Lysine acetylation modulates mouse sperm capacitation.

Abstract:

:Mammalian sperm are unable to fertilize the egg immediately after ejaculation. To gain fertilization competence, they need to undergo a series of modifications inside the female reproductive tract, known as capacitation. Capacitation involves several molecular events such as phosphorylation cascades, hyperpolarization of the plasma membrane and intracellular Ca2+ changes, which prepare the sperm to develop two essential features for fertilization competence: hyperactivation and acrosome reaction. Since sperm cells lack new protein biosynthesis, post-translational modification of existing proteins plays a crucial role to obtain full functionality. Here, we show the presence of acetylated proteins in murine sperm, which increase during capacitation. Pharmacological hyperacetylation of lysine residues in non-capacitated sperm induces activation of PKA, hyperpolarization of the sperm plasma membrane, CatSper opening and Ca2+ influx, all capacitation-associated molecular events. Furthermore, hyperacetylation of non-capacitated sperm promotes hyperactivation and prepares the sperm to undergo acrosome reaction. Together, these results indicate that acetylation could be involved in the acquisition of fertilization competence of mammalian sperm.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Ritagliati C,Luque GM,Stival C,Baro Graf C,Buffone MG,Krapf D

doi

10.1038/s41598-018-31557-5

subject

Has Abstract

pub_date

2018-09-06 00:00:00

pages

13334

issue

1

issn

2045-2322

pii

10.1038/s41598-018-31557-5

journal_volume

8

pub_type

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