Kinetoplast DNA minicircles: regions of extensive sequence divergence.

Abstract:

:Previous work has shown that the kinetoplast minicircle DNA of Leishmania species exhibits species-specific sequence divergence and this observation has led to the development of a DNA probe-based diagnostic test for leishmaniasis. In the work reported here, we demonstrate that the minicircle is composed of three types of DNA sequences with differing specificities reflecting different rates of DNA sequence change. A library of cloned fragments of kinetoplast DNA (kDNA) from Leishmania mexicana amazonensis was prepared and the cloned subfragments were found to contain DNA sequences with different taxonomic specificities based on hybridization analysis with various species of Leishmania. Four groups of subfragments were found, those that hybridized with a large number of Leishmania sp. as well as sequences unique to the species, subspecies, or isolate. Analysis of nested deletions of a single, full-length minicircle demonstrates that these different taxonomic specificities are contained within a single minicircle. This implies that different regions of a single minicircle have DNA sequences that diverge at different rates. These sequences represent potentially valuable tools in diagnostic, epidemiologic, and ecological studies of leishmaniasis and provide the basis for a model of kDNA sequence evolution.

authors

Rogers WO,Wirth DF

doi

10.1073/pnas.84.2.565

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

565-9

issue

2

eissn

0027-8424

issn

1091-6490

journal_volume

84

pub_type

杂志文章
  • The beta 1 domain of the mouse E beta chain is important for restricted antigen presentation to helper T-cell hybridomas.

    abstract::We have constructed a hybrid E beta gene by replacing the second exon of the Ed beta gene (which encodes the majority of the beta 1 protein domain) with the corresponding exon from the Eb beta gene. The hybrid gene has been introduced into a d haplotype host, the lymphoma beta-cell line A20-2J, and an E alpha E beta d...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.6.1678

    authors: Folsom V,Gay D,Tonegawa S

    更新日期:1985-03-01 00:00:00

  • Thiazide diuretic drug receptors in rat kidney: identification with [3H]metolazone.

    abstract::Thiazides and related diuretics inhibit NaCl reabsorption in the distal tubule through an unknown mechanism. We report here that [3H]metolazone, a diuretic with a thiazide-like mechanism of action, labels a site in rat kidney membranes that has characteristics of the thiazide-sensitive ion transporter. [3H]Metolazone ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.7.2311

    authors: Beaumont K,Vaughn DA,Fanestil DD

    更新日期:1988-04-01 00:00:00

  • Epigenetic silencing of a Ca(2+)-regulated Ras GTPase-activating protein RASAL defines a new mechanism of Ras activation in human cancers.

    abstract::Ras has achieved notoriety as an oncogene aberrantly activated in multiple human tumors. Approximately 30% of all human tumors express an oncogenic form of this GTPase that is locked in an active conformation as a result of being insensitive to Ras GTPase-activating proteins (GAPs), proteins that normally regulate the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0700153104

    authors: Jin H,Wang X,Ying J,Wong AH,Cui Y,Srivastava G,Shen ZY,Li EM,Zhang Q,Jin J,Kupzig S,Chan AT,Cullen PJ,Tao Q

    更新日期:2007-07-24 00:00:00

  • Colloquium paper: terrestrial apes and phylogenetic trees.

    abstract::The image that best expresses Darwin's thinking is the tree of life. However, Darwin's human evolutionary tree lacked almost everything because only the Neanderthals were known at the time and they were considered one extreme expression of our own species. Darwin believed that the root of the human tree was very deep ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0914614107

    authors: Arsuaga JL

    更新日期:2010-05-11 00:00:00

  • Hyper telomere recombination accelerates replicative senescence and may promote premature aging.

    abstract::Werner syndrome and Bloom syndrome result from defects in the RecQ helicases Werner (WRN) and Bloom (BLM), respectively, and display premature aging phenotypes. Similarly, XFE progeroid syndrome results from defects in the ERCC1-XPF DNA repair endonuclease. To gain insight into the origin of cellular senescence and hu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1006338107

    authors: Hagelstrom RT,Blagoev KB,Niedernhofer LJ,Goodwin EH,Bailey SM

    更新日期:2010-09-07 00:00:00

  • Phosphoglucose isomerase: a ketol isomerase with aldol C2-epimerase activity.

    abstract::With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). The identity of M6P and its formation from G6P plus F6P a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.4.1237

    authors: Seeholzer SH

    更新日期:1993-02-15 00:00:00

  • Spontaneous wettability patterning via creasing instability.

    abstract::Surfaces with patterned wettability contrast are important in industrial applications such as heat transfer, water collection, and particle separation. Traditional methods of fabricating such surfaces rely on microfabrication technologies, which are only applicable to certain substrates and are difficult to scale up a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1522700113

    authors: Chen D,McKinley GH,Cohen RE

    更新日期:2016-07-19 00:00:00

  • Nucleosomes associated with newly replicated DNA have an altered conformation.

    abstract::In vitro DNA synthesis was studied in HeLa cell nuclei, with emphasis on the question of whether newly replicated DNA is associated with nucleosomes. The newly replicated DNA was twice as sensitive to digestion by micrococcal nuclease as mature chromatin DNA, reaching a limit digest at 20-25% acid-insoluble product. E...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.75.6.2717

    authors: Seale RL

    更新日期:1978-06-01 00:00:00

  • Homology-independent discovery of replicating pathogenic circular RNAs by deep sequencing and a new computational algorithm.

    abstract::A common challenge in pathogen discovery by deep sequencing approaches is to recognize viral or subviral pathogens in samples of diseased tissue that share no significant homology with a known pathogen. Here we report a homology-independent approach for discovering viroids, a distinct class of free circular RNA subvir...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1117815109

    authors: Wu Q,Wang Y,Cao M,Pantaleo V,Burgyan J,Li WX,Ding SW

    更新日期:2012-03-06 00:00:00

  • Developmental topography of cortical thickness during infancy.

    abstract::During the first 2 postnatal years, cortical thickness of the human brain develops dynamically and spatially heterogeneously and likely peaks between 1 and 2 y of age. The striking development renders this period critical for later cognitive outcomes and vulnerable to early neurodevelopmental disorders. However, due t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1821523116

    authors: Wang F,Lian C,Wu Z,Zhang H,Li T,Meng Y,Wang L,Lin W,Shen D,Li G

    更新日期:2019-08-06 00:00:00

  • Recognition of triple-helical DNA structures by transposon Tn7.

    abstract::We have found that the bacterial transposon Tn7 can recognize and preferentially insert adjacent to triple-helical nucleic acid structures. Both synthetic intermolecular triplexes, formed through the pairing of a short triplex-forming oligonucleotide on a plasmid DNA, and naturally occurring mirror repeat sequences kn...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.080061497

    authors: Rao JE,Miller PS,Craig NL

    更新日期:2000-04-11 00:00:00

  • Host and invader impact of transfer of the clc genomic island into Pseudomonas aeruginosa PAO1.

    abstract::Genomic islands, large potentially mobile regions of bacterial chromosomes, are a major contributor to bacteria evolution. Here, we investigated the fitness cost and phenotypic differences between the bacterium Pseudomonas aeruginosa PAO1 and a derivative carrying one integrated copy of the clc element, a 103-kb genom...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0801269105

    authors: Gaillard M,Pernet N,Vogne C,Hagenbüchle O,van der Meer JR

    更新日期:2008-05-13 00:00:00

  • Spatial and morphological reorganization of endosymbiosis during metamorphosis accommodates adult metabolic requirements in a weevil.

    abstract::Bacterial intracellular symbiosis (endosymbiosis) is widespread in nature and impacts many biological processes. In holometabolous symbiotic insects, metamorphosis entails a complete and abrupt internal reorganization that creates a constraint for endosymbiont transmission from larvae to adults. To assess how endosymb...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2007151117

    authors: Maire J,Parisot N,Galvao Ferrarini M,Vallier A,Gillet B,Hughes S,Balmand S,Vincent-Monégat C,Zaidman-Rémy A,Heddi A

    更新日期:2020-08-11 00:00:00

  • Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor alpha.

    abstract::The biological roles of estrogen-responsive finger protein (efp) in vivo were evaluated in mice carrying a loss-of-function mutation in efp by gene-targeted mutagenesis. Although efp homozygous mice were viable and fertile in both sexes, the uterus that expressed abundant estrogen receptor alpha exhibited significant ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.21.12027

    authors: Orimo A,Inoue S,Minowa O,Tominaga N,Tomioka Y,Sato M,Kuno J,Hiroi H,Shimizu Y,Suzuki M,Noda T,Muramatsu M

    更新日期:1999-10-12 00:00:00

  • Transcriptome profiling reveals mosaic genomic origins of modern cultivated barley.

    abstract::The domestication of cultivated barley has been used as a model system for studying the origins and early spread of agrarian culture. Our previous results indicated that the Tibetan Plateau and its vicinity is one of the centers of domestication of cultivated barley. Here we reveal multiple origins of domesticated bar...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1414335111

    authors: Dai F,Chen ZH,Wang X,Li Z,Jin G,Wu D,Cai S,Wang N,Wu F,Nevo E,Zhang G

    更新日期:2014-09-16 00:00:00

  • Isolation of viable Hodgkin and Reed-Sternberg cells from Hodgkin disease tissues.

    abstract::Hodgkin disease (HD) is characterized by a small number of malignant Hodgkin and Reed-Sternberg (H/RS) cells among a major population of nonmalignant cells. The analysis of H/RS cells has been hampered by their low frequency and fragility. Here, we describe the isolation of viable H/RS cells from HD affected tissues b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.17.10117

    authors: Irsch J,Nitsch S,Hansmann ML,Rajewsky K,Tesch H,Diehl V,Jox A,Küppers R,Radbruch A

    更新日期:1998-08-18 00:00:00

  • The importance of the Montreal Protocol in protecting climate.

    abstract::The 1987 Montreal Protocol on Substances that Deplete the Ozone Layer is a landmark agreement that has successfully reduced the global production, consumption, and emissions of ozone-depleting substances (ODSs). ODSs are also greenhouse gases that contribute to the radiative forcing of climate change. Using historical...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0610328104

    authors: Velders GJ,Andersen SO,Daniel JS,Fahey DW,McFarland M

    更新日期:2007-03-20 00:00:00

  • Interchain disulfide bonds in procollagen are located in a large nontriple-helical COOH-terminal domain.

    abstract::Tadpole collagenase (EC 3.4.24.3) cleaved chick cranial bone procollagen into two triple-stranded fragments, PCA and PCB. Only PCB, with an estimated molecular weight of about 60,000 for each component chain after reduction, was found to contain interchain disulfide bonds. The analogous cleavage of collagen is known t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.8.3009

    authors: Byers PH,Click EM,Harper E,Bornstein P

    更新日期:1975-08-01 00:00:00

  • Stimulation of human platelet guanylate cyclase by unsaturated fatty acid peroxides.

    abstract::Guanylate cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] activity of human platelet homogenates was stimulated by the addition of phospholipase A2 or unsaturated fatty acids such as oleic, vaccenic, linoleic, linolenic, eicosenoic, eicosadienoic, and arachidonic acids. The addition of lipoxidase potentiated...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.9.3657

    authors: Hidaka H,Asano T

    更新日期:1977-09-01 00:00:00

  • Contribution of phenotypic heterogeneity to adaptive antibiotic resistance.

    abstract::Antibiotic-resistant isolates of Salmonella enterica were selected on plates containing lethal concentrations of rifampicin, kanamycin, and nalidixic acid. The stability of the resistance phenotype was scored after nonselective growth. Rifampicin-resistant (Rif(r)) isolates were stable, suggesting that they had arisen...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1316084111

    authors: Sánchez-Romero MA,Casadesús J

    更新日期:2014-01-07 00:00:00

  • 2',5'-Bis-O-(tert-butyldimethylsilyl)-3'-spiro-5''-(4''-amino-1'',2''- oxathiole-2'',2'-dioxide)pyrimidine (TSAO) nucleoside analogues: highlyselective inhibitors of human immunodeficiency virus type 1 that are targeted at the viral reverse transcriptase.

    abstract::A series of pyrimidine nucleoside analogues containing [2',5'-bis-O-(tert-butyldimethylsilyl)-3'-spiro-5''-(4''-amino- 1'',2''-oxathiole-2'',2''-dioxide)]-beta-D-ribofuranose as the pentose were found to inhibit human immunodeficiency virus type 1 [HIV-1(IIIB)] replication at a concentration of 0.06-0.8 microM but wer...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.10.4392

    authors: Balzarini J,Pérez-Pérez MJ,San-Félix A,Schols D,Perno CF,Vandamme AM,Camarasa MJ,De Clercq E

    更新日期:1992-05-15 00:00:00

  • Chronic sleep curtailment, even without extended (>16-h) wakefulness, degrades human vigilance performance.

    abstract::Millions of individuals routinely remain awake for more than 18 h daily, which causes performance decrements. It is unknown if these functional impairments are the result of that extended wakefulness or from the associated shortened sleep durations. We therefore examined changes in objective reaction time performance ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1706694115

    authors: McHill AW,Hull JT,Wang W,Czeisler CA,Klerman EB

    更新日期:2018-06-05 00:00:00

  • Schrödinger equation revisited.

    abstract::The time-dependent Schrödinger equation is a cornerstone of quantum physics and governs all phenomena of the microscopic world. However, despite its importance, its origin is still not widely appreciated and properly understood. We obtain the Schrödinger equation from a mathematical identity by a slight generalization...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1302475110

    authors: Schleich WP,Greenberger DM,Kobe DH,Scully MO

    更新日期:2013-04-02 00:00:00

  • Identification and primary sequence of an unspliced human urokinase poly(A)+ RNA.

    abstract::Human urokinase cDNA clones have been identified from a cDNA library prepared from total RNA of human fibroblasts transformed by simian virus 40 [Okayama, H. & Berg, P. (1983) Mol. Cell. Biol. 3, 280-289]. Synthetic oligonucleotides, corresponding to urokinase protein sequence, were used as probes. The cloned cDNA cov...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.15.4727

    authors: Verde P,Stoppelli MP,Galeffi P,Di Nocera P,Blasi F

    更新日期:1984-08-01 00:00:00

  • Microbial iron transport via a siderophore shuttle: a membrane ion transport paradigm.

    abstract::A mechanism of ion transport across membranes is reported. Microbial transport of Fe(3+) generally delivers iron, a growth-limiting nutrient, to cells via highly specific siderophore-mediated transport systems. In contrast, iron transport in the fresh water bacterium Aeromonas hydrophila is found to occur by means of ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.200318797

    authors: Stintzi A,Barnes C,Xu J,Raymond KN

    更新日期:2000-09-26 00:00:00

  • Targeted ablation of cholinergic interneurons in the dorsolateral striatum produces behavioral manifestations of Tourette syndrome.

    abstract::Gilles de la Tourette syndrome (TS) is characterized by tics, which are transiently worsened by stress, acute administration of dopaminergic drugs, and by subtle deficits in motor coordination and sensorimotor gating. It represents the most severe end of a spectrum of tic disorders that, in aggregate, affect ∼ 5% of t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1419533112

    authors: Xu M,Kobets A,Du JC,Lennington J,Li L,Banasr M,Duman RS,Vaccarino FM,DiLeone RJ,Pittenger C

    更新日期:2015-01-20 00:00:00

  • Isoflurane inhibits synaptic vesicle exocytosis through reduced Ca2+ influx, not Ca2+-exocytosis coupling.

    abstract::Identifying presynaptic mechanisms of general anesthetics is critical to understanding their effects on synaptic transmission. We show that the volatile anesthetic isoflurane inhibits synaptic vesicle (SV) exocytosis at nerve terminals in dissociated rat hippocampal neurons through inhibition of presynaptic Ca(2+) inf...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1500525112

    authors: Baumgart JP,Zhou ZY,Hara M,Cook DC,Hoppa MB,Ryan TA,Hemmings HC Jr

    更新日期:2015-09-22 00:00:00

  • Sonic Hedgehog promotes the development of multipotent neural crest progenitors endowed with both mesenchymal and neural potentials.

    abstract::In the vertebrate embryo, the cephalic neural crest cells (CNCCs) produce cells belonging to two main lineages: the neural [including neurons, glial cells of the peripheral nervous system (PNS), and melanocytes] and the mesenchymal (chondrocytes, osteoblasts, smooth muscle cells, and connective tissue cells), whereas ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0708806104

    authors: Calloni GW,Glavieux-Pardanaud C,Le Douarin NM,Dupin E

    更新日期:2007-12-11 00:00:00

  • Processing of the mother-cell sigma factor, sigma K, may depend on events occurring in the forespore during Bacillus subtilis development.

    abstract::During sporulation of the Gram-positive bacterium Bacillus subtilis, transcription of genes encoding spore coat proteins in the mother-cell compartment of the sporangium is controlled by RNA polymerase containing the sigma subunit called sigma K. Based on comparison of the N-terminal amino acid sequence of sigma K wit...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.24.9722

    authors: Lu S,Halberg R,Kroos L

    更新日期:1990-12-01 00:00:00

  • The misleading narrative of the canonical faculty productivity trajectory.

    abstract::A scientist may publish tens or hundreds of papers over a career, but these contributions are not evenly spaced in time. Sixty years of studies on career productivity patterns in a variety of fields suggest an intuitive and universal pattern: Productivity tends to rise rapidly to an early peak and then gradually decli...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1702121114

    authors: Way SF,Morgan AC,Clauset A,Larremore DB

    更新日期:2017-10-31 00:00:00