当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Metabotropic Acetylcholine and Glutamate Receptors Mediate PI(4,5)P2 Depletion and Oscillations in Hippocampal CA1 Pyramidal Neurons in situ.

Metabotropic Acetylcholine and Glutamate Receptors Mediate PI(4,5)P2 Depletion and Oscillations in Hippocampal CA1 Pyramidal Neurons in situ.

Abstract：

:The sensitivity of many ion channels to phosphatidylinositol-4,5-bisphosphate (PIP2) levels in the cell membrane suggests that PIP2 fluctuations are important and general signals modulating neuronal excitability. Yet the PIP2 dynamics of central neurons in their native environment remained largely unexplored. Here, we examined the behavior of PIP2 concentrations in response to activation of Gq-coupled neurotransmitter receptors in rat CA1 hippocampal neurons in situ in acute brain slices. Confocal microscopy of the PIP2-selective molecular sensors tubbyCT-GFP and PLCδ1-PH-GFP showed that pharmacological activation of muscarinic acetylcholine (mAChR) or group I metabotropic glutamate (mGluRI) receptors induces transient depletion of PIP2 in the soma as well as in the dendritic tree. The observed PIP2 dynamics were receptor-specific, with mAChR activation inducing stronger PIP2 depletion than mGluRI, whereas agonists of other Gαq-coupled receptors expressed in CA1 neurons did not induce measureable PIP2 depletion. Furthermore, the data show for the first time neuronal receptor-induced oscillations of membrane PIP2 concentrations. Oscillatory behavior indicated that neurons can rapidly restore PIP2 levels during persistent activation of Gq and PLC. Electrophysiological responses to receptor activation resembled PIP2 dynamics in terms of time course and receptor specificity. Our findings support a physiological function of PIP2 in regulating electrical activity.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Hackelberg S,Oliver D

doi

10.1038/s41598-018-31322-8

subject

Has Abstract

pub_date

2018-08-28 00:00:00

pages

12987

issue

1

issn

2045-2322

pii

10.1038/s41598-018-31322-8

journal_volume

8

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Carbon Quantum Dots for Zebrafish Fluorescence Imaging.

    abstract：:Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep11835

    authors： Kang YF,Li YH,Fang YW,Xu Y,Wei XM,Yin XB

    更新日期：2015-07-02 00:00:00

  • Role of supplemental foods and habitat structural complexity in persistence and coexistence of generalist predatory mites.

    abstract：:Plant traits can influence the interactions between herbivore arthropods and their natural enemies. In these interactions generalist predators are often present, preying on herbivores and also on other arthropods in the same trophic guild. Variation in the strength of intraguild predation (IGP) may be related to habit...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep14997

    authors： Pozzebon A,Loeb GM,Duso C

    更新日期：2015-10-09 00:00:00

  • Experimental study on the location of an acoustic emission source considering refraction in different media.

    abstract：:The existing acoustic emission (AE) source location methods assume that acoustic waves propagate along straight lines, and the source location is determined by average wave velocity. Because of the heterogeneity of materials, location results often fail to meet the accuracy requirement. For this reason, an AE source l...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-07371-w

    authors： Zhou ZL,Zhou J,Dong LJ,Cai X,Rui YC,Ke CT

    更新日期：2017-08-07 00:00:00

  • Lavender essential oil induces oxidative stress which modifies the bacterial membrane permeability of carbapenemase producing Klebsiella pneumoniae.

    abstract：:Misuse of antibiotics in the clinical and agricultural sectors has caused the emergence of multidrug-resistant (MDR) Klebsiella pneumoniae which contributes a threat to human health. In this study, we assessed the feasibility of lavender essential oil (LVO) as an antimicrobial agent in combinatory therapy with meropen...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-55601-0

    authors： Yang SK,Yusoff K,Thomas W,Akseer R,Alhosani MS,Abushelaibi A,Lim SH,Lai KS

    更新日期：2020-01-21 00:00:00

  • Molecular switches of the κ opioid receptor triggered by 6'-GNTI and 5'-GNTI.

    abstract：:The κ opioid receptor (κOR) is a member of G-protein-coupled receptors, and is considered as a promising drug target for treating neurological diseases. κOR selective 6'-GNTI was proved to be a G-protein biased agonist, whereas 5'-GNTI acts as an antagonist. To investigate the molecular mechanism of how these two liga...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18913

    authors： Cheng J,Sun X,Li W,Liu G,Tu Y,Tang Y

    更新日期：2016-01-08 00:00:00

  • Evolution of large area TiS2-TiO2 heterostructures and S-doped TiO2 nano-sheets on titanium foils.

    abstract：:We report a novel and facile method to synthesize sulfur-doped titanium oxide sheets and realize TiS2-TiO2 heterostructures by means of a sequential sulfurization and oxidation step in a dual-zone chemical vapor deposition furnace. The inclusion of chlorine and argon gases during the growth of such titanium-based comp...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-53651-y

    authors： Etghani SA,Ansari E,Mohajerzadeh S

    更新日期：2019-11-29 00:00:00

  • Author Correction: High velocity domain wall propagation using voltage controlled magnetic anisotropy.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-019-54255-2

    authors： Tan FN,Gan WL,Ang CCI,Wong GDH,Liu HX,Poh F,Lew WS

    更新日期：2019-11-21 00:00:00

  • Phosphorus-acquisition strategies of canola, wheat and barley in soil amended with sewage sludges.

    abstract：:Crops have different strategies to acquire poorly-available soil phosphorus (P) which are dependent on their architectural, morphological, and physiological root traits, but their capacity to enhance P acquisition varies with the type of fertilizer applied. The objective of this study was to examine how P-acquisition ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-51204-x

    authors： Nobile C,Houben D,Michel E,Firmin S,Lambers H,Kandeler E,Faucon MP

    更新日期：2019-10-16 00:00:00

  • Features of an altered AMPK metabolic pathway in Gilbert's Syndrome, and its role in metabolic health.

    abstract：:Energy metabolism, involving the ATP-dependent AMPK-PgC-Ppar pathway impacts metabolic health immensely, in that its impairment can lead to obesity, giving rise to disease. Based on observations that individuals with Gilbert's syndrome (GS; UGT1A1(*)28 promoter mutation) are generally lighter, leaner and healthier tha...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep30051

    authors： Mölzer C,Wallner M,Kern C,Tosevska A,Schwarz U,Zadnikar R,Doberer D,Marculescu R,Wagner KH

    更新日期：2016-07-21 00:00:00

  • Identifying the Stern-Gerlach force of classical electron dynamics.

    abstract：:Different classical theories are commonly applied in various branches of physics to describe the relativistic dynamics of electrons by coupled equations for the orbital motion and spin precession. Exemplarily, we benchmark the Frenkel model and the classical Foldy-Wouthuysen model with spin-dependent forces (Stern-Ger...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep31624

    authors： Wen M,Bauke H,Keitel CH

    更新日期：2016-08-22 00:00:00

  • Odour-induced analgesia mediated by hypothalamic orexin neurons in mice.

    abstract：:Various folk remedies employ certain odorous compounds with analgesic effects. In fact, linalool, a monoterpene alcohol found in lavender extracts, has been found to attenuate pain responses via subcutaneous, intraperitoneal, intrathecal, and oral administration. However, the analgesic effects of odorous compounds med...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep37129

    authors： Tashiro S,Yamaguchi R,Ishikawa S,Sakurai T,Kajiya K,Kanmura Y,Kuwaki T,Kashiwadani H

    更新日期：2016-11-15 00:00:00

  • Crystal orientation and crystal structure of paramagnetic α-Al under a pulsed electromagnetic field.

    abstract：:The intermittent electromagnetic fields with a large [Formula: see text] can enhance the properties of ferromagnetic materials and significantly affect paramagnetic materials. In this study, the effect of a pulsed electromagnetic field on the crystal orientation of the primary phase and microstructure evolution of an ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-67352-4

    authors： Bai Q,Wang J,Xing S,Ma Y,Bao X

    更新日期：2020-06-30 00:00:00

  • Pollen grain morphology is not exclusively responsible for pollen collectability in bumble bees.

    abstract：:Bee-pollinated plants face a dilemma in that bees both passively transport pollen grains among conspecific flowers and actively collect pollen to feed their larvae. Therefore, mechanisms that reduce pollen collection by bees have evolved in melittophilous plants. Malvaceae pollen is uncollectable for corbiculate bees ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-41262-6

    authors： Konzmann S,Koethe S,Lunau K

    更新日期：2019-03-18 00:00:00

  • Serum GFAP as a biomarker for disease severity in multiple sclerosis.

    abstract：:While neurofilament light chain (NfL) measurement in serum is a well-established marker of neuroaxonal damage in multiple sclerosis (MS), data on astroglial markers in serum are missing. In our study, glial fibrillary acid protein (GFAP) and NfL were measured in cerebrospinal fluid (CSF) and serum of MS patients and p...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-33158-8

    authors： Abdelhak A,Huss A,Kassubek J,Tumani H,Otto M

    更新日期：2018-10-04 00:00:00

  • Doping colloidal bcc crystals - interstitial solids and meta-stable clusters.

    abstract：:The addition of a small amount of dopant impurities to crystals is a common method to tune the properties of materials. Usually the doping grade is restricted by the low solubility of the dopants; increasing the doping concentration beyond this solubility limit leads to supersaturated solutions in which dopant cluster...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-12730-8

    authors： Higler R,Sprakel J

    更新日期：2017-10-03 00:00:00

  • Molecular chaperone accumulation as a function of stress evidences adaptation to high hydrostatic pressure in the piezophilic archaeon Thermococcus barophilus.

    abstract：:The accumulation of mannosyl-glycerate (MG), the salinity stress response osmolyte of Thermococcales, was investigated as a function of hydrostatic pressure in Thermococcus barophilus strain MP, a hyperthermophilic, piezophilic archaeon isolated from the Snake Pit site (MAR), which grows optimally at 40 MPa. Strain MP...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep29483

    authors： Cario A,Jebbar M,Thiel A,Kervarec N,Oger PM

    更新日期：2016-07-05 00:00:00

  • Tailoring thermal conductivity via three-dimensional porous alumina.

    abstract：:Three-dimensional anodic alumina templates (3D-AAO) are an astonishing framework with open highly ordered three-dimensional skeleton structures. Since these templates are architecturally different from conventional solids or porous templates, they teem with opportunities for engineering thermal properties. By establis...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38595

    authors： Abad B,Maiz J,Ruiz-Clavijo A,Caballero-Calero O,Martin-Gonzalez M

    更新日期：2016-12-09 00:00:00

  • Collagen morphology and texture analysis: from statistics to classification.

    abstract：:In this study we present an image analysis methodology capable of quantifying morphological changes in tissue collagen fibril organization caused by pathological conditions. Texture analysis based on first-order statistics (FOS) and second-order statistics such as gray level co-occurrence matrix (GLCM) was explored to...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep02190

    authors： Mostaço-Guidolin LB,Ko AC,Wang F,Xiang B,Hewko M,Tian G,Major A,Shiomi M,Sowa MG

    更新日期：2013-01-01 00:00:00

  • Author Correction: Magneto-Hybrid Nanofluids Flow via Mixed Convection past a Radiative Circular Cylinder.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-72921-8

    authors： El-Zahar ER,Rashad AM,Saad W,Seddek LF

    更新日期：2020-09-24 00:00:00

  • A previously unrecognized role of C3a in proteinuric progressive nephropathy.

    abstract：:Podocyte loss is the initial event in the development of glomerulosclerosis, the structural hallmark of progressive proteinuric nephropathies. Understanding mechanisms underlying glomerular injury is the key challenge for identifying novel therapeutic targets. In mice with protein-overload induced by bovine serum albu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep28445

    authors： Morigi M,Locatelli M,Rota C,Buelli S,Corna D,Rizzo P,Abbate M,Conti D,Perico L,Longaretti L,Benigni A,Zoja C,Remuzzi G

    更新日期：2016-06-27 00:00:00

  • YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus.

    abstract：:The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the proteolytic system of L. delbrue...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-09124-1

    authors： Brown L,Villegas JM,Elean M,Fadda S,Mozzi F,Saavedra L,Hebert EM

    更新日期：2017-08-17 00:00:00

  • Ilex latifolia Thunb protects mice from HFD-induced body weight gain.

    abstract：:Kuding tea is implicated in alleviating metabolic disorders in traditional Chinese medicine. However, the role of Ilex latifolia Thunb (kuding tea), one of the large leaf kuding tea species, in the prevention of the development of obesity remains to be determined. We show here that 7-week-old male mice treated with an...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-15292-x

    authors： Wu H,Chen YL,Yu Y,Zang J,Wu Y,He Z

    更新日期：2017-11-07 00:00:00

  • Functional conservation of EXA1 among diverse plant species for the infection by a family of plant viruses.

    abstract：:Since the propagation of plant viruses depends on various host susceptibility factors, deficiency in them can prevent viral infection in cultivated and model plants. Recently, we identified the susceptibility factor Essential for poteXvirus Accumulation 1 (EXA1) in Arabidopsis thaliana, and revealed that EXA1-mediated...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-42400-w

    authors： Yusa A,Neriya Y,Hashimoto M,Yoshida T,Fujimoto Y,Hosoe N,Keima T,Tokumaru K,Maejima K,Netsu O,Yamaji Y,Namba S

    更新日期：2019-04-11 00:00:00

  • Recombinant Bri3 BRICHOS domain is a molecular chaperone with effect against amyloid formation and non-fibrillar protein aggregation.

    abstract：:Molecular chaperones assist proteins in achieving a functional structure and prevent them from misfolding into aggregates, including disease-associated deposits. The BRICHOS domain from familial dementia associated protein Bri2 (or ITM2B) probably chaperones its specific proprotein region with high β-sheet propensity ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66718-y

    authors： Poska H,Leppert A,Tigro H,Zhong X,Kaldmäe M,Nilsson HE,Hebert H,Chen G,Johansson J

    更新日期：2020-06-17 00:00:00

  • Consumption of betel quid contributes to sensorineural hearing impairment through arecoline-induced oxidative stress.

    abstract：:Betel quid is one of the most widely used psychoactive substances, and is consumed by approximately 10% of the world's population. In addition to its carcinogenicity, betel quid has also been reported to affect many organs, including the brain, heart, lungs, gastrointestinal tract, and reproductive organs. As betel qu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-49815-5

    authors： Chan YH,Liu TC,Liao CK,Cheng YF,Tsai CH,Lu YC,Hu CJ,Lin HJ,Lee YL,Wu CC,Hsu CJ

    更新日期：2019-10-10 00:00:00

  • Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings.

    abstract：:Management of curable sexually-transmitted infections (STI) such as Chlamydia can be revolutionized by highly sensitive nucleic acid testing that is deployable at the point-of-care (POC). Here we report the development of a mobile nucleic acid amplification testing (mobiNAAT) platform utilizing a mobile phone and drop...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-04781-8

    authors： Shin DJ,Athamanolap P,Chen L,Hardick J,Lewis M,Hsieh YH,Rothman RE,Gaydos CA,Wang TH

    更新日期：2017-07-03 00:00:00

  • Ectopic expression of mutated type 2C protein phosphatase OsABI-LIKE2 decreases abscisic acid sensitivity in Arabidopsis and rice.

    abstract：:Abscisic acid (ABA) is a phytohormone that is necessary for stress adaptation. Recent studies have reported that attenuated levels of ABA improved grain yield and seedling growth under low temperature in cereals. To improve plant growth under low temperature, we attempted to generate ABA-insensitive transgenic rice by...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-30866-z

    authors： Endo A,Egawa C,Oohashi M,Meguro-Maoka A,Shimosaka E,Sato Y

    更新日期：2018-08-17 00:00:00

  • Forest roadsides harbour less competitive habitats for a relict mountain plant (Pulsatilla vernalis) in lowlands.

    abstract：:The long-term survival of relict populations depends on the accessibility of appropriate sites (microrefugia). In recent times, due to the mass extinction of rare species that has resulted from the loss of natural habitats, the question is - Are there any human-made sites that can act as refugial habitats? We examined...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep31913

    authors： Zielińska KM,Kiedrzyński M,Grzyl A,Rewicz A

    更新日期：2016-08-18 00:00:00

  • Adenoviral E4 34K protein interacts with virus packaging components and may serve as the putative portal.

    abstract：:Studies on dsDNA bacteriophages have revealed that a DNA packaging complex assembles at a special vertex called the 'portal vertex' and consists of a portal, a DNA packaging ATPase and other components. AdV protein IVa2 is presumed to function as a DNA packaging ATPase. However, a protein that functions as a portal is...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-07997-w

    authors： Ahi YS,Hassan AO,Vemula SV,Li K,Jiang W,Zhang GJ,Mittal SK

    更新日期：2017-08-08 00:00:00

  • Circulating microRNAs as potential diagnostic biomarkers for osteoporosis.

    abstract：:Osteoporosis is the most common age-related bone disease worldwide and is usually clinically asymptomatic until the first fracture happens. MicroRNAs are critical molecular regulators in bone remodelling processes and are stabilised in the blood. The aim of this project was to identify circulatory microRNAs associated...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-26525-y

    authors： Mandourah AY,Ranganath L,Barraclough R,Vinjamuri S,Hof RV,Hamill S,Czanner G,Dera AA,Wang D,Barraclough DL

    更新日期：2018-05-30 00:00:00