Clostridial collagenase. A chemoattractant for human neutrophils.


:Leukocyte chemoattractants markedly alter the morphology and membrane functions of leukocytes. Bacterial collagenase causes a change in cell shape similar to that seen with the leukocyte chemoattractant, f-Met-Leu-Phe, and also promotes capping of concanavalin A. Human neutrophils in suspension or adherent to cover glasses were exposed to clostridial collagenase (10-250 units/ml) for up to 30 min at 37 degrees C and then fixed. Collagenase (125 units/ml) caused more than 85% of PMNs to assume an asymmetric or motile morphology even in the presence of 1% gelatin or 10 mg/ml bovine serum albumin. Trypsin alone (0.01-1%) did not induce a shape change. A similar morphology was seen in some untreated PMNs (less than 5% of all cells) and is characteristic of f-Met-Leu-Phe-treated cells (more than 90%). Collagenase inhibitors (i.e., reduced glutathione, cysteine, and acid-soluble collagen), however, prevented the shape change induced by collagenase but not by f-Met-Leu-Phe. At 4 degrees C, fluorescein-Con A (20 micrograms/ml) bound uniformly to both untreated and collagenase-treated cells. Upon further incubation at 37 degrees C, Con A was internalized over the entire cell periphery of the rounded, untreated cells but on collagenase-treated PMNs was rapidly gathered into a cap overlying the uropod or protuberant region of cytoplasm where it was subsequently internalized. Checkerboard Boyden chamber assays showed clostridial collagenase to be chemokinetic and chemotactic for human PMNs. In receptor binding experiments, the clostridial collagenase preparation competed poorly with [125I]formylhexapeptide for binding to PMN formylpeptide receptors (less than 15% reduction in binding at 200 units/ml collagenase). Thus, collagenase does not seem to interact strongly with the neutrophil formylpeptide receptor and may stimulate PMN motility by interacting at an altogether different site.






Walter RJ




Has Abstract


1986-12-01 00:00:00












  • Synergism between interleukin-1 beta and tumor necrosis factor-alpha in production by 3T3 cells of a chemotactic factor for rat polymorphonuclear leukocytes.

    abstract::Recombinant human interleukin-1 beta (IL-1) and tumor necrosis factor-alpha (TNF) synergistically stimulated BALB/c 3T3 cells to produce a chemotactic factor for rat polymorphonuclear leukocytes (PMNs), whereas an addition of 10(-11)-10(-8) M IL-1 or TNF alone to the cell culture resulted in a slight increase in the p...


    pub_type: 杂志文章


    authors: Nakagawa H,Miyai H,Hirata M,Watanabe K,Onuma I

    更新日期:1989-10-01 00:00:00

  • Platelet factor 4 inhibits IL-17/Stat3 pathway via upregulation of SOCS3 expression in melanoma.

    abstract::Platelet factor 4 (PF4) was the first discovered CXC chemokine and is found in platelet granules at very high concentration. Now, it is becoming increasingly evident that PF4 actively participates in inflammation and immune response. Recent paper demonstrated that PF4 limits the development and response of the Th17 ce...


    pub_type: 杂志文章


    authors: Fang S,Liu B,Sun Q,Zhao J,Qi H,Li Q

    更新日期:2014-10-01 00:00:00

  • Ability of recombinant human catalase to suppress inflammation of the murine lung induced by influenza A.

    abstract::Influenza A virus pandemics and emerging antiviral resistance highlight the urgent need for novel generic pharmacological strategies that reduce both viral replication and inflammation of the lung. We have previously investigated the therapeutic efficacy of recombinant human catalase (rhCAT) against viral pneumonia in...


    pub_type: 杂志文章


    authors: Shi X,Shi Z,Huang H,Zhu H,Zhou P,Zhu H,Ju D

    更新日期:2014-06-01 00:00:00

  • Calgranulin B (S100A9) levels in bronchoalveolar lavage fluid of patients with interstitial lung diseases.

    abstract::Calgranulins are small calcium-binding proteins with several immunological functions involved in inflammatory processes. Calgranulin A is reported to be mainly associated with acute inflammation while calgranulin B seems to play a role in chronic inflammatory disorders. In this study we used a proteomic approach to an...


    pub_type: 杂志文章


    authors: Bargagli E,Olivieri C,Prasse A,Bianchi N,Magi B,Cianti R,Bini L,Rottoli P

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  • Postnatal development of hepatic innate immune response.

    abstract::The liver is an immunocompetent organ that plays a key role in the immune response to infections, and the development of hepatic immune function during early postnatal stages has not been thoroughly characterized. This study analyzed the constitutive expression of complement factors, namely C3 and C9, and pattern reco...


    pub_type: 杂志文章


    authors: Le Rouzic V,Corona J,Zhou H

    更新日期:2011-12-01 00:00:00

  • Propofol Protects Rats and Human Alveolar Epithelial Cells Against Lipopolysaccharide-Induced Acute Lung Injury via Inhibiting HMGB1 Expression.

    abstract::High-mobility group box 1 (HMGB1) plays a key role in the development of acute lung injury (ALI). Propofol, a general anesthetic with anti-inflammatory properties, has been suggested to be able to modulate lipopolysaccharide (LPS)-induced ALI. In this study, we investigated the effects of propofol on the expression of...


    pub_type: 杂志文章


    authors: Wang X,Liu C,Wang G

    更新日期:2016-06-01 00:00:00

  • Oligonucleotide microarray and QRT-PCR study of adhesion protein gene expression in acute coronary syndrome patients.

    abstract::Cardiovascular diseases, including acute coronary syndrome (ACS), are the leading cause of death among humans. Adhesion proteins, owing to their involvement in the initiation and progression of atherosclerotic lesions, contribute to the progression of coronary disease and ACS occurrence. Considering ambiguosity of res...


    pub_type: 杂志文章


    authors: Dabek J,Ligus J,Szota J

    更新日期:2010-12-01 00:00:00

  • Heat shock and proinflammatory stressors induce differential localization of heat shock proteins in human monocytes.

    abstract::Heat shock (HS) proteins (HSP) are a family of molecular chaperones induced by environmental stresses such as oxidative injury, and contribute to protection from and adaptation to cellular stress. We investigated in human monocytes the expression and subcellular distribution of hsp70 and hsc70 after HS and inflammatio...


    pub_type: 杂志文章


    authors: Mariéthoz E,Jacquier-Sarlin MR,Multhoff G,Healy AM,Tacchini-Cottier F,Polla BS

    更新日期:1997-12-01 00:00:00

  • Rho kinase Blockade Ameliorates DSS-Induced Ulcerative Colitis in Mice Through Dual Inhibition of the NF-κB and IL-6/STAT3 Pathways.

    abstract::Inflammatory bowel disease (IBD) has received much attention due to its increasing worldwide incidence and potential increased risk of colorectal cancer. The protective function of a Rho-associated protein kinase inhibitor (Y-27632) against 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced mouse colitis has been prov...


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    authors: Wang Y,Duan X,Liu X,Liu Y,Fan H,Xu M,Chen Q,Tang Q

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  • Protective Effect of Mitogen- and Stress-Activated Protein Kinase on the Rats with Focal Ischemia-Reperfusion Injury.

    abstract::Mitogen- and stress-activated protein kinase (MSK) is a recently identified nuclear cAMP-regulated enhancer B (CREB) and histone H3 kinase that responds to both mitogen- and stress-activated protein kinases. This study was designed to investigate the protective effect of MSK on the rats with focal ischemia-reperfusion...


    pub_type: 杂志文章


    authors: Zhou Y,Gao G,Li Z,Jiang L

    更新日期:2019-12-01 00:00:00

  • Manganese-containing superoxide-dismutase deficiency in polymorphonuclear leukocytes of adults with rheumatoid arthritis.

    abstract::Superoxide dismutase (SOD) is known to regulate the level of superoxide radicals inside cells. The purpose of this work was to investigate the role of SOD activity in tissue damage produced by superoxide radicals. SOD was measured in polymorphonuclear cells of patients with rheumatoid arthritis and controls. The disti...


    pub_type: 杂志文章


    authors: Pasquier C,Mach PS,Raichvarg D,Sarfati G,Amor B,Delbarre F

    更新日期:1984-03-01 00:00:00

  • MicroRNA-30a Targets ATG5 and Attenuates Airway Fibrosis in Asthma by Suppressing Autophagy.

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    authors: Li BB,Chen YL,Pang F

    更新日期:2020-02-01 00:00:00

  • Polymerase chain reaction amplification of messages for growth factors in cells from human bronchoalveolar lavage fluids.

    abstract::Genetic messages for polypeptide growth factors were assessed in human alveolar macrophages, obtained by bronchoalveolar lavage (BAL) from normal subjects (N = 3) and from patients with pneumonia (N = 3), pulmonary lymphoma (N = 3), and idiopathic pulmonary fibrosis (N = 3). Complementary DNAs (cDNAs) were prepared by...


    pub_type: 杂志文章


    authors: Adolff CH,Golden JA,Kennedy PW,Goetzl EJ,Turck CW

    更新日期:1991-08-01 00:00:00

  • Anti-inflammatory and In Silico Docking Studies of Heterophragma adenophyllum Seem Stem Constituents.

    abstract::Heterophragma adenophyllum is a traditional medicinal plant that has been used as anti-inflammatory and to relief muscular tension. In the current research, four isolated constitutes namely lapacho (1), peshawaraquinone (2), indanone derivatives (3), α-lapachone (4) of H. adenophyllum were tested for anti-inflammatory...


    pub_type: 杂志文章


    authors: Abu-Izneid T,Shah ZA,Rauf A,Wadood A,Bawazeer S,Muhammad N,El-Esawi MA,Alhumaydhi FA,Aljohani ASM,El-Sharkawy E,Mubarak MS,Isayeva K,Shariati MA

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  • Effects of Naringenin on inflammation in complete freund's adjuvant-induced arthritis by regulating Bax/Bcl-2 balance.

    abstract::Naringin (COG) is a flavanone with various bioactivities including an expectorant effect, antitussive effect, and inhibitory effect on asthma and acute lung injury. The aims of the present study were to investigate the antiarthritis activities of COG and elucidate the underlying mechanisms with regard to its molecular...


    pub_type: 杂志文章


    authors: Zhu L,Wang J,Wei T,Gao J,He H,Chang X,Yan T

    更新日期:2015-02-01 00:00:00

  • Baicalein inhibits interleukin-1β-induced proliferation of human rheumatoid arthritis fibroblast-like synoviocytes.

    abstract::Baicalein shows anti-inflammatory effects in human rheumatoid arthritis fibroblast-like synoviocytes (RAFLS). Considering its anti-proliferatory effects on various cancer cells, we investigated the effects of baicalein on interleukin-1 beta (IL-1β)-induced proliferation of human RAFLS. Cell proliferation was examined ...


    pub_type: 杂志文章


    authors: Chen S,Yang Y,Feng H,Wang H,Zhao R,Liu H

    更新日期:2014-02-01 00:00:00

  • Protective Effects of Isoliquiritigenin on LPS-Induced Acute Lung Injury by Activating PPAR-γ.

    abstract::Isoliquiritigenin (ILG), a major ingredient of licorice, has been reported to have anti-oxidative and anti-inflammatory effects. The aim of this study was to investigate the protective effects of ILG on lung injury using an animal model of lipopolysaccharide (LPS)-induced acute lung injury (ALI). Male BALB/c mice were...


    pub_type: 杂志文章


    authors: Zhang W,Wang G,Zhou S

    更新日期:2018-08-01 00:00:00

  • Melanoma Differentiation-Associated Gene 5 Positively Modulates TNF-α-Induced CXCL10 Expression in Cultured HuH-7 and HLE Cells.

    abstract::The molecular mechanisms of innate immunity are closely associated with the development of non-alcoholic fatty liver disease (NAFLD). TNF-α is a key cytokine involved in the pathogenesis of metabolic inflammation like NAFLD. Melanoma differentiation-associated gene 5 (MDA5) is a member of the intracellular RNA helicas...


    pub_type: 杂志文章


    authors: Kawaguchi S,Sakuraba H,Haga T,Matsumiya T,Seya K,Endo T,Sawada N,Iino C,Kikuchi H,Hiraga H,Fukuda S,Imaizumi T

    更新日期:2019-12-01 00:00:00

  • Pharmacological Evaluation of TAK-828F, a Novel Orally Available RORγt Inverse Agonist, on Murine Colitis Model.

    abstract::IL-17-producing Th17 cells and IFN-γ and IL-17 double-producing Th1/17 cells have been identified as the pathogenic cells in inflammatory bowel disease (IBD). Retinoic acid-related orphan receptor γt (RORγt) is a master regulator for the differentiation and activation of Th17 and Th1/17 cells. We discovered a novel or...


    pub_type: 杂志文章


    authors: Igaki K,Nakamura Y,Komoike Y,Uga K,Shibata A,Ishimura Y,Yamasaki M,Tsukimi Y,Tsuchimori N

    更新日期:2019-02-01 00:00:00

  • In vitro effects of omega-3 fatty acids on neutrophil intracellular calcium homeostasis and receptor expression for FMLP and LTB4.

    abstract::Diets enriched in omega-3 fatty acids exert antiinflammatory properties by suppressing some neutrophil (PMN) functions. Changes in cytosolic Ca2+ concentration, [Ca2+]i, are important for PMN activation and are in part regulated by membrane Ca2+ ATPases. Since membrane proteins are influenced by their lipid environmen...


    pub_type: 杂志文章


    authors: Georgilis K,Klempner MS

    更新日期:1988-10-01 00:00:00

  • Physio-pharmacological Investigations About the Anti-inflammatory and Antinociceptive Efficacy of (+)-Limonene Epoxide.

    abstract::D-limonene epoxidation generates (+)-limonene epoxide, an understudied compound in the pharmacologically point of view. Herein, we investigated the anti-inflammatory and antinociceptive potentialities of (+)-limonene epoxide and suggested a mechanism of action. The anti-inflammatory potential was analyzed using agents...


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    authors: de Almeida AA,Silva RO,Nicolau LA,de Brito TV,de Sousa DP,Barbosa AL,de Freitas RM,Lopes LD,Medeiros JR,Ferreira PM

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  • Purification and characterization of calpain from human polymorphonuclear leukocytes.

    abstract::Recent studies have demonstrated that a calcium-sensitive protease converts Ca2+/phospholipid-dependent protein kinase C to a Ca2+/phospholipid-independent form during the activation of human neutrophils. In this paper, the results of the purification and characterization of a calcium-dependent cytosolic protease from...


    pub_type: 杂志文章


    authors: Legendre JL,Jones HP

    更新日期:1988-02-01 00:00:00

  • Characterization of a 130,000-dalton glycoprotein isolated from pulmonary secretions of patients with alveolar proteinosis.

    abstract::A new glycoprotein with an apparent molecular weight of 130,000 was isolated, purified, and partially characterized from the pulmonary secretions which accumulate so massively in the lungs of patients suffering from pulmonary alveolar proteinosis. The amino acid analysis of the glycoprotein showed the presence of rela...


    pub_type: 杂志文章


    authors: Sahu S,Lynn WS

    更新日期:1979-09-01 00:00:00

  • Major Ozonated Autohemotherapy Preconditioning Ameliorates Kidney Ischemia-Reperfusion Injury.

    abstract::Medical ozone has therapeutic properties as an antimicrobial, anti-inflammatory, modulator of antioxidant defense system. Major ozonated autohemotherapy (MOA) is a new therapeutic approach that is widely used in the treatment of many diseases. The objective of the present study was to determine whether preischemic app...


    pub_type: 杂志文章


    authors: Sancak EB,Turkön H,Çukur S,Erimsah S,Akbas A,Gulpinar MT,Toman H,Sahin H,Uzun M

    更新日期:2016-02-01 00:00:00

  • Impact of environmental factors on efficacy of glucocorticoids in Chinese population with systemic lupus erythematosus.

    abstract::Although glucocorticoids (GCs) are effective in inducing remission in systemic lupus erythematosus (SLE) patients, there is a significant variation in response to therapeutic GCs, and some patients do not achieve full remission. The aim of this study was to explore the impact of environmental factors on the efficacy o...


    pub_type: 杂志文章


    authors: Zou YF,Xu JH,Tao JH,Xu SQ,Liu S,Chen SY,Cai J,Lian L,Chen PL,Wang DG,Liu SX,Liang CM,Ye QL,Tian G,Wu M,Pan HF,Pan FM,Su H,Ye DQ

    更新日期:2013-12-01 00:00:00

  • Inflammatory mediators as biomarkers in brain disorders.

    abstract::Neurodegenerative diseases such as Alzheimer, Parkinson, amyotrophic lateral sclerosis, and Huntington are incurable and debilitating conditions that result in progressive death of the neurons. The definite diagnosis of a neurodegenerative disorder is disadvantaged by the difficulty in obtaining biopsies and thereby t...


    pub_type: 杂志文章,评审


    authors: Nuzzo D,Picone P,Caruana L,Vasto S,Barera A,Caruso C,Di Carlo M

    更新日期:2014-06-01 00:00:00

  • Thromboxane-induced neutrophil adhesion to pulmonary microvascular and aortic endothelium is regulated by CD18.

    abstract::Thromboxane (Tx) A2 generation and subsequent selective pulmonary sequestration of neutrophils (PMNs) is characteristic of several forms of the adult respiratory distress syndrome (ARDS). Therefore, we examined PMN-dependent adhesion to cultured pulmonary microvessel and aortic endothelium (EC) in response to U46,619 ...


    pub_type: 杂志文章


    authors: Wiles ME,Welbourn R,Goldman G,Hechtman HB,Shepro D

    更新日期:1991-06-01 00:00:00

  • The Effect of Coumaric Acid on Ischemia-Reperfusion Injury of Sciatic Nerve in Rats.

    abstract::The aim of the study was to determine the effect of coumaric acid on sciatic nerve ischemia/reperfusion (SNI) injury in rats. The rats were randomly divided into four groups: control group (no medication or surgical procedure), SNI group, SNI + coumaric acid (CA) group, and SNI + methylprednisolone (MP) group. Ischemi...


    pub_type: 杂志文章


    authors: Guven M,Yuksel Y,Sehitoglu MH,Tokmak M,Aras AB,Akman T,Golge UH,Goksel F,Karavelioglu E,Cosar M

    更新日期:2015-12-01 00:00:00

  • Interleukin (IL)-10 gene polymorphisms are associated with type 2 diabetes with and without nephropathy: a study of patients from the southeast region of Iran.

    abstract::The impact of several environmental and genetic factors on diabetes and its complications is well documented. It has also been established that cytokines play a key role in the regulation of immune responses which have been shown to be important in the pathogenesis of diabetes. Studies showed that single-nucleotide po...


    pub_type: 杂志文章


    authors: Arababadi MK,Reza Mirzaei M,Ali Sajadi SM,Hassanshahi G,Ahmadabadi BN,Salehabadi VA,Derakhshan R,Kennedy D

    更新日期:2012-06-01 00:00:00

  • Immunodeficiency in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease.

    abstract::Chronic obstructive pulmonary disease (COPD), characterized by progressive airway inflammation and irreversible airflow limitation, leads to serious decline in life quality. The acute exacerbation of COPD (AECOPD) results in high healthcare costs as well as a significant mortality rate. The most common cause of acute ...


    pub_type: 杂志文章,评审


    authors: Xie S,Wang K,Zhang W,Xiao K,Yan P,Li Y,He W,Zhang Y,Xie L

    更新日期:2018-10-01 00:00:00