Abstract：
:Current saliva testing methods rely on cutting edge yet expensive techniques for the detection and analysis of genetic material, proteins and biomarkers for clinical use. However, these techniques are limited in scope and often cannot be used with complex food materials. We propose an efficient ex-vivo tool for evaluating biologically relevant interactions between food components and human saliva using sedimentation velocity analytical ultracentrifugation (SV-AUC). We evaluated macromolecular content from "unstimulated" (US) and "stimulated" (SS) samples pooled from 5 healthy volunteers. Over 90% of total saliva protein consisted of α-amylase and mucin, and up to 10% was secretory immunoglobulin A (SIgA). It was shown that α-amylase concentration increased upon parafilm stimulation, which lead to a decrease in the viscosity of saliva. Then, we used a simple food system (green tea) to evaluate changes in the salivary protein content caused by green tea polyphenols. It was found that aroma release from green tea is highly influenced by interactions between α-amylase and polyphenol epigallocatechin 3-gallate (EGCG). This interaction was found to increase the viscosity of the salivary bulk, suggested to contribute to astringency, and increased the concentrations of β-ionone, benzaldehyde and isovaleraldehyde (P < 0.01), suggested to play a significant role in the characteristic flavour of green tea.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Dinu V,Liu C,Ali J,Ayed C,Gershkovich P,Adams GG,Harding SE,Fisk IDdoi
10.1038/s41598-018-31625-wsubject
Has Abstractpub_date
2018-09-06 00:00:00pages
13350issue
1issn
2045-2322pii
10.1038/s41598-018-31625-wjournal_volume
8pub_type
杂志文章abstract：:The non-protein amino acid β-methylamino-L-alanine (BMAA) is a neurotoxin present in microalgae and shown to accumulate in the food web. BMAA has been linked to the complex neurodegenerative disorder of Guam and to increased incidents sporadic ALS. Two main neurotoxic routes are suggested; an excitotoxic by acting as ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29631
更新日期：2016-07-12 00:00:00
abstract：:TBC1D10C is a protein previously demonstrated to bind and inhibit Ras and Calcineurin. In cardiomyocytes, also CaMKII is inhibited and all three targeted enzymes are known to promote maladaptive cardiomyocyte hypertrophy. Here, in accordance with lack of Calcineurin inhibition in vivo, we did not observe a relevant an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33853
更新日期：2016-09-26 00:00:00
abstract：:Mendelian randomisation studies from Asia suggest detrimental influences of alcohol on cardiovascular risk factors, but such associations are observed mainly in men. The absence of associations of genetic variants (e.g. rs671 in ALDH2) with such risk factors in women - who drank little in these populations - provides ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18422
更新日期：2015-12-21 00:00:00
abstract：:Tractography atlas-based analysis (TABS) is a new diffusion tensor image (DTI) statistical analysis method for detecting and understanding voxel-wise white matter properties along a fiber tract. An important requisite for accurate and sensitive TABS is the availability of a deformation field that is able to register D...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12965-5
更新日期：2017-10-04 00:00:00
abstract：:Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05597
更新日期：2014-07-07 00:00:00
abstract：:Downstream analysis of genomic and transcriptomic sequence data is often executed by functional annotation that can be performed by various bioinformatics tools and biological databases. However, a full fast integrated tool is not available for such analysis. Besides, the current available software is not able to prod...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20211-9
更新日期：2018-01-29 00:00:00
abstract：:Colorectal cancer (CRC) is a common complex disease caused by the combination of genetic variants and environmental factors. Genome-wide association studies (GWAS) have been performed and reported some novel CRC susceptibility variants. However, the potential genetic mechanisms for newly identified CRC susceptibility ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25347
更新日期：2016-05-05 00:00:00
abstract：:Chronic sleep fragmentation (SF) commonly occurs in human populations, and although it does not involve circadian shifts or sleep deprivation, it markedly alters feeding behaviors ultimately promoting obesity and insulin resistance. These symptoms are known to be related to the host gut microbiota. Mice were exposed t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35405
更新日期：2016-10-14 00:00:00
abstract：:Recent severe European droughts raise the vital question: are we already experiencing measurable changes in drought likelihood that agree with climate change projections? The plethora of drought definitions compounds this question, requiring instead that we ask: how have various types of drought changed, how do these ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14283-2
更新日期：2017-10-25 00:00:00
abstract：:Viruses induce double-stranded RNA (dsRNA) in the host cells. The mammalian system has developed dsRNA-dependent recognition receptors such as RLRs that recognize the long stretches of dsRNA as PAMPs to activate interferon-mediated antiviral pathways and apoptosis in severe infection. Here we report an efficient antiv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22550
更新日期：2016-03-03 00:00:00
abstract：:The damping properties and specifically the bend losses of polymer optical fibres (POFs) have so far only been documented by experimental work, investigating bending parameters such as bending radius, length, and distance of the bends. Even though damping mechanisms and causes are well-known, no simple, generally vali...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68568-0
更新日期：2020-07-17 00:00:00
abstract：:Spin switching of organometallic complexes by ferromagnetic surfaces is an important topic in the area of molecular nanospintronics. Moreover, graphene has been shown as a 2D surface for physisorption of molecular magnets and strain engineering on graphene can tune the spin state of an iron porphyrin (FeP) molecule fr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03405
更新日期：2013-12-03 00:00:00
abstract：:Spatial tuning properties of retinal ganglion cells (RGCs) are sharpened by lateral inhibition originating at both the outer and inner plexiform layers. Lateral inhibition in the retina contributes to local contrast enhancement and sharpens edges. In this study, we used dynamic clamp recordings to examine the contribu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21966
更新日期：2016-02-24 00:00:00
abstract：:Breast cancer susceptibility gene 1 (BRCA1) located at chromosome 17q12-21 is a classic tumor suppressor gene, and has been considered as a significant role in hereditary breast cancers. Moreover, numerous studies demonstrated the methylation status of CpG islands in the promoter regions of BRCA1 gene was aberrant in ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep17869
更新日期：2015-12-08 00:00:00
abstract：:The association between active musical engagement (as leisure activity or professionally) and mental health is still unclear, with earlier studies reporting contrasting findings. Here we tested whether musical engagement predicts (1) a diagnosis of depression, anxiety, schizophrenia, bipolar or stress-related disorder...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49099-9
更新日期：2019-08-30 00:00:00
abstract：:Attention can be allocated to mental representations to select information from working memory. To date, it remains ambiguous whether such retroactive shifts of attention involve the inhibition of irrelevant information or the prioritization of relevant information. Investigating asymmetries in posterior alpha-band os...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70004-2
更新日期：2020-08-17 00:00:00
abstract：:Programmed cell death 5 (PDCD5) is believed to play a crucial role in p53 activation; however, the underlying mechanism of how PDCD5 function is regulated during apoptosis remains obscure. Here, we report that the serine/threonine phosphatase PPEF-1 interacts with and dephosphorylates PDCD5 at Ser-119, which leads to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39222
更新日期：2017-01-04 00:00:00
abstract：:Entanglement is at the heart of many unusual and counterintuitive features of quantum mechanics. Once two quantum subsystems have become entangled, it is no longer possible to ascribe an independent state to either; instead, the subsystems are completely described only as part of a greater, composite system. As a cons...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15330
更新日期：2015-10-15 00:00:00
abstract：:Future climate change has the potential to alter the distribution and prevalence of plant pathogens, which may have significant implications for both agricultural crops and natural plant communities. However, there are few long-term datasets against which modelled predictions of pathogen responses to climate change ca...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35299-2
更新日期：2018-11-21 00:00:00
abstract：:Due to the increasing environmental impact of food production, carbon footprint as an indicator can guide farmland management. This study established a method and estimated the carbon footprint of grain production in China based on life cycle analysis (LCA). The results showed that grain production has a high carbon f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04182-x
更新日期：2017-06-29 00:00:00
abstract：:Phosphoinositides play important roles in numerous intracellular membrane pathways. Little is known about the regulation or function of these lipids in rod photoreceptor cells, which have highly active membrane dynamics. Using new assays with femtomole sensitivity, we determined that whereas levels of phosphatidylinos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26978
更新日期：2016-06-01 00:00:00
abstract：:Organisms possess multiple DNA polymerases (Pols) and use each for a different purpose. One of the five Pols in Escherichia coli, DNA polymerase IV (Pol IV), encoded by the dinB gene, is known to participate in lesion bypass at certain DNA adducts. To understand how cells choose Pols when the replication fork encounte...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13080-1
更新日期：2017-10-16 00:00:00
abstract：:Enrichment of colloidal particles in continuous flow has not only numerous applications but also poses a great challenge in controlling physical forces that are required for achieving particle enrichment. Here, we for the first time experimentally demonstrate the electrokinetically-driven continuous-flow enrichment of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11473-w
更新日期：2017-09-07 00:00:00
abstract：:Improving spatial accessibility to hospitals is a major task for health care systems which can be facilitated using recent methodological improvements of spatial accessibility measures. We used the integrated floating catchment area (iFCA) method to analyze spatial accessibility of general inpatient care (internal med...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76212-0
更新日期：2020-11-05 00:00:00
abstract：:In the past few years, the meteoric development of hybrid organic-inorganic perovskite solar cells (PSC) astonished the community. The efficiency has already reached the level needed for commercialization; however, the instability hinders its deployment on the market. Here, we report a mechanism to chemically stabiliz...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30305
更新日期：2016-07-26 00:00:00
abstract：:Climate change-induced extinctions are estimated to eliminate one in six known species by the end of the century. One major factor that will contribute to these extinctions is extreme climatic events. Here, we show the ecological impacts of recent record warm air temperatures and simultaneous peak drought conditions i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60122-2
更新日期：2020-02-25 00:00:00
abstract：:This study uses retrospective forecasts made using an APEC Climate Center seasonal forecast model to investigate the cause of errors in predicting the amplitude of El Niño Southern Oscillation (ENSO)-driven sea surface temperature variability. When utilizing Bjerknes coupled stability (BJ) index analysis, enhanced err...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05221-3
更新日期：2017-07-20 00:00:00
abstract：:Living organisms employ various mechanisms to escape harm. At the cellular level, mobile cells employ movement to avoid harmful chemicals or repellents. The present study is the first to report that cells move away from the site of injury in response to local wounding. When a migrating Dictyostelium cell was locally w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39678-1
更新日期：2019-03-12 00:00:00
abstract：:Resistance of cancer cells to radiotherapy is a major clinical problem in cancer treatment. Therefore, understanding the molecular basis of cellular resistance to radiotherapy and identification of novel targets are essential for improving treatment efficacy for cancer patients. Our previous studies have demonstrated ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14723
更新日期：2015-09-30 00:00:00
abstract：:Cassava is one of the most important root crops as a reliable source of food and carbohydrates. Carbohydrate metabolism and starch accumulation in cassava storage root is a cascade process that includes large amounts of proteins and cofactors. Here, comparative proteomics were conducted in cassava root at nine develop...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19643
更新日期：2016-01-21 00:00:00
