A natural variant and engineered mutation in a GPCR promote DEET resistance in C. elegans.


:DEET (N,N-diethyl-meta-toluamide) is a synthetic chemical identified by the US Department of Agriculture in 1946 in a screen for repellents to protect soldiers from mosquito-borne diseases1,2. Since its discovery, DEET has become the world's most widely used arthropod repellent and is effective against invertebrates separated by millions of years of evolution-including biting flies3, honeybees4, ticks5, and land leeches3. In insects, DEET acts on the olfactory system5-12 and requires the olfactory receptor co-receptor Orco7,9-12, but exactly how it works remains controversial13. Here we show that the nematode Caenorhabditis elegans is sensitive to DEET and use this genetically tractable animal to study the mechanism of action of this chemical. We found that DEET is not a volatile repellent, but instead interferes selectively with chemotaxis to a variety of attractant and repellent molecules. In a forward genetic screen for DEET-resistant worms, we identified a gene that encodes a single G protein-coupled receptor, str-217, which is expressed in a single pair of chemosensory neurons that are responsive to DEET, called ADL neurons. Mis-expression of str-217 in another chemosensory neuron conferred responses to DEET. Engineered str-217 mutants, and a wild isolate of C. elegans that carries a str-217 deletion, are resistant to DEET. We found that DEET can interfere with behaviour by inducing an increase in average pause length during locomotion, and show that this increase in pausing requires both str-217 and ADL neurons. Finally, we demonstrated that ADL neurons are activated by DEET and that optogenetic activation of ADL neurons increased average pause length. This is consistent with the 'confusant' hypothesis, which proposes that DEET is not a simple repellent but that it instead modulates multiple olfactory pathways to scramble behavioural responses10,11. Our results suggest a consistent motif in the effectiveness of DEET across widely divergent taxa: an effect on multiple chemosensory neurons that disrupts the pairing between odorant stimulus and behavioural response.






Dennis EJ,Dobosiewicz M,Jin X,Duvall LB,Hartman PS,Bargmann CI,Vosshall LB




Has Abstract


2018-10-01 00:00:00
















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    pub_type: 杂志文章


    authors: Cutler A,Mehler J,Norris D,Segui J

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  • The digital code of DNA.

    abstract::The discovery of the structure of DNA transformed biology profoundly, catalysing the sequencing of the human genome and engendering a new view of biology as an information science. Two features of DNA structure account for much of its remarkable impact on science: its digital nature and its complementarity, whereby on...


    pub_type: 评论,杂志文章,评审


    authors: Hood L,Galas D

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  • Extreme hydrothermal conditions at an active plate-bounding fault.

    abstract::Temperature and fluid pressure conditions control rock deformation and mineralization on geological faults, and hence the distribution of earthquakes. Typical intraplate continental crust has hydrostatic fluid pressure and a near-surface thermal gradient of 31 ± 15 degrees Celsius per kilometre. At temperatures above ...


    pub_type: 杂志文章


    authors: Sutherland R,Townend J,Toy V,Upton P,Coussens J,Allen M,Baratin LM,Barth N,Becroft L,Boese C,Boles A,Boulton C,Broderick NGR,Janku-Capova L,Carpenter BM,Célérier B,Chamberlain C,Cooper A,Coutts A,Cox S,Craw L,Do

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  • Interneuron cell types are fit to function.

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    pub_type: 杂志文章,评审


    authors: Kepecs A,Fishell G

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    pub_type: 杂志文章


    authors: Fonseca-Pereira D,Arroz-Madeira S,Rodrigues-Campos M,Barbosa IA,Domingues RG,Bento T,Almeida AR,Ribeiro H,Potocnik AJ,Enomoto H,Veiga-Fernandes H

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    authors: Grivennikov SI,Wang K,Mucida D,Stewart CA,Schnabl B,Jauch D,Taniguchi K,Yu GY,Osterreicher CH,Hung KE,Datz C,Feng Y,Fearon ER,Oukka M,Tessarollo L,Coppola V,Yarovinsky F,Cheroutre H,Eckmann L,Trinchieri G,Karin M

    更新日期:2012-11-08 00:00:00

  • Suppressor effects and cyclic AMP accumulation by the CD29 molecule of CD4+ lymphocytes.

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    pub_type: 杂志文章


    authors: Groux H,Huet S,Valentin H,Pham D,Bernard A

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  • The community of the self.

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    authors: Raff MC,Miller RH,Noble M

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    authors: Spencer DT,Drake T,Briles TC,Stone J,Sinclair LC,Fredrick C,Li Q,Westly D,Ilic BR,Bluestone A,Volet N,Komljenovic T,Chang L,Lee SH,Oh DY,Suh MG,Yang KY,Pfeiffer MHP,Kippenberg TJ,Norberg E,Theogarajan L,Vahala K

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    authors: Nguyen NUN,Canseco DC,Xiao F,Nakada Y,Li S,Lam NT,Muralidhar SA,Savla JJ,Hill JA,Le V,Zidan KA,El-Feky HW,Wang Z,Ahmed MS,Hubbi ME,Menendez-Montes I,Moon J,Ali SR,Le V,Villalobos E,Mohamed MS,Elhelaly WM,Thet

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    authors: Gearing AJ,Beckett P,Christodoulou M,Churchill M,Clements J,Davidson AH,Drummond AH,Galloway WA,Gilbert R,Gordon JL

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  • Enamel proteome shows that Gigantopithecus was an early diverging pongine.

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    authors: Welker F,Ramos-Madrigal J,Kuhlwilm M,Liao W,Gutenbrunner P,de Manuel M,Samodova D,Mackie M,Allentoft ME,Bacon AM,Collins MJ,Cox J,Lalueza-Fox C,Olsen JV,Demeter F,Wang W,Marques-Bonet T,Cappellini E

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  • Neural circuitry linking mating and egg laying in Drosophila females.

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    authors: Nik-Zainal S,Davies H,Staaf J,Ramakrishna M,Glodzik D,Zou X,Martincorena I,Alexandrov LB,Martin S,Wedge DC,Van Loo P,Ju YS,Smid M,Brinkman AB,Morganella S,Aure MR,Lingjærde OC,Langerød A,Ringnér M,Ahn SM,Boyault S

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    authors: Chomiuk L,Linford JD,Yang J,O'Brien TJ,Paragi Z,Mioduszewski AJ,Beswick RJ,Cheung CC,Mukai K,Nelson T,Ribeiro VA,Rupen MP,Sokoloski JL,Weston J,Zheng Y,Bode MF,Eyres S,Roy N,Taylor GB

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    authors: Goodarzi H,Zhang S,Buss CG,Fish L,Tavazoie S,Tavazoie SF

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    pub_type: 杂志文章


    authors: Frischknecht F,Moreau V,Röttger S,Gonfloni S,Reckmann I,Superti-Furga G,Way M

    更新日期:1999-10-28 00:00:00

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    pub_type: 杂志文章


    authors: Sutrave P,Bonner TI,Rapp UR,Jansen HW,Patschinsky T,Bister K

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    pub_type: 杂志文章


    authors: Liu KH,Niu Y,Konishi M,Wu Y,Du H,Sun Chung H,Li L,Boudsocq M,McCormack M,Maekawa S,Ishida T,Zhang C,Shokat K,Yanagisawa S,Sheen J

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    pub_type: 杂志文章


    authors: Laval B,Cady SL,Pollack JC,McKay CP,Bird JS,Grotzinger JP,Ford DC,Bohm HR

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