Localization of the phosphorylation sites for different kinases in the microtubule-associated protein MAP2.


:The phosphorylation of microtubule-associated protein 2 (MAP2) by four different kinases was studied in vitro to determine whether MAP2 is phosphorylated in its tubulin binding region or in the microtubule projection portion. Fragments corresponding to both regions of MAP2 were produced not only by chymotrypsin or trypsin digestion, but also using pepsin, a broad chain-specificity protease, a result supporting previous notions of the two-domain structure of MAP2. The position of these two functional domains was determined with respect to the carboxy terminal of the molecule, by labeling MAP2 exclusively at the carboxy terminal and subjecting it to pepsin digestion. The results suggested that the projection region of MAP2 contained the carboxy terminal of the protein. A phosphorylation map was constructed by subjecting phosphorylated MAP2 to enzymatic digestion using Staphylococcus aureus V8 protease or to chemical cleavage using N-chlorosuccinimide. The results indicated that all four kinases phosphorylated MAP2 in a 42-kilodalton peptide that contained the tubulin binding region but differed in the level and localization of the sites at which they phosphorylated the projection of MAP2.


J Neurochem


Hernández MA,Wandosell F,Avila J




Has Abstract


1987-01-01 00:00:00












  • The astrocytic response to the dopaminergic denervation of the striatum.

    abstract::Increasing evidence suggests that the dopaminergic degeneration which characterizes Parkinson's disease starts in the striatal dopamine terminals and progresses retrogradely to the body of dopamine cells in the substantia nigra. The role of striatal astrocytes in the striatal initiation of the dopaminergic degeneratio...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Morales I,Sanchez A,Rodriguez-Sabate C,Rodriguez M

    更新日期:2016-10-01 00:00:00

  • Beta 2-adrenoreceptors stimulate c-fos transcription through multiple cyclic AMP- and Ca(2+)-responsive elements in cerebellar granular neurons.

    abstract::Neurons from the granular layer of the cerebellum express functional beta 2-adrenoreceptors (beta 2-ARs). We show that stimulation of beta 2-ARs with isoprenaline increases cyclic AMP (cAMP) production and stimulates transcription of genes containing the cAMP-responsive element (CRE; TGACGTCA). This effect is mediated...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Barthel F,Loeffler JP

    更新日期:1995-01-01 00:00:00

  • Desensitization of muscarinic receptor-coupled phosphoinositide hydrolysis in rat hippocampus: comparisons with the alpha 1-adrenergic response.

    abstract::In the presence of lithium, carbamylcholine chloride (carbachol) and epinephrine increase the accumulation of inositol monophosphate severalfold in hippocampal slices from the rat. The stimulation by carbachol (EC50, 31 microM) is mediated by muscarinic receptors, whereas the effects of epinephrine (EC50, 2 microM) ar...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lenox RH,Hendley D,Ellis J

    更新日期:1988-02-01 00:00:00

  • Treatment with normal prion protein delays differentiation and helps to maintain high proliferation activity in human embryonic stem cells.

    abstract::The normal cellular form of prion protein (PrP(C)) has been shown to exhibit a diverse range of biological activities. Several recent studies highlighted potential involvement of PrP(C) in embryogenesis or in regulating stem cell self-renewal and proliferation. In the current study, we employed human embryonic stem ce...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lee YJ,Baskakov IV

    更新日期:2010-07-01 00:00:00

  • Intracellular pH, lactate, and energy metabolism in neonatal brain during partial ischemia measured in vivo by 31P and 1H nuclear magnetic resonance spectroscopy.

    abstract::Sequential 31P and 1H nuclear magnetic resonance spectra were measured for neonatal piglets (n = 7) to determine the relationship between brain intracellular pH (pHi), lactate, and phosphorylated energy metabolites during partial ischemia. Simultaneous determinations of arterial and cerebral venous blood gases, pH, O2...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Corbett RJ,Laptook AR,Nunnally RL,Hassan A,Jackson J

    更新日期:1988-11-01 00:00:00

  • Seasonal decrease in thermogenesis and increase in vasoconstriction explain seasonal response to N6 -cyclohexyladenosine-induced hibernation in the Arctic ground squirrel (Urocitellus parryii).

    abstract::Hibernation is a seasonal phenomenon characterized by a drop in metabolic rate and body temperature. Adenosine A1 receptor agonists promote hibernation in different mammalian species, and the understanding of the mechanism inducing hibernation will inform clinical strategies to manipulate metabolic demand that are fun...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Frare C,Jenkins ME,McClure KM,Drew KL

    更新日期:2019-11-01 00:00:00

  • The role of tumor progression locus 2 protein kinase in glial inflammatory response.

    abstract::Tumor progression locus 2 (Tpl2)/cancer Osaka thyroid kinase is a newer member of MAP3K family that is now known for its essential role in tumor necrosis factor-aplha (TNFα) expression in macrophages, but its pro-inflammatory signaling, if any, in glia is unknown. When cultures of murine microglia and astrocytes were ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hirschhorn J,Mohanty S,Bhat NR

    更新日期:2014-03-01 00:00:00

  • Molecular identification and functional role of voltage-gated sodium channels in rat carotid body chemoreceptor cells. Regulation of expression by chronic hypoxia in vivo.

    abstract::We have assessed the expression, molecular identification and functional role of Na+ channels (Na(v)) in carotid bodies (CB) obtained from normoxic and chronically hypoxic adult rats. Veratridine evoked release of catecholamines (CA) from an in vitro preparation of intact CBs obtained from normoxic animals, the respon...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Caceres AI,Obeso A,Gonzalez C,Rocher A

    更新日期:2007-07-01 00:00:00

  • Postmortem changes of normetanephrine determined by a mass-fragmentographic technique in different rat brain areas.

    abstract::A specific and sensitive gas chromatographic-mass spectrometric assay for measurement of normetanephrine (NMN) in different rat brain regions is presented. The results show that hypothalamus contains the highest concentrations of the norepinephrine metabolite. An increase of NMN concentrations has been detected in ani...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Mocchetti I,De Angelis L,Racagni G

    更新日期:1981-12-01 00:00:00

  • Glutathione reductase mediates drug resistance in glioblastoma cells by regulating redox homeostasis.

    abstract::Glutathione (GSH) and GSH-related enzymes constitute the most important defense system that protects cells from free radical, radiotherapy, and chemotherapy attacks. In this study, we aim to explore the potential role and regulatory mechanism of the GSH redox cycle in drug resistance in glioblastoma multiforme (GBM) c...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Zhu Z,Du S,Du Y,Ren J,Ying G,Yan Z

    更新日期:2018-01-01 00:00:00

  • Effect of cholecystokinin octapeptide on endogenous amino acid release from the rat ventromedial nucleus of the hypothalamus and striatum.

    abstract::The sulphated octapeptide of cholecystokinin (CCK-8S) was found to cause a dose-dependent increase in the basal release of aspartate, glycine, and gamma-aminobutyric acid from the striatum and the ventromedial nucleus of the hypothalamus (VMH). No effect on amino acid release was observed after electrical (VMH) or pot...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Barnes S,Whistler HL,Hughes J,Woodruff GN,Hunter JC

    更新日期:1991-04-01 00:00:00

  • Increased AP-1 DNA binding activity in PC12 cells treated with lead.

    abstract::The possibility that the mechanism of lead neurotoxicity may be at the level of transcription was investigated in PC12 cells. In electrophoretic mobility gel shift assays Pb2+ was found to increase activator protein-1 complex (AP-1) DNA binding activity in PC12 cells; the increase was time- and concentration-dependent...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chakraborti T,Kim KA,Goldstein GG,Bressler JP

    更新日期:1999-07-01 00:00:00

  • GABA-opioid interactions in the globus pallidus: [D-Ala2]-Met-enkephalinamide attenuates potassium-evoked GABA release after nigrostriatal lesion.

    abstract::The motor signs of Parkinson's disease have been partly attributed to an overinhibition of the external globus pallidus (GP) that results from hyperactivity of striatopallidal GABA/enkephalinergic neurons. The goals of this study were to measure basal levels of extracellular fluid GABA in the GP of normal cats, 1-meth...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schroeder JA,Schneider JS

    更新日期:2002-08-01 00:00:00

  • Histamine increases phospholipid methylation and H2-receptor-adenylate cyclase coupling in rat brain.

    abstract::Histamine stimulated the enzymatic synthesis of phosphatidylcholine from phosphatidylethanolamine in crude synaptic membranes of rat brain containing the methyl donor S-adenosyl-L-methionine (SAM). In the presence of, but not in the absence of SAM, histamine increased cyclic AMP accumulation at the concentrations that...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ozawa K,Segawa T

    更新日期:1988-05-01 00:00:00

  • Alanine uptake and release by sympathetic ganglia of chicken embryos.

    abstract::Uptake and release of alanine were measured in lumbar sympathetic chains excised from embryos of white leghorn chickens, 14-15 days old, and incubated in a modified Eagle's minimum essential medium. In the presence of [U-14C]glucose, glucose carbon accumulated in alanine in the medium at a rate that increased when unl...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Larrabee MG

    更新日期:1984-09-01 00:00:00

  • A sensitive method for characterization of oligoclonal immunoglobulins in unconcentrated cerebrospinal fluid.

    abstract::Although isoelectric focusing has been used to demonstrate the presence of oligoclonal IgG in the CSF, the technique has not allowed detection of oligoclonal IgG in unconcentrated CSF. A new technique is reported, by which unconcentrated CSF is separated by isoelectric focusing, and the IgG bands are then detected by ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lasne Y,Benzerara O,Chazot G,Creyssel R

    更新日期:1981-05-01 00:00:00

  • Degradation of NAD by synaptosomes and its inhibition by nicotinamide mononucleotide: implications for the role of NAD as a synaptic modulator.

    abstract::We have found NAD to be rapidly degraded by extracellular enzymes present on intact rat brain synaptosomes. The enzyme involved had the specificity of an NADase cleaving the molecule at the nicotinamide-glycoside linkage and was inhibited by nicotinamide mononucleotide (NMN). This inhibitor did not displace specific b...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Snell CR,Snell PH,Richards CD

    更新日期:1984-12-01 00:00:00

  • SUMOylation of synaptic and synapse-associated proteins: An update.

    abstract::SUMOylation is a post-translational modification that regulates protein signalling and complex formation by adjusting the conformation or protein-protein interactions of the substrate protein. There is a compelling and rapidly expanding body of evidence that, in addition to SUMOylation of nuclear proteins, SUMOylation...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Henley JM,Seager R,Nakamura Y,Talandyte K,Nair J,Wilkinson KA

    更新日期:2020-06-15 00:00:00

  • High serotonin and 5-hydroxyindoleacetic acid levels in limbic brain regions in a rat model of depression: normalization by chronic antidepressant treatment.

    abstract::Although alterations in serotonin levels and neurotransmission are associated with depressive disorders and effective antidepressant therapy, the exact cause of these disorders and the mode of action of antidepressant drugs are poorly understood. In a genetic rat model of depression [Flinders sensitive line (FSL) rats...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Zangen A,Overstreet DH,Yadid G

    更新日期:1997-12-01 00:00:00

  • Inability of [125I]Sar1, Ile8-angiotensin II to move between the blood and cerebrospinal fluid compartments.

    abstract::The angiotensin II competitive antagonist [125I]-Sar1, Ile8-angiotensin II was not transported from the vascular space to the cerebroventricular space in either intact or nephrectomized rats. In addition [125I]Sar1, Ile8-angiotensin II lacked the capacity to move in the opposite direction over a 20-min collection peri...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Harding JW,Sullivan MJ,Hanesworth JM,Cushing LL,Wright JW

    更新日期:1988-02-01 00:00:00

  • Dexamethasone prevents hypoxia/ischemia-induced reductions in cerebral glucose utilization and high-energy phosphate metabolites in immature brain.

    abstract::We examined the potential importance of dexamethasone-mediated alterations in energy metabolism in providing protection against hypoxic-ischemic brain damage in immature rats. Seven-day-old rats (n = 165) that had been treated with dexamethasone (0.1 mg/kg, i.p.) or vehicle were assigned to control or hypoxic-ischemic...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tuor UI,Yager JY,Bascaramurty S,Del Bigio MR

    更新日期:1997-11-01 00:00:00

  • Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.

    abstract::The MRC OX-2 monoclonal antibody recognises membrane glycoproteins of Mr 41,000 in rat brain and 47,000 on thymocytes. It also reacts with follicular dendritic cells in lymphoid organs, endothelium, smooth muscle, and B-lymphocytes. Indirect immunoperoxidase staining of cryostat sections showed that OX-2 antigen was p...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Webb M,Barclay AN

    更新日期:1984-10-01 00:00:00

  • Effects of free fatty acids on synaptosomal amino acid uptake systems.

    abstract::The Na+-dependent synaptosomal uptakes of proline, aspartic acid, glutamic acid, and gamma-aminobutyric acid were strong inhibited by monounsaturated fatty acids. With oleic acid, half-maximal inhibition was observed at about 15 microM. The Na+-independent uptakes of leucine, phenylalanine, histidine, and valine were ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rhoads DE,Kaplan MA,Peterson NA,Raghupathy E

    更新日期:1982-05-01 00:00:00

  • Activation of choline acetyltransferase by vasoactive intestinal peptide.

    abstract::Addition of vasoactive intestinal peptide (VIP) to brain homogenates increased the activity of choline acetyltransferase (ChAT) but not that of acetylcholinesterase or glucose-6-phosphate dehydrogenase. Activity of ChAT was increased in the anterior hypothalamus and in the dorsal and ventral hippocampus, but not in th...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Luine VN,Rostene W,Rhodes J,McEwen BS

    更新日期:1984-04-01 00:00:00

  • gamma-Hydroxybutyric acid in subcellular fractions of rat brain.

    abstract::The presence of gamma-hydroxybutyric acid (GHB) in synaptosome-enriched fractions of rat brain was ascertained using a GLC technique. The stability of GHB in synaptosomes was evaluated by addition of various gamma-aminobutyric acid (GABA) transaminase (GABA-T) inhibitors, GHB, or ethosuximide to the homogenizing mediu...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Snead OC 3rd

    更新日期:1987-01-01 00:00:00

  • Diabetic neuropathy enhances voltage-activated Ca2+ channel activity and its control by M4 muscarinic receptors in primary sensory neurons.

    abstract::Painful neuropathy is one of the most serious complications of diabetes and remains difficult to treat. The muscarinic acetylcholine receptor (mAChR) agonists have a profound analgesic effect on painful diabetic neuropathy. Here we determined changes in T-type and high voltage-activated Ca(2+) channels (HVACCs) and th...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Cao XH,Byun HS,Chen SR,Pan HL

    更新日期:2011-11-01 00:00:00

  • Regionally selective and age-dependent alterations in benzodiazepine receptor binding in the genetically dystonic hamster.

    abstract::Previous pharmacological studies have indicated that impairment of GABAergic transmission may be involved in the pathophysiology of dystonia in the mutant dtsz hamster, i.e., a genetic animal model for idiopathic dystonia. In the present experiments, the kinetic constants of [3H]flumazenil binding to the benzodiazepin...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pratt GD,Richter A,Möhler H,Löscher W

    更新日期:1995-05-01 00:00:00

  • Hyaluronidase inhibition accelerates functional recovery from stroke in the mouse brain.

    abstract::Perineuronal nets (PNNs) are presumed to limit plasticity in adult animals. Ischaemic stroke results in the massive breakdown of PNNs resulting in rejuvenating states of neuronal plasticity, but the mechanisms of this phenomenon are largely unknown. As hyaluronic acid (HA) is the structural backbone of PNNs, we hypoth...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Katarzyna Greda A,Nowicka D

    更新日期:2020-12-20 00:00:00

  • Gene-environment interactions in sporadic Parkinson's disease.

    abstract::Much has been learned in recent years about the genetics of familial Parkinson's disease. However, far less is known about those malfunctioning genes which contribute to the emergence and/or progression of the vast majority of cases, the 'sporadic Parkinson's disease', which is the focus of our current review. Drastic...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Benmoyal-Segal L,Soreq H

    更新日期:2006-06-01 00:00:00

  • Tyrosine hydroxylase activity is regulated by two distinct dopamine-binding sites.

    abstract::Tyrosine hydroxylase (TH), the rate-limiting enzyme in the biosynthesis of the catecholamines dopamine, noradrenaline and adrenaline, is regulated acutely by feedback inhibition by the catecholamines and relief of this inhibition by phosphorylation of serine 40 (Ser40). Phosphorylation of serine 40 abolishes the bindi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Gordon SL,Quinsey NS,Dunkley PR,Dickson PW

    更新日期:2008-08-01 00:00:00