Oxidative status of cardinal ligament in pelvic organ prolapse.

Abstract:

:Pelvic organ prolapse (POP) is a common and distressing health problem in adult women, but the pathophysiological mechanism is yet to be fully elucidated. Previous studies have indicated that oxidative stress may be associated with POP. Thus, the aim of the present study was to investigate the oxidative status of pelvic supportive tissue in POP and further demonstrate that oxidative stress is associated with the pathogenesis of POP. A total of 60 samples were collected from females undergoing hysterectomy for POP or cervical intraepithelial neoplasia (CIN). This included 16 females with POP II, 24 females with POP III-IV (according to the POP-Q system) and 20 females with CIN II-III as the control group. Immunohistochemistry was utilized to measure the expression of oxidative biomarkers, 8-hydroxydeoxyguanosine (8-OHdG) and 4-hydroxynonenal (4-HNE). Major antioxidative enzymes, mitochondrial superoxide dismutase (MnSOD) and glutathione peroxidase 1 (GPx1) were measured through reverse transcription-quantitative polymerase chain reaction, western blotting and enzyme activity assays. The results demonstrated that in the cardinal ligament, the expression of 8-OHdG and 4-HNE was higher in the POP III-IV group compared with the POP II group and control group. The MnSOD and GPx1 protein level and enzyme activity were lower in the POP III-IV group compared with the POP II or the control group, while the mRNA expression level of MnSOD and GPx1 was increased. In conclusion, oxidative damage is increased in the pelvic supportive ligament of female patients with POP and the antioxidative defense capacity is decreased. These results support previous findings that oxidative stress is involved in the pathogenesis of POP.

journal_name

Exp Ther Med

authors

Fang G,Hong L,Liu C,Yang Q,Zhang Q,Li Y,Li B,Wu D,Wu W,Shi H

doi

10.3892/etm.2018.6633

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

3293-3302

issue

4

eissn

1792-0981

issn

1792-1015

pii

ETM-0-0-6633

journal_volume

16

pub_type

杂志文章
  • Effect of ouabain on myocardial remodeling in rats.

    abstract::The aim of this study was to investigate the effect of ouabain (EO) on myocardial remodeling. Twenty-two adult male Sprague-Dawley rats were randomly divided into two groups: the rats in the EO group (n=12) were intraperitoneally injected with EO daily and those in the control group (n=10) were injected with physiolog...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2013.1098

    authors: Ren Y,Zhang M,Zhang T,Huang R

    更新日期:2013-07-01 00:00:00

  • Early application of low-dose glucocorticoid improves acute respiratory distress syndrome: A meta-analysis of randomized controlled trials.

    abstract::Previous clinical trials have investigated the effect of glucocorticoid therapy in acute respiratory distress syndrome (ARDS), with controversial results, particularly with regard to the early administration of low dose glucocorticoid. The present meta-analysis aimed to assess whether the application of glucocorticoid...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2017.4154

    authors: Yang ZG,Lei XL,Li XL

    更新日期:2017-04-01 00:00:00

  • STAT3 speeds up progression of osteoarthritis through NF-κB signaling pathway.

    abstract::Osteoarthritis (OA) is the most common motor system disease in the elderly, with a high incidence and a huge social and economic burden. Therefore, it is urgent to study its potential pathogenesis to improve the therapeutic effect of the disease. In this study, we constructed a number of regulator-mediated OA dysfunct...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2019.8268

    authors: Wang F,Guo Z,Yuan Y

    更新日期:2020-01-01 00:00:00

  • Single-incision laparoscopically assisted vaginal hysterectomy: Operative outcomes and its learning curve.

    abstract::We previously reported on single-incision laparoscopic surgery applied to laparoscopically assisted vaginal hysterectomy (LAVH) cases. We accumulated single-incision LAVH cases to evaluate this operation, including its learning curve. Since July 2009, we planned to perform single-incision LAVH in 50 cases. Operative t...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2011.282

    authors: Koyanagi T,Motomura S

    更新日期:2011-09-01 00:00:00

  • Knockdown of lncRNA CCEPR suppresses colorectal cancer progression.

    abstract::Long non-coding RNAs (lncRNAs) serve important roles in colorectal cancer. The aim of the present study was to investigate the expression and role of cervical carcinoma expressed PCNA regulatory (CCEPR) lncRNA in colorectal cancer progression. The results demonstrated that CCEPR expression was significantly higher in ...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2019.7942

    authors: Duan Y,Fang Z,Shi Z,Zhang L

    更新日期:2019-11-01 00:00:00

  • Effects of minimally invasive plate-screw internal fixation in the treatment of posterior pelvic ring fracture.

    abstract::Effects of minimally invasive plate-screw internal fixation and sacroiliac joint screw fixation in the treatment of posterior pelvic ring fracture were compared. Continuous selection of 20 cases of unstable pelvic posterior ring fractures, according to indications of operation, were divided into a group of 13 cases of...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.6670

    authors: Li S,Meng X,Li W,Sun Z,Wang X,Qi H,Wei S,Zhou D

    更新日期:2018-11-01 00:00:00

  • 3-Hydroxy-3-methylglutaric aciduria with bilateral basal ganglia lesion: A case report.

    abstract::3-Hydroxy-3-methylglutaric aciduria (3-HMG, OMIN 246450) is a rare autosomal recessive metabolic disorder caused by a deficiency of 3-hydroxy-3-methylglutaryl-CoA lyase, a key enzyme in leucine metabolism and ketone body synthesis. Acute episodes of 3-HMG may be triggered by fasting or infection, and symptoms include ...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2016.3243

    authors: Hao X,Wang J,Liu S,Chen Y,Zhang Y,Hao Y

    更新日期:2016-06-01 00:00:00

  • Naringenin inhibits migration of lung cancer cells via the inhibition of matrix metalloproteinases-2 and -9.

    abstract::Lung cancer is among the most common causes of cancer-related mortality. It has a high mortality rate and resistance to chemotherapy due to its high metastatic potential. Naringenin, a bioactive compound identified in several fruits, displays anti-inflammatory and antitumor effects. Furthermore, naringenin mitigates t...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2016.3994

    authors: Chang HL,Chang YM,Lai SC,Chen KM,Wang KC,Chiu TT,Chang FH,Hsu LS

    更新日期:2017-02-01 00:00:00

  • MicroRNA-93 promotes cell proliferation by directly targeting P21 in osteosarcoma cells.

    abstract::MicroRNAs (miRNAs) are small, non-coding RNAs that are key regulators of gene expression by directly binding to the 3'-untranslated region of their target mRNAs, resulting in translational repression or degradation of mRNA. It has been demonstrated that miRNAs have key roles in a variety of human malignancies, includi...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2017.4204

    authors: He Y,Yu B

    更新日期:2017-05-01 00:00:00

  • Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma.

    abstract::Saw palmetto extract can induce the apoptosis of prostate cancer cells. The aim of the present study was to investigate the effect of saw palmetto extract on the phosphatidylinositol 3-kinase (PI3K)/Akt signaling transduction pathway in human glioma U87 and U251 cell lines. Suspensions of U87 and U251 cells in a logar...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2014.1756

    authors: Yang Y,Hui L,Yuqin C,Jie L,Shuai H,Tiezhu Z,Wei W

    更新日期:2014-08-01 00:00:00

  • An empirical study of modified frontolateral partial laryngectomy without tracheotomy.

    abstract::The aim of this study was to validate the feasibility of modified frontolateral partial laryngectomy without tracheotomy using animal experiments. The glottic area before and after surgery of 6 excised canine larynges and 10 canine larynges in vivo were compared to observe whether the glottic area following modified f...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2012.838

    authors: Xu H,Dong P,Sun Z,Xie J

    更新日期:2013-02-01 00:00:00

  • Expression of NUAK2 in gastric cancer tissue and its effects on the proliferation of gastric cancer cells.

    abstract::The present study aimed to analyze the expression and effects of NUAK2 in gastric cancer and adjacent normal gastric tissues. The protein expression levels of NUAK2 were detected by western blot analysis. The effects of NUAK2 expression on the proliferation of gastric cancer cells was detected using an MTT and BrdU in...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2016.3983

    authors: Tang L,Tong SJ,Zhan Z,Wang Q,Tian Y,Chen F

    更新日期:2017-02-01 00:00:00

  • Successful treatment of invasive gastric mucormycosis in a patient with alcoholic liver cirrhosis: A case report.

    abstract::Gastrointestinal (GI) mucormycosis is a rare and life-threatening invasive fungal infection. GI mucormycosis occur in all parts of the alimentary tract, with the stomach being the most common site. Diabetes mellitus and other types of conditions associated with immunodeficiency, including hematologic malignancies, sol...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2014.1753

    authors: Lee SH,Son YG,Sohn SS,Ryu SW

    更新日期:2014-08-01 00:00:00

  • CTRP3 induces an intermediate switch of CD14++CD16+ monocyte subset with anti-inflammatory phenotype.

    abstract::Acute myocardial infarction (AMI) evokes a temporally coordinated immune response, in which monocytes are critically involved in the clearance of cell debris; however, excessive inflammation induced by the classical sub-population of monocytes frequently limits the endogenous reparative process. In the present study, ...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2020.8467

    authors: Zhu H,Ding Y,Zhang Y,Ding X,Zhao J,Ouyang W,Gong J,Zou Y,Liu X,Wu W

    更新日期:2020-03-01 00:00:00

  • Treatment of recurrent patellar dislocation via knee arthroscopy combined with C-arm fluoroscopy and reconstruction of the medial patellofemoral ligament.

    abstract::Recurrent patellar dislocations were treated via knee arthroscopy combined with C-arm fluoroscopy, and reconstruction of the medial patellofemoral ligaments. Between October 2013 and March 2017, 52 cases of recurrent patellar dislocation [27 males and 25 females; age, 16-47 years (mean, 21.90 years)] were treated. Art...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.6055

    authors: Li L,Wang H,He Y,Si Y,Zhou H,Wang X

    更新日期:2018-06-01 00:00:00

  • Purulent bullous epidermal necrolysis: A potential new clinical pattern of drug eruption.

    abstract::Drug eruption is a major problem of adverse drug reactions and may present as variform clinical manifestations. Toxic epidermal necrolysis (TEN) and acute generalized exanthematous pustulosis (AGEP) are relatively rare severe drug eruptions. It has rarely been reported that AGEP overlaps or mimics TEN, while no purule...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2013.944

    authors: Liu LF,Yan JL,Wang JY,Chen JS,Cai SQ,Lao LM,Zheng M

    更新日期:2013-04-01 00:00:00

  • Patient outcome prediction using multiple biomarkers in human melanoma: A clinicopathological study of 118 cases.

    abstract::The application of biomarkers in melanoma prognosis has been well recognized. However the ability of a single biomarker to predict melanoma patient outcome is usually limited. We previously examined the expression of ten biomarkers (Bim, BRG1, BRMS1, CTHRC1, ING4, NQO1, NF-κB-p50, PUMA, SNF5 and SOX4) in melanomas. To...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2010.169

    authors: Li J,Zhang Z,Li G

    更新日期:2011-01-01 00:00:00

  • Resolvin E1 reduces hepatic fibrosis in mice with Schistosoma japonicum infection.

    abstract::The aim of this study was to investigate whether resolvin E1 (RvE1) protects against hepatic fibrosis in a murine model of liver fibrosis induced by Schistosoma japonicum infection. A total of 30 pathogen-free Kunming mice were randomly and equally divided into three groups: Control (uninfected, untreated), model (inf...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2014.1641

    authors: Qiu W,Guo K,Yi L,Gong Y,Huang L,Zhong W

    更新日期:2014-06-01 00:00:00

  • Continuous administration of recombinant human endostatin (Endostar): A pre-clinical safety study.

    abstract::The aim of this study was to evaluate the safety of the continuous administration of recombinant human endostatin (Endostar) in healthy mice. A total of 16 nude mice were randomly divided into four treatment groups: a continuous administration group injected intraperitoneally (i.p.) with 14 mg/kg Endostar over seven d...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2012.534

    authors: Huang R,Zhan Q,Zhou X,Chu Z,Jiang J,Liang X

    更新日期:2012-06-01 00:00:00

  • Predictive factors of pancreatic necrosectomy following percutaneous catheter drainage as a primary treatment of patients with infected necrotizing pancreatitis.

    abstract::Pancreatic necrosectomy (PN) following percutaneous catheter drainage (PCD) is an effective method of treating patients with necrotizing pancreatitis, however, the predictive factors for PN after PCD have not yet been identified. A total of 74 patients with suspected infected necrotizing pancreatitis (INP) and peripan...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2017.5107

    authors: Cao X,Cao F,Li A,Gao X,Wang XH,Liu DG,Fang Y,Guo DH,Li F

    更新日期:2017-11-01 00:00:00

  • Surgical treatment for hydrosalpinx increases the expression of integrin αvβ3 in the endometrium during the implantation window.

    abstract::To investigate the clinical importance of increased integrin αvβ3 expression in the endometrium following the surgical treatment for hydrosalpinx, a total of 60 patients with hydrosalpinx and 30 patients with fallopian tube obstruction were recruited. In the implantation window, immunohistochemistry was performed to d...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2012.610

    authors: Zhong Y,Li J,Wu H,Ying Y,Liu Y,Zhou C,Xu Y,Shen X,Qi Q

    更新日期:2012-09-01 00:00:00

  • Significance of dynamic evolution of TNF-α, IL-6 and intestinal fatty acid-binding protein levels in neonatal necrotizing enterocolitis.

    abstract::To study the significance of dynamic evolution of serum tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6) and intestinal fatty acid-binding protein (I-FABP) levels in neonatal necrotizing enterocolitis (NEC). A total of 45 NEC child patients, 45 non-NEC child patients and 45 healthy newborns were enrolled. After t...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2017.5532

    authors: Li Z,Sheng L

    更新日期:2018-02-01 00:00:00

  • Association between inflammatory cytokines and the risk of post-stroke depression, and the effect of depression on outcomes of patients with ischemic stroke in a 2-year prospective study.

    abstract::The association between inflammatory cytokines and the risk of post-stroke depression (PSD) remains unclear. The aim of the present study was to investigate this association and the effect of PSD on the outcomes of ischemic stroke patients. A total of 355 patients who had experienced ischemic stroke participated in in...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2016.3494

    authors: Jiao JT,Cheng C,Ma YJ,Huang J,Dai MC,Jiang C,Wang C,Shao JF

    更新日期:2016-09-01 00:00:00

  • Association of epidermal growth factor receptor and K-Ras mutations with smoking history in non-small cell lung cancer patients.

    abstract::Lung cancer, a major health problem affecting the epithelial lining of the lower respiratory tract, is considered to be one of the deadliest types of cancer in males and females and it is well-known that smoking is the chief cause of lung cancer. In addition to smoking and environmental factors, genetic susceptibility...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2012.829

    authors: Baykara O,Tansarikaya M,Demirkaya A,Kaynak K,Tanju S,Toker A,Buyru N

    更新日期:2013-02-01 00:00:00

  • Matrix metalloproteinase 14 participates in corneal lymphangiogenesis through the VEGF-C/VEGFR-3 signaling pathway.

    abstract::The aim of the present study was to investigate the roles of matrix metalloproteinase 14 (MMP-14) in corneal inflammatory lymphangiogenesis. The expression of MMP-14 in vivo was detected by immunohistochemistry, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot assays, under vario...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2016.3601

    authors: Du HT,Liu P

    更新日期:2016-10-01 00:00:00

  • Exenatide reduces oxidative stress and cell death in testis in iron overload rat model.

    abstract::Glucagon-like peptide-1 (GLP-1) has been demonstrated to affect the oxidative stress status in several in vitro, in vivo and clinical studies. The aim of the present study was to evaluate the effect of a GLP-1 analogue, exenatide, on oxidative stress parameters and apoptotic markers in testicular cells in an iron over...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.6795

    authors: Yesil S,Sungu N,Kilicarslan A,Kuskonmaz SM,Kara H,Kucuk A,Polat F,Kavutcu M,Arslan M

    更新日期:2018-12-01 00:00:00

  • Acetylpuerarin inhibits oxygen-glucose deprivation-induced neuroinflammation of rat primary astrocytes via the suppression of HIF-1 signaling.

    abstract::In the central nervous system (CNS), ischemic injury induced by inflammation associated with astrocytes serves an important role in physiological and pathological processes. Neuroinflammation leads to the release of pro-inflammatory cytokines, including tumor necrosis factor-α and interleukin-1β. The aim of the presen...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2018.6509

    authors: Xiang Y,Du P,Zhang X,Biswas S,Jiao G,Liu H

    更新日期:2018-09-01 00:00:00

  • A novel CpG-based signature for survival prediction of lung adenocarcinoma patients.

    abstract::Lung adenocarcinoma (LACA) is the leading cause of cancer-associated death worldwide. The present study intended to identify DNA methylation patterns that may serve as diagnostic and prognostic biomarkers for LACA. Data on DNA methylation and the survival data of the patients of LACA were obtained from The Cancer Geno...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2019.8200

    authors: Zheng R,Xu H,Mao W,Du Z,Wang M,Hu M,Gu X

    更新日期:2020-01-01 00:00:00

  • A retrospective analysis of the risk factors associated with systemic air embolism following percutaneous lung biopsy.

    abstract::In the present study, the risk factors for systemic air embolism as a complication of percutaneous CT-guided lung biopsy were explored. Data from 2,026 percutaneous CT-guided lung biopsy procedures were retrospectively analyzed. All cases were divided into a concurrent air embolism group and a control group, depending...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2019.8208

    authors: Liu SH,Fu Q,Yu HL,Yang Q,Hu YB,Zhang ZX,Zhang BP,Zhang CY

    更新日期:2020-01-01 00:00:00

  • Gilbert syndrome with systemic lupus erythematosus presenting with persistent unconjugated hyperbilirubinemia: A case report.

    abstract::Gilbert syndrome (GS) is a hereditary unconjugated hyperbilirubinemia that results from mutations in the bilirubin uridine diphosphate-glucuronosyltransferase (UGT1A1) gene. To the best of our knowledge, there are currently no reports that focus on patients with systemic lupus erythematosus (SLE) coexisting with GS. T...

    journal_title:Experimental and therapeutic medicine

    pub_type: 杂志文章

    doi:10.3892/etm.2020.9219

    authors: Ye N,Zhou Z,Gong H,Teng J,Han Y,Yang C,Ye J

    更新日期:2020-11-01 00:00:00