Abstract：
:CD271, a receptor of nerve growth factor (NGF), affects the biological properties of epidermal stem cells (eSCs) which are essential for skin wound closure. Tropomyosin-receptor kinase A (TrkA), another receptor of NGF, combined with CD271 has been involved with nervous system and skin keratinocytes. However, the exact role of TrkA combined with CD271 in eSCs during skin wound closure is still unclear. This study aimed to reveal the role of TrkA in the promoting wounding-healing effect of CD271 on eSCs. We obtained CD271-vo (over-expression of CD271) eSCs by lentiviral infection. K252a was used to inhibit TrkA expression. Full-thickness skin mouse wound closure model (5 mm in diameter) was used to detect the ability of CD271 over-expressed/TrkA-deficient during wound healing. The biological characteristics of eSCs and their proliferation and apoptosis were detected using immunohistochemistry and western blot. The expressions of protein kinase B (pAkt)/Akt, phosphorylated extracellular-signal-related kinase (pERK)/ERK1/2, and c-Jun N-terminal kinase (pJNK)/JNK were also detected by western blot. We found that over-expression of CD271 promoted the biological functions of eSCs. Interestingly, over-expression of CD271 in the absence of TrkA neither promoted eSCs' migration and proliferation nor promoted wound healing in a mouse model. In addition, we observed the reduced expression of pAkt/Akt and pERK/ERK1/2 following TrkA inhibition in vitro. Our studies demonstrated that the role of TrkA in the promoting wounding-healing effect of CD271 on eSCs.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Zhang M,Zhang Y,Ding J,Li X,Zang C,Yin S,Ma J,Wang Y,Cao Ydoi
10.1007/s00403-018-1863-3subject
Has Abstractpub_date
2018-11-01 00:00:00pages
737-750issue
9eissn
0340-3696issn
1432-069Xpii
10.1007/s00403-018-1863-3journal_volume
310pub_type
杂志文章abstract：:In a previous study, impedance measurements and phoreographic response were shown to quantify significant differences between involved and non-involved skin in psoriasis. The same techniques were used to objectivate the evolution on skin condition on patients treated with dioxyanthranol, difluprednate, and photochemot...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00431132
更新日期：1979-03-31 00:00:00
abstract：:The beta-adrenergic receptors, previously shown to be present on the membranes of cultured human epidermal keratinocytes, were found to be functionally coupled to membrane-bound adenylate cyclase. Using membrane preparations, the enzyme could be activated by guanosine triphosphate (GTP), the stable GTP analog GPP(HN)p...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00407740
更新日期：1986-01-01 00:00:00
abstract：:Previous studies in mice have shown that dehydroepiandrosterone (DHEA) increases the production of Th1-associated lymphokines, and of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma), by lymphocytes. However, there are no reports concerning the effect of DHEA on the production of Th2-associated lymphokines, IL-4 ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050213
更新日期：1997-06-01 00:00:00
abstract：:Medical innovation is crucial to advancing our field, and physicians have the potential to play a leading role due to their daily patient care experiences. The objective of this study was to evaluate the interest in, and barriers to participating in innovation. Two surveys were conducted; the first cross-sectional sur...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-020-02118-6
更新日期：2020-08-09 00:00:00
abstract：:Adhesional glycoproteins of desmosomes possess asparagine-linked, complex oligosaccharide side chains. We investigated the potential of these sugars to protect the core proteins of desmosomes and corneosomes (modified stratum corneum desmosomes) against proteolysis. Isolated pig ear epidermis was exposed sequentially ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372058
更新日期：1991-01-01 00:00:00
abstract：:A 2% commercial solution formulation of 5-fluorouracil (5-FU) was significantly better at delivering 5-FU than either a 1% or 5% solution. Among single component suspension formulations the highest transdermal delivery was obtained from the vehicle in which 5-FU was least soluble, isopropyl myristate (IPM), while chan...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00402624
更新日期：1990-01-01 00:00:00
abstract：:The incidence of epithelioid sarcoma among patients with malignant soft tissue tumors is small, but the rates of recurrence and metastasis of this type of sarcoma are high. To date, effective chemotherapy for advanced epithelioid sarcoma has not been established and, furthermore, epithelioid sarcoma is known to exhibi...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030000127
更新日期：2000-06-01 00:00:00
abstract：:Three new cromones have been studied that are supposed to be better absorbed and to have a wider spectrum of anti-allergic activity than disodium cromoglycate. Pretreatment with i.d. injection of 10 microgram FPL 52758 significantly reduced the weal and flare reaction induced by specific antigen in 11 patients with at...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章
doi：10.1007/BF00403843
更新日期：1980-01-01 00:00:00
abstract：:The demonstration of cytokine mRNA expression in epidermal cells by the polymerase chain reaction technique preceded by reverse transcription (RT-PCR) requires linear test conditions (i.e. that the product obtained after amplification reflects the relative amounts of starting material) and high reproducibility, specif...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00370722
更新日期：1994-01-01 00:00:00
abstract：:Marie Unna Hereditary Hypotrichosis 1 (MUHH1; OMIM 146550), a rare monogenic condition characterized by the development of sparse, twisted hair or complete hair loss, is the consequence of mutations located in the hairless (HR) gene. We have identified a 68-year-old Hungarian woman affected by alopecia universalis and...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-012-1244-2
更新日期：2012-10-01 00:00:00
abstract：:Atopic dermatitis (AD) can be exacerbated by various factors, including emotional stress, scratching and sweating. The aim of the present study was to evaluate the hypothesis that the inflammatory reaction in AD is also neurogenic. For this purpose, the levels of vasoactive intestinal polypeptide were measured radioim...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF01106107
更新日期：1991-01-01 00:00:00
abstract：:We have examined the relationship between topical psoriasis treatment and the activity of the mixed function oxidase, aryl hydrocarbon hydroxylase (AHH), in peripheral blood monocytes and lymphocytes from 20 patients with psoriasis. These data were compared to monocyte and lymphocyte AHH activity measured in 20 normal...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00511066
更新日期：1984-01-01 00:00:00
abstract：:The pathogenetic mechanisms in vitiligo have not been completely clarified. One of the major hypotheses in the pathogenesis of vitiligo is the oxidative stress hypothesis. The active or stable phase of vitiligo is defined on the basis of the progression or appearance of new lesions in the last 3 months and the absence...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-006-0680-2
更新日期：2006-09-01 00:00:00
abstract：:Solar radiation in the UVB range is absorbed primarily by the epidermal DNA where characteristic photodamage results in altered immune responses and mutagenic lesions. UVB exposure of the skin results in a profound upregulation of the anti-inflammatory cytokine, IL-10 and suppression of contact hypersensitivity (CHS)....
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-005-0634-0
更新日期：2006-03-01 00:00:00
abstract：:Antimicrobial peptides and proteins (AMPs) play important roles in skin immune defense due to their capacity to inhibit growth of microbes. During intrauterine life, the skin immune system has to acquire the prerequisites to protect the newborn from infection in the hostile environment after birth, which includes the ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-013-1340-y
更新日期：2013-08-01 00:00:00
abstract：:Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are autoimmune blistering diseases characterized by a loss of cell-cell adhesion and by autoantibodies directed against epidermal cadherins. PF antigen has been established as desmoglein I which is located strictly on the desmosome, whereas the precise ultrastructur...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF02507101
更新日期：1996-06-01 00:00:00
abstract：:Hand warts were studied in 160 butchers. Clinical and histological studies were performed in 190 warts and virological studies in 165 warts from 104 butchers. Since we found almost perfect correlation between the histological pattern and the type of infecting virus, it was possible to evaluate the virus types in a fur...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00585924
更新日期：1987-01-01 00:00:00
abstract：:Exposure of hairless mice to the light of a germicidal lamp (254 nm) under conditions which are known to induce epidermal DNA synthesis, cell proliferation, and polyamine metabolism produced a marked increase of polyamine excretion in the urine which lasted for many days. The increase was about the same for free and a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00417146
更新日期：1981-01-01 00:00:00
abstract：:In this study, a new procedure for the cultivation of dermal vascular endothelial cells from guinea pig ear lobe was established, and specific binding of [3H]mepyramine to the cultured cell membranes was demonstrated. The vascular endothelial cells were successfully isolated from the dermis of guinea pig ear lobes by ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00493469
更新日期：1990-01-01 00:00:00
abstract：:The goal of this study is to investigate commercially available cosmetics (foundations, skin care creams) which also claim to include a sun protection factor (SPF). Are these products, which are not considered sunscreen products, helpful or could they be harmful? Using an in vitro method, we tested the effectiveness o...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-009-0974-2
更新日期：2009-09-01 00:00:00
abstract：:CCN2 plays an important role in the pathogenesis of hypertrophic scars (HTSs). Although CCN2 is involved in many fibroproliferative events, the CCN2 induction signaling pathway in HTSs is yet to be elucidated. Here, we first investigated the effect of the mitogen-activated protein kinases (MAPKs) on CCN2-induced α-smo...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-013-1334-9
更新日期：2013-07-01 00:00:00
abstract：:An ultrastructural investigation of two cutaneous lesions in a two-year-old Turkish boy with disseminated lipogranulomatosis (Farber) revealed curvilinear bodies in fibroblasts, histiocytes, and endothelial cells; "elongated membranes" in fibroblasts and endothelial cells; "zebra bodies" in endothelial cells; and spin...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00694628
更新日期：1979-10-01 00:00:00
abstract：:Plectin, a widespread cytoskeletal linker protein, is prominently expressed in basal keratinocytes of the epidermis. HD1, originally identified as a hemidesmosomal protein, has been suggested to be an isoform of or closely related to plectin, but the exact relationship between these proteins is unknown. Plectin has re...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050449
更新日期：1999-10-01 00:00:00
abstract：:The aim of this study was to screen the antioxidant, anti-hyaluronidase, and anti-elastase activity of the lyophilized juice of Cucumis sativus fruit (CSLJ). The CSLJ was subjected to DPPH and superoxide radical scavenging assay in reference to butylated hydroxytoluene. The hyaluronidase and elastase inhibitory assay ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-010-1103-y
更新日期：2011-05-01 00:00:00
abstract：:A procedure measuring the ornithine decarboxylase (ODC) activity and polyamine formation of intact neonatal mouse epidermal cells in culture has been developed and tested. Basal cells prepared from neonatal mouse epidermis were plated on round 15-mm Lux coverslips, placed in Costar 24 well culture clusters and grown a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00509038
更新日期：1982-01-01 00:00:00
abstract：:The function of microphages has been studied in two patients with chronic pyoderma vegetans by in vitro determination of phagocytosis as well as chemotaxis. The results showed a striking decrease in the chemotactic activity of the neutrophil granulocytes, a reduced phagocytosis of Candida albicans and Staphylococcus a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:The penetration and permeation of the recombinant protein plasminogen activator inhibitor type 2 (PAI-2) in two formulations, one containing a penetration enhancer, into the psoriatic and uninvolved skin of eight patients with plaque-type psoriasis were investigated. Penetration and permeation of PAI-2 were measured b...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章
doi：10.1007/s00403-003-0425-4
更新日期：2003-11-01 00:00:00
abstract：:Because of its easy accessibility, the skin is a very attractive target for gene therapy purposes. To study potential clinical applications in a preclinical setting, appropriate animal models are needed. Pig skin is very similar to human skin, and a variety of human diseases that are potentially amenable to gene thera...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-006-0653-5
更新日期：2006-06-01 00:00:00
abstract：:HLA types were determined in 19 patients and 9 healthy members of 2 Danish families with hereditary angioneurotic edema. The study revealed no connections between hereditary angioneurotic edema and the HLA system. ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00409265
更新日期：1982-01-01 00:00:00
abstract：:Vitiliginous patients undergoing photochemotherapy with TMP were less susceptible to a sun burn. To investigate this point, the MED for UV-B was measured before, 2 h, and 24 h after oral intake of TMP in 10 healthy volunteers. There was an increase of MED when UV exposure had taken place 2 h after TMP intake (P < 0.01...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00403890
更新日期：1980-01-01 00:00:00
