Clostridial collagenase. A chemoattractant for human neutrophils.


:Leukocyte chemoattractants markedly alter the morphology and membrane functions of leukocytes. Bacterial collagenase causes a change in cell shape similar to that seen with the leukocyte chemoattractant, f-Met-Leu-Phe, and also promotes capping of concanavalin A. Human neutrophils in suspension or adherent to cover glasses were exposed to clostridial collagenase (10-250 units/ml) for up to 30 min at 37 degrees C and then fixed. Collagenase (125 units/ml) caused more than 85% of PMNs to assume an asymmetric or motile morphology even in the presence of 1% gelatin or 10 mg/ml bovine serum albumin. Trypsin alone (0.01-1%) did not induce a shape change. A similar morphology was seen in some untreated PMNs (less than 5% of all cells) and is characteristic of f-Met-Leu-Phe-treated cells (more than 90%). Collagenase inhibitors (i.e., reduced glutathione, cysteine, and acid-soluble collagen), however, prevented the shape change induced by collagenase but not by f-Met-Leu-Phe. At 4 degrees C, fluorescein-Con A (20 micrograms/ml) bound uniformly to both untreated and collagenase-treated cells. Upon further incubation at 37 degrees C, Con A was internalized over the entire cell periphery of the rounded, untreated cells but on collagenase-treated PMNs was rapidly gathered into a cap overlying the uropod or protuberant region of cytoplasm where it was subsequently internalized. Checkerboard Boyden chamber assays showed clostridial collagenase to be chemokinetic and chemotactic for human PMNs. In receptor binding experiments, the clostridial collagenase preparation competed poorly with [125I]formylhexapeptide for binding to PMN formylpeptide receptors (less than 15% reduction in binding at 200 units/ml collagenase). Thus, collagenase does not seem to interact strongly with the neutrophil formylpeptide receptor and may stimulate PMN motility by interacting at an altogether different site.






Walter RJ




Has Abstract


1986-12-01 00:00:00












  • Simvastatin Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation by Suppressing the NF-kB and MAPK Pathways in Nucleus Pulposus Cells.

    abstract::Statins are widely used hypocholesterolemic drugs that block the mevalonate pathway. Some studies have shown that statins may have the potential to inhibit intervertebral disk (IVD) degeneration (IDD). Interleukin (IL)-1β, a catabolic cytokine, is a key regulator of IDD. This study aimed to investigate the mechanism u...


    pub_type: 杂志文章


    authors: Tu J,Li W,Zhang Y,Wu X,Song Y,Kang L,Liu W,Wang K,Li S,Hua W,Yang C

    更新日期:2017-06-01 00:00:00

  • Neutrophil-mediated proteolysis. Differential roles for cathepsin G and elastase.

    abstract::In this study, we assessed the underlying mechanisms by which proteinases released from activated neutrophils mediate fibronectin degradation. Purified human neutrophils (1 x 10(6)) were incubated for 1 hr with 1 microM PMA in the absence or presence of different proteinase inhibitors in 96-well microtiter plates that...


    pub_type: 杂志文章


    authors: Kubes P,Smith R,Grisham MD,Granger DN

    更新日期:1993-06-01 00:00:00

  • Reduced serum B7-H3 levels in patients with ankylosing spondylitis.

    abstract::This study aimed to investigate molecule B7-H3 expression profiles of patients with ankylosing spondylitis (AS) and the clinical significance of B7-H3 in the pathogenesis of AS. Serum B7-H3 levels were measured by ELISA in patients with AS and healthy controls. The expression of B7-H3 protein and mRNA on CD14+ monocyt...


    pub_type: 杂志文章


    authors: Yan R,Hu Y,Yu P,Wen H,Feng P,Yang S

    更新日期:2015-01-01 00:00:00

  • Autologous versus allogeneic T cell-stimulated IL-6 production by dermal fibroblasts.

    abstract::T cells adhere to human dermal fibroblasts (HDF). This cellular interaction leads to a pronounced secretion of the proinflammatory cytokines IL-6 and IL-8 via a juxtacrine stimulation induced by HDF-associated IL-1. Upon stimulation, fibroblasts express various surface proteins such as MCH-I molecules, which may inter...


    pub_type: 杂志文章


    authors: Spörri B,Bickel M,Limat A,Waelti ER,Hunziker T,Wiesmann UN

    更新日期:1997-08-01 00:00:00

  • Granulocytes utilize different energy sources for movement and phagocytosis.

    abstract::Granulocytes depend on anaerobic glycolysis for the energy required for chemotaxis, phagocytosis, and microbial killing. Two potential sources of the needed glucose are available: exogenous glucose and intracellular glycogen. These studies demonstrate that chemotaxin-induced movement of granulocytes induces accelerate...


    pub_type: 杂志文章


    authors: Weisdorf DJ,Craddock PR,Jacob HS

    更新日期:1982-09-01 00:00:00

  • Activated human peripheral blood neutrophils produce epithelial injury and fibronectin breakdown in vitro.

    abstract::The ability of peripheral blood neutrophils to injure elements of the alveolar septum was assessed. Experiments consisted of activating previously isolated neutrophils (PMNs) with a soluble (phorbol myristate acetate, PMA) and a particulate (zymosan) trigger, and measuring detachment of 51Cr-labeled epithelial cells. ...


    pub_type: 杂志文章


    authors: Brown DM,Brown GM,Macnee W,Donaldson K

    更新日期:1992-02-01 00:00:00

  • Influence of hyperoxia and mechanical ventilation in lung inflammation and diaphragm function in aged versus adult rats.

    abstract::Although assist ventilation with FIO2 0.21 is the preferable mode of ventilation in the intensive care unit, sometimes controlled ventilation with hyperoxia is needed. But the impact of this setting has not been extensively studied in elderly subjects. We hypothesized that a high fraction of inspired oxygen (FiO(2)) a...


    pub_type: 杂志文章


    authors: Andrade PV,dos Santos JM,Silva HC,Wilbert DD,Cavassani SS,Oliveira-Júnior IS

    更新日期:2014-04-01 00:00:00

  • Deficiency in C3b receptors on neutrophils of patients with chronic granulomatous disease and hyperimmunoglobulin-E recurrent infection (Job's) syndrome.

    abstract::C3b receptor (CR1) expression by neutrophils (PMNs) and erythrocytes (Es) from patients with chronic granulomatous disease (CGD) or with hyper-IgE, frequent infection (Job's) syndrome was compared with that of control subjects. The control subjects consisted of one group of patients with infections and a second group ...


    pub_type: 杂志文章


    authors: Gaither TA,Gallin JI,Iida K,Nussenzweig V,Frank MM

    更新日期:1984-12-01 00:00:00

  • Downregulation of ferroportin 1 expression in hFOB1.19 osteoblasts by hepcidin.

    abstract::The present study was designed to address the relationship between iron homeostasis and bone metabolism. Cultured hFOB 1.19 osteoblasts were incubated with selected concentrations of hepcidin (50, 100, and 200 nmol/L) for 20 h, harvested for extraction of total RNA and proteins, and the expression of ferroportin 1 was...


    pub_type: 杂志文章


    authors: Xu Y,Zhang W,Zhang P,Xiao L,Wang A,Sirois P,Li K

    更新日期:2012-06-01 00:00:00

  • Elevated plasma platelet activating factor, platelet activating factor acetylhydrolase levels and risk of coronary heart disease or blood stasis syndrome of coronary heart disease in Chinese: a case control study: a case-control study.

    abstract::The purpose of the study was to explore the association between plasma platelet activating factor (PAF) and platelet activating factor acetylhydrolase (PAF-AH) levels and risk of coronary heart disease (CHD) or blood stasis syndrome (BSS) of CHD. Questionnaire, routine clinical assays and plasma levels of PAF, PAF-AH ...


    pub_type: 杂志文章


    authors: Zheng GH,Xiong SQ,Mei LJ,Chen HY,Wang T,Chu JF

    更新日期:2012-08-01 00:00:00

  • Cell-cell contacts with epithelial cells modulate the phenotype of human macrophages.

    abstract::Interactions of macrophages with epithelium represent one of the pathways involved in regulating local immune mechanisms. We studied the effect of cell-cell contact with an epithelial monolayer on the phenotype of macrophages. Human monocytes and THP-1 macrophages were co-cultured with monolayers of human bronchial ep...


    pub_type: 杂志文章


    authors: Striz I,Slavcev A,Kalanin J,Jaresová M,Rennard SI

    更新日期:2001-08-01 00:00:00

  • The Influence of a Xanthine-Catechin Chemical Matrix on in vitro Macrophage-Activation Triggered by Antipsychotic Ziprasidone.

    abstract::Ziprasidone (ZIP) is an effective antipsychotic with low side effects than other second-generation antipsychotics. Despite this, there are reports of adverse events and previous studies associating the use of ZIP the inflammatory response. It is possible to infer that bioactive molecules present in some foods could at...


    pub_type: 杂志文章


    authors: Duarte T,Barbisan F,da Cunha BSN,Azzolin VF,Turra BO,Duarte MMMF,da Cruz Jung IE,Ribeiro EE,do Prado-Lima PA,da Cruz IBM

    更新日期:2019-06-01 00:00:00

  • Induction of neutrophil Mac-1 integrin expression and superoxide production by the medicinal plant extract gossypol.

    abstract::Gossypol is present in antiinflammatory poultices made from the medicinal tree Thespesia populnea. Isolated human neutrophils exposed to 3-20 microM gossypol for 15-90 min were assayed in vitro for superoxide production and surface expression of Mac-1 (CD11b/CD18). Gossypol increased superoxide production in a time- a...


    pub_type: 杂志文章


    authors: Benhaim P,Mathes SJ,Hunt TK,Scheuenstuhl H,Benz CC

    更新日期:1994-10-01 00:00:00

  • Bacterial lipopolysaccharide-stimulated nitric oxide generation is unrelated to concurrent cytotoxicity of bovine alveolar macrophages.

    abstract::Nitric Oxide (NO) is a reactive metabolite produced by stimulated macrophages, and it has been demonstrated to exert cytotoxic actions on a number of microbes, parasites, and tumor cells. In addition, NO has been reported to have an autotoxic effect on murine macrophages, its site of synthesis. We have investigated th...


    pub_type: 杂志文章


    authors: Bochsler PN,Mason GL,Olchowy TW,Yang Z

    更新日期:1996-04-01 00:00:00

  • Downregulation of CCR5 expression on the peripheral blood CD8+ T cells of southeastern Iranian patients with chronic hepatitis B infection.

    abstract::Studies indicated that CC receptor 5 (CCR5), as a receptor for CC ligand 3, CCL4, and CCL5, plays important roles in the recruitment of T cytotoxic lymphocytes to the liver of chronic HBV (CHB)-infected patients. The main purpose of this study was to investigate the expression levels of CCR5 on the CD8(+) T lymphocyte...


    pub_type: 杂志文章


    authors: Ahmadabadi BN,Hassanshahi G,Khoramdelazad H,Mirzaei V,Sajadi SM,Hajghani M,Khodadadi H,Pourali R,Arababadi MK,Kennedy D

    更新日期:2013-02-01 00:00:00

  • The anti-inflammatory mechanism of heme oxygenase-1 induced by hemin in primary rat alveolar macrophages.

    abstract::Alveolar macrophages (AMs) can initiate lung inflammation by producing pro-inflammatory cytokines and chemokines, but they participate actively in the prevention of inflammation during acute lung injury (ALI). Heme oxygenase-1 (HO-1) is mainly expressed in AMs and has anti-inflammatory properties in ALI, but the anti-...


    pub_type: 杂志文章


    authors: Hualin C,Wenli X,Dapeng L,Xijing L,Xiuhua P,Qingfeng P

    更新日期:2012-06-01 00:00:00

  • Correction to: Inhibition of C3a/C3aR Axis in Diverse Stages of Ulcerative Colitis Affected the Prognosis of UC by Modulating the Pyroptosis and Expression of Caspase-11.

    abstract::Following publication of our article [1], we noticed an error in Fig. 1. ...


    pub_type: 已发布勘误


    authors: Zhang X,Chen Y,Yu S,Jin B,Liu W

    更新日期:2020-11-10 00:00:00

  • Inflammatory processes and effects of continuous positive airway pressure (CPAP) in overlap syndrome.

    abstract::We aimed to compare serum levels of the inflammatory mediators including C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), and asymmetric dimethylarginine (ADMA) in chronic obstructive pulmonary disease (COPD), obstructive sleep apnea syndrome (OSAS), and their coexistence called overlap syndrome (OVS). In ad...


    pub_type: 杂志文章


    authors: Nural S,Günay E,Halici B,Celik S,Ünlü M

    更新日期:2013-02-01 00:00:00

  • Astragalin Attenuates Allergic Inflammation in a Murine Asthma Model.

    abstract::The present study aimed to determine the protective effects and the underlying mechanisms of astragalin (AG) on ovalbumin (OVA)-induced allergic inflammation in a mouse model of allergic asthma. Our study demonstrated that AG inhibited OVA-induced increases in eosinophil count; IL-4, IL-5, IL-13, and IgE were recovere...


    pub_type: 杂志文章


    authors: Liu J,Cheng Y,Zhang X,Zhang X,Chen S,Hu Z,Zhou C,Zhang E,Ma S

    更新日期:2015-10-01 00:00:00

  • Cytokines increase proliferation of human intestinal smooth muscle cells: possible role in inflammation-induced stricture formation.

    abstract::Crohn's disease is an idiopathic, chronic inflammation of the gastrointestinal tract that causes narrowing and stricturing of primarily the small and large intestine. Although the mechanism(s) by which chronic inflammation promotes stricture formation remain to be defined, it does appear to be associated histologicall...


    pub_type: 杂志文章


    authors: Owens MW,Grisham MB

    更新日期:1993-08-01 00:00:00

  • Pathogenesis of papain-induced emphysema in the hamster.

    abstract::In an effort to determine the mechanism of papain action in causing an emphysema-like lesion in hamsters, the number and types of cells and the activities of two lysosomal enzymes in the lung were determined after papain exposure. Three and four weeks after a 3-h exposure to an aerosol of 3% papain the following alter...


    pub_type: 杂志文章


    authors: Ackerman NR,Corkey R,Perkins D

    更新日期:1978-03-01 00:00:00

  • Priming of human neutrophil functions by tumor necrosis factor: enhancement of superoxide anion generation, degranulation, and chemotaxis to chemoattractants C5a and F-Met-Leu-Phe.

    abstract::Recombinant human tumor necrosis factor-alpha (rTNF) stimulated increased generation of superoxide anion (O2-) by human neutrophils in a concentration-dependent fashion. Preincubation of human neutrophils with rTNF (2.2-2200 units/ml) for 10 min enhanced the subsequent generation of O2- in response to C5a and f-Met-Le...


    pub_type: 杂志文章


    authors: Bajaj MS,Kew RR,Webster RO,Hyers TM

    更新日期:1992-06-01 00:00:00

  • Interleukin-1 beta induces expression of cyclooxygenase-2 mRNA in human gingival fibroblasts.

    abstract::The effect of interleukin-1 beta (IL-1 beta) on the expression of cyclooxygenase-1 and -2 (COX-1 and COX-2) mRNA and its relation to prostaglandin E2 (PGE2) biosynthesis in human gingival fibroblasts was studied. IL-1 beta increased levels of mRNA for COX-2 whereas the COX-1 mRNA level was unaffected. The increased CO...


    pub_type: 杂志文章


    authors: Yucel-Lindberg T,Ahola H,Nilsson S,Carlstedt-Duke J,Modéer T

    更新日期:1995-10-01 00:00:00

  • Levels of messenger ribonucleic acids for plasma proteins in rat liver during acute experimental inflammation.

    abstract::The levels of mRNA for plasma proteins and for metallothionein in rat liver during the acute-phase response were studied by hybridization to specific cDNA probes. The mRNA for alpha 2-macroglobulin, the beta-chain of fibrinogen, alpha 1-acid glycoprotein (so-called acute-phase reactants) reached a maximum level betwee...


    pub_type: 杂志文章


    authors: Schreiber G,Aldred AR,Thomas T,Birch HE,Dickson PW,Tu GF,Heinrich PC,Northemann W,Howlett GJ,de Jong FA

    更新日期:1986-03-01 00:00:00

  • Analysis of the PKC-gamma-related immunocrossreactive region of a novel leukocyte protein gamma-rp.

    abstract::The newly discovered gamma-PKC-related-protein of human leukocytes (gamma-rp) crossreacts with a polyclonal antibody preparation originally designed to be specific for PKC-gamma (gammaMb-Ab). As this antibody is currently the only suitable probe for gamma-rp, we sought to characterize the binding of the two proteins. ...


    pub_type: 杂志文章


    authors: Smallwood JI,Malawista SE

    更新日期:1999-08-01 00:00:00

  • Transient endotoxemia during burn wound revision causes leukocyte beta 2 integrin up-regulation and cytokine release.

    abstract::After severe burns, a wound revision is often done to remove devitalized tissue and minimize bacterial growth. After such revision, the patient may show signs of sepsis. In a group of burned patients we found a transient endotoxemia, and a subsequent leukocyte activation, monitored as increased expression of the beta ...


    pub_type: 杂志文章


    authors: Ljunghusen O,Berg S,Hed J,Lundahl J,Nettelblad H,Sjögren F,Stendahl O

    更新日期:1995-08-01 00:00:00

  • IL-10RB rs2834167 (A/G) polymorphism is associated with the susceptibility to systemic lupus erythematosus: evidence from a study in Chinese Han population.

    abstract::Interleukin-10 (IL-10) is a pleiotropic cytokine and plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). Unlike IL-10 protein, few studies have focused on the potential association between IL-10 receptor (IL-10R) and SLE. The purpose of this study was to examine the association of a sing...


    pub_type: 杂志文章


    authors: Peng H,Liu CY,Zhou M,Wen PF,Zhang M,Qiu LJ,Ni J,Liang Y,Pan HF,Ye DQ

    更新日期:2013-12-01 00:00:00

  • Rosmarinic Acid Mitigates Lipopolysaccharide-Induced Neuroinflammatory Responses through the Inhibition of TLR4 and CD14 Expression and NF-κB and NLRP3 Inflammasome Activation.

    abstract::The excessive activation of microglia plays a key role in the pathogenesis of neurodegenerative diseases. The neuroprotective properties of rosmarinic acid have been reported in a variety of disease models both in vitro and in vivo; however, the mechanism underlying its anti-neuroinflammatory activity has not been cle...


    pub_type: 杂志文章


    authors: Wei Y,Chen J,Hu Y,Lu W,Zhang X,Wang R,Chu K

    更新日期:2018-03-01 00:00:00

  • Protective and Therapeutic Effects of Engeletin on LPS-Induced Acute Lung Injury.

    abstract::Acute lung injury (ALI) is a serious disease with morbidity and mortality in patients. Engeletin (dihydrokaempferol 3-rhamnoside) is a flavanonol glycoside. It can be found in the skin of white grapes and white wine and is widely distributed in southeast Asia. In our study, we evaluated the protective and therapeutic ...


    pub_type: 杂志文章


    authors: Jiang X,Chen L,Zhang Z,Sun Y,Wang X,Wei J

    更新日期:2018-08-01 00:00:00

  • Maresin 1 Maintains the Permeability of Lung Epithelial Cells In Vitro and In Vivo.

    abstract::Previous reports showed that Maresin 1 (MaR1) possessed organ protection effects and could attenuate acute lung injury. Here, we aim to figure out whether MaR1 can maintain the permeability of lung epithelial cells by regulating the expression of tight junction protein during lung injury. Monolayer of murine lung epit...


    pub_type: 杂志文章


    authors: Chen L,Liu H,Wang Y,Xia H,Gong J,Li B,Yao S,Shang Y

    更新日期:2016-12-01 00:00:00