Abstract:
:The Raman spectral features from spinal cord tissue sections of transgenic, ALS model mice and non-transgenic mice were compared using 457 nm excitation line, profiting from the favourable signal intensity obtained in the molecular fingerprint region at this wavelength. Transverse sections from four SOD1G93A mice at 75 days and from two at 90 days after birth were analysed and compared with sections of similarly aged control mice. The spectra acquired within the grey matter of tissue sections from the diseased mice is markedly different from the grey matter signature of healthy mice. In particular, we observe an intensity increase in the spectral windows 450-650 cm-1 and 1050-1200 cm-1, accompanied by an intensity decrease in the lipid contributions at ~1660 cm-1, ~1440 cm-1 and ~1300 cm-1. Axons demyelination, loss of lipid structural order and the proliferation and aggregation of branched proteoglycans are related to the observed spectral modifications. Furthermore, the grey and white matter components of the spinal cord sections could also be spectrally distinguished, based on the relative intensity of characteristic lipid and protein bands. Raman spectra acquired from the white matter regions of the SOD1G93A mice closely resembles those from control mice.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Picardi G,Spalloni A,Generosi A,Paci B,Mercuri NB,Luce M,Longone P,Cricenti Adoi
10.1038/s41598-018-31469-4subject
Has Abstractpub_date
2018-08-30 00:00:00pages
13110issue
1issn
2045-2322pii
10.1038/s41598-018-31469-4journal_volume
8pub_type
杂志文章abstract::Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a particular advantage of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46686
更新日期:2017-04-24 00:00:00
abstract::Geographical origin determination of white rice has become the major issue of food industry. However, there is still lack of a high-throughput method for rapidly and reproducibly differentiating the geographical origins of commercial white rice. In this study, we developed a method that employed lipidomics and deep le...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08892-0
更新日期:2017-08-17 00:00:00
abstract::Amyloids are highly organized fibril aggregates arise from inappropriately folded form of the protein or polypeptide precursors under both physiological as well as simulated ambience. Amyloid synthesis is a multistep process that involves formation of several metastable intermediates. Among various intermediate specie...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35645-4
更新日期:2018-11-23 00:00:00
abstract::High-throughput technologies have identified significant changes in patterns of mRNA expression over cancer development but the functional significance of these changes often rests upon the assumption that observed changes in levels of mRNA accurately reflect changes in levels of their encoded proteins. We systematica...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08502-z
更新日期:2017-08-15 00:00:00
abstract::Neural networks can efficiently encode the probability distribution of errors in an error correcting code. Moreover, these distributions can be conditioned on the syndromes of the corresponding errors. This paves a path forward for a decoder that employs a neural network to calculate the conditional distribution, then...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11266-1
更新日期:2017-09-08 00:00:00
abstract::Pregnane X receptor (PXR) regulates glucose and lipid metabolism, but little is known of the nutritional regulation of PXR function. We investigated the genome wide effects of the nutritional status on the PXR mediated gene regulation in the liver. Mice were treated with a PXR ligand pregnenolone 16α-carbonitrile (PCN...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53101-9
更新日期:2019-11-13 00:00:00
abstract::RNaseIII proteins are dsRNA-specific endonucleases involved in many important biological processes, such as small RNA processing and maturation in eukaryotes. Various small RNAs have been identified in a protozoan parasite Entamoeba histolytica. EhRNaseIII is the only RNaseIII endonuclease domain (RIIID)-containing pr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44832
更新日期:2017-03-20 00:00:00
abstract::For people with synaesthesia ordinary stimuli such as digits or letters induce concurrent experiences such as colours. Synaesthesia is associated with a memory advantage and the aim of this study was to investigate whether this advantage persists across time. We tested recognition memory of four different types of syn...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60388-6
更新日期:2020-02-26 00:00:00
abstract::The purpose of the study was to assess both anatomic and functional outcomes between short-pulse continuous wavelength and infrared micropulse lasers in the treatment of DME. This was a prospective interventional study from tertiary care eye hospital-King Khaled Eye Specialist Hospital (Riyadh, Saudi Arabia). Patients...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79699-9
更新日期:2021-01-08 00:00:00
abstract::Air pollution has been classified as a group 1 carcinogen in humans, but the underlying tumourigenic mechanisms remain unclear. In Xuanwei city of Yunnan Province, the lung cancer incidence is among the highest in China, owing to severe air pollution generated by the combustion of smoky coal, providing a unique opport...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14331
更新日期:2015-09-23 00:00:00
abstract::Systemic Lupus Erythematosus (SLE) is the prototype of autoimmune diseases, characterized by extensive gene expression perturbations in peripheral blood immune cells. Circumstantial evidence suggests that these perturbations may be due to altered epigenetic profiles and chromatin accessibility but the relationship bet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73654-4
更新日期:2020-10-06 00:00:00
abstract::Robot-assisted laparoscopic radical prostatectomy (RALRP) is a minimally invasive procedure; however, some amount of surgical trauma that can trigger systemic inflammation remains. Moreover, pneumoperitoneum during RALRP induces ischemia-reperfusion injury (IRI). Propofol, an anesthetic, is known to have anti-inflamma...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-019-41708-x
更新日期:2019-03-27 00:00:00
abstract::Silver nanowires (AgNWs) networks are promising candidates for the replacement of indium tin oxide (ITO). However, the surface roughness of the AgNWs network is still too high for its application in optoelectronic devices. In this work, we have reduced the surface roughness of the AgNWs networks to 6.4 nm, compared to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45392
更新日期:2017-03-28 00:00:00
abstract::By moving the alkoxy group on the spacer from position 5 to position 4 to add another protocol for the algorithm for intermolecular and intramolecular hydrogen bonding interactions, highly regioselectivity of noncovalent synthesis of the hydrazide-based cyclic hexamers was achieved: one out of thirteen possible isomer...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01059
更新日期:2013-01-01 00:00:00
abstract::A series of alkyl amides was detected and identified in the sedimentary record from an archaeological site at Yuchisi, Mengcheng, Anhui, China. The alkyl amides profiles change abruptly at the depth corresponding to the transition between two prehistoric cultures, which also corresponds to an abrupt change in the fatt...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15371-z
更新日期:2017-11-07 00:00:00
abstract::The importance of microbial natural products has been widely demonstrated in the search for new antibiotics. However, the functional role of microbial metabolites in nature remains to be deciphered. Several natural products are known to mediate microbial interactions through metabolic exchange. One approach to investi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05532-5
更新日期:2017-07-14 00:00:00
abstract::It has been reported that in patients with operable stage I non-small cell lung cancer (NSCLC), overall survival (OS) is better in those who undergo hypofractionated stereotactic radiation therapy (HSRT) than in those who undergo surgery. However, the reason that HSRT has a better OS has not been fully explored. Here,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04978-x
更新日期:2017-07-07 00:00:00
abstract::Loss of cardiomyocyte proliferative capacity after birth is a major obstacle for therapeutic heart regeneration in adult mammals. We and others have recently shown the importance of hypoxic in utero environments for active foetal cardiomyocyte proliferation. Here, we report the unexpected expression of novex-3, the sh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30886-9
更新日期:2018-08-17 00:00:00
abstract::This corrects the article DOI: 10.1038/srep44797. ...
journal_title:Scientific reports
pub_type: 杂志文章,已发布勘误
doi:10.1038/srep46843
更新日期:2017-06-16 00:00:00
abstract::The aim of this study was to fabricate a novel polymer-free everolimus-eluting stent with nanostructure using a femtosecond laser (FSL). The stent were coated with everolimus (EVL) using FSL and electrospinning processes. The surface was rendered hydrophobic, which negatively affected both platelet adhesion (82.1%) an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25629-9
更新日期:2018-05-09 00:00:00
abstract::In this study, the toxic effects of phenoxyethanol (Phy-Et), which is widely used in cosmetic industry, has been investigated with Allium test by means of physiological, cytogenetic, anatomical and biochemical parameters. To determine the changes in physiological reactions weight gain, relative injury rate, germinatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58170-9
更新日期:2020-01-27 00:00:00
abstract::Bio-functionalized nanoparticles with semiconducting/metallic core encapsulated in a bio- or bio-derived materials are promising for applications in biology and especially in cancer diagnostic and healing. In this report, we report a facile, single-step, first-time synthesis and in-situ functionalization strategy for ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18629
更新日期:2015-12-21 00:00:00
abstract::Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its 'melting point'. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19702-6
更新日期:2018-01-23 00:00:00
abstract::The plant selective autophagy cargo receptor neighbour of breast cancer 1 gene (NBR1) has been scarcely studied in the context of abiotic stress. We wanted to expand this knowledge by using Arabidopsis thaliana lines with constitutive ectopic overexpression of the AtNBR1 gene (OX lines) and the AtNBR1 Knock-Out (KO li...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64765-z
更新日期:2020-05-08 00:00:00
abstract::The one-stage partial nitritation and anammox process (PN/A) has been a promising microbial process to remove ammonia from wastewater especially with low carbon/nitrogen ratio. The main breakdown was the deterioration caused by overgrowth of nitrite oxidizing bacteria (NOB) resulting effluent nitrate build-up in the P...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37500
更新日期:2016-11-24 00:00:00
abstract::We have performed a genome-wide association study (GWAS) including 473 Japanese HBV (hepatitis B virus)-positive HCC (hepatocellular carcinoma) patients and 516 HBV carriers including chronic hepatitis and asymptomatic carrier individuals to identify new host genetic factors associated with HBV-derived HCC in Japanese...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26217-7
更新日期:2018-05-21 00:00:00
abstract::Microactuators are the most distinctive and challenging microdevices among micro electromechanical systems (MEMS) relative to microsensors or electronic circuits. Soft and flexible microactuators have been achieved by introducing polymers as structural materials in addition to conventional materials. Expanding the app...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50670-7
更新日期:2019-10-09 00:00:00
abstract::α-Dystroglycanopathy (α-DGP) is a group of muscular dystrophy characterized by abnormal glycosylation of α-dystroglycan (α-DG), including Fukuyama congenital muscular dystrophy (FCMD), muscle-eye-brain disease, Walker-Warburg syndrome, and congenital muscular dystrophy type 1D (MDC1D), etc. LARGE, the causative gene f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08316
更新日期:2015-02-09 00:00:00
abstract::Motivated from numerous practical applications, the present theoretical and numerical work investigates the nonlinear magnetohydrodynamic (MHD) laminar boundary layer flow of an incompressible, viscous fluid over a porous stretching sheet in the presence of suction/injection (mass transpiration). The flow characterist...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52597-5
更新日期:2019-12-06 00:00:00
abstract::Plant genomes encode several classes of small regulatory RNAs (sRNAs) that play critical roles in both development and stress responses. Panax notoginseng (Burk.) F.H. Chen (P. notoginseng) is an important traditional Chinese herbal medicinal plant species for its haemostatic effects. Therefore, the root yield of P. n...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09670-8
更新日期:2017-08-25 00:00:00