Abstract：
:Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components; however, the contribution of autophagy during meiosis has not been studied in porcine oocytes maturing in vitro. In this study, we observed that the autophagy-related gene, LC3, was expressed in porcine oocytes during maturation for 44 h in vitro. Knockdown of the autophagy-related gene, BECN1, reduced both BECN1 and LC3 protein expression levels. Moreover, BECN1 knockdown and treatment with the autophagy inhibitor, LY294002, during maturation of porcine oocytes in vitro impaired polar body extrusion, disturbed mitochondrial function, triggered the DNA damage response, and induced early apoptosis in porcine oocytes. Autophagy inhibition during oocyte maturation also impaired the further developmental potential of porcine oocytes. These results indicate that autophagy is required for the in vitro maturation of porcine oocytes.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Shen XH,Jin YX,Liang S,Kwon JW,Zhu JW,Lei L,Kim NHdoi
10.1038/s41598-018-29872-ysubject
Has Abstractpub_date
2018-08-22 00:00:00pages
12581issue
1issn
2045-2322pii
10.1038/s41598-018-29872-yjournal_volume
8pub_type
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