Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis.

Abstract:

:Inflammatory bowel disease (IBD) is prevalent, but the mechanisms underlying disease development remain elusive. We identify a role for the E3 ubiquitin ligase RNF5 in IBD. Intestinal epithelial cells (IECs) express a high level of RNF5, while the colon of Rnf5-/- mice exhibits activated dendritic cells and intrinsic inflammation. Rnf5-/- mice exhibit severe acute colitis following dextran sodium sulfate (DSS) treatment. S100A8 is identified as an RNF5 substrate, resulting in S100A8 ubiquitination and proteasomal-dependent degradation that is attenuated upon inflammatory stimuli. Loss of RNF5 from IECs leads to enhanced S100A8 secretion, which induces mucosal CD4+ T cells, resulting in Th1 pro-inflammatory responses. Administration of S100A8-neutralizing antibodies to DSS-treated Rnf5-/- mice attenuates acute colitis development and increases survival. An inverse correlation between RNF5 and S100A8 protein expression in IECs of IBD patients coincides with disease severity. Collectively, RNF5-mediated regulation of S100A8 stability in IECs is required for the maintenance of intestinal homeostasis.

journal_name

Cell Rep

journal_title

Cell reports

authors

Fujita Y,Khateb A,Li Y,Tinoco R,Zhang T,Bar-Yoseph H,Tam MA,Chowers Y,Sabo E,Gerassy-Vainberg S,Starosvetsky E,James B,Brown K,Shen-Orr SS,Bradley LM,Tessier PA,Ronai ZA

doi

10.1016/j.celrep.2018.08.057

subject

Has Abstract

pub_date

2018-09-18 00:00:00

pages

3296-3311.e6

issue

12

issn

2211-1247

pii

S2211-1247(18)31344-5

journal_volume

24

pub_type

杂志文章
  • A Batf3/Nlrp3/IL-18 Axis Promotes Natural Killer Cell IL-10 Production during Listeria monocytogenes Infection.

    abstract::The bacterial pathogen Listeria monocytogenes (Lm) capitalizes on natural killer (NK) cell production of regulatory interleukin (IL)-10 to establish severe systemic infections. Here, we identify regulators of this IL-10 secretion. We show that IL-18 signals to NK cells license their ability to produce IL-10. IL-18 act...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2018.04.106

    authors: Clark SE,Schmidt RL,McDermott DS,Lenz LL

    更新日期:2018-05-29 00:00:00

  • An Antifungal Combination Matrix Identifies a Rich Pool of Adjuvant Molecules that Enhance Drug Activity against Diverse Fungal Pathogens.

    abstract::There is an urgent need to identify new treatments for fungal infections. By combining sub-lethal concentrations of the known antifungals fluconazole, caspofungin, amphotericin B, terbinafine, benomyl, and cyprodinil with ∼3,600 compounds in diverse fungal species, we generated a deep reservoir of chemical-chemical in...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.10.018

    authors: Robbins N,Spitzer M,Yu T,Cerone RP,Averette AK,Bahn YS,Heitman J,Sheppard DC,Tyers M,Wright GD

    更新日期:2015-11-17 00:00:00

  • Tet3 Reads 5-Carboxylcytosine through Its CXXC Domain and Is a Potential Guardian against Neurodegeneration.

    abstract::We report that the mammalian 5-methylcytosine (5mC) oxidase Tet3 exists as three major isoforms and characterized the full-length isoform containing an N-terminal CXXC domain (Tet3FL). This CXXC domain binds to unmethylated CpGs, but, unexpectedly, its highest affinity is toward 5-carboxylcytosine (5caC). We determine...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.12.044

    authors: Jin SG,Zhang ZM,Dunwell TL,Harter MR,Wu X,Johnson J,Li Z,Liu J,Szabó PE,Lu Q,Xu GL,Song J,Pfeifer GP

    更新日期:2016-01-26 00:00:00

  • A Late Phase of Long-Term Synaptic Depression in Cerebellar Purkinje Cells Requires Activation of MEF2.

    abstract::The MEF2 family of transcription factors restricts excitatory synapse number in an activity-dependent fashion during development, yet MEF2 has not been implicated in long-term synaptic depression (LTD), which is thought to initiate synapse elimination. Mutations in MEF2 pathways are implicated in autism spectrum disor...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2019.01.004

    authors: Andzelm MM,Vanness D,Greenberg ME,Linden DJ

    更新日期:2019-01-29 00:00:00

  • SIRT7 represses Myc activity to suppress ER stress and prevent fatty liver disease.

    abstract::Nonalcoholic fatty liver disease is the most common chronic liver disorder in developed countries. Its pathogenesis is poorly understood, and therapeutic options are limited. Here, we show that SIRT7, an NAD(+)-dependent H3K18Ac deacetylase, functions at chromatin to suppress ER stress and prevent the development of f...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2013.10.007

    authors: Shin J,He M,Liu Y,Paredes S,Villanova L,Brown K,Qiu X,Nabavi N,Mohrin M,Wojnoonski K,Li P,Cheng HL,Murphy AJ,Valenzuela DM,Luo H,Kapahi P,Krauss R,Mostoslavsky R,Yancopoulos GD,Alt FW,Chua KF,Chen D

    更新日期:2013-11-14 00:00:00

  • Analysis of in vitro insulin-resistance models and their physiological relevance to in vivo diet-induced adipose insulin resistance.

    abstract::Diet-induced obesity (DIO) predisposes individuals to insulin resistance, and adipose tissue has a major role in the disease. Insulin resistance can be induced in cultured adipocytes by a variety of treatments, but what aspects of the in vivo responses are captured by these models remains unknown. We use global RNA se...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2013.08.039

    authors: Lo KA,Labadorf A,Kennedy NJ,Han MS,Yap YS,Matthews B,Xin X,Sun L,Davis RJ,Lodish HF,Fraenkel E

    更新日期:2013-10-17 00:00:00

  • L-selectin Is Essential for Delivery of Activated CD8(+) T Cells to Virus-Infected Organs for Protective Immunity.

    abstract::Cytotoxic CD8(+) T lymphocytes play a critical role in the host response to infection by viruses. The ability to secrete cytotoxic chemicals and cytokines is considered pivotal for eliminating virus. Of equal importance is how effector CD8(+) T cells home to virus-infected tissues. L-selectin has not been considered i...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.12.090

    authors: Mohammed RN,Watson HA,Vigar M,Ohme J,Thomson A,Humphreys IR,Ager A

    更新日期:2016-02-02 00:00:00

  • A RhoA-FRET Biosensor Mouse for Intravital Imaging in Normal Tissue Homeostasis and Disease Contexts.

    abstract::The small GTPase RhoA is involved in a variety of fundamental processes in normal tissue. Spatiotemporal control of RhoA is thought to govern mechanosensing, growth, and motility of cells, while its deregulation is associated with disease development. Here, we describe the generation of a RhoA-fluorescence resonance e...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.09.022

    authors: Nobis M,Herrmann D,Warren SC,Kadir S,Leung W,Killen M,Magenau A,Stevenson D,Lucas MC,Reischmann N,Vennin C,Conway JRW,Boulghourjian A,Zaratzian A,Law AM,Gallego-Ortega D,Ormandy CJ,Walters SN,Grey ST,Bailey J,Chta

    更新日期:2017-10-03 00:00:00

  • Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

    abstract::Glutamine is thought to play an important role in cancer cells by being deaminated via glutaminolysis to α-ketoglutarate (aKG) to fuel the tricarboxylic acid (TCA) cycle. Supporting this notion, aKG supplementation can restore growth/survival of glutamine-deprived cells. However, pancreatic cancers are often poorly va...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2019.09.056

    authors: Bott AJ,Shen J,Tonelli C,Zhan L,Sivaram N,Jiang YP,Yu X,Bhatt V,Chiles E,Zhong H,Maimouni S,Dai W,Velasquez S,Pan JA,Muthalagu N,Morton J,Anthony TG,Feng H,Lamers WH,Murphy DJ,Guo JY,Jin J,Crawford HC,Zhang

    更新日期:2019-10-29 00:00:00

  • PARTICLE, a Triplex-Forming Long ncRNA, Regulates Locus-Specific Methylation in Response to Low-Dose Irradiation.

    abstract::Exposure to low-dose irradiation causes transiently elevated expression of the long ncRNA PARTICLE (gene PARTICLE, promoter of MAT2A-antisense radiation-induced circulating lncRNA). PARTICLE affords both a cytosolic scaffold for the tumor suppressor methionine adenosyltransferase (MAT2A) and a nuclear genetic platform...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.03.043

    authors: O'Leary VB,Ovsepian SV,Carrascosa LG,Buske FA,Radulovic V,Niyazi M,Moertl S,Trau M,Atkinson MJ,Anastasov N

    更新日期:2015-04-21 00:00:00

  • Peripheral Elevation of a Klotho Fragment Enhances Brain Function and Resilience in Young, Aging, and α-Synuclein Transgenic Mice.

    abstract::Cognitive dysfunction and decreased mobility from aging and neurodegenerative conditions, such as Parkinson and Alzheimer diseases, are major biomedical challenges in need of more effective therapies. Increasing brain resilience may represent a new treatment strategy. Klotho, a longevity factor, enhances cognition whe...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.07.024

    authors: Leon J,Moreno AJ,Garay BI,Chalkley RJ,Burlingame AL,Wang D,Dubal DB

    更新日期:2017-08-08 00:00:00

  • In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9.

    abstract::CRISPR/Cas-based technologies have revolutionized genetic approaches to addressing a wide range of neurobiological questions. The ability of CRISPR/Cas to introduce mutations into target genes allows us to perform in vivo loss-of-function experiments without generating genetically engineered mice. However, the lack of...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2019.06.038

    authors: Steinecke A,Kurabayashi N,Hayano Y,Ishino Y,Taniguchi H

    更新日期:2019-07-09 00:00:00

  • CDK8 Regulates Insulin Secretion and Mediates Postnatal and Stress-Induced Expression of Neuropeptides in Pancreatic β Cells.

    abstract::Cyclin-dependent kinases (CDKs) contribute to vital cellular processes including cell cycle regulation. Loss of CDKs is associated with impaired insulin secretion and β cell survival; however, the function of CDK8 in β cells remains elusive. Here, we report that genetic ablation of Cdk8 improves glucose tolerance by i...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2019.08.025

    authors: Xue J,Scotti E,Stoffel M

    更新日期:2019-09-10 00:00:00

  • WRNIP1 Is Recruited to DNA Interstrand Crosslinks and Promotes Repair.

    abstract::The Fanconi anemia (FA) pathway repairs DNA interstrand crosslinks (ICLs). Many FA proteins are recruited to ICLs in a timely fashion so that coordinated repair can occur. However, the mechanism of this process is poorly understood. Here, we report the purification of a FANCD2-containing protein complex with multiple ...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2020.107850

    authors: Socha A,Yang D,Bulsiewicz A,Yaprianto K,Kupculak M,Liang CC,Hadjicharalambous A,Wu R,Gygi SP,Cohn MA

    更新日期:2020-07-07 00:00:00

  • Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease.

    abstract::Graft-versus-host disease (GVHD) limits the success of allogeneic hematopoietic cell transplantation (allo-HCT). Lysosomal acid lipase (LAL) mediates the intrinsic lipolysis of cells to generate free fatty acids (FFAs), which play an essential role in the development, proliferation, and function of T cells. Here, we f...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2020.108316

    authors: Nguyen HD,Ticer T,Bastian D,Kuril S,Li H,Du H,Yan C,Yu XZ

    更新日期:2020-10-27 00:00:00

  • HJURP involvement in de novo CenH3(CENP-A) and CENP-C recruitment.

    abstract::Although our understanding of centromere maintenance, marked by the histone H3 variant CenH3(CENP-A) in most eukaryotes, has progressed, the mechanism underlying the de novo formation of centromeres remains unclear. We used a synthetic system to dissect how CenH3(CENP-A) contributes to the accumulation of CENP-C and C...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.03.013

    authors: Tachiwana H,Müller S,Blümer J,Klare K,Musacchio A,Almouzni G

    更新日期:2015-04-07 00:00:00

  • T-Bet+ IgM Memory Cells Generate Multi-lineage Effector B Cells.

    abstract::Immunoglobulin M (IgM) memory cells undergo differentiation in germinal centers following antigen challenge, but the full effector cell potential of these cells is unknown. We monitored the differentiation of enhanced yellow fluorescent protein (eYFP)-labeled CD11c+ and CD11cneg T-bet+ IgM memory cells after their tra...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2018.06.074

    authors: Kenderes KJ,Levack RC,Papillion AM,Cabrera-Martinez B,Dishaw LM,Winslow GM

    更新日期:2018-07-24 00:00:00

  • A neurodegeneration-specific gene-expression signature of acutely isolated microglia from an amyotrophic lateral sclerosis mouse model.

    abstract::Microglia are resident immune cells of the CNS that are activated by infection, neuronal injury, and inflammation. Here, we utilize flow cytometry and deep RNA sequencing of acutely isolated spinal cord microglia to define their activation in vivo. Analysis of resting microglia identified 29 genes that distinguish mic...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2013.06.018

    authors: Chiu IM,Morimoto ET,Goodarzi H,Liao JT,O'Keeffe S,Phatnani HP,Muratet M,Carroll MC,Levy S,Tavazoie S,Myers RM,Maniatis T

    更新日期:2013-07-25 00:00:00

  • Intestinal IL-17R Signaling Constrains IL-18-Driven Liver Inflammation by the Regulation of Microbiome-Derived Products.

    abstract::Interleukin (IL)-17 signaling to the intestinal epithelium regulates the intestinal microbiome. Given the reported links between intestinal dysbiosis, bacterial translocation, and liver disease, we hypothesize that intestinal IL-17R signaling plays a critical role in mitigating hepatic inflammation. To test this, we s...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2019.10.042

    authors: Castillo-Dela Cruz P,Wanek AG,Kumar P,An X,Elsegeiny W,Horne W,Fitch A,Burr AHP,Gopalakrishna KP,Chen K,Methé BA,Canna SW,Hand TW,Kolls JK

    更新日期:2019-11-19 00:00:00

  • Experience-Dependent Equilibration of AMPAR-Mediated Synaptic Transmission during the Critical Period.

    abstract::Experience-dependent synapse refinement is essential for functional optimization of neural circuits. However, how sensory experience sculpts excitatory synaptic transmission is poorly understood. Here, we show that despite substantial remodeling of synaptic connectivity, AMPAR-mediated synaptic transmission remains at...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2016.12.084

    authors: Han KS,Cooke SF,Xu W

    更新日期:2017-01-24 00:00:00

  • Clonal Heterogeneity Influences the Fate of New Adaptive Mutations.

    abstract::The joint contribution of pre-existing and de novo genetic variation to clonal adaptation is poorly understood but essential to designing successful antimicrobial or cancer therapies. To address this, we evolve genetically diverse populations of budding yeast, S. cerevisiae, consisting of diploid cells with unique hap...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.09.046

    authors: Vázquez-García I,Salinas F,Li J,Fischer A,Barré B,Hallin J,Bergström A,Alonso-Perez E,Warringer J,Mustonen V,Liti G

    更新日期:2017-10-17 00:00:00

  • Antagonism between retinoic acid and fibroblast growth factor signaling during limb development.

    abstract::The vitamin A metabolite retinoic acid (RA) provides patterning information during vertebrate embryogenesis, but the mechanism through which RA influences limb development is unclear. During patterning of the limb proximodistal axis (upper limb to digits), avian studies suggest that a proximal RA signal generated in t...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2013.03.036

    authors: Cunningham TJ,Zhao X,Sandell LL,Evans SM,Trainor PA,Duester G

    更新日期:2013-05-30 00:00:00

  • Cooperative Transcriptional Activation of Antimicrobial Genes by STAT and NF-κB Pathways by Concerted Recruitment of the Mediator Complex.

    abstract::The transcriptional response to infection with the bacterium Listeria monocytogenes (Lm) requires cooperative signals of the type I interferon (IFN-I)-stimulated JAK-STAT and proinflammatory NF-κB pathways. Using ChIP-seq analysis, we define genes induced in Lm-infected macrophages through synergistic transcriptional ...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2015.06.021

    authors: Wienerroither S,Shukla P,Farlik M,Majoros A,Stych B,Vogl C,Cheon H,Stark GR,Strobl B,Müller M,Decker T

    更新日期:2015-07-14 00:00:00

  • A polymorphic enhancer near GREM1 influences bowel cancer risk through differential CDX2 and TCF7L2 binding.

    abstract::A rare germline duplication upstream of the bone morphogenetic protein antagonist GREM1 causes a Mendelian-dominant predisposition to colorectal cancer (CRC). The underlying disease mechanism is strong, ectopic GREM1 overexpression in the intestinal epithelium. Here, we confirm that a common GREM1 polymorphism, rs1696...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2014.07.020

    authors: Lewis A,Freeman-Mills L,de la Calle-Mustienes E,Giráldez-Pérez RM,Davis H,Jaeger E,Becker M,Hubner NC,Nguyen LN,Zeron-Medina J,Bond G,Stunnenberg HG,Carvajal JJ,Gomez-Skarmeta JL,Leedham S,Tomlinson I

    更新日期:2014-08-21 00:00:00

  • Transcriptomic Hallmarks of Tumor Plasticity and Stromal Interactions in Brain Metastasis.

    abstract::The brain is a major site of relapse for several cancers, yet deciphering the mechanisms of brain metastasis remains a challenge because of the complexity of the brain tumor microenvironment (TME). To define the molecular landscape of brain metastasis from intact tissue in vivo, we employ an RNA-sequencing-based appro...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2019.03.085

    authors: Wingrove E,Liu ZZ,Patel KD,Arnal-Estapé A,Cai WL,Melnick MA,Politi K,Monteiro C,Zhu L,Valiente M,Kluger HM,Chiang VL,Nguyen DX

    更新日期:2019-04-23 00:00:00

  • Toll-like Receptor 2 Facilitates Oxidative Damage-Induced Retinal Degeneration.

    abstract::Retinal degeneration is a form of neurodegenerative disease and is the leading cause of vision loss globally. The Toll-like receptors (TLRs) are primary components of the innate immune system involved in signal transduction. Here we show that TLR2 induces complement factors C3 and CFB, the common and rate-limiting fac...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2020.01.064

    authors: Mulfaul K,Ozaki E,Fernando N,Brennan K,Chirco KR,Connolly E,Greene C,Maminishkis A,Salomon RG,Linetsky M,Natoli R,Mullins RF,Campbell M,Doyle SL

    更新日期:2020-02-18 00:00:00

  • Targeting Ras-Driven Cancer Cell Survival and Invasion through Selective Inhibition of DOCK1.

    abstract::Oncogenic Ras plays a key role in cancer initiation but also contributes to malignant phenotypes by stimulating nutrient uptake and promoting invasive migration. Because these latter cellular responses require Rac-mediated remodeling of the actin cytoskeleton, we hypothesized that molecules involved in Rac activation ...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.04.016

    authors: Tajiri H,Uruno T,Shirai T,Takaya D,Matsunaga S,Setoyama D,Watanabe M,Kukimoto-Niino M,Oisaki K,Ushijima M,Sanematsu F,Honma T,Terada T,Oki E,Shirasawa S,Maehara Y,Kang D,Côté JF,Yokoyama S,Kanai M,Fukui Y

    更新日期:2017-05-02 00:00:00

  • Oxytocin Modulates Nociception as an Agonist of Pain-Sensing TRPV1.

    abstract::Oxytocin is a hormone with various actions. Oxytocin-containing parvocellular neurons project to the brainstem and spinal cord. Oxytocin release from these neurons suppresses nociception of inflammatory pain, the molecular mechanism of which remains unclear. Here, we report that the noxious stimulus receptor TRPV1 is ...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2017.10.063

    authors: Nersesyan Y,Demirkhanyan L,Cabezas-Bratesco D,Oakes V,Kusuda R,Dawson T,Sun X,Cao C,Cohen AM,Chelluboina B,Veeravalli KK,Zimmermann K,Domene C,Brauchi S,Zakharian E

    更新日期:2017-11-07 00:00:00

  • Bat-derived influenza hemagglutinin H17 does not bind canonical avian or human receptors and most likely uses a unique entry mechanism.

    abstract::A new influenza-like virus genome (H17N10) was recently discovered in bats and offers a new perspective about the origin and evolution of influenza viruses. The viral envelope glycoprotein hemagglutinin (HA) is responsible for influenza virus receptor binding, fusion, and entry into the cell; therefore, the structure ...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2013.01.025

    authors: Sun X,Shi Y,Lu X,He J,Gao F,Yan J,Qi J,Gao GF

    更新日期:2013-03-28 00:00:00

  • Vitamin D Receptor Controls Cell Stemness in Acute Myeloid Leukemia and in Normal Bone Marrow.

    abstract::Vitamin D (VD) is a known differentiating agent, but the role of VD receptor (VDR) is still incompletely described in acute myeloid leukemia (AML), whose treatment is based mostly on antimitotic chemotherapy. Here, we present an unexpected role of VDR in normal hematopoiesis and in leukemogenesis. Limited VDR expressi...

    journal_title:Cell reports

    pub_type: 杂志文章

    doi:10.1016/j.celrep.2019.12.055

    authors: Paubelle E,Zylbersztejn F,Maciel TT,Carvalho C,Mupo A,Cheok M,Lieben L,Sujobert P,Decroocq J,Yokoyama A,Asnafi V,Macintyre E,Tamburini J,Bardet V,Castaigne S,Preudhomme C,Dombret H,Carmeliet G,Bouscary D,Ginzburg YZ

    更新日期:2020-01-21 00:00:00