Abstract：
Aims:To explore the biological roles of alpha-fetoprotein (AFP), a tumor-associated antigen in human hepatocellular carcinoma (HCC). Materials and Methods:After knockdown of AFP in HepG2 cells by transfection of specific Stealth™ RNAi, the expression of AFP were detected by reverse transcription polymerase chain reaction at mRNA level and by enzyme-linked immunosorbent assay at the protein level. Then, the effect of silenced AFP on cell proliferation was assessed by dimethylthiazolyl-2,5-diphenyl-tetrazolium bromide assay, and apoptosis assessment with Hoechst33258 and flow cytometry (double stain with fluorescein isothiocyanate/propidium iodide), the roles of AFP in the cell cycle regulation were assessed by flow cytometry. We also detected the expression of some key proteins related to apoptosis pathway by Western immunoblot analysis. Results:After the transfection for 48 h, the expression of AFP gene was almost abolished, the cell proliferation was inhibited by 47.61%, the number of cells undergoing early apoptosis was significantly increased to 59.47%; cell cycle was arrested with the increase of G0/G1 phase cells from 45.3% to 58.4%. Inhibition of AFP expression also results in decreasing of transforming growth factor-β (TGF-β), mutant P53 expression, and increasing of Bax/Bcl-2 ratio, activation of caspase-3. Conclusions:The results suggest that AFP may positively regulate cell proliferation by enhancing the apoptosis resistance via effect on TGF-β and p53/Bax/caspase-3 signaling pathway in HepG2 cells. As such, the knockdown of AFP gene should be further investigated in vivo as a novel approach to HCC treatment.
journal_name
J Cancer Res Therjournal_title
Journal of cancer research and therapeuticsauthors
Yang X,Chen L,Liang Y,Si R,Jiang Z,Ma B,Gao Pdoi
10.4103/0973-1482.180681subject
Has Abstractpub_date
2018-09-01 00:00:00pages
S634-S643issue
Supplementeissn
0973-1482issn
1998-4138pii
JCanResTher_2018_14_10_634_180681journal_volume
14pub_type
杂志文章abstract：Objective:In this study, we showed a modified method for the isolation of cancer stem cells (CSCs) using a combination of differential adhesion method and serum-free culture medium (SFM) method. Materials and Methods:Trypsin-sensitive cells and trypsin-resistant cells were isolated from MB49, EJ, and SK-OV-3 cells usi...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.174533
更新日期：2018-06-01 00:00:00
abstract：:The present case report defines a rare case of a liposarcoma with bone metastasis resulting in a complete remission (CR) following trabectedin treatment. The patient was referred with abdominal swelling and pain. A retroperitoneal mass was detected and described as dedifferentiated liposarcoma (DDLS). The mass was sur...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件,评审
doi：10.4103/0973-1482.158032
更新日期：2015-10-01 00:00:00
abstract：AIMS:Hereditary nonpolyposis colorectal cancer (HNPCC) is a subgroup of colorectal cancer (CRC) which should be differentiated because of the high risk for additional cancers and risk evaluation for other family members, especially for CRC. It is not practical to perform genetic testing for all CRC patients; therefore,...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.174558
更新日期：2017-04-01 00:00:00
abstract：:Masseter hypertrophy is a characteristic condition resulting from an increase in the size of the muscle mass. At times, it produces significant facial asymmetry and is an important finding in the differential diagnosis of similar located entities. A proper diagnosis of this condition would avoid more aggressive and un...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件
doi：10.4103/0973-1482.204889
更新日期：2018-12-01 00:00:00
abstract：:Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the gastrointestinal tract. Tyrosine kinase inhibitors (TKIs), such as imatinibmesylate, have been used for the adjuvant treatment of KIT-positive GISTs. Several pathologic changes after imatinib treatment of metastatic GIST including tumor ...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件,评审
doi：10.4103/0973-1482.136030
更新日期：2014-07-01 00:00:00
abstract：Aim of Study:The conclusions on the association between glutathione S-transferase P1 (GSTP1) gene polymorphism and lung cancer risk are still debated. This meta-analysis was performed to update the association between GSTP1 and the risk of lung cancer. Materials and Methods:The association investigations were identifi...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章,meta分析
doi：10.4103/0973-1482.199455
更新日期：2018-12-01 00:00:00
abstract：Purpose:We aimed to report the experience of intraoperative electron radiation therapy (IOERT) with Mobetron (Intraop Medical Incorporated, Santa Clara, CA, USA) as a partial breast irradiation (PBI) for patients with early-stage breast cancer and explanation of IOERT application and present early clinical and cosmetic...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_191_17
更新日期：2019-07-01 00:00:00
abstract：Objective:The aim of this study is to investigate the application and the feasibility of microwave ablation in laparoscopic partial splenectomy. Materials and Methods:From January 2018 to June 2019, four patients with benign spleen lesions in our hospital underwent laparoscopic partial splenectomy assisted by microwav...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_816_19
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVE:The objective of this study was to compare robotic-prostatectomy plus adjuvant radiation therapy (RPRAT) versus primary RT for high-risk prostate cancer (HRPCa). MATERIALS AND METHODS:A retrospective chart review was performed for the HRPCa patients treated in our institution between 2000 and 2010. One hundr...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.139601
更新日期：2015-01-01 00:00:00
abstract：Background:Baicalein is an active compound extracted from the roots of Scutellaria baicalensis georgi, which is widely and traditionally used in the anticancer therapy. Notch signaling pathway is usually abnormally activated in kinds of human cancers. The aim of the present study is to investigate the antitumor effects...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.204899
更新日期：2019-10-01 00:00:00
abstract：:Cutaneous metastasis from transitional cell bladder carcinoma is a rare clinical entity associated with poor prognosis. We present a case of cutaneous metastasis arising from a transitional cell bladder carcinoma in a male patient who had undergone a radical cystectomy and bilateral ureterostomy 17 months previously. ...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章,评审
doi：10.4103/0973-1482.82940
更新日期：2011-04-01 00:00:00
abstract：:Melanoma is rare in children, but its incidence appears to be increasing. Melanoma accounts for the highest mortality among all skin cancer types. This disease is characterized by high-grade malignancy, easy metastasis, poor prognosis, and high death rate. Melanoma in children may be biologically different from that i...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章,评审
doi：10.4103/0973-1482.145788
更新日期：2014-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the value of two quantitative indicators, the apparent diffusion coefficient (ADC) and the exponent apparent diffusion coefficient (EADC), of magnetic resonance imaging (MRI) diffusion-weighted imaging (DWI) in the differential diagnosis of ovarian epithelial tumors. ...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.163667
更新日期：2016-01-01 00:00:00
abstract：Objective:This study aimed to explore the risk factors of hepatocellular carcinoma (HCC) and survival analysis for cirrhosis after transjugular intrahepatic portosystemic shunt (TIPS) in treating portal hypertension. Materials and Methods:A retrospective database review was performed, including 106 patients (33 female...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_930_17
更新日期：2018-01-01 00:00:00
abstract：PURPOSE:To report our experience of stereotactic radiosurgery (SRS) in consecutively treated patients with arteriovenous malformations (AVMs). MATERIALS AND METHODS:Of the 87 patients, 23 patients qualified and were treated with SRS as per predefined protocol according to AVM size, location, neurological status, prior...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.57124
更新日期：2009-07-01 00:00:00
abstract：:Intra-nodal palisaded myofibroblastoma (IPM) is a rare benign lymph node mesenchymal tumor. It presents as a slow growing, painless nodular mass confined mostly to the inguinal area. Histologically, it shows palisading spindle cells, hemorrhages, hemosiderin laden macrophages, and amianthoid fibers, almost totally rep...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.113395
更新日期：2013-04-01 00:00:00
abstract：:Carcinoma of the lip is a slow-growing locally-advanced disease with low metastatic potential. Distant skeletal metastasis is reported very rarely and the vertebral column is the most common site. A 58-year-old male smoker presented with pain and massive swelling of the left scapula for six months. He also noticed a s...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.73338
更新日期：2010-07-01 00:00:00
abstract：CONTEXT:Biopsy Gleason score (b-GS) is often different from the Gleason score obtained after analysis of radical prostatectomy (RP) specimen (rp-GS). Upgradation has an important implication in decision making for cancer prostate management, and is the focus of this study. AIM:To evaluate Gleason score upgradation aft...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.92021
更新日期：2011-10-01 00:00:00
abstract：:Mucoepidermoid carcinomas (MEC) are rare malignant tumors that originate in the submucosal bronchial glands, and complete resection usually correlates with favorable prognosis. A 54-year-old male patient was diagnosed with high-grade MEC in the left main bronchus via bronchoscopy. After the patient was diagnosed with ...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件
doi：10.4103/0973-1482.136665
更新日期：2014-04-01 00:00:00
abstract：:Placement of long term central venous access devices (CVAD) such as chemo ports and Hickman's catheters are associated with a definite risk of catheter tip malpositioning. As such, malpositioning runs a risk of venous thrombosis and related complications; it is imperative to reposition the catheter. Percutaneous trans...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.63554
更新日期：2010-01-01 00:00:00
abstract：:A rare and unique variant of breast cancer--metaplastic matrix producing carcinoma, is presented. Surgery has been the mainstay of treatment. The role of adjuvant postoperative radiotherapy, as has been used in this patient, has been reviewed. ...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.25855
更新日期：2006-04-01 00:00:00
abstract：:The aim of this work is to develop a wireless local area network (LAN) between different types of users (Radiation Oncologists, Radiological Physicists, Radiation Technologists, etc) for efficient patient data management and to made easy the availability of information (chair side) to improve the quality of patient ca...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.65236
更新日期：2010-04-01 00:00:00
abstract：:In recent years, using the ionizing radiation in the interventional cardiology has been increased; this is because of the rapid growth of the number of interventional procedures and the high levels of radiation dose in these examinations. Therefore, it is necessary to develop procedures for managing the use of fluoros...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章,评审
doi：10.4103/0973-1482.192767
更新日期：2017-10-01 00:00:00
abstract：PURPOSE:This study was designed to evaluate inter and intraobserver concordance in gross tumor volume (GTV) delineation on megavoltage CT (MVCT) images in patients with postoperative vault recurrences. MATERIALS AND METHODS:Three observers delineated GTV on MVCT and CECT and two observers recontoured on MVCT images. T...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.87022
更新日期：2011-07-01 00:00:00
abstract：:Large pedunculated esophageal lipoma is uncommon. The presenting symptoms of esophageal lipoma are dysphagia, regurgitated mass, and persistent sensation of a lump in the throat. The most frequent location of the tumor pedicle is the upper esophageal sphincter. Here, we present the case of a 52-year-old man who had th...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件,评审
doi：10.4103/0973-1482.154089
更新日期：2015-10-01 00:00:00
abstract：BACKGROUND:Telomeres, which are bound with shelterin protein complex, play an important role in maintaining genomic stability and its dysfunction may lead to carcinogenesis. Here, we aimed to analyze whether shelterin complex gene expression and telomere length variation, play any role in gallbladder carcinogenesis. M...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/0973-1482.184512
更新日期：2017-04-01 00:00:00
abstract：Background:We assessed the frequency of epigenetic lesions, including lymphoid-specific helicase (LSH), 5-hydroxymethylcytosine (5-hmC) and E2F1, and the possible correlations among molecular findings, phenotype, clinical features, and outcome. Methods:We investigated 181 paraffin-embedded B-cell lymphoma samples usin...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_243_18
更新日期：2019-01-01 00:00:00
abstract：:Lipoblastoma and lipoblastomatosis are benign adipocytic tumors seen in children less than 3 years of age. Awareness amongst surgeons of this fast growing lipomatous tumor in young children is important to avoid mutilating surgery and chemotherapy. Histological diagnosis of lipoblastoma is sometimes difficult because ...
journal_title：Journal of cancer research and therapeutics
pub_type： 信件
doi：10.4103/0973-1482.176135
更新日期：2015-10-01 00:00:00
abstract：Background:Apatinib has been approved for the treatment of advanced gastric adenocarcinoma and gastric-esophageal junctional adenocarcinoma, but its efficacy is unknown for other advanced solid tumors. Aims and Objectives:We retrospectively reviewed the use of apatinib for multiple advanced-stage non-gastric cancers. ...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_24_19
更新日期：2019-01-01 00:00:00
abstract：Background:Intractable ascites secondary to malignant disease deteriorates patients' quality of life. The purpose of this study was to evaluate the safety and efficacy of percutaneous peritoneovenous (Denver) shunt in treating intractable malignant ascites in cancer patients. Materials and Methods:Thirty-five patients...
journal_title：Journal of cancer research and therapeutics
pub_type： 杂志文章
doi：10.4103/jcrt.JCRT_606_18
更新日期：2020-12-01 00:00:00
