VEGF), and angiogenesis. In a cell-free system, C...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Paradziej-Łukowicz J,Skwarska A,Peszyńska-Sularz G,Brillowska-Dąbrowska A,Konopa J

    更新日期:2011-10-01 00:00:00

  • Annexin-1 downregulation in thyroid cancer correlates to the degree of tumor differentiation.

    abstract::We investigated the expression of annexin-1 (ANXA1) in thyroid carcinoma cell lines and in thyroid cancers with a different degree of differentiation. The highest level of ANXA1 expression examined by Western blotting was detected in the papillary carcinoma cells (NPA) and in the follicular cells (WRO). On the other h...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Petrella A,Festa M,Ercolino SF,Zerilli M,Stassi G,Solito E,Parente L

    更新日期:2006-06-01 00:00:00

  • Histone deacetylase inhibitors modulate renal cell carcinoma sensitivity to TRAIL/Apo-2L-induced apoptosis by enhancing TRAIL-R2 expression.

    abstract::Every year, 12,000 people in the U.S. die from renal cell carcinoma. Current therapies include partial or complete nephrectomy or treatments such as administration of IFN-alpha and/or interleukins that are moderately effective, at best. Moreover, the current therapies are invasive and inefficient and new therapies are...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: VanOosten RL,Moore JM,Karacay B,Griffith TS

    更新日期:2005-10-01 00:00:00

  • KLF4 inhibition of lung cancer cell invasion by suppression of SPARC expression.

    abstract::Krüppel-Like Factor 4 (KLF4) functions as a tumor suppressor in some cancers, but its molecular mechanism is not clear. Our recent study also showed that the expression of KLF4 is dramatically reduced in primary lung cancer tissues. To investigate the possible role of KLF4 in lung cancer, we stably transfected KLF4 in...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Zhou Y,Hofstetter WL,He Y,Hu W,Pataer A,Wang L,Wang J,Zhou Y,Yu L,Fang B,Swisher SG

    更新日期:2010-04-01 00:00:00

  • Furanonaphthoquinones cause apoptosis of cancer cells by inducing the production of reactive oxygen species by the mitochondrial voltage-dependent anion channel.

    abstract::The mitochondrial production of reactive oxygen species has been implicated in the anticancer activity of furanonaphthoquinone. However, the mechanism of the activation remains elusive. In the current study, we found that treatment of HeLa cells with 2-methyl-5(or -8)-hydroxy-furanonaphthoquinone (FNQ13) induces mitoc...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Simamura E,Hirai K,Shimada H,Koyama J,Niwa Y,Shimizu S

    更新日期:2006-11-01 00:00:00

  • Phase II trial of single agent Val-boroPro (Talabostat) inhibiting Fibroblast Activation Protein in patients with metastatic colorectal cancer.

    abstract:PURPOSE:Fibroblast Activation Protein (FAP) is a tumor fibroblast protease that has been shown to potentiate colorectal cancer growth. The clinical impact of FAP inhibition was tested using Val-boroPro (Talabostat), the first clinical inhibitor of FAP enzymatic activity, in a phase II study of patients with metastatic ...

    journal_title:Cancer biology & therapy

    pub_type: 杂志文章


    authors: Narra K,Mullins SR,Lee HO,Strzemkowski-Brun B,Magalong K,Christiansen VJ,McKee PA,Egleston B,Cohen SJ,Weiner LM,Meropol NJ,Cheng JD

    更新日期:2007-11-01 00:00:00