Knockdown of alpha-fetoprotein expression inhibits HepG2 cell growth and induces apoptosis.


Aims:To explore the biological roles of alpha-fetoprotein (AFP), a tumor-associated antigen in human hepatocellular carcinoma (HCC). Materials and Methods:After knockdown of AFP in HepG2 cells by transfection of specific Stealth™ RNAi, the expression of AFP were detected by reverse transcription polymerase chain reaction at mRNA level and by enzyme-linked immunosorbent assay at the protein level. Then, the effect of silenced AFP on cell proliferation was assessed by dimethylthiazolyl-2,5-diphenyl-tetrazolium bromide assay, and apoptosis assessment with Hoechst33258 and flow cytometry (double stain with fluorescein isothiocyanate/propidium iodide), the roles of AFP in the cell cycle regulation were assessed by flow cytometry. We also detected the expression of some key proteins related to apoptosis pathway by Western immunoblot analysis. Results:After the transfection for 48 h, the expression of AFP gene was almost abolished, the cell proliferation was inhibited by 47.61%, the number of cells undergoing early apoptosis was significantly increased to 59.47%; cell cycle was arrested with the increase of G0/G1 phase cells from 45.3% to 58.4%. Inhibition of AFP expression also results in decreasing of transforming growth factor-β (TGF-β), mutant P53 expression, and increasing of Bax/Bcl-2 ratio, activation of caspase-3. Conclusions:The results suggest that AFP may positively regulate cell proliferation by enhancing the apoptosis resistance via effect on TGF-β and p53/Bax/caspase-3 signaling pathway in HepG2 cells. As such, the knockdown of AFP gene should be further investigated in vivo as a novel approach to HCC treatment.


J Cancer Res Ther


Yang X,Chen L,Liang Y,Si R,Jiang Z,Ma B,Gao P




Has Abstract


2018-09-01 00:00:00














  • Chondrosarcoma of the distal phalanx of the right great toe: report of a rare malignancy and review of literature.

    abstract::Foot is an uncommon site for chondrosarcoma and involvement of phalanges is extremely rare. Here, a case of low-grade chondrosarcoma of the distal phalanx of the right great toe in a 37-year-old male patient is being reported. The patient complained of gradual swelling of the right great toe and pain for last 2 years....

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章,评审


    authors: Mondal SK

    更新日期:2012-01-01 00:00:00

  • Association of clinicopathological features with E-cadherin (CDH1) gene-160 C>A promoter polymorphism in Turkish colorectal cancer patients.

    abstract:Background and Aim of Study:The role of E-cadherin (CDH1) gene-160 C>A (rs16260) promoter polymorphism in colorectal cancer (CRC) still remains inconclusive. The aim of this study is to investigate the associations between the CDH1-160 C>A polymorphism with the susceptibility and clinicopathological development of CRC ...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Bahadir A,Eral G,Budak M,Shimamoto F,Korpinar MA,Erdamar S,Tuncel H

    更新日期:2019-01-01 00:00:00

  • The overexpression of MRP4 is related to multidrug resistance in osteosarcoma cells.

    abstract::Doxorubicin (Adriamycin, ADM) is an antimitotic drug used in the treatment of a wide range of malignant tumors, including acute leukemia, lymphoma, osteosarcoma, breast cancer, and lung cancer. Multidrug resistance-associated proteins (MRPs) are members of a superfamily of ATP-binding cassette (ABC) transporters, whic...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: He Z,Hu B,Tang L,Zheng S,Sun Y,Sheng Z,Yao Y,Lin F

    更新日期:2015-01-01 00:00:00

  • Feasibility and safety of GliaSite brachytherapy in treatment of CNS tumors following neurosurgical resection.

    abstract:PURPOSE:To investigate feasibility and safety of GliaSite brachytherapy for treatment of central nervous system (CNS) tumors following neurosurgical resection. We report mature results of long-term follow-up, outcomes and toxicity. MATERIALS AND METHODS:In the period from 2004 to 2007, 10 consecutive adult patients wi...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 临床试验,杂志文章


    authors: Wernicke AG,Sherr DL,Schwartz TH,Pannullo SC,Stieg PE,Boockvar JA,Ivanidze J,Moliterno JA,Parashar B,Trichter S,Sabbas AM,Nori D

    更新日期:2010-01-01 00:00:00

  • Knowledge, awareness, and practice of breast self-examination among females in Mosul city, Iraq.

    abstract:Aims:This study is carried out to report on the knowledge and practice regarding breast self-examination (BSE) among women from the city of Mosul in Iraq and to evaluate the prevalence of performing clinical breast examination (CBE) and mammography among them. Settings and Design:A descriptive, cross-sectional survey ...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Al-Qazaz HK,Yahya NA,Ibrahim DK

    更新日期:2020-10-01 00:00:00

  • Serum paraoxonase and arylesterase can be useful markers to predict neoadjuvant chemotherapy requirement in patients with breast cancer.

    abstract:Aim:The aims of this study are to evaluate the serum levels of paraoxonase (PON) and arylesterase (ARE) in breast cancer (BC) patients; to determine their relationship with chemotherapy requirements in BC; and to find a cut-off value to assess subjects with a higher risk of BC. Subjects and Methods:A total of 40 BC pa...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Okuturlar Y,Gunaldi M,Kocoglu H,Hursitoglu M,Gedikbasi A,Acarer D,Harmankaya O,Kumbasar A

    更新日期:2018-06-01 00:00:00

  • Unusual clinical and radiological presentation of metastatic choriocarcinoma to the brain and long-term remission following emergency craniotomy and adjuvant EMA-CO chemotherapy.

    abstract::Choriocarcinoma is the most malignant tumor of gestational trophoblast origin. Metastasis to brain is considered a poor prognostic indicator. Recent advances in adjuvant radiotherapy and chemotherapy have led to an excellent outcome of these patients. Craniotomy is indicated in selected cases with cerebral metastases....

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Dadlani R,Furtado SV,Ghosal N,Prasanna KV,Hegde AS

    更新日期:2010-10-01 00:00:00

  • Radiation recall phenomenon presenting as myositis triggered by carboplatin plus paclitaxel and related literature review.

    abstract::While most case reports to date are radiation recall dermatitis, radiation recall myositis, which is a distinct form of radiation recall phenomenon caused by carboplatin plus paclitaxel, has not been reported. We treated a 57-year-old female patient who suffered from recurrent cervical cancer. When the patient develop...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 信件,评审


    authors: Maeng CH,Park JS,Lee SA,Kim DH,Yun DH,Yoo SD,Kim HS,Chon J

    更新日期:2014-10-01 00:00:00

  • To B(iopsy) or not to B(iopsy).

    abstract::Often in metastatic disease, biopsy confirmation of suspicious central nervous system (CNS) lesions is not mandated according to the American College of Radiology, International Radiosurgery Association, and the National Comprehensive Cancer Network. We present a case of an individual who was thought to have metastati...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 信件


    authors: Gestaut MM,Strober ED,Jhavar SG,Patel MK

    更新日期:2017-10-01 00:00:00

  • Frequency of epidermal growth factor receptor mutations in Jordanian lung adenocarcinoma patients at diagnosis.

    abstract:BACKGROUND:Somatic mutations of the epidermal growth factor receptor (EGFR) gene have been associated with tumor response to tyrosine kinase inhibitors (TKIs) and favorable outcome in patients with non-small-cell lung cancer (NSCLC). The activating mutations that confer sensitivity to EGFR TKIs are present in the TK do...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Obeidat N,Awidi A,Ababneh N,Shomaf M,Al-Adaily T,Jaber M,Al-Khateeb M,Abbasi S

    更新日期:2016-04-01 00:00:00

  • Favorable progression-free survival in women with two different histopathological subtypes of bilateral breast cancer.

    abstract:Purpose:The aim of this study was to classify bilateral breast cancers (BBCs) into two groups according to histopathological or molecular subtypes of the two breast diseases in each patient and to study their characteristics in relation to survival outcomes. Methods:Fifty-six BBC patients were enrolled in the study. T...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Wang Y,Du C,Tu L,Luo F,Yan X

    更新日期:2018-01-01 00:00:00

  • Rechallenge of camrelizumab in non-small-cell lung cancer patients treated previously with camrelizumab and microwave ablation.

    abstract::Camrelizumab is a programmed death receptor-1 inhibitor originally developed in China for the treatment of refractory lymphoma. It has also been effective in non-small-cell lung cancer patients. However, the rechallenge of camrelizumab was not reported previously. We report the rechallenge of camrelizumab therapy in t...

    journal_title:Journal of cancer research and therapeutics



    authors: Wei Z,Yang X,Ye X

    更新日期:2020-09-01 00:00:00

  • Low health literacy, poor knowledge, and practice among Turkish women patients undergoing cervical cancer screening.

    abstract:Objective:The objective of this study is to evaluate health literacy level, cervical cancer knowledge, and practice among women patients undergoing screening. Materials and Methods:A cross-sectional study was conducted among 455 women family health center patients. Health literacy level was evaluated by Rapid Estimate...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Yilmazel G

    更新日期:2019-10-01 00:00:00

  • Comparative study of the anatomic segmentectomy versus lobectomy for clinical stage IA peripheral lung cancer by video assistant thoracoscopic surgery.

    abstract:OBJECTIVE:The objective of this study was to compare the completely thoracoscopic anatomic segmentectomy with lobectomy to treat stage I(A) peripheral lung cancer <2 cm. MATERIALS AND METHODS:A retrospective study was performed that 54 cases stage I(A) peripheral lung cancer patients were selected, including 26 cases ...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Zhang L,Ma W,Li Y,Jiang Y,Ma G,Wang G

    更新日期:2013-09-01 00:00:00

  • Clinical significance of Twist, E-cadherin, and N-cadherin protein expression in endometrioid adenocarcinoma.

    abstract:OBJECTIVE:The aim of the study was to investigate the expression of Twist, E-cadherin, and N-cadherin both in normal endometrium and in endometrioid adenocarcinoma tissues (NET and EAT), and further discuss the relationship between the proteins expression and the clinical parameters. METHODS:Seventy-six EAT and 50 NET...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Xie X,Zheng X,Wang J,Chen L

    更新日期:2017-01-01 00:00:00

  • An overview on small-field dosimetry in photon beam radiotherapy: Developments and challenges.

    abstract::Small fields are more repeatedly used for radiation therapy as small segments in intensity-modulated radiotherapy or as in the form of independent fields in stereotactic radiosurgery and other novel equipment such as cyberknife and tomotherapy. Nevertheless, the application of small fields for radiotherapy of lung mak...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章,评审


    authors: Bagheri H,Soleimani A,Gharehaghaji N,Mesbahi A,Manouchehri F,Shekarchi B,Dormanesh B,Dadgar HA

    更新日期:2017-04-01 00:00:00

  • Telomere length variation and expression analysis of shelterin complex genes during gallbladder carcinogenesis.

    abstract:BACKGROUND:Telomeres, which are bound with shelterin protein complex, play an important role in maintaining genomic stability and its dysfunction may lead to carcinogenesis. Here, we aimed to analyze whether shelterin complex gene expression and telomere length variation, play any role in gallbladder carcinogenesis. M...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Poojary SS,Mishra G,Singh TD,Gupta S,Shrivastav BR,Tiwari PK

    更新日期:2017-04-01 00:00:00

  • Effect of gemcitabine on the uptake of (18)F-fluorodeoxyglucose and (18)F-fluorothymidine in lung adenocarcinoma A549 cells and the animal tumor model.

    abstract:BACKGROUND:Gemcitabine is the first-line drug for nonsmall cell lung cancer, and 18F-fluorodeoxyglucose. (18F-FDG) and 18F-fluorothymidine. (18F-FLT) are positron emission tomography. (PET) imaging agents. The aim of this study was to explore the effect of gemcitabine on the uptake of 18F-FDG and 18F-FLT in A549 cells ...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Zhang B,Deng SM,Guo LC,Dong JJ,Zhu YB,Gao Y,Wang ZX,Cho WC

    更新日期:2016-01-01 00:00:00

  • An updated meta-analysis for association of glutathione S-transferase P1 gene polymorphism with the susceptibility of lung cancer.

    abstract:Aim of Study:The conclusions on the association between glutathione S-transferase P1 (GSTP1) gene polymorphism and lung cancer risk are still debated. This meta-analysis was performed to update the association between GSTP1 and the risk of lung cancer. Materials and Methods:The association investigations were identifi...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章,meta分析


    authors: Liu WZ,Sun Y,Feng X,Bi XH,Liu T,Zhou HF

    更新日期:2018-12-01 00:00:00

  • Intraoperative radiotherapy with electrons as partial breast irradiation in limited stage breast cancer: Early term clinical and cosmetic outcomes.

    abstract:Purpose:We aimed to report the experience of intraoperative electron radiation therapy (IOERT) with Mobetron (Intraop Medical Incorporated, Santa Clara, CA, USA) as a partial breast irradiation (PBI) for patients with early-stage breast cancer and explanation of IOERT application and present early clinical and cosmetic...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Avci GG,Güney Y,Küçükpilakci B,Çalikoğlu T,Gülçelik MA,Özaslan C

    更新日期:2019-07-01 00:00:00

  • Complete response of a recurrent-metastatic liposarcoma with dedifferentiated histological features following the administration of trabectedin and review of literature.

    abstract::The present case report defines a rare case of a liposarcoma with bone metastasis resulting in a complete remission (CR) following trabectedin treatment. The patient was referred with abdominal swelling and pain. A retroperitoneal mass was detected and described as dedifferentiated liposarcoma (DDLS). The mass was sur...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 信件,评审


    authors: Kus T,Aktas G,Kalender ME,Tutar E,Ulker E,Camci C

    更新日期:2015-10-01 00:00:00

  • Adding bit of esthetics with science modification of batwing mastopexy for breast conservation in a young patient with giant fibroadenoma breast.

    abstract::A 19-year-old girl presented with a lump in her right breast and with a history of surgery for the similar complaint 3 years back. Ultrasound was suggestive of benign solitary lesion of size 16 cm × 10 cm. Core biopsy was suggestive of phyllodes tumor, and the histopathology report of previous surgery was also suggest...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 信件


    authors: Kumar HH,Rahman NKF,Ramesh RS,Raghunandan GC

    更新日期:2019-07-01 00:00:00

  • Evaluation of anaplastic lymphoma kinase expression in nonsmall cell lung cancer;a tissue microarray analysis.

    abstract:INTRODUCTION:The oncogenic form of anaplastic lymphoma kinase (ALK) gene is an attractive candidate marker for diagnostic and therapeutic purposes in several malignancies, including nonsmall cell lung cancer (NSCLC). This study aimed to examine the expression levels and clinical significance of ALK in a series of NSCLC...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Roudi R,Haji G,Madjd Z,Shariftabrizi A,Mehrazma M

    更新日期:2016-04-01 00:00:00

  • Dosimetric verification of dose calculation algorithm in the lung during total marrow irradiation using helical tomotherapy.

    abstract:INTRODUCTION:Treatment of proliferative diseases of the hematopoietic system involves, in most cases, chemotherapy combined with radiation therapy, which is intended to provide adequate immunosuppressant. Conventionally, total body irradiation (TBI) was used; however, total marrow irradiation (TMI) performed with helic...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Konstanty E,Malicki J,Łagodowska K,Kowalik A

    更新日期:2017-01-01 00:00:00

  • A rarely seen mucoepidermoid carcinoma of the left main bronchus.

    abstract::Mucoepidermoid carcinomas (MEC) are rare malignant tumors that originate in the submucosal bronchial glands, and complete resection usually correlates with favorable prognosis. A 54-year-old male patient was diagnosed with high-grade MEC in the left main bronchus via bronchoscopy. After the patient was diagnosed with ...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 信件


    authors: Kuzucuoglu M,Karamustafaoglu YA,Cicin I,Yoruk Y

    更新日期:2014-04-01 00:00:00

  • 125I brachytherapy seeds implantation for an inoperable large retroperitoneal leiomyosarcoma.

    abstract::Retroperitoneal leiomyosarcoma is relatively uncommon. Leiomyosarcoma has accounted for about 5%-10% of soft-tissue sarcoma, and 1/2-2/3 of the primary lesions were retroperitoneal, with a cumulative 5-year survival rate of only 35%.Leiomyosarcoma is one kind of soft-tissue sarcoma with the lowest survival rates due t...

    journal_title:Journal of cancer research and therapeutics



    authors: Di X,Liang Y,Yu H,Zhao J,Wang Z,Zhao J,Sui A,Zhang H,Wang J

    更新日期:2020-01-01 00:00:00

  • Clinical study on thalidomide combined with cinobufagin to treat lung cancer cachexia.

    abstract:Objective:To discuss and assess the clinical value of treating lung cancer cachexia with thalidomide combined with cinobufagin.s. Methods:A cohort of 54 patients, who were diagnosed with lung carcinoma, was randomly divided into two groups, a trial group and a control group, respectively. The trial group was given 150...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Xie M,Chen X,Qin S,Bao Y,Bu K,Lu Y

    更新日期:2018-01-01 00:00:00

  • Characterization and localization of c-kit and epidermal growth factor receptor in different patterns of adenoid cystic carcinoma.

    abstract:INTRODUCTION:Adenoid cystic carcinoma (ACC) is malignant neoplasm of the salivary glands. It accounts for most cases of minor salivary gland malignancies and a substantial proportion of parotid and submandibular gland malignancies. ACC is associated with a high mortality rate, and it often recurs after prolonged period...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Jain A,Shetty DC,Rathore AS,Kumar K

    更新日期:2016-04-01 00:00:00

  • Splenic infarct as a diagnostic pitfall in radiology.

    abstract::Follow-up of colorectal carcinoma after therapy is based on symptoms, tumor markers, and imaging studies. Clinicians sometimes face diagnostic dilemmas because of unusual presentations on the imaging modalities coupled with rising serum markers. We report a case of colorectal carcinoma that presented with gastrointest...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 杂志文章


    authors: Joshi SC,Pant I,Shukla AN,Anshari MA

    更新日期:2008-04-01 00:00:00

  • Primary malignant lymphoma of the parotid gland.

    abstract::Lymphoma of the salivary gland accounts for 5% of cases of extranodal lymphoma and 10% of malignant salivary gland tumors. Most primary salivary gland lymphomas are B marginal zone lymphomas arising on a background of sialadenitis associated with an autoimmune disorder such as Sjorgen's syndrome. This report describes...

    journal_title:Journal of cancer research and therapeutics

    pub_type: 信件


    authors: Metikurke SH,Krishnappa R,Ramachar SM,Arora I

    更新日期:2012-10-01 00:00:00