Abstract：
:Processive chitinase is a linear molecular motor which moves on the surface of crystalline chitin driven by processive hydrolysis of single chitin chain. Here, we analyse the mechanism underlying unidirectional movement of Serratia marcescens chitinase A (SmChiA) using high-precision single-molecule imaging, X-ray crystallography, and all-atom molecular dynamics simulation. SmChiA shows fast unidirectional movement of ~50 nm s-1 with 1 nm forward and backward steps, consistent with the length of reaction product chitobiose. Analysis of the kinetic isotope effect reveals fast substrate-assisted catalysis with time constant of ~3 ms. Decrystallization of the single chitin chain from crystal surface is the rate-limiting step of movement with time constant of ~17 ms, achieved by binding free energy at the product-binding site of SmChiA. Our results demonstrate that SmChiA operates as a burnt-bridge Brownian ratchet wherein the Brownian motion along the single chitin chain is rectified forward by substrate-assisted catalysis.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Nakamura A,Okazaki KI,Furuta T,Sakurai M,Iino Rdoi
10.1038/s41467-018-06362-3subject
Has Abstractpub_date
2018-09-19 00:00:00pages
3814issue
1issn
2041-1723pii
10.1038/s41467-018-06362-3journal_volume
9pub_type
杂志文章abstract：:Autoantibodies have been associated with autoimmune diseases. However, studies have identified autoantibodies in healthy donors (HD) who do not develop autoimmune disorders. Here we provide evidence of a network of immunoglobulin G (IgG) autoantibodies targeting G protein-coupled receptors (GPCR) in HD compared to pat...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-07598-9
更新日期：2018-12-06 00:00:00
abstract：:Centromeres are specified epigenetically through the deposition of the centromere-specific histone H3 variant CENP-A. However, how additional epigenetic features are involved in centromere specification is unknown. Here, we find that histone H4 Lys5 and Lys12 acetylation (H4K5ac and H4K12ac) primarily occur within the...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms13465
更新日期：2016-11-04 00:00:00
abstract：:Cladodontomorph sharks are Palaeozoic stem chondrichthyans thought to go extinct at the end-Permian mass extinction. This extinction preceded the diversification of euselachians, including modern sharks. Here we describe an outer-platform cladodontomorph shark tooth assemblage from the Early Cretaceous of southern Fra...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms3669
更新日期：2013-01-01 00:00:00
abstract：:The principles governing the recruitment of interneurons during acceleration in vertebrate locomotion are unknown. In the mouse, the V2a spinal interneurons are dispensable for left-right coordination at low locomotor frequencies, but their function is essential for maintaining left-right coordination at high frequenc...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms1276
更新日期：2011-01-01 00:00:00
abstract：:Among the perovskite oxide family, KTaO3 (KTO) has recently attracted considerable interest as a possible system for the realization of the Rashba effect. In this work, we report a novel conducting interface by placing KTO with another insulator, LaVO3 (LVO) and report planar Hall effect (PHE) and anisotropic magnetor...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-14689-z
更新日期：2020-02-13 00:00:00
abstract：:The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumet...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms14843
更新日期：2017-03-22 00:00:00
abstract：:Bryostatin is in clinical trials for Alzheimer's disease, cancer, and HIV/AIDS eradication. It binds to protein kinase C competitively with diacylglycerol, the endogenous protein kinase C regulator, and plant-derived phorbol esters, but each ligand induces different activities. Determination of the structural origin f...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-016-0015-8
更新日期：2017-02-23 00:00:00
abstract：:Alteration of the gut microbiota has been associated with different gastrointestinal disorders. Normobiosis restoration by faecal microbiota transplantation (FMT) is considered a promising therapeutic approach, even if the mechanisms underlying its efficacy are at present largely unknown. Here we sought to elucidate t...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-07359-8
更新日期：2018-12-05 00:00:00
abstract：:All-inorganic perovskites have a special place in halide perovskite family because of their potential for better stability. However, the representative cesium lead iodide (CsPbI3) is metastable and spontaneously converts to the non-perovskite structure at room temperature. Here, we demonstrate that what appears to be ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-07204-y
更新日期：2018-11-14 00:00:00
abstract：:Quantum magnets provide the simplest example of strongly interacting quantum matter, yet they continue to resist a comprehensive understanding above one spatial dimension. We explore a promising framework in two dimensions, the Dirac spin liquid (DSL) - quantum electrodynamics (QED3) with 4 Dirac fermions coupled to p...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-11727-3
更新日期：2019-09-18 00:00:00
abstract：:[This corrects the article DOI: 10.1038/ncomms5802.]. ...
journal_title：Nature communications
pub_type： 已发布勘误
doi：10.1038/ncomms16177
更新日期：2017-11-29 00:00:00
abstract：:A longstanding goal of synthetic biology has been the programmable control of cellular functions. Central to this is the creation of versatile regulatory toolsets that allow for programmable control of gene expression. Of the many regulatory molecules available, RNA regulators offer the intriguing possibility of de no...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-01082-6
更新日期：2017-10-19 00:00:00
abstract：:Textiles that are capable of harvesting biomechanical energy via triboelectric effects are of interest for self-powered wearable electronics. Fabrication of conformable and durable textiles with high triboelectric outputs remains challenging. Here we propose a washable skin-touch-actuated textile-based triboelectric n...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-06759-0
更新日期：2018-10-15 00:00:00
abstract：:During meiosis, homologous chromosomes synapse and recombine at sites marked by the binding of the mismatch repair protein MLH1. In hexaploid wheat, the Ph1 locus has a major effect on whether crossover occurs between homologues or between related homoeologues. Here we report that--in wheat-rye hybrids where homologue...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms5580
更新日期：2014-08-06 00:00:00
abstract：:Ions are ubiquitous biological regulators playing a key role for vital processes in animals and plants. The combined detection of ion concentration and real-time monitoring of small variations with respect to the resting conditions is a multiscale functionality providing important information on health states. This mu...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-17547-0
更新日期：2020-07-27 00:00:00
abstract：:Acetic acid is an important bulk chemical that is currently produced via methanol carbonylation using fossil based CO. Synthesis of acetic acid from the renewable and cheap CO2 is of great importance, but state of the art routes encounter difficulties, especially in reaction selectivity and activity. Here we report a ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms11481
更新日期：2016-05-11 00:00:00
abstract：:Cleavage of transfer (t)RNA and ribosomal (r)RNA are critical and conserved steps of translational control for cells to overcome varied environmental stresses. However, enzymes that are responsible for this event have not been fully identified in high eukaryotes. Here, we report a mammalian tRNA/rRNA-targeting endorib...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-03544-x
更新日期：2018-03-21 00:00:00
abstract：:Heterozygous germline mutations in BRCA2 predispose to breast and ovarian cancer. Contrary to non-cancerous cells, where BRCA2 deletion causes cell cycle arrest or cell death, tumors carrying BRCA2 inactivation continue to proliferate. Here we set out to investigate adaptation to loss of BRCA2 focusing on genome-wide ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-11048-5
更新日期：2019-07-17 00:00:00
abstract：:Topological defects in matter behave collectively to form highly non-trivial structures called topological textures that are characterised by conserved quantities such as the winding number. Here we show that an epitaxial ferroelectric square nanoplate of bismuth ferrite subjected to a large strain gradient (as much a...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-02813-5
更新日期：2018-01-26 00:00:00
abstract：:Blood vessel expansion is driven by sprouting angiogenesis of endothelial cells, and is essential for development, wound healing and disease. Membrane-localized vascular endothelial growth factor receptor-1 (mVEGFR1) is an endothelial cell-intrinsic decoy receptor that negatively modulates blood vessel morphogenesis. ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms15699
更新日期：2017-06-07 00:00:00
abstract：:To fully exploit the microbial genome resources, a high-throughput experimental platform is needed to associate genes with phenotypes at the genome level. We present here a novel method that enables investigation of the cellular consequences of repressing individual transcripts based on the CRISPR interference (CRISPR...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-04899-x
更新日期：2018-06-26 00:00:00
abstract：:Distal appendages (DAPs) are nanoscale, pinwheel-like structures protruding from the distal end of the centriole that mediate membrane docking during ciliogenesis, marking the cilia base around the ciliary gate. Here we determine a super-resolved multiplex of 16 centriole-distal-end components. Surprisingly, rather th...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-04469-1
更新日期：2018-05-22 00:00:00
abstract：:Combinatorial polyvalent contacts of histone-binding domains or readers commonly mediate localization and activities of chromatin-associated proteins. A pair of readers, the PHD fingers of the protein CHD4, has been shown to bivalently recognize histone H3 tails. Here we describe a mechanism by which these linked but ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-01598-x
更新日期：2017-11-14 00:00:00
abstract：:The mammalian neocortex is subdivided into cytoarchitectural areas with distinct connectivity, gene expression and neural functions. Areal identity is initially specified by rostrocaudal and mediolateral gene expression gradients in neuroepithelial and radial glial progenitors (the 'protomap'). On further differentiat...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms6632
更新日期：2014-12-05 00:00:00
abstract：:A nest of begging chicks invites an intuitive explanation: needy chicks want to be fed and parents want to feed them. Surprisingly, however, in a quarter of species studied, parents ignore begging chicks. Furthermore, parents in some species even neglect smaller chicks that beg more, and preferentially feed the bigges...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms10985
更新日期：2016-03-29 00:00:00
abstract：:Electronic structure heterogeneities are ubiquitous in two-dimensional graphene and profoundly impact the transport properties of this material. Here we show the mapping of discrete electronic domains within a single graphene sheet using scanning transmission X-ray microscopy in conjunction with ab initio density func...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms1376
更新日期：2011-06-28 00:00:00
abstract：:Replication-Factor-C (RFC) and RFC-like complexes (RLCs) mediate chromatin engagement of the proliferating cell nuclear antigen (PCNA). It remains controversial how RFC and RLCs cooperate to regulate PCNA loading and unloading. Here, we show the distinct PCNA loading or unloading activity of each clamp loader. ATAD5-R...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-10376-w
更新日期：2019-06-03 00:00:00
abstract：:Columnar joints form by cracking during cooling-induced contraction of lava, allowing hydrothermal fluid circulation. A lack of direct observations of their formation has led to ambiguity about the temperature window of jointing and its impact on fluid flow. Here we develop a novel thermo-mechanical experiment to disc...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-03842-4
更新日期：2018-04-12 00:00:00
abstract：:Plant phytochromes are thought to transduce light signals by mediating the degradation of phytochrome-interacting transcription factors (PIFs) through the N-terminal photosensory module, while the C-terminal module, including a histidine kinase-related domain (HKRD), does not participate in signaling. Here we show tha...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-02062-6
更新日期：2017-12-04 00:00:00
abstract：:LNK (SH2B3) is a key negative regulator of JAK-STAT signaling which has been extensively studied in malignant hematopoietic diseases. We found that LNK is significantly elevated in cutaneous melanoma; this elevation is correlated with hyperactive signaling of the RAS-RAF-MEK pathway. Elevated LNK enhances cell growth ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-09711-y
更新日期：2019-05-20 00:00:00
